3 in+ovo+vaccination+site+&+homogeneity+-+building+a+deeper+understanding,+yannick+gardin,+ceva

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In-ovo vaccination site & homogeneity

Building a deeperunderstanding

Yannick Gardin, Fernando Lozano, Carlos Gonzalez, Vilmos Palya,Balasz Felfoldi, Miren Arbe, Inmaculada Hernandez, Christo Visagie,

Andras Medveczki, Eric Jaouen.Ceva Animal Health

Ceva Poultry Vaccinology SummitMarch 14th – 16th, 2016 – Barcelona, Spain

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Biology is grey

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Biology is grey

BIOLOGY

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Biology is grey

TOOL

PERFORMANCE

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Biology is grey

TOOL

HOW TO USE IT

PERFORMANCE

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Biology is grey

VACCINE

ADMINISTRATION

EFFICACY

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Biology is grey

VACCINE

ADMINISTRATION

EFFICACYFactors of variations

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More vaccinations in the hatchery

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More vaccinations in-ovo

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- Commercial broilers (20 per group)- rHVT-F (Vectormune ND) SQ or in-ovo- Challenge :

- 105 EID50 Chimalhuacan vvNDV strain- at 20 or 27or 40 days of age- by IN + ON route

- Oropharyngeal & Cloacal swabs taken 3 and 7 days post challenge- Virus quantification using RT-PCR

Validation trials in-ovo vaccination


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Clinical protection


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Reduction of shedding

* * * * * * **

Challengeat D27


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Reduction of shedding

Challengeat D40

* * * * * * **


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More vaccinations in-ovoMore questions

YG – 160316 After Wakenell et al. 2002. Avian Diseases, 46 (2) 274-280

28

More in-ovo, more questions

Injection deposition site and efficacy

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Where ?More in-ovo, more questions

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Yolk sac

Neck

SQ

Amnion

Air Cell

In-ovo injection deposition sites

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Head

Intra-Muscular

Wing


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Wing SQ

Intra-Peritoneal

Swallow

SQ

Proventriculus


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SETTING Air Chamber Allantoic Sac Yolk Sac Amniotic Sac Embryo OK Injection

1 15% 20% 0% 60% 5% 65%

2 3% 5% 0% 82% 10% 92%

No injection in the air chamber is a good indicator


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Injection not deep enough.Deposition in the air chamber.

Correct injection.No deposition in the air chamber.


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More in-ovo, more questionsWhat age ?

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18 days 19 days

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18 days 19 days

Incubation time (IT)or Embryo Development Level (EDL) ?

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18,46 18,70 18,8318,40 18,31 18,25

17,9517,56 17,69

16,00

17,00

18,00

19,00

EDL

YFT0 MDT0 OFT0 YFT1 MDT1 OFT1 YFT2 MDT2 OFT2

19 Days 18.5 Days

17.5 Days

MEDL = 17,73

MEDL = 18,32MEDL = 18,66

Incubation time (IT)or Mean Embryo Development Level (MEDL) ?

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Embryo size depends also on age of breeders

020406080

Avg. Egg Wgt Avg. Wgt. at Transfer

Avg. Egg Wgt. Loss

62 57

4,99

72 65

6,04

76 69

6,11

Young Flock Middle Age Flock Old Flock

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0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

YFT0 1/3

YFT0 2/3

YFT0 3/3

MDT0 1/3

MDT0 2/3

MDT0 3/3

OFT0 1/3

OFT0 2/3

OFT0 3/3

YFT1 1/3

YFT1 2/3

YFT1 3/3

MFT1 1/3

MFT1 2/3

MFT1 3/3

OFT1 1/3

OFT1 2/3

OFT1 3/3

YFT2 1/3

YFT2 2/3

YFT2 3/3

MFT2 1/3

MFT2 2/3

MFT2 3/3

OFT2 1/3

OFT2 2/3

OFT2 3/3

25 to 30 40 to 45 55 to 60 25 to 30 40 to 45 55 to 60 25 to 30 40 to 45 55 to 60Average INTRA EMBRYO% Average EXTRA EMBRYO% Average AIR CHAMBER+UNDEFINED%

SIS-A-2015-219 Days EDL 18.5 Days EDL 17.5 Days EDL


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MEDL 17,5 19,2Parent flock OLD YOUNGMachine tuning Regular CorruptedAir Chamber 2% 26%Allantoic 45% 0%Amnion « liquid » 53% 0%Amnion « skin & Feathers » 0% 43%Amnion « swallowed » 0% 23%IM 0% 4%SQ 0% 4%% PROTECTION / CHALLENGE 65% 10%


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Today, in-ovo injection is a very attractive option to effectively vaccinate chickens.

However, its efficacy is very much related to the qualityof the injector, its tuning, the management of the breeders, the management of the hatchery as well as the control of the vaccination process.

Conclusions

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Today, in-ovo injection is a very attractive option to effectively vaccinate chickens.

However, its efficacy is very much related to the qualityof the injector, its tuning, the management of the breeders, the management of the hatchery as well as the control of the vaccination process.

Injection should be between 18 and 19 MEDL with no injection in the airchamber

Conclusions

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In-ovo vaccination is complex and requires the supervision and support of well experienced people.These are the reasons why Ceva has developped the concept of hatchery services and the chick program, and has decided to master the development and production of in-ovo injectors.

These are key factors of success and progress.

Conclusions

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THANK YOUFOR YOUR ATTENTION

3 in+ovo+vaccination+site+&+homogeneity+-+building+a+deeper+understanding,+yannick+gardin,+ceva

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