2B-1 Introduction to Chromatography.pdf
Transcript of 2B-1 Introduction to Chromatography.pdf
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Learning Objectives
Apply the principles of chromatography
Explain the chemistry behind the
methods of analysis of compounds
Identify which method is more effective
for the analysis of compounds
Describe the methods used
in the analysis of compounds
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Analysis of Compounds
Dyes
Athletes’ blood / urine
Analysis of
Suspected illegal drugs
Cotton, wool, silk, polyester, nylon?
Unauthorised substances?
Fibres 1
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Analysis of Compounds
Identify the compound
How to separate out mixtures 1
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Find compound(s) of interest
What is it?
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Chromatography
Separate a mixture into its components
Stationary phase Inert absorbent material
Mobile phase Liquid or gas
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Chromatography
Separate a mixture into its components
Stationary phase Inert absorbent material
Mobile phase Liquid or gas
Longer experiment Greater separation
TLC plate
under
UV light
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Coloured compounds Inspection
Colourless compounds
UV light
Thin Layer Chromatography
Chemical staining
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Thin Layer Chromatography
Unknown mixture C
Standards A & B
TLC to identify compounds?
Presumptive test
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Thin Layer Chromatography
Unknown spot corresponding to standard
Compound is present
Compound may be present
Too many organic compounds
TLC plate too small