298
7
298 327 322 305 314 310 307 301 Sweden Finland Estonia Russia Latvia Baltic Sea Figure SM1: Map of part of the Baltic Sea with the sample stations in the Gulf of Finland marked.
description
Finland. 301. 310. 314. Sweden. 298. 322. 307. 305. Baltic Sea. Estonia. 327. Russia. Latvia. Figure SM1: Map of part of the Baltic Sea with the sample stations in the Gulf of Finland marked. A. B. C. D. Figure SM2 : - PowerPoint PPT Presentation
Transcript of 298
298
327
322 305
314310
307
301
Sweden
Finland
Estonia
Russia
Latvia
Baltic Sea
Figure SM1: Map of part of the Baltic Sea with the sample stations in the Gulf of Finland marked.
Figure SM2: Depth profiles for A) salinity, B) temperature, C) density and D) oxygen saturation
Temperature (OC)
0 2 4 6 8 10 12 14 16 18
Density (kg.m-3)
2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0
Dep
th (
m)
0
10
20
30
40
50
Oxygen saturation (%)
50 60 70 80 90 100 110
Salinity (psu)
4.5 5.0 5.5 6.0 6.5 7.0 7.5
Dep
th (
m)
0
10
20
30
40
50
S298S301S305S307S310S314S322S327
A B
C D
CCY0422
CCY0450
CCY0431
CCY0419
CCY0461
CCY0456
CCY0435
CCY0457
CCY0443
CCY0434
CCY0423
CCY0455
CCY0416
CCY0417
CCY0492
CCY0441
CCY0424
CCY0451
CCY0418
CCY0464
CCY0469
CCY0439
CCY0462
CCY0421
CCY0446
CCY0467
CCY0452
CCY0444
CCY0437
CCY0448
CCY0449
CCY0489
CCY0491
CCY0458
CCY0463
BSea A
CCY0466
CCY0415
CCY0436
CCY0465
BSea B
AANO01000009 Synechococcus WH 5701
AF317073 Synechococcus BO8805
97/100
100/10087/100
88/100
82/100
99/100
0.01
--/78
Figure SM3: Neighbour-joining phylogeny of group BSea isolates
Pelagic LS
BS group 3
DQ526396 Synechococcus LS0534
DQ526397 Synechococcus LS0535
CCY0444
CCY0469
CCY0443
S320-12-7
AAN01000009 Synechococcus WH5701
CCY0416
CCY0441
CCY0415
CCY0490
EF513487 Synechococcus BO8805
WH5701 Cluster/ BSea group (PC) strains
Group I (2)
DQ519960 clone LScpcBA25
PD cpcBA 1
Group A (1)
PD cpcBA 2
RS9917
CCY9201 cluster
AF223436 Synechococcus PS724
PD cpcBA 3
Group C
Group D
PS729 cluster
BSea group (32)
Group E (4)
Group B
Group H
M95288 Synechococcus WH8020 rpcBA
CP000097 Synechococcus CC9902
X59809 Synechococcus WH7803
AACY01236433 SAR- partial sequence
CP000110 Synechococcus CC9605
M95289 Synechococcus sp.
BX569694 Synechococcus WH8102 rpcBA
AAOK00000000 Synechococcus WH7805
Marine Synechococcus
Outgroups
99
98
99
99
93
99
88
99
99
99
77
99
99
97
99
57
74
73
65
89
65
9298
76
99
86
52
97
99
99
0.05
Figure SM4:Neighbour-joining phylogeny of picocyanobacterial cpcBA operon
DQ
5199
92 c
lone
LS
cpcB
A6
2D
Q52
5058
cl o
ne L
Scp
cBA
May
83
DQ
5 199
80 c
lone
LS
cpcB
A4
9D
Q51
9993
cl o
ne L
Scp
cBA
63
DQ
5200
08 c
lone
LS
cpcB
A8
8D
Q52
0009
clo
ne L
Scp
cBA
89
DQ
5200
02 c
lone
LS
cpcB
A79
DQ
5200
04 c
lone
LS
cpcB
A82
DQ
5199
91 c
lone
LS
cpcB
A61
DQ
5199
78 c
lone
LS
cpcB
A4
7D
Q51
9997
clo
ne L
Scp
cBA
71D
Q51
9957
clo
ne L
Scp
cBA
22D
Q51
9965
clo
ne L
Scp
cBA
30
DQ
5199
82 c
lone
LS
cpcB
A52
DQ
5199
53 c
lone
LS
cpcB
A18
DQ
5250
39 c
lone
LS
cpcB
AM
ay29
DQ
5199
70 c
lone
LS
cpcB
A36
DQ
5199
77 c
lone
LS
cpcB
A45
DQ
5199
90 c
lone
LS
cpcB
A60
DQ
5199
98 c
lone
LS
cpcB
A72
DQ
5250
38 c
lone
LS
cpcB
AM
ay26
DQ
5199
43 c
lone
LS
cpcB
A1
DQ
5199
84 c
lone
LS
cpcB
A54
DQ
5200
07 c
lone
LS
cpcB
A87
DQ
5199
50 c
lone
LS
cpcB
A15
DQ
5200
05 c
lone
LS
cpcB
A85
DQ
5200
03 c
lone
LS
cpcB
A80
DQ
5199
88 c
lone
LS
cpcB
A58
DQ
5199
75 c
lone
LS
cpcB
A42
DQ
5250
54 c
lone
LS
cpcB
AM
ay73
DQ
5200
06 c
lone
LS
cpcB
A86
DQ
5199
85 c
lone
LS
cpcB
A55
DQ
5250
50 c
lone
LS
cpcB
AM
ay64
DQ
5200
11 c
lone
LS
cpcB
A93
DQ
5250
48 c
lone
LS
cpcB
AM
ay61
DQ
5199
87 c
lone
LS
cpcB
A57
DQ
5199
49 c
lone
LSc
pcBA
13
DQ
5199
96 c
lone
LSc
pcBA
70
DQ
5199
55 c
lone
LSc
pcBA
20
DQ
5250
52 c
lone
LScp
cBAM
ay68
DQ52
5033
clo
ne L
ScpcB
AMay
20
DQ51
9945
clo
ne L
ScpcB
A4
DQ52
5044
clo
ne L
ScpcB
AMay
46
DQ52
5040
clo
ne L
ScpcB
AMay
34
DQ51
9994
clo
ne L
ScpcB
A64
DQ5200
01 c
lone
LScp
cBA77
DQ5199
62 c
lone
LScp
cBA27
DQ5199
64 cl
one
LScp
cBA29
DQ5199
61 cl
one
LScp
cBA26
DQ5199
63 cl
one L
ScpcB
A28
DQ519946 c
lone LScp
cBA6
DQ519948 clone LScp
cBA12
DQ520000 clone LScp
cBA74
DQ525045 clone LScp
cBAMay4
9
DQ525056 clone LScp
cBAMay7
8
DQ519954 clone LScpcBA19
DQ525041 clone LScpcBAMay40
DQ519951 clone LScpcBA16
DQ525042 clone LScpcBAMay41
DQ519968 clone LScpcBA34
DQ525030 clone LScpcBAMay13
DQ525037 clone LScpcBAMay25
DQ519959 clone LScpcBA24
DQ519979 clone LScpcBA48
DQ519944 clone LScpcBA2
DQ519986 clone LScpcBA56
DQ525031 clone LScpcBAMay16
DQ519971 clone LScpcBA37
DQ519956 clone LScpcBA21
DQ519947 clone LScpcBA11
DQ525053 clone LScpcBAMay72
DQ519974 clone LScpcBA40
DQ519972 clone LScpcBA38
DQ525032 clone LScpcBAMay17
DQ519989 clone LScpcBA59
DQ525046 clone LScpcBAMay56
DQ525051 clone LScpcBAMay65
DQ519958 clone LScpcBA23
DQ525059 clone LScpcBAMay85
DQ525043 clone LScpcBAMay42
DQ519995 clone LScpcBA66
DQ519999 clone LScpcBA73
DQ519952 clone LScpcBA17
DQ525047 clone LScpcBAMay58
DQ520010 clone LScpcBA92
DQ525049 clone LScpcBAMay63
DQ525026 clone LScpcBAMay1
DQ519967 clone LScpcBA33
DQ519976 clone LScpcBA43
DQ519966 clone LScpcBA31
DQ519973 clone LScpcBA39
DQ519981 clone LScpcBA51DQ525034 clone LScpcBAMay21DQ519983 clone LScpcBA53
DQ519969 clone LScpcBA35DQ525028 clone LScpcBAMay6DQ520013 clone LScpcBA96DQ525036 clone LScpcBAMay24DQ525029 clone LScpcBAMay9DQ525057 clone lScpcBAMay79
DQ525027 clone LScpcBAMay5DQ525055 clone LScpcBAMay75
S298-3-8S298-3-9S298-3-2S300-12-6S300-12-8
DQ526396 Synechococcus LS0534
DQ526397 Synechococcus LS0535
CCY0444CCY0469CCY0443S320-12-7AAN01000009 Synechococcus WH5701 unF
CCY0416
CCY0441CCY0415
CCY0490EF513487 Synechococcus BO8805
S314-12-9S300-3-9
S314-12-6S314-12-5S300-12-1
S314-3-8
AY151224 Synechococcus MW100C3
AY151223 Synechococcus MW97C4
AY151222 Synechococcus MW99B6
DQ526401 Synechococcus LS0530
S300-12-4S314-3-1
S314-12-4
S320-12-2
S300-3-5
S314-3-11
S300-12-3
S300-3-2
S320-3-5
S320-3-9
S300-12-7
S300-3-6
S320-3-3
S320-3-2
S320-12-3
S314-3-5
S320-12-9
S314-12-8
S314-12-11
CC
Y0468
CC
Y0432
S300-12-5
S300-12-10
S300-3-8
S300-3-3
S320-12-1
S320-3-1
S314-12-1
S314-12-7
S320-12-5
S320-3-10
S314-3-10
S300-12-9
S320-12-10
S314-3-4
S300-3-1
S300-3-7
S300-3-10
S300-3-4
S320-3-11
S320-3-7
S314-3-6
DQ
519960 clone LScpcB
A25
DQ
519885 clone P
DcpcB
A9
DQ
519893 clone PD
cpcBA
18
DQ
519897 clone P
DcpcB
A24
DQ
519913 clone P
DcpcB
A50
DQ
519912 clone P
DcpcB
A49
DQ
519938 clo
ne PD
cpcBA
89
DQ
519882 cl one P
DcpcB
A4
DQ
5 19889 clo
ne PD
cpcBA
13
DQ
519933 cl o
ne PD
cpcBA
80
AF
2234
59 Synech
ococcus PC
C6
904A
F2
23462 S
ynechococcus P
CC
7918
AF
2234
60 Synech
ococcus PC
C7
009A
Y224
201 S
ynechoco
ccus BO
8801D
Q5263
99 Synech
ococcus L
S0503
DQ
526403 S
ynechococcu
s LS
0590D
Q5264
02 Synech
ococcus LS0588
DQ
526398 Synechococcu
s LS
0417C
CY
0426S
314-3-3
S298-3-4
S298-3-7
AF
223463 Synechococcus P
CC
7920
DQ
526400 Synechococcus LS
0425
DQ
519921 clone PD
cpcBA
62D
Q519929 clone P
DcpcB
A74
DQ
519896 clone PD
cpcBA
22
DQ
519918 clone PD
cpcBA
58
DQ
519927 clone PD
cpcBA
71D
Q519906 clone P
DcpcB
A38
AA
NP
01000000 Synechococcus R
S99171 unF
AA
NP
01000000 Synechococcus R
S99172 unF
EF513488 S
ynechococcus CC
Y9201
S314-3-9
S314-12-3A
F223436 Synechococcus P
S724
DQ
519880 clone PD
cpcBA
1
DQ
519940 clone PD
cpcBA
91
DQ
519905 clone PD
cpcBA
37
DQ
525035 clone LScpcB
AM
ay23
DQ
519919 clone PD
cpcBA
59
DQ
519883 clone PD
cpcBA
5
AF223443 S
ynechococcus PS
681
DQ
519904 clone PD
cpcBA
36
AF223428 S
ynechococcus PS
680
AF223441 Synechococcus PS678
DQ
519910 clone PDcpcBA46
AF223439 Synechococcus PS676
AF223433 Synechococcus PS672
DQ519884 clone PDcpcBA7
AF223434 Synechococcus PS673
AF223438 Synechococcus PS674
DQ519890 clone PDcpcBA14
DQ519898 clone PDcpcBA25
DQ519914 clone PDcpcBA51
DQ519892 clone PDcpcBA16
DQ519942 clone PDcpcBA93
DQ519891 clone PDcpcBA15
DQ519887 clone PDcpcBA11
DQ519917 clone PDcpcBA56
DQ519886 clone P
DcpcB
A10
AF223447 Syn
echoco
ccus P
S727
AF223451 Syn
echoco
ccus P
S729
CCY0456CCY0462CCY0423CCY0492
CCY0455
CCY0452CCY0463
CCY0448CCY0417CCY0489
CCY0446CCY0491CCY0466
CCY0435CCY0439CCY0422CCY0436CCY0465CCY0421
CCY0424CCY0457CCY0449CCY0419CCY0434CCY0461CCY0418
CCY0464CCY0451
CCY0431CCY0458CCY0437CCY0467
CCY0420CCY0450
CCY0470CCY0454
EF513489 Synechococcus CCY9202
AF223449 Synechococcus PS716
AF223442 Synechococcus PS726AF223432 Synechococcus PS723
AF223445 Synechococcus PS719
AF223435 Synechococcus PS722
S320-12-6
S320-12-8
S320-3-4
S314-12-10
S320-3-6
DQ520012 clone LScpcBA95
AY151218 Synechococcus MW74D6
AY151219 Synechococcus MW73D5
AY151220 Synechococcus MW74D3
AY151217 Synechococcus MW77D1
AY151211 Synechococcus MW86A1
AY151212 Synechococcus MW10*1
AY151213 Synechococcus MW4C3
AF223437 Synechococcus PS728
AF223448 Synechococcus PS714
AY151215 Synechococcus MW15*2
AY224204 Synechococcus BO 9202
AY224202 Synechococcus BO 8807
AY224203 Synechococcus BO 8809
DQ519902 clone PDcpcBA31
AY151225 Synechococcus MW26A5
DQ519908 clone PDcpcBA42
DQ519903 clone PDcpcBA32
DQ519926 clone PDcpcBA70
DQ519937 clone PDcpcBA85
DQ519916 clone PDcpcBA55
DQ519920 clone PDcpcBA61
DQ519909 clone PDcpcBA44
DQ519911 clone PDcpcBA48
DQ519895 clone PDcpcBA21
DQ519925 clone PDcpcBA68
DQ519941 clone PDcpcBA92
DQ519899 clone PDcpcBA26
DQ519900 clone PDcpcBA27
DQ519888 clone PDcpcBA12
S298-3-5
AY151231 Synechococcus MW28B3
S298-3-1
AY151227 Synechococcus MW
33B4
AY151226 Synechococcus MW
25B5
AY151230 Synechococcus MW
32B5
AY151229 Synechococcus MW
6B6
S298-3-3
AY151228 Synechococcus MW
6C6
S298-3-6
S300-12-2
S298-3-11
M95288 Synechococcus W
H8020 rpcBA
CP000097 Synechococcus C
C9902
X59809 Synechococcus
AACY01236433 SAR
- partial sequence
CP
000110 synechococcus CC
9605
M95289 S
ynechococcus WH
8020
BX
569694 Synechococcus W
H8102 rpcB
A
AA
OK
00000000 Synechococcus W
H7805 unF
AB
008546 Synechococcus P
CC
7942
AF223464 S
ynechococcus PC
C7943
M94218 S
ynechococcus PC
C6301
K02659 S
ynechococcus PC
C7002
AF223457 S
ynechococcus PC
C6716
AF223458 S
ynechococcus PC
C6717
D13173 S
ynechococcus elongatus
CP
000239 1 Synechococcus JA
-3-3B 1
CP
000240 1 Synechococcus JA
-2-3B 1
CP
000239 2 Synechococcus JA
3-3B 2
CP
000240 2 Synechococcus JA
-2-3B 2
0.05
out-g
roup
Marine
H
B
E
BS
ea
D
A
I
WH
5701
LS-clones
Figure SM5: Neighbour-joining phylogeny of picocyanobacterial cpcBA operon
Figure SM6:
Neighbor-net network based on the 342 cpcBA operon sequences.
Marine Synechococcus
Outgroups
BSea groupPE
Lake Superior GroupBsea group
PC
Group I
Group E
Group A
18070411222.1959.13S327
1807049022.2659.24S322
1707046922.4059.30S314
1607047722.4459.30S310
1507048522.5059.30S307
1507047922.3959.28S305
1407047522.5059.32S301
1207046926.2160.04S298
DATEBottomDEPTH (m)LONGITUDELATITUDESTATION
18070411222.1959.13S327
1807049022.2659.24S322
1707046922.4059.30S314
1607047722.4459.30S310
1507048522.5059.30S307
1507047922.3959.28S305
1407047522.5059.32S301
1207046926.2160.04S298
DATEBottomDEPTH (m)LONGITUDELATITUDESTATION
Table SM1: Overview of the location, depth and the time of sampling for all Baltic Sea sampling stations.
Table SM2: Concentrations of PO43-, NO3
-, NO2-, and NH4
+ (in μM) measured at the sampling stations at the surface and at 30 m depth. Data for station 305 are lacking and therefore this station was omitted from the table.
*At station S298, deep samples were from 20 m. n.a.: not applicable
10.61n.a.1.650.060.0100.2500.180S327
5.441.501.480.110.120.010.360.030.360.10S322
6.131.221.560.110.0800.3200.320.09S314
3.882.111.580.140.290.040.460.010.600.09S310
5.251.671.880.130.180.020.4600.480.09S307
3.571.531.780.230.2000.2000.610.15S301
0.68*90.17*0.180.01*00.01*00.28*0.02S298
30m0m30m0m30m0m30m0m30m0m
N:PNH4+ (μM)NO2
- (μM)NO3- (μM)PO4
3- (μM)Stations
10.61n.a.1.650.060.0100.2500.180S327
5.441.501.480.110.120.010.360.030.360.10S322
6.131.221.560.110.0800.3200.320.09S314
3.882.111.580.140.290.040.460.010.600.09S310
5.251.671.880.130.180.020.4600.480.09S307
3.571.531.780.230.2000.2000.610.15S301
0.68*90.17*0.180.01*00.01*00.28*0.02S298
30m0m30m0m30m0m30m0m30m0m
N:PNH4+ (μM)NO2
- (μM)NO3- (μM)PO4
3- (μM)Stations
