20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.
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Transcript of 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.
![Page 1: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/1.jpg)
20x UV
20x Visible
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Pixels
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Field Illumination - 20x Objective
![Page 2: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/2.jpg)
UV Field Illumination
100X Objective (1.4 NA)
12.5% intervals
INTENSITY=230
123
47
![Page 3: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/3.jpg)
20x Vis
10x Vis (2/4/99)
10x UV
20x UV
Good
Poor
Field Illumination
![Page 4: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/4.jpg)
5x 10x 20x 40x 63x
5x 10x 20x 40x 63x
Visible
Ultraviolet
Field Illumination
![Page 5: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/5.jpg)
Axial Resolution - Lens Comparison
40x 63x 100x
610nm390nm
280nm
Axial Distance (m)
FWHMFWHM
FWHM
![Page 6: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/6.jpg)
Axial Resolution Comparison (100X Obj)
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Relative Axial Distance (m)
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Full Max Intensity = 245
Half Max = 122.5190 nm
410 nm
![Page 7: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/7.jpg)
Axial Spectral Registration - 63x Obj
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Axial Distance (m)
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647nm488nm568nm
![Page 8: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/8.jpg)
UV - 568 nm Axial Registration - 63x Obj
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0 1 2 3 4 5 6 7 8 9Axial Distance (um)
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568 nm
![Page 9: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/9.jpg)
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0 5 10 15 20 25Lateral Distance (m)
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UV
568 nm
Focal Check Beads
![Page 10: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/10.jpg)
![Page 11: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/11.jpg)
Comparison of PMT Values
![Page 12: 20x UV 20x Visible Relative Intensity Pixels Relative Intensity Field Illumination - 20x Objective.](https://reader035.fdocuments.in/reader035/viewer/2022081516/56649d615503460f94a429ea/html5/thumbnails/12.jpg)
Inte
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Axial Profile
Mirror - Visible Beads - UV/Visible Focal Check Beads
Spectral Registration Check Methods