2019~2020 - nanochrom.comScalability and consistency Unique surface chemistry and ... L20 N/A L68...

NanoChrom Technologies (Suzhou) Co., Ltd

2019~2020

ChromatographyConsumablesProduct CatalogAddress：

Phone：

Web-Site ：

E-mail ：

Contact Us398 Ruoshui Road, Suzhou Industrial

Park, Jiangsu, China

+86-0512-62626680

www.nanochrom.com

[email protected]

Trusted partner and innovation leader in chromatography

India Subsidiary

Suzhou Nanomicro Tech India Private Limited

Address: 1033, Tower A, Ithum Building, plot-A40

Noida Sector 62, Gautam Buddha Nagar

Uttar Pradesh 201301, INDIA

Phone: +91 96548 80558

E-mail: [email protected]

Contents��

NanoChrom Company

Our products are based on UniSil® and UniCore® technologies - the world's most advanced monodis-persed particle technology at industrial scale, ensuring high performance and consistency. Com-bined with the state-of-the-art surface modification technology, many highly desired products can be developed to meet our customer's need.

Our technical team consists of world class experts in liquid chromatography，synthetic chemistry and materials science. We also have a highly experi-enced management team with strong leadership and vision to serve our customers.

NanoChrom Technologies (NanoChrom) is a technology driven company, specialized in research, development, manufacturing and service of chromatography consumables for applications in a broad range of industries including pharmaceutical, biotechnology, food beverage, environmental, chemical, etc.

NanoChrom offers a comprehensive product portfolio, ranging from HPLC columns for small molecules and bio-separation columns for proteins and oligos to chiral columns for enantiomer separations and solid phase extraction (SPE) for sample preparation. We are keen to work with our customers in developing targeted solutions to various separation challenges.

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ChromCoreTM HPLC Columns P03

BioCoreTM HPLC ColumnsP22

EcoPakTM HPLC Columns P21

SelectCoreTM SPE Columns P33

UniChiral® HPLC Columns P30

P2-PNanoChrom BPTM GC Columns P41

Contents��

NanoChrom Company

Our products are based on UniSil® and UniCore® technologies - the world's most advanced monodis-persed particle technology at industrial scale, ensuring high performance and consistency. Com-bined with the state-of-the-art surface modification technology, many highly desired products can be developed to meet our customer's need.

Our technical team consists of world class experts in liquid chromatography，synthetic chemistry and materials science. We also have a highly experi-enced management team with strong leadership and vision to serve our customers.

NanoChrom Technologies (NanoChrom) is a technology driven company, specialized in research, development, manufacturing and service of chromatography consumables for applications in a broad range of industries including pharmaceutical, biotechnology, food beverage, environmental, chemical, etc.

NanoChrom offers a comprehensive product portfolio, ranging from HPLC columns for small molecules and bio-separation columns for proteins and oligos to chiral columns for enantiomer separations and solid phase extraction (SPE) for sample preparation. We are keen to work with our customers in developing targeted solutions to various separation challenges.

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ChromCoreTM HPLC Columns P03

BioCoreTM HPLC ColumnsP22

EcoPakTM HPLC Columns P21

SelectCoreTM SPE Columns P33

UniChiral® HPLC Columns P30

P2-PNanoChrom BPTM GC Columns P41

Advanced Bonding Chemistry

The Core of NanoChrom Liquid Chromatography ProductsUniSil® and UniCore® Monodispersed Spherical Particles

NanoChrom's products are based on the latest advancement in particle technology ‒ monodispersed spherical UniSil silica particles and UniCore polymer particles. These innovative particle technologies feature unprecedented level of control over particle size, pore structure and purity, marking a milestone in separation media for liquid chromatogra-phy.

UniSil silica particles with broad selection range with precise control on particle size (from 1.7μm to 50μm).

Metal contamination affects chromatography performance, including peak tailing for basic compounds. UniSil particles are manufactured using high-purity, metal -free silanes as starting materials and a well-developed production process, resulting in extremely low metal contamination.

Undetected

Metal Detection Limit (mg/kg） Result (mg/kg）

UniSil silica particles exhibit monodispersed particle distribution ‒ superior to any other competitors.

UniSil silica particles with precise control on pore size (from 60 to 1600 Å).

Particle size distribution comparison - UniSil vs Competitors'

Physical strength comparison - UniSil vs Competitors

Particle size distribution comparison - UniSil vs Competitors' (SEM image)

Irregular Silica Spherical Silica Monodispersed Spherical Silica (Developed by NanoMicro Technology)

1960 1980 2016

Monodispersed particles for HPLC and UHPLC

Precise control over particle size and pore structure

UniSil silica particles show excellent physical strength as the result of its advanced particle manufacture process. As shown below, after being packed 20 times at high pressure, no visible particle breakage is observed under SEM, while under the same condition, significant broken particles can be observed on several commonly used competitors' particles.

Excellent Physical Strength

NanoChrom LC columns adopt the state-of-the-art surface chemistry, covering a broad selectivity range, including reversed phase (such as C18，AQ，C8，C30，PFP，Phenyl, Biphenyl, etc), normal phase (such as Silica, Amino and Cyano)and HILIC (Diol, Amide, Imidazole), to meet a variety of separation challenges.

High Purity

Monodispersed Particle Size

UniSil

Silica Particles

Monodispersed spherical particles（CV≤3%）

Outstanding physical strength

Wide selection of particle size: 1.7~50 µm; pore size: 60~2000 Å

High column efficiency: 100,000+ plates/m (5µm)

Excellent chemical stability

Extremely low metal contect

Scalability and consistency

Unique surface chemistry and broad range of selectivity

2μm 5μm 10μm 20μm 30μm 50μm

60 Å 100 Å 200 Å

500 Å 800 Å 1600 Å

Advanced Bonding Chemistry

The Core of NanoChrom Liquid Chromatography ProductsUniSil® and UniCore® Monodispersed Spherical Particles

NanoChrom's products are based on the latest advancement in particle technology ‒ monodispersed spherical UniSil silica particles and UniCore polymer particles. These innovative particle technologies feature unprecedented level of control over particle size, pore structure and purity, marking a milestone in separation media for liquid chromatogra-phy.

UniSil silica particles with broad selection range with precise control on particle size (from 1.7μm to 50μm).

Metal contamination affects chromatography performance, including peak tailing for basic compounds. UniSil particles are manufactured using high-purity, metal -free silanes as starting materials and a well-developed production process, resulting in extremely low metal contamination.

Undetected

Metal Detection Limit (mg/kg） Result (mg/kg）

UniSil silica particles exhibit monodispersed particle distribution ‒ superior to any other competitors.

UniSil silica particles with precise control on pore size (from 60 to 1600 Å).

Particle size distribution comparison - UniSil vs Competitors'

Physical strength comparison - UniSil vs Competitors

Particle size distribution comparison - UniSil vs Competitors' (SEM image)

Irregular Silica Spherical Silica Monodispersed Spherical Silica (Developed by NanoMicro Technology)

1960 1980 2016

Monodispersed particles for HPLC and UHPLC

Precise control over particle size and pore structure

UniSil silica particles show excellent physical strength as the result of its advanced particle manufacture process. As shown below, after being packed 20 times at high pressure, no visible particle breakage is observed under SEM, while under the same condition, significant broken particles can be observed on several commonly used competitors' particles.

Excellent Physical Strength

NanoChrom LC columns adopt the state-of-the-art surface chemistry, covering a broad selectivity range, including reversed phase (such as C18，AQ，C8，C30，PFP，Phenyl, Biphenyl, etc), normal phase (such as Silica, Amino and Cyano)and HILIC (Diol, Amide, Imidazole), to meet a variety of separation challenges.

High Purity

Monodispersed Particle Size

UniSil

Silica Particles

Monodispersed spherical particles（CV≤3%）

Outstanding physical strength

Wide selection of particle size: 1.7~50 µm; pore size: 60~2000 Å

High column efficiency: 100,000+ plates/m (5µm)

Excellent chemical stability

Extremely low metal contect

Scalability and consistency

Unique surface chemistry and broad range of selectivity

2μm 5μm 10μm 20μm 30μm 50μm

60 Å 100 Å 200 Å

500 Å 800 Å 1600 Å

ChromCore columns, consisting of reversed-phase (RP), normal phase (NP)/hydrophilic interaction (HILIC) and application-specific columns, are designed for a broad range of applications in pharmaceutical, chemical, environmental, food & beverage, research, etc.

The high performance of ChromCore columns is the result of innovative particle technology, advanced surface chemis-try and reproducible manufacturing processes. ChromCore separation media are based on monodispersed particles with precisely controlled particle size and pore structure, as well as high mechanical strength, providing high efficiency and consistency. Advanced surface chemistry results in excellent chromatography properties with desired selectivity, making ChromCore columns suited for abroad application range. ChromCore columns are produced using well-devel-oped manufacturing processes under strict quality control, ensuring high quality and reproducibility.

ChromCoreTM HPLC Columns

ChromCore HPLC Columns Parameter information

120 C18

300 C18

AQ C18

AR C18

Polar C18

120 C8

300 C8

300 C4

300 C4-T

Phenyl

Biphenyl

Silica

HILIC-Diol

HILIC-Imidazole

HILIC-Amide

1.8, 3, 5

Bonded Phase Carbon Load ( %) pH Particle size（μm）Pore size（Å） SSa（m2/g） USP Listing

ChromCoreTM Reversed-Phase LC ColumnsChromCore C18

ChromCore AQ C18

ChromCore Polar C18

ChromCore AR C18

ChromCore C8

ChromCore AQ C8

ChromCore C4

ChromCore C30

ChromCore Phenyl

ChromCore BiphenylChromCore PFP

ChromCoreTM Normal Phase/HILIC LC ColumnsChromCore Silica

ChromCore NH2

ChromCore CN

ChromCore HILIC-Diol

ChromCore HILIC-Imidazole

ChromCoreTM Sugar Columns

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17--------------------------------------------- 17

ChromCore HILIC-Amide

-------------------------------------

ChromCoreTM Preparative Columns 20-------------------------------------

ChromCore columns, consisting of reversed-phase (RP), normal phase (NP)/hydrophilic interaction (HILIC) and application-specific columns, are designed for a broad range of applications in pharmaceutical, chemical, environmental, food & beverage, research, etc.

The high performance of ChromCore columns is the result of innovative particle technology, advanced surface chemis-try and reproducible manufacturing processes. ChromCore separation media are based on monodispersed particles with precisely controlled particle size and pore structure, as well as high mechanical strength, providing high efficiency and consistency. Advanced surface chemistry results in excellent chromatography properties with desired selectivity, making ChromCore columns suited for abroad application range. ChromCore columns are produced using well-devel-oped manufacturing processes under strict quality control, ensuring high quality and reproducibility.

ChromCore HPLC Columns Parameter information

120 C18

300 C18

AQ C18

AR C18

Polar C18

120 C8

300 C8

300 C4

300 C4-T

Phenyl

Biphenyl

Silica

HILIC-Diol

HILIC-Imidazole

HILIC-Amide

1.8, 3, 5

Bonded Phase Carbon Load ( %) pH Particle size（μm）Pore size（Å） SSa（m2/g） USP Listing

ChromCoreTM Reversed-Phase LC ColumnsChromCore C18

ChromCore AQ C18

ChromCore Polar C18

ChromCore AR C18

ChromCore C8

ChromCore AQ C8

ChromCore C4

ChromCore C30

ChromCore Phenyl

ChromCore BiphenylChromCore PFP

ChromCoreTM Normal Phase/HILIC LC ColumnsChromCore Silica

ChromCore NH2

ChromCore CN

ChromCore HILIC-Diol

ChromCore HILIC-Imidazole

ChromCoreTM Sugar Columns

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17--------------------------------------------- 17

ChromCore HILIC-Amide

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ChromCoreTM Preparative Columns 20-------------------------------------

ChromCore reversed-phase (RP) columns are designed for separating compounds of various hydrophobicity, making them the columns of choice for a broad range of applications. The column chemistries include C18, C8, C4, C30, AQ, Polar, Phenyl, PFP, and Biphenyl, providing a wide selectivity range.

Advanced bonding technology based on monodispersed-high-purity spherical silica greatly minimizes silanol activity thus inproves peak shape symmetry for basic analyte.

Compared with a commercial C18 column based on conventional silica particles, ChromCore C18 demonstrates significantly enhanced chemical stability in alkaline conditions as the result of its high pH resistance nature of base particle and the protection of densely bonded surface.

The combination of advanced monodispersed paticle technology and innovative chemistry leads to higher column efficiency compared to competing products in the market.

The unique column chemistry ensures excellent compatibility with highly aqueous mobile phase.

Column Efficiency

Aqueous Compatibility

Peak ShapeChromCore Reversed-Phase LC Columns

ChromCore C18, 5 μm4.6x250 mm60/40 v/v MeCN/D.I. H2O1 mL/min30 ℃5 μLUV 254 nmNaphthelene

Column :Dimension :Mobile phase :Flow rate :Temperature :Injection :Detection :Analyte :

Column :Dimension :Mobile phase :

Flow rate :Temperature :Injection :Detection :Peaks：

ChromCore C18, 5 μm4.6x250 mm80/20 v/v MeOH/20 mMphosphate buffer, pH 71 mL/min30 ℃5 μLUV 254 nm1. Uracil2. Toluene3. Ethylbenzene4. Quinizarin5. Amitriptyline

ChromCore C18, 5 μm4.6x150 mm10mM ammonium acetate, pH5.21 mL/min30 ℃5 μLUV 254 nm1. Cytosine2. Uracil3. Thymine

Column:Dimension:Mobile phase:

Flow rate:Temperature:Injection:Detection:Analyte:

Stress Condition:Mobile phase:Flow rate:Temperature:

pH Stability

Column

ChromCore C18

Competitor P C18

Competitor A C18

Competitor W C18

AmitriptylineAsymmetry(normalized）

QuinizarinAsymmetry(normalized)

Main features

Advanced monodispered particle technology for high efficiency, mass transfer and mechanical

strength

Versatile column chemistry for broad selectivity coverage

Excellent chromatography performance: symmetrical peaks for acids, bases and neutrals

Low column bleed for MS compatibility

Good column-to-column consistency

ChromCore C18, 5 μm4.6×150 mm10/90 v/v MeCN/10 mM ammonium acetate, pH 5.21 mL/min30 ℃5 μLUV 225 nmAcetanilide (0.1 mg /mL)

100 mM NaOH1 mL/min30 ℃

2743024828

2141024885

ChromCore C18 Compe�tor P C18 Compe�tor A C18 Compe�tor W C18

Efficie

0 2 4 6 8 10 12 14 16 18 20

n�on

Stop Flow (cycle)

Cytosine Uracil Thymine

0.0 5.0 10.0 15.0 20.0

Time [min]

1 2 34 5

1 2 3 45

ChromCore C18

Competitor P C18

Competitor A C18

Competitor W C18

0 2 4 6 8 10 12 14 16 18 20

n Effi

yExposure Time at pH13 (hour)

ChromCore C18 Compe�tor C18 (interna�onal brand)

小分

子分

离液

相色

谱柱

ChromCore reversed-phase (RP) columns are designed for separating compounds of various hydrophobicity, making them the columns of choice for a broad range of applications. The column chemistries include C18, C8, C4, C30, AQ, Polar, Phenyl, PFP, and Biphenyl, providing a wide selectivity range.

Advanced bonding technology based on monodispersed-high-purity spherical silica greatly minimizes silanol activity thus inproves peak shape symmetry for basic analyte.

Compared with a commercial C18 column based on conventional silica particles, ChromCore C18 demonstrates significantly enhanced chemical stability in alkaline conditions as the result of its high pH resistance nature of base particle and the protection of densely bonded surface.

The combination of advanced monodispersed paticle technology and innovative chemistry leads to higher column efficiency compared to competing products in the market.

The unique column chemistry ensures excellent compatibility with highly aqueous mobile phase.

Column Efficiency

Aqueous Compatibility

Peak ShapeChromCore Reversed-Phase LC Columns

ChromCore C18, 5 μm4.6x250 mm60/40 v/v MeCN/D.I. H2O1 mL/min30 ℃5 μLUV 254 nmNaphthelene

Column :Dimension :Mobile phase :Flow rate :Temperature :Injection :Detection :Analyte :

Flow rate :Temperature :Injection :Detection :Peaks：

ChromCore C18, 5 μm4.6x250 mm80/20 v/v MeOH/20 mMphosphate buffer, pH 71 mL/min30 ℃5 μLUV 254 nm1. Uracil2. Toluene3. Ethylbenzene4. Quinizarin5. Amitriptyline

ChromCore C18, 5 μm4.6x150 mm10mM ammonium acetate, pH5.21 mL/min30 ℃5 μLUV 254 nm1. Cytosine2. Uracil3. Thymine

Column:Dimension:Mobile phase:

Flow rate:Temperature:Injection:Detection:Analyte:

Stress Condition:Mobile phase:Flow rate:Temperature:

pH Stability

Column

ChromCore C18

Competitor P C18

Competitor A C18

Competitor W C18

AmitriptylineAsymmetry(normalized）

QuinizarinAsymmetry(normalized)

Main features

Advanced monodispered particle technology for high efficiency, mass transfer and mechanical

strength

Versatile column chemistry for broad selectivity coverage

Excellent chromatography performance: symmetrical peaks for acids, bases and neutrals

Low column bleed for MS compatibility

Good column-to-column consistency

ChromCore C18, 5 μm4.6×150 mm10/90 v/v MeCN/10 mM ammonium acetate, pH 5.21 mL/min30 ℃5 μLUV 225 nmAcetanilide (0.1 mg /mL)

100 mM NaOH1 mL/min30 ℃

2743024828

2141024885

ChromCore C18 Compe�tor P C18 Compe�tor A C18 Compe�tor W C18

Efficie

0 2 4 6 8 10 12 14 16 18 20

n�on

Stop Flow (cycle)

Cytosine Uracil Thymine

0.0 5.0 10.0 15.0 20.0

Time [min]

1 2 34 5

1 2 3 45

ChromCore C18

Competitor P C18

Competitor A C18

Competitor W C18

0 2 4 6 8 10 12 14 16 18 20

n Effi

Exposure Time at pH13 (hour)

ChromCore C18 Compe�tor C18 (interna�onal brand)

小分

子分

离液

相色

谱柱

ChromCore C18 columns are based on high surface coverage C18 modified silica particles with exhaustive end-capping for minimal undesired silanol activity. They are designed as most commonly used columns for separating analytes with low to high hydrophobicity.

ChromCoreTM C18

Column: Dimension: Mobile phase:

Gradient:

Flow rate: Temperature:Injection:Detection: Sample:

ChromCore 120 C18, 3 μm2.1×150 mmA) 0.1% HCOOH in H2OB) 0.1% HCOOH in MeCNt (min) A B-10 98 20 98 215 60 4020 60 400.21 mL/min30 ℃5 μLUV 210 nm8 pmol/µL Cytochrome C Digest

Cytochrome C Digest

Applications

Column :Dimension :Mobile phase :Gradient :

Flow rate :Temperature :Injection :Detection :Samples :

ChromCore C18, 5 μm4.6x250 mmA, 0.1% HCOOH in MeCNB, 0.1% HCOOH in H2Ot (min) A B -5 30 70 0 30 70 20 95 51 mL/min30 ℃5 μLUV 248 nm1. Pravastatin 2. Lovastatin 3. Simvastatin

Satins

ChromCore C18, 5 μm4.6x150 mmA, MeCN B, H2Ot (min) A B -5 35 65 0 35 65 10 80 201 mL/min30 ℃5 μLUV 220 nm1. Estriol 2. α-Estradiol 3. Ethinyl Estradiol 4. Levonorgestrel

Estrogen

6 15 19.5 24

Time [min]

0 2 4 6 8 10 12 14 16 18 20Time [min]

0 1 2 3 4 5 6 7 8 9 10Time [min]

Column: Dimension:Mobile phase: Gradient:

Flow rate:Temperature:Injection:MS:Sample:

ChromCore C18, 5 μm4.6×150 mmA）50/50 v/v MeOH/MeCNB）50mM Na2HPO4 aq. pH7.8t (min) A B 0 3 97 2 3 97 16 22 78 30 35 65 38 70 301.5 mL/min35 ℃2 μLUV 338 nm1. Asp 2. Background 3. Glu 4. Ser 5. His 6. Gly7. Thr 8. Arg 9. Background10. Ala 11.Tyr 12. Background13. Val 14. Cys 15. Met 16. Phe 17. Ile 18. Leu 19. Lys

Amino Acids

Time [min]2 9 16 23

1 2 3 4

16 17 18

Reference Substance

Column :Dimension :Mobile phase :Flow rate :Temperature :Injection :Detection :Samples:

ChromCore C18, 5 μm4.6x250 mm80/20 v/v MeOH/10 mM NH4HCO3, pH101 mL/min30 ℃5 μLUV 220 nm1. Desipramine 2. Doxepin 3. Nortriptyline 4. Imipramine 5. Amitriptyline 6. Trimipramine

Tetracyclic Antidepressants

0 2 4 6 8 10 12 14 16 18 20Time [min]

ChromCore 120 C18, 3 μm4.6x150 mmA, MeCN B, H2Ot (min) A B -5 40 60 0 40 60 10 80 201 mL/min25 ℃5 μLUV 245 nm1. Tebuthiuron 2. Thidiazuron3. Monuron (Surr) 4. Fluometuron5. Diuron 6. Propanil & Siduron A7. Siduron B 8. Liunuron9. Carbazole (Surr) 10. Diflubenzuron

ChromCore C18, 5 μm4.6x250 mmA, MeOH B, H2Ot (min) A B -5 70 30 0 70 30 18 100 0 20 100 01 mL/min30 ℃5 μLUV 360 nm1. Formaldehyde 2. Acetaldehyde 3. Propanal 4. Crotonaldehyde 5. Butanal 6. Cyclohexanone 7. Pentanal 8. Hexanal 9. Heptanal 10. Octanal 11. Nonanal 12. Decanal

Carbonyl (US EPA 554)

Phenyl Urea （US EPA 532）

0 2.5 5 7.5 10Time [min]

0 2 4 6 8 10 12 14 16 18 20

Time [min]

8 9 10

ChromCore C18 columns are based on high surface coverage C18 modified silica particles with exhaustive end-capping for minimal undesired silanol activity. They are designed as most commonly used columns for separating analytes with low to high hydrophobicity.

ChromCoreTM C18

Column: Dimension: Mobile phase:

Gradient:

Flow rate: Temperature:Injection:Detection: Sample:

ChromCore 120 C18, 3 μm2.1×150 mmA) 0.1% HCOOH in H2OB) 0.1% HCOOH in MeCNt (min) A B-10 98 20 98 215 60 4020 60 400.21 mL/min30 ℃5 μLUV 210 nm8 pmol/µL Cytochrome C Digest

Cytochrome C Digest

Applications

ChromCore C18, 5 μm4.6x250 mmA, 0.1% HCOOH in MeCNB, 0.1% HCOOH in H2Ot (min) A B -5 30 70 0 30 70 20 95 51 mL/min30 ℃5 μLUV 248 nm1. Pravastatin 2. Lovastatin 3. Simvastatin

Satins

ChromCore C18, 5 μm4.6x150 mmA, MeCN B, H2Ot (min) A B -5 35 65 0 35 65 10 80 201 mL/min30 ℃5 μLUV 220 nm1. Estriol 2. α-Estradiol 3. Ethinyl Estradiol 4. Levonorgestrel

Estrogen

6 15 19.5 24

Time [min]

0 2 4 6 8 10 12 14 16 18 20Time [min]

0 1 2 3 4 5 6 7 8 9 10Time [min]

ChromCore C18, 5 μm4.6×150 mmA）50/50 v/v MeOH/MeCNB）50mM Na2HPO4 aq. pH7.8t (min) A B 0 3 97 2 3 97 16 22 78 30 35 65 38 70 301.5 mL/min35 ℃2 μLUV 338 nm1. Asp 2. Background 3. Glu 4. Ser 5. His 6. Gly7. Thr 8. Arg 9. Background10. Ala 11.Tyr 12. Background13. Val 14. Cys 15. Met 16. Phe 17. Ile 18. Leu 19. Lys

Amino Acids

Time [min]2 9 16 23

1 2 3 4

16 17 18

Reference Substance

Column :Dimension :Mobile phase :Flow rate :Temperature :Injection :Detection :Samples:

ChromCore C18, 5 μm4.6x250 mm80/20 v/v MeOH/10 mM NH4HCO3, pH101 mL/min30 ℃5 μLUV 220 nm1. Desipramine 2. Doxepin 3. Nortriptyline 4. Imipramine 5. Amitriptyline 6. Trimipramine

Tetracyclic Antidepressants

0 2 4 6 8 10 12 14 16 18 20Time [min]

ChromCore 120 C18, 3 μm4.6x150 mmA, MeCN B, H2Ot (min) A B -5 40 60 0 40 60 10 80 201 mL/min25 ℃5 μLUV 245 nm1. Tebuthiuron 2. Thidiazuron3. Monuron (Surr) 4. Fluometuron5. Diuron 6. Propanil & Siduron A7. Siduron B 8. Liunuron9. Carbazole (Surr) 10. Diflubenzuron

ChromCore C18, 5 μm4.6x250 mmA, MeOH B, H2Ot (min) A B -5 70 30 0 70 30 18 100 0 20 100 01 mL/min30 ℃5 μLUV 360 nm1. Formaldehyde 2. Acetaldehyde 3. Propanal 4. Crotonaldehyde 5. Butanal 6. Cyclohexanone 7. Pentanal 8. Hexanal 9. Heptanal 10. Octanal 11. Nonanal 12. Decanal

Carbonyl (US EPA 554)

Phenyl Urea （US EPA 532）

0 2.5 5 7.5 10Time [min]

0 2 4 6 8 10 12 14 16 18 20

Time [min]

8 9 10

0 6 12 24

Time [min]Column: Dimension:Mobile phase: Gradient:

ChromCore 120 C18, 3 μm2.1×150 mmA) 0.1% HCOOH in H2OB) 0.1% HCOOH in MeCNt (min) A B -10 98 2 0 98 2 1 98 2 40 60 40 41 5 95 45 5 95 46 98 20.2 mL/min40 ℃3 μLQE PlusBSA Digest（5 pmol/μL）

BSA Digest ‒ MS

ChromCore 120 C18, 3 μm4.6x150 mmA, MeCNB, 0.1% H3PO4in H2Ot (min) A B -5 30 70 0 30 70 15 90 101 mL/min40 ℃5 μLUV 214 nm1. Phenol 2. 4-Nitrophenol 3. 2-Chlorophenol 4. 2-Nitrophenol5. 2,4-Dimethylphenol 6. 2,4-Dinitrophenol7. 4-Chloro-3-methylphenol 8. 2,4-Dichloropheol 9. 2-Methyl-4,6-dichlorophenol10. 2,4,6-Trichlorophenol 11. Pentachlorophenol

ChromCore 120 C18, 3 μm4.6x150 mmA, MeCN B, H2Ot (min) A B -5 65 35 0 65 35 80 20 2 4 100 01 mL/min30 ℃5 μLUV 210 nm1. Dimethyl phthalate2. Diethyl phthalate3. Di-n-butyl phthalate4. Butyl benzyl phthalate5. Bis(2-ethylhexyl) phthalate6. Di-n-octyl phthalate

Phenols (US EPA 604) Phthalate Esters（US EPA 606）

Gradient :

ChromCore 120 C18, 3 μm4.6×150 mmA, 0.1% HCOOH in MeCNB, 0.1% HCOOH in H2Ot (min) A B -5 5 95 0 5 95 15 80 20 18 80 201 mL/min30 ℃5 μLUV 265 nm1. Acetaminophen 2. Chlorpheniramine 3. Dextromethorphan 4. Diphenhydramine 5. Ibuprofenl

Cold Medicine

ChromCore 120 C18, 5 μm4.6×250 mm10/90 v/v MeOH/0.04% Sodium pentane sulfonate solution ，pH3.01 mL/min30 ℃10 μLUV 291 nm1. Vitamin B62. Vitamin B6 Impurity A3. Vitamin B6 Impurity B

Vitamin B6

Gradient :

ChromCore 120 C18, 3 mm4.6×150 mmA）0.1% HCOOH in MeCNB）0.1% HCOOH in H2Ot (min) A B -5 5 95 0 5 95 15 50 501 mL/min30 oC5 μLUV 254 nm1. Procaine 2. Lidocaine3. Bupivacaine 4. Butacaine5. Tetracaine 6. Benzocaine7. Dibucaine

Local Anaesthetics

Gradient :Temperature :Injection :Detection :Samples :

ChromCore 120 C18, 5 μm4.6×250 mm96/4 v/v 1.4 mM Tetrabutylammonium hydrogen sulfate & 10 mM KH2PO4 pH2.5/MeOH1.0 mL/min25 ℃10 μLUV 254 nm1. CMP2. AMP3. UMP4. GMP5. IMP

Five nucleotides in milk powder

0 5 10 15 20 25 30 35 40 45 50Time [min]

System suitability

System suitability +VB6

0 6 12 15

Time [min]

0 5 10 15 20 25 30 35 40

Time (min)

0 2 4 6 8 10 12 14 15

Time [min]

2 3 4 5

0 2 4 6 8 10 12 14 16 18

Time [min]

0 1 2 3 4 5 6 7 8 9 10Time [min]

0 6 12 24

Time [min]Column: Dimension:Mobile phase: Gradient:

ChromCore 120 C18, 3 μm2.1×150 mmA) 0.1% HCOOH in H2OB) 0.1% HCOOH in MeCNt (min) A B -10 98 2 0 98 2 1 98 2 40 60 40 41 5 95 45 5 95 46 98 20.2 mL/min40 ℃3 μLQE PlusBSA Digest（5 pmol/μL）

BSA Digest ‒ MS

ChromCore 120 C18, 3 μm4.6x150 mmA, MeCNB, 0.1% H3PO4in H2Ot (min) A B -5 30 70 0 30 70 15 90 101 mL/min40 ℃5 μLUV 214 nm1. Phenol 2. 4-Nitrophenol 3. 2-Chlorophenol 4. 2-Nitrophenol5. 2,4-Dimethylphenol 6. 2,4-Dinitrophenol7. 4-Chloro-3-methylphenol 8. 2,4-Dichloropheol 9. 2-Methyl-4,6-dichlorophenol10. 2,4,6-Trichlorophenol 11. Pentachlorophenol

ChromCore 120 C18, 3 μm4.6x150 mmA, MeCN B, H2Ot (min) A B -5 65 35 0 65 35 80 20 2 4 100 01 mL/min30 ℃5 μLUV 210 nm1. Dimethyl phthalate2. Diethyl phthalate3. Di-n-butyl phthalate4. Butyl benzyl phthalate5. Bis(2-ethylhexyl) phthalate6. Di-n-octyl phthalate

Phenols (US EPA 604) Phthalate Esters（US EPA 606）

Gradient :

ChromCore 120 C18, 3 μm4.6×150 mmA, 0.1% HCOOH in MeCNB, 0.1% HCOOH in H2Ot (min) A B -5 5 95 0 5 95 15 80 20 18 80 201 mL/min30 ℃5 μLUV 265 nm1. Acetaminophen 2. Chlorpheniramine 3. Dextromethorphan 4. Diphenhydramine 5. Ibuprofenl

Cold Medicine

ChromCore 120 C18, 5 μm4.6×250 mm10/90 v/v MeOH/0.04% Sodium pentane sulfonate solution ，pH3.01 mL/min30 ℃10 μLUV 291 nm1. Vitamin B62. Vitamin B6 Impurity A3. Vitamin B6 Impurity B

Vitamin B6

Gradient :

ChromCore 120 C18, 3 mm4.6×150 mmA）0.1% HCOOH in MeCNB）0.1% HCOOH in H2Ot (min) A B -5 5 95 0 5 95 15 50 501 mL/min30 oC5 μLUV 254 nm1. Procaine 2. Lidocaine3. Bupivacaine 4. Butacaine5. Tetracaine 6. Benzocaine7. Dibucaine

Local Anaesthetics

Gradient :Temperature :Injection :Detection :Samples :

ChromCore 120 C18, 5 μm4.6×250 mm96/4 v/v 1.4 mM Tetrabutylammonium hydrogen sulfate & 10 mM KH2PO4 pH2.5/MeOH1.0 mL/min25 ℃10 μLUV 254 nm1. CMP2. AMP3. UMP4. GMP5. IMP

Five nucleotides in milk powder

0 5 10 15 20 25 30 35 40 45 50Time [min]

System suitability

System suitability +VB6

0 6 12 15

Time [min]

0 5 10 15 20 25 30 35 40

Time (min)

0 2 4 6 8 10 12 14 15

Time [min]

2 3 4 5

0 2 4 6 8 10 12 14 16 18

Time [min]

0 1 2 3 4 5 6 7 8 9 10Time [min]

Flow rate:Temperature:Injection:Detection:Sample: Peaks:

ChromCoreTMAQ C18, 5 μm4.6×250mmA) H20B) MeOHt (min) A B -10 28 72 0 28 72 5.7 28 72 8.0 20 80 15.0 20 80 20.0 28 721 mL/minRoom temperature5 μLUV 290 nmSidenafil Mixed standard solution1. Aminotadalafil 2. N-Demethylsildenafil3. Sildenafil Citrate4. Homo Sildenafil 5. Demethylthiophane6. Thiohamoxdenafil

Sildenafil in urine sample

ChromCore AQ C18 columns are based on proprietary C18 modified silica particles for excellent aqueous compatibility. They are the column of choice for applications requiring highly aqueous mobile phase and/or C18 selectivity.

ChromCoreTM AQ C18

Column Black: ChromCoreTM AQ C18，5µm Column Blue: Agilent TC-C18，5µm Dimension: 4.6×250mmMobile phase: 70/30 v/v H2O/MeCNFlow rate: 1 mL/minTemperature: Room temperatureInjection: 25 μLDetection: UV 220 nmSample: 5 ppm Glycyl-L-Histidyl-L-Lysine

Glycyl-L-Histidyl-L-Lysine

ChromCore Agilent

Applications

ChromCore C4 columns are based on high surface coverage C4 modified silica particles with exhaustive end-capping. They are designed for separating analytes of high hydrophobicity.

ChromCoreTM C4

Retention Time

Peak area

Peak Height

Symmetry

Theoretical plates

7.867min

12.427

7.707min

10.364

0 9 13.5 18Time [min]

8ppm Standard

4ppm Standard in sample

0 9 12 15Time [min]

ChromCore AQ C18

Column: Dimension:Mobile phase:

Gradient:

Flow rate:Injection:Temperature:Detection:Sample:

ChromCoreTM120 C8 5 µm4.6×150 mmA）MeCNB）IPAC）0.1 M Acetic acid, pH 5.0t (min) A B C-10 90 5 50 90 5 530 0 95 540 0 95 51.0 mL/min5 µL30oCCADCooking Oil (5 mg /mL Soluble in isopropanol)

Triglycerides in cooking oil

ChromCore Polar columns are based on amide-embedded C18 modified silica particles with exhaustive end-capping for excellent aqueous compatibility and different selectivity. They are designed for applications requiring highly aqueous mobile phase and/or selectivity different from C18.

ChromCore AR C18 columns are based on bonding a unique sterically protected salina to the siloxane bond without using acid labile endcapping reagents. They feature exceptional chemical stability under acidic condition and excellent aqueous compatibility, as well as selectivity complimentary to ChromCore C18 columns.

ChromCoreTM Polar C18

ChromCoreTM AR C18

ChromCore C8 columns are based on high surface coverage C8 modified silica particles with exhaustive end-capping to minimize undesired silanol activity. They are designed for separating analytes with intermediate to high hydrophobici-ty.

ChromCoreTM C8

0 10 20 30 40Time [min]

Peanut Oil

Sesame Oil

Olive Oil

Applications

ChromCore AQ C8 columns are based on proprietary C8 modified silica particles for excellent aqueous compatibility. They are designed for separating analytes with intermediate to high hydrophobicity under highly aqueous mobile phase conditions.

ChromCoreTM AQ C8

Flow rate:Temperature:Injection:Detection:Sample: Peaks:

ChromCoreTMAQ C18, 5 μm4.6×250mmA) H20B) MeOHt (min) A B -10 28 72 0 28 72 5.7 28 72 8.0 20 80 15.0 20 80 20.0 28 721 mL/minRoom temperature5 μLUV 290 nmSidenafil Mixed standard solution1. Aminotadalafil 2. N-Demethylsildenafil3. Sildenafil Citrate4. Homo Sildenafil 5. Demethylthiophane6. Thiohamoxdenafil

Sildenafil in urine sample

ChromCore AQ C18 columns are based on proprietary C18 modified silica particles for excellent aqueous compatibility. They are the column of choice for applications requiring highly aqueous mobile phase and/or C18 selectivity.

ChromCoreTM AQ C18

Column Black: ChromCoreTM AQ C18，5µm Column Blue: Agilent TC-C18，5µm Dimension: 4.6×250mmMobile phase: 70/30 v/v H2O/MeCNFlow rate: 1 mL/minTemperature: Room temperatureInjection: 25 μLDetection: UV 220 nmSample: 5 ppm Glycyl-L-Histidyl-L-Lysine

Glycyl-L-Histidyl-L-Lysine

ChromCore Agilent

Applications

ChromCore C4 columns are based on high surface coverage C4 modified silica particles with exhaustive end-capping. They are designed for separating analytes of high hydrophobicity.

ChromCoreTM C4

Retention Time

Peak area

Peak Height

Symmetry

Theoretical plates

7.867min

12.427

7.707min

10.364

0 9 13.5 18Time [min]

8ppm Standard

4ppm Standard in sample

0 9 12 15Time [min]

ChromCore AQ C18

Column: Dimension:Mobile phase:

Gradient:

Flow rate:Injection:Temperature:Detection:Sample:

ChromCoreTM120 C8 5 µm4.6×150 mmA）MeCNB）IPAC）0.1 M Acetic acid, pH 5.0t (min) A B C-10 90 5 50 90 5 530 0 95 540 0 95 51.0 mL/min5 µL30oCCADCooking Oil (5 mg /mL Soluble in isopropanol)

Triglycerides in cooking oil

ChromCore Polar columns are based on amide-embedded C18 modified silica particles with exhaustive end-capping for excellent aqueous compatibility and different selectivity. They are designed for applications requiring highly aqueous mobile phase and/or selectivity different from C18.

ChromCore AR C18 columns are based on bonding a unique sterically protected salina to the siloxane bond without using acid labile endcapping reagents. They feature exceptional chemical stability under acidic condition and excellent aqueous compatibility, as well as selectivity complimentary to ChromCore C18 columns.

ChromCoreTM Polar C18

ChromCoreTM AR C18

ChromCore C8 columns are based on high surface coverage C8 modified silica particles with exhaustive end-capping to minimize undesired silanol activity. They are designed for separating analytes with intermediate to high hydrophobici-ty.

ChromCoreTM C8

0 10 20 30 40Time [min]

Peanut Oil

Sesame Oil

Olive Oil

Applications

ChromCore AQ C8 columns are based on proprietary C8 modified silica particles for excellent aqueous compatibility. They are designed for separating analytes with intermediate to high hydrophobicity under highly aqueous mobile phase conditions.

ChromCoreTM AQ C8

ChromCore C30 columns are based on C30 alkyl chain modified silica particles with exhaustive end-capping for excellent shape selectivity. They are designed as the column of choice for structural isomer separations, such as lipids and fat soluble vitamins. In addition, they can be used in highly aqueous conditions, making it useful for separating hydrophilic compounds as well.

ChromCoreTM C30

Column: ChromCoreTM C30, 5 μmDimension: 4.6×250 mmMobile phase: A）H2O B）MeCNGradient: t (min) A B 0 40 60 20 40 60 170 25 75 180 10 90 190 10 90Flow rate: 1.0 mL/minTemperature: 20 ℃Injection: 5 μLDetection: UV 205 nmSamples: 1-4. Teprenone Isomer 5-6. Teprenone

Teprenone and Teprenone Isomer

100 120 140 180

Time [min]

Applications

ChromCore Phenyl columns are based on high surface coverage propylbenzene modified silica particles with exhaus-tive end-capping for stronger retention of aromatic analytes. They are designed for a broad range of applications that requiring selectivity towards aromatic analytes, such as isomers of aromatic and heterocyclic pharmaceuticals and catechins.

ChromCore Biphenyl columns are based on high surface coverage biphenyl modified silica particles with exhaustive end-capping to for unique selectivity for aromatic analytes. They are designed for applications requiring different selectivity towards aromatic analytes, comparing to ChromCore Phenyl and ChromCore PFP columns.

ChromCoreTM Phenyl

ChromCoreTM Biphenyl

ChromCore PFP columns are based on pentafluorobenzene (PFP) modified silica particles with exhaustive end-cap-ping. They are designed for a broad range of applications that require selectivity different from ChromCore Phenyl for aromatic analytes, such as aromatic halogenated compounds and vitamin E isomers.

ChromCoreTM PFP

Column: ChromCoreTMPFP, 5 µmDimension: 4.6×150 mmMobile phase: 65/35 v/v MeOH/H2O, with 0.05% FA (v%) and 0.07% Ammonium formate(wt%) Flow rate: 1 mL/minTemperature: 30 ºCInjection: 5 μLDetection: UV 254 nmSample: 1. 25-OH Vitamin D3, 10 µg /mL 2. 3-epi-25-OH Vitamin D3, 10 µg /mL 3. 25-OH Vitamin D2, 5 µg /mL 4. 3-epi-25-OH Vitamin D2, 10 µg /mL

Column: ChromCorTMPFP 5 µm Dimension: 4.6 × 150 mmMobile phase: v/v 35/65 MeOH/H2OFlow rate: 1 mL/minTemperature: 30 ºCInjection: 5 μLDetection: UV 240 nmSample: 1. Hydrocortisone 2. Prednisolone 3. Prednisone

Vitamin D metabolites Steroid

20 45 70 95 120Time [min]

10 20 30 50

Time [min]60

Applications

ChromCore C30 columns are based on C30 alkyl chain modified silica particles with exhaustive end-capping for excellent shape selectivity. They are designed as the column of choice for structural isomer separations, such as lipids and fat soluble vitamins. In addition, they can be used in highly aqueous conditions, making it useful for separating hydrophilic compounds as well.

ChromCoreTM C30

Column: ChromCoreTM C30, 5 μmDimension: 4.6×250 mmMobile phase: A）H2O B）MeCNGradient: t (min) A B 0 40 60 20 40 60 170 25 75 180 10 90 190 10 90Flow rate: 1.0 mL/minTemperature: 20 ℃Injection: 5 μLDetection: UV 205 nmSamples: 1-4. Teprenone Isomer 5-6. Teprenone

Teprenone and Teprenone Isomer

100 120 140 180

Time [min]

Applications

ChromCore Phenyl columns are based on high surface coverage propylbenzene modified silica particles with exhaus-tive end-capping for stronger retention of aromatic analytes. They are designed for a broad range of applications that requiring selectivity towards aromatic analytes, such as isomers of aromatic and heterocyclic pharmaceuticals and catechins.

ChromCore Biphenyl columns are based on high surface coverage biphenyl modified silica particles with exhaustive end-capping to for unique selectivity for aromatic analytes. They are designed for applications requiring different selectivity towards aromatic analytes, comparing to ChromCore Phenyl and ChromCore PFP columns.

ChromCoreTM Phenyl

ChromCoreTM Biphenyl

ChromCore PFP columns are based on pentafluorobenzene (PFP) modified silica particles with exhaustive end-cap-ping. They are designed for a broad range of applications that require selectivity different from ChromCore Phenyl for aromatic analytes, such as aromatic halogenated compounds and vitamin E isomers.

ChromCoreTM PFP

Column: ChromCoreTMPFP, 5 µmDimension: 4.6×150 mmMobile phase: 65/35 v/v MeOH/H2O, with 0.05% FA (v%) and 0.07% Ammonium formate(wt%) Flow rate: 1 mL/minTemperature: 30 ºCInjection: 5 μLDetection: UV 254 nmSample: 1. 25-OH Vitamin D3, 10 µg /mL 2. 3-epi-25-OH Vitamin D3, 10 µg /mL 3. 25-OH Vitamin D2, 5 µg /mL 4. 3-epi-25-OH Vitamin D2, 10 µg /mL

Column: ChromCorTMPFP 5 µm Dimension: 4.6 × 150 mmMobile phase: v/v 35/65 MeOH/H2OFlow rate: 1 mL/minTemperature: 30 ºCInjection: 5 μLDetection: UV 240 nmSample: 1. Hydrocortisone 2. Prednisolone 3. Prednisone

Vitamin D metabolites Steroid

20 45 70 95 120Time [min]

10 20 30 50

Time [min]60

Applications

Ordering Information

ChromCoreTM Reversed-Phase LC Columns 5μm

Column 4.6×250mm 4.6×150mm 4.6×100mm 2.1×150mm 2.1×100mmC18

120 C18

300 C18

AQ C18

Polar C18

AR C18

120 C8

300 C8

300 C4

300 C4-T

Phenyl

A001-050018-04625S

A001-050012-04625S

A001-050030-04625S

A201-050018-04625S

A060-050012-04625S

A401-050012-04625S

A007-050018-04625S

A007-050012-04625S

A007-050030-04625S

A207-050018-04625S

A062-050018-04625S

A026-050012-04625S

A026-050030-04625S

A226-050030-04625S

A011-050012-04625S

A043-050012-04625S

A001-050018-04615S

A001-050012-04615S

A001-050030-04615S

A201-050018-04615S

A060-050012-04615S

A401-050012-04615S

A007-050018-04615S

A007-050012-04615S

A007-050030-04615S

A207-050018-04615S

A062-050018-04615S

A026-050012-04615S

A026-050030-04615S

A226-050030-04615S

A011-050012-04615S

A043-050012-04615S

A001-050018-04610S

A001-050012-04610S

A001-050030-04610S

A201-050018-04610S

A060-050012-04610S

A401-050012-04610S

A007-050018-04610S

A007-050012-04610S

A007-050030-04610S

A207-050018-04610S

A062-050018-04610S

A026-050012-04610S

A026-050030-04610S

A226-050030-04610S

A011-050012-04610S

A043-050012-04610S

A001-050018-02115S

A001-050012-02115S

A001-050030-02115S

A201-050018-02115S

A060-050012-02115S

A401-050012-02115S

A007-050018-02115S

A007-050012-02115S

A007-050030-02115S

A207-050018-02115S

A062-050018-02115S

A026-050012-02115S

A026-050030-02115S

A226-050030-02115S

A011-050012-02115S

A043-050012-02115S

A001-050018-02110S

A001-050012-02110S

A001-050030-02110S

A201-050018-02110S

A060-050012-02110S

A401-050012-02110S

A007-050018-02110S

A007-050012-02110S

A007-050030-02110S

A207-050018-02110S

A062-050018-02110S

A026-050012-02110S

A026-050030-02110S

A226-050030-02110S

A011-050012-02110S

A043-050012-02110S

120 C18

300 C18

AQ C18

Polar C18

AR C18

120 C8

300 C8

300 C4

300 C4-T

Phenyl

A001-030018-04625S

A001-030012-04625S

A001-030030-04625S

A201-030018-04625S

A060-030012-04625S

A401-030012-04625S

A007-030018-04625S

A007-030012-04625S

A007-030030-04625S

A207-030018-04625S

A062-030018-04625S

A026-030012-04625S

A026-030030-04625S

A226-030030-04625S

A011-030012-04625S

A043-030012-04625S

A001-030018-04615S

A001-030012-04615S

A001-030030-04615S

A201-030018-04615S

A060-030012-04615S

A401-030012-04615S

A007-030018-04615S

A007-030012-04615S

A007-030030-04615S

A207-030018-04615S

A062-030018-04615S

A026-030012-04615S

A026-030030-04615S

A226-030030-04615S

A011-030012-04615S

A043-030012-04615S

A001-030018-04610S

A001-030012-04610S

A001-030030-04610S

A201-030018-04610S

A060-030012-04610S

A401-030012-04610S

A007-030018-04610S

A007-030012-04610S

A007-030030-04610S

A207-030018-04610S

A062-030018-04610S

A026-030012-04610S

A026-030030-04610S

A226-030030-04610S

A011-030012-04610S

A043-030012-04610S

A001-030018-02115S

A001-030012-02115S

A001-030030-02115S

A201-030018-02115S

A060-030012-02115S

A401-030012-02115S

A007-030018-02115S

A007-030012-02115S

A007-030030-02115S

A207-030018-02115S

A062-030018-02115S

A026-030012-02115S

A026-030030-02115S

A226-030030-02115S

A011-030012-02115S

A043-030012-02115S

A001-030018-02110S

A001-030012-02110S

A001-030030-02110S

A201-030018-02110S

A060-030012-02110S

A401-030012-02110S

A007-030018-02110S

A007-030012-02110S

A007-030030-02110S

A207-030018-02110S

A062-030018-02110S

A026-030012-02110S

A026-030030-02110S

A226-030030-02110S

A011-030012-02110S

A043-030012-02110S

ChromCoreTM Normal Phase/HILIC LC ColumnsChromCore Normal Phase/HILIC columns are designed to complement ChromCore reversed-phase columns for separating polar and hydrophilic compounds.

Monodispered, high-purity, porous, spherical particles for high efficiency, mass transfer and mechanical

strength

Versatile column chemistries for broad selectivity coverage

Excellent chromatography performance for symmetrical peaks

Good column to column consistency

Main features

Column :Dimension :Mobile phase :Flow rate :Temperature :Injection :Detection :Sample

ChromCoreTMSilica, 5 μm4.6x250 mm97/3 v/v C6H14( Hexane)/ CH3COOCH2CH32 mL/min30 ℃20 μLUV 275 nm1. Coenzyme Q10 Isomer 2. Coenzyme Q10

1. Coenzyme Q10

ChromCore Silica columns are based on unmodified silica particles with tightly controlled surface. They are designed for separating hydrophilic analytes, such as steroids under normal phase condition.

ChromCoreTM Silica

0 3 6 9 12 15

Time[min]

Applications

120 C18

300 C18

AQ C18

Polar C18

AR C18

120 C8

300 C8

300 C4

300 C4-T

Phenyl

A001-050018-04625S

A001-050012-04625S

A001-050030-04625S

A201-050018-04625S

A060-050012-04625S

A401-050012-04625S

A007-050018-04625S

A007-050012-04625S

A007-050030-04625S

A207-050018-04625S

A062-050018-04625S

A026-050012-04625S

A026-050030-04625S

A226-050030-04625S

A011-050012-04625S

A043-050012-04625S

A001-050018-04615S

A001-050012-04615S

A001-050030-04615S

A201-050018-04615S

A060-050012-04615S

A401-050012-04615S

A007-050018-04615S

A007-050012-04615S

A007-050030-04615S

A207-050018-04615S

A062-050018-04615S

A026-050012-04615S

A026-050030-04615S

A226-050030-04615S

A011-050012-04615S

A043-050012-04615S

A001-050018-04610S

A001-050012-04610S

A001-050030-04610S

A201-050018-04610S

A060-050012-04610S

A401-050012-04610S

A007-050018-04610S

A007-050012-04610S

A007-050030-04610S

A207-050018-04610S

A062-050018-04610S

A026-050012-04610S

A026-050030-04610S

A226-050030-04610S

A011-050012-04610S

A043-050012-04610S

A001-050018-02115S

A001-050012-02115S

A001-050030-02115S

A201-050018-02115S

A060-050012-02115S

A401-050012-02115S

A007-050018-02115S

A007-050012-02115S

A007-050030-02115S

A207-050018-02115S

A062-050018-02115S

A026-050012-02115S

A026-050030-02115S

A226-050030-02115S

A011-050012-02115S

A043-050012-02115S

A001-050018-02110S

A001-050012-02110S

A001-050030-02110S

A201-050018-02110S

A060-050012-02110S

A401-050012-02110S

A007-050018-02110S

A007-050012-02110S

A007-050030-02110S

A207-050018-02110S

A062-050018-02110S

A026-050012-02110S

A026-050030-02110S

A226-050030-02110S

A011-050012-02110S

A043-050012-02110S

120 C18

300 C18

AQ C18

Polar C18

AR C18

120 C8

300 C8

300 C4

300 C4-T

Phenyl

A001-030018-04625S

A001-030012-04625S

A001-030030-04625S

A201-030018-04625S

A060-030012-04625S

A401-030012-04625S

A007-030018-04625S

A007-030012-04625S

A007-030030-04625S

A207-030018-04625S

A062-030018-04625S

A026-030012-04625S

A026-030030-04625S

A226-030030-04625S

A011-030012-04625S

A043-030012-04625S

A001-030018-04615S

A001-030012-04615S

A001-030030-04615S

A201-030018-04615S

A060-030012-04615S

A401-030012-04615S

A007-030018-04615S

A007-030012-04615S

A007-030030-04615S

A207-030018-04615S

A062-030018-04615S

A026-030012-04615S

A026-030030-04615S

A226-030030-04615S

A011-030012-04615S

A043-030012-04615S

A001-030018-04610S

A001-030012-04610S

A001-030030-04610S

A201-030018-04610S

A060-030012-04610S

A401-030012-04610S

A007-030018-04610S

A007-030012-04610S

A007-030030-04610S

A207-030018-04610S

A062-030018-04610S

A026-030012-04610S

A026-030030-04610S

A226-030030-04610S

A011-030012-04610S

A043-030012-04610S

A001-030018-02115S

A001-030012-02115S

A001-030030-02115S

A201-030018-02115S

A060-030012-02115S

A401-030012-02115S

A007-030018-02115S

A007-030012-02115S

A007-030030-02115S

A207-030018-02115S

A062-030018-02115S

A026-030012-02115S

A026-030030-02115S

A226-030030-02115S

A011-030012-02115S

A043-030012-02115S

A001-030018-02110S

A001-030012-02110S

A001-030030-02110S

A201-030018-02110S

A060-030012-02110S

A401-030012-02110S

A007-030018-02110S

A007-030012-02110S

A007-030030-02110S

A207-030018-02110S

A062-030018-02110S

A026-030012-02110S

A026-030030-02110S

A226-030030-02110S

A011-030012-02110S

A043-030012-02110S

ChromCoreTM Normal Phase/HILIC LC ColumnsChromCore Normal Phase/HILIC columns are designed to complement ChromCore reversed-phase columns for separating polar and hydrophilic compounds.

Monodispered, high-purity, porous, spherical particles for high efficiency, mass transfer and mechanical

strength

Versatile column chemistries for broad selectivity coverage

Excellent chromatography performance for symmetrical peaks

Good column to column consistency

Main features

ChromCoreTMSilica, 5 μm4.6x250 mm97/3 v/v C6H14( Hexane)/ CH3COOCH2CH32 mL/min30 ℃20 μLUV 275 nm1. Coenzyme Q10 Isomer 2. Coenzyme Q10

1. Coenzyme Q10

ChromCore Silica columns are based on unmodified silica particles with tightly controlled surface. They are designed for separating hydrophilic analytes, such as steroids under normal phase condition.

ChromCoreTM Silica

0 3 6 9 12 15

Time[min]

Applications

ChromCore NH2, 5 μm4.6x250 mm70/30 v/v MeCN / H2O1 mL/min40 ℃20 μLRID1. Fructose2. Glucose 3. Sucrose 4. Maltose 5. Lactose

Carbohydrate Silica NH2 HILIC-Diol CN HILIC-Imidazole HILIC-Amide

ChromCore NH2 columns are based on high surface coverage propyl amino silica particles. They are designed for separating hydrophilic analytes, such as monosaccharides, oligosaccharides, and sugar alcohols under HILIC condi-tions.

ChromCore HILIC-Diol columns are based on proprietary diol modified silica particles. They have a neutral surface with intermediate polarity, serving as an alternative to silica column for separation of pesticides, herbicides, pharmaceutical metabolites, polar natural products.

ChromCore CN columns are based on high surface coverage cyan modified silica particles. They are designed for separating polar analytes, such as penicillins and steroids.

ChromCoreTM Sugar columns are made of monodispersed PS/DVB spherical particles，through a unique sulfonation bonding process to form H+, Na+ , or Ca+ forms, for the analysis requirements of different types of sugar,sugar alcohols and organic acids.

ChromCoreTM NH2

ChromCoreTM HILIC-Diol

ChromCoreTM Sugar ColumnsChromCoreTM CN

ChromCoreTM Normal Phase/HILIC LC Columns 3μm

ChromCoreTMNormal Phase/HILIC LC Columns 5μm

ChromCore HILIC-Imidazole columns are based on proprietary polar imidazole modified silica particles. With a polar and N-containing ring structure, these columns feature a weak anion exchange property with intermediate polarity, providing selectivity complementary to other HILIC columns for separating highly polar analytes in a broad range of applications.

ChromCore HILIC-Amide columns are based on proprietary amido modified silica particles. They have higher polarity than HILIC-Diol columns, and serve as an alternative for separating highly polar analytes in a broad range of applica-tions.

ChromCoreTM HILIC-Imidazole

ChromCoreTM HILIC-Amide

Silica NH2 HILIC-Diol CN HILIC-Imidazole HILIC-Amide

A003-030012-04625S

A003-030012-04615S

A008-030012-04625S

A008-030012-04615S

A020-030012-04625S

A020-030012-04615S

A010-030012-04625S

A010-030012-04615S

A208-030012-04625S

A208-030012-04615S

A068-030012-04625S

A068-030012-04615S

4.6×250mm

4.6×150mm

4.6×250mm

4.6×150mm

A003-050012-04625S

A003-050012-04615S

A008-050012-04625S

A008-050012-04615S

A020-050012-04625S

A020-050012-04615S

A010-050012-04625S

A010-050012-04615S

A208-050012-04625S

A208-050012-04615S

A068-050012-04625S

A068-050012-04615S

Applications

4 6 8 12 14

Time [min]

Functional group

Base Substrate

Particle Size

Cross-linking

pH Range

Temperature Range

Pressure Range

Application

6 & 8 µm

< 95 °C

1200 psi

organic acids and alcohols

Sugar-10H Sugar-10Ca Sugar-10Na

Monodispersed high purity PS/DVB spherical particles

-SO3Ca

6 & 8 µm

< 95 °C

1200 psi

Mono-& di saccharides

-SO3Na

6 & 8 µm

< 95 °C

1200 psi

sugar alcohols

Parameters

ChromCore NH2, 5 μm4.6x250 mm70/30 v/v MeCN / H2O1 mL/min40 ℃20 μLRID1. Fructose2. Glucose 3. Sucrose 4. Maltose 5. Lactose

Carbohydrate Silica NH2 HILIC-Diol CN HILIC-Imidazole HILIC-Amide

ChromCore NH2 columns are based on high surface coverage propyl amino silica particles. They are designed for separating hydrophilic analytes, such as monosaccharides, oligosaccharides, and sugar alcohols under HILIC condi-tions.

ChromCore HILIC-Diol columns are based on proprietary diol modified silica particles. They have a neutral surface with intermediate polarity, serving as an alternative to silica column for separation of pesticides, herbicides, pharmaceutical metabolites, polar natural products.

ChromCore CN columns are based on high surface coverage cyan modified silica particles. They are designed for separating polar analytes, such as penicillins and steroids.

ChromCoreTM Sugar columns are made of monodispersed PS/DVB spherical particles，through a unique sulfonation bonding process to form H+, Na+ , or Ca+ forms, for the analysis requirements of different types of sugar,sugar alcohols and organic acids.

ChromCoreTM NH2

ChromCoreTM HILIC-Diol

ChromCoreTM Sugar ColumnsChromCoreTM CN

ChromCoreTM Normal Phase/HILIC LC Columns 3μm

ChromCoreTMNormal Phase/HILIC LC Columns 5μm

ChromCore HILIC-Imidazole columns are based on proprietary polar imidazole modified silica particles. With a polar and N-containing ring structure, these columns feature a weak anion exchange property with intermediate polarity, providing selectivity complementary to other HILIC columns for separating highly polar analytes in a broad range of applications.

ChromCore HILIC-Amide columns are based on proprietary amido modified silica particles. They have higher polarity than HILIC-Diol columns, and serve as an alternative for separating highly polar analytes in a broad range of applica-tions.

ChromCoreTM HILIC-Imidazole

ChromCoreTM HILIC-Amide

Silica NH2 HILIC-Diol CN HILIC-Imidazole HILIC-Amide

A003-030012-04625S

A003-030012-04615S

A008-030012-04625S

A008-030012-04615S

A020-030012-04625S

A020-030012-04615S

A010-030012-04625S

A010-030012-04615S

A208-030012-04625S

A208-030012-04615S

A068-030012-04625S

A068-030012-04615S

4.6×250mm

4.6×150mm

4.6×250mm

4.6×150mm

A003-050012-04625S

A003-050012-04615S

A008-050012-04625S

A008-050012-04615S

A020-050012-04625S

A020-050012-04615S

A010-050012-04625S

A010-050012-04615S

A208-050012-04625S

A208-050012-04615S

A068-050012-04625S

A068-050012-04615S

Applications

4 6 8 12 14

Time [min]

Functional group

Base Substrate

Particle Size

Cross-linking

pH Range

Temperature Range

Pressure Range

Application

6 & 8 µm

< 95 °C

1200 psi

organic acids and alcohols

Sugar-10H Sugar-10Ca Sugar-10Na

Monodispersed high purity PS/DVB spherical particles

-SO3Ca

6 & 8 µm

< 95 °C

1200 psi

Mono-& di saccharides

-SO3Na

6 & 8 µm

< 95 °C

1200 psi

sugar alcohols

Parameters

ChromCore Sugar-10Ca, 6 μm7.8x300 mmH2O0.5 mL/min80 ℃5 μLRID1. Sucrose 2 . Galactose 3 . Fructose 4 . Mannitol 5 . Sorbitol

Column:Dimension:Mobile Phase:Flow rate: Temperature:Detection:

ChromCore Sugar-10H, 8 μm7.8x300 mmH2SO4 in H2O, pH2.50.5 mL/min30 ℃UV207 nm

Column: Dimension: Mobile Phase: Flow rate:Temperature: Injection: Detection: Samples:

ChromCore Sugar-10H, 6 μm7.8x300 mm9mM H2SO40.5 mL/min65 ℃5 μLRID1. Citcric Acid 2. Malic Acid 3. Succinic Acid 4. Formic Acid 5. Acetic Acid

Ribayirin Mannitol

Organic Acids

Order Information

ChromCore Sugar-10H ChromCore Sugar-10Na ChromCore Sugar-10CaColumn Tubing

4.6x250mm

7.8x300mm

A017-060010-04625S

A017-060010-07830S

A017-080010-04625S

A017-080010-07830S

A058-080010-04625S

A058-080010-07830S

A019-080010-04625S

A019-080010-07830S

A058-060010-04625S

A058-060010-07830S

A019-060010-04625S

A019-060010-07830S

4.6x250mm

7.8x300mm

Particle Size

0 7.5 15 22.5 30Time [min]

Applications

NanoChrom provides preparative columns and semi-preparative columns,and customized columns.

ChromCore C18

ChromCore 120 C18

ChromCore C8

ChromCore 120 C8

ChromCore Silica

ChromCore NH2

A001-050018-21225S

A001-050012-21225S

A007-050018-21225S

A007-050012-21225S

A003-050012-21225S

A008-050012-21225S

A001-050018-21215S

A001-050012-21215S

A007-050018-21215S

A007-050012-21215S

A003-050012-21215S

A008-050012-21215S

A001-050018-10025S

A001-050012-10025S

A007-050018-10025S

A007-050012-10025S

A003-050012-10025S

A008-050012-10025S

A001-050018-10015S

A001-050012-10015S

A007-050018-10015S

A007-050012-10015S

A003-050012-10015S

A008-050012-10015S

21.2×250mm 21.2×150mm 10×250mm 10×150mm

0 5 10 15 20Time [min]

ChromCoreTM Preparative Columns

ChromCore Sugar-10Ca, 6 μm7.8x300 mmH2O0.5 mL/min80 ℃5 μLRID1. Sucrose 2 . Galactose 3 . Fructose 4 . Mannitol 5 . Sorbitol

Column:Dimension:Mobile Phase:Flow rate: Temperature:Detection:

ChromCore Sugar-10H, 8 μm7.8x300 mmH2SO4 in H2O, pH2.50.5 mL/min30 ℃UV207 nm

Column: Dimension: Mobile Phase: Flow rate:Temperature: Injection: Detection: Samples:

ChromCore Sugar-10H, 6 μm7.8x300 mm9mM H2SO40.5 mL/min65 ℃5 μLRID1. Citcric Acid 2. Malic Acid 3. Succinic Acid 4. Formic Acid 5. Acetic Acid

Ribayirin Mannitol

Organic Acids

Order Information

ChromCore Sugar-10H ChromCore Sugar-10Na ChromCore Sugar-10CaColumn Tubing

4.6x250mm

7.8x300mm

A017-060010-04625S

A017-060010-07830S

A017-080010-04625S

A017-080010-07830S

A058-080010-04625S

A058-080010-07830S

A019-080010-04625S

A019-080010-07830S

A058-060010-04625S

A058-060010-07830S

A019-060010-04625S

A019-060010-07830S

4.6x250mm

7.8x300mm

Particle Size

0 7.5 15 22.5 30Time [min]

Applications

NanoChrom provides preparative columns and semi-preparative columns,and customized columns.

ChromCore C18

ChromCore 120 C18

ChromCore C8

ChromCore 120 C8

ChromCore Silica

ChromCore NH2

A001-050018-21225S

A001-050012-21225S

A007-050018-21225S

A007-050012-21225S

A003-050012-21225S

A008-050012-21225S

A001-050018-21215S

A001-050012-21215S

A007-050018-21215S

A007-050012-21215S

A003-050012-21215S

A008-050012-21215S

A001-050018-10025S

A001-050012-10025S

A007-050018-10025S

A007-050012-10025S

A003-050012-10025S

A008-050012-10025S

A001-050018-10015S

A001-050012-10015S

A007-050018-10015S

A007-050012-10015S

A003-050012-10015S

A008-050012-10015S

21.2×250mm 21.2×150mm 10×250mm 10×150mm

0 5 10 15 20Time [min]

ChromCoreTM Preparative Columns

EcoPakTM C18EcoPak C18 columns are based on monodispersed, high-purity, spherical, porous silica particles, combined with advanced C18 column chemistry and a well-developed, cost-effective production process. They feature high efficiency, good column to column reproducibility and satisfactory chromatography performance, suitable for a broad range of cost-sensitive applications such as Chinese medicines, chemical, food & beverage, etc.

Parameters

Bonded Phase

Particle Size

Pore Diameter

Carbon Load

Surface Area

End-capped

pH Range

EcoPak 120 C18 5μm, 4.6×250mm

EcoPak 120 C18 5μm, 4.6×150mm

E001-050012-04625S

E001-050012-04615S

120 Å

300 m2/g

Single End-capping

2 - 10

4.6×150mm , 4.6×250mm

Column Part No.

EcoPakTM HPLC Columns

BioCoreTM HPLC Columns

BioCoreTM Protein A --------------------------------------------------------------------- 24 BioCoreTM SEC ---------------------------------------------- 25

EcoPakTM C18EcoPak C18 columns are based on monodispersed, high-purity, spherical, porous silica particles, combined with advanced C18 column chemistry and a well-developed, cost-effective production process. They feature high efficiency, good column to column reproducibility and satisfactory chromatography performance, suitable for a broad range of cost-sensitive applications such as Chinese medicines, chemical, food & beverage, etc.

Parameters

Bonded Phase

Particle Size

Pore Diameter

Carbon Load

Surface Area

End-capped

pH Range

EcoPak 120 C18 5μm, 4.6×250mm

EcoPak 120 C18 5μm, 4.6×150mm

E001-050012-04625S

E001-050012-04615S

120 Å

300 m2/g

Single End-capping

2 - 10

4.6×150mm , 4.6×250mm

Column Part No.

EcoPakTM HPLC Columns

BioCoreTM HPLC Columns

BioCoreTM Protein A --------------------------------------------------------------------- 24 BioCoreTM SEC ---------------------------------------------- 25

BioCore Protein A is based on monodispersed, wide-pore, PS/DVB spherical particles, combined with advanced surface modification technology using alkaline-resistant recombinant protein A. It is designed for fast titer analysis of monoclo-nal antibodies (mAb) and Fc fusion proteins.

Main Features

BioCoreTM Protein A

Unique bonding of alkaline-resistant Protein A for low ligand leaching

High physical strength for better column lifetime

High efficiency for fast analysis

High binding capacity and low carryover

Good linear dynamic range

01 2423

Product Name

Functional Group

Substrate

Particle Size

Pore Size

Maximum Linear Velocity

Linear Range

Dynamic Binding Capacity

Maximum Pressure

Operating Temperature

pH Range

Chemical Stability

Storage

Product Information

BioCore Protein A

pH stable rProtein A

PS/DVB

15 μm

1000 Å

1.39 cm/s

0-200 μg

>20 mg/mL (RT 3.3 min)

1450 psi

< 40 ℃

0.5 M NaOH

20% Ethanol（2 ~ 8 ℃）

Protein A Calibration Curve

Column:Dimension: Mobile phase A:Mobile phase B:Gradient:

Flow rate: Temperature:Injection: Detection: Sample:

BioCore Protein A, 15 μm 2.1×30 mm 150 mM NaCl in 50 mM PBS (pH 7.0)150 mM NaCl in 50 mM PBS (pH 2.5) Time (min) %A %B 0 100 0 0.5 100 0 0.51 0 100 1.5 0 100 1.51 100 0 2.5 100 0 2.0 mL/min 30 ℃ 20 μL UV 280 nm IgG, 0-6.25 mg/mL

Titer Analysis in HCC

0 1.0 1.5 2.0 2.5Time (min)

Wash Elute Re-Equilibrate

Column: Dimension: Mobile phase A:Mobile phase B:Gradient: Flow rate: Temperature: Injection: Detection: Sample:

BioCore Protein A, 15 μm 2.1×30 mm 150 mM NaCl in 50 mM PBS (pH 7.0)150 mM NaCl in 50 mM PBS (pH 2.5) Time (min) %A %B 0 100 0 0.5 100 0 0.51 0 100 1.5 0 100 1.51 100 0 2.5 100 0 2.0 mL/min 30 ℃ 20 μL UV 280 nm IgG (2.4 mg/mL)

R2=0.9989

Protein A Standard Curves for lgG E

BioCore Protein A is based on monodispersed, wide-pore, PS/DVB spherical particles, combined with advanced surface modification technology using alkaline-resistant recombinant protein A. It is designed for fast titer analysis of monoclo-nal antibodies (mAb) and Fc fusion proteins.

Main Features

BioCoreTM Protein A

Unique bonding of alkaline-resistant Protein A for low ligand leaching

High efficiency for fast analysis

High binding capacity and low carryover

Good linear dynamic range

01 2423

Product Name

Functional Group

Substrate

Particle Size

Pore Size

Maximum Linear Velocity

Linear Range

Dynamic Binding Capacity

Maximum Pressure

Operating Temperature

pH Range

Chemical Stability

Storage

Product Information

BioCore Protein A

pH stable rProtein A

PS/DVB

15 μm

1000 Å

1.39 cm/s

0-200 μg

>20 mg/mL (RT 3.3 min)

1450 psi

< 40 ℃

0.5 M NaOH

20% Ethanol（2 ~ 8 ℃）

Protein A Calibration Curve

Column:Dimension: Mobile phase A:Mobile phase B:Gradient:

Flow rate: Temperature:Injection: Detection: Sample:

BioCore Protein A, 15 μm 2.1×30 mm 150 mM NaCl in 50 mM PBS (pH 7.0)150 mM NaCl in 50 mM PBS (pH 2.5) Time (min) %A %B 0 100 0 0.5 100 0 0.51 0 100 1.5 0 100 1.51 100 0 2.5 100 0 2.0 mL/min 30 ℃ 20 μL UV 280 nm IgG, 0-6.25 mg/mL

Titer Analysis in HCC

0 1.0 1.5 2.0 2.5Time (min)

Wash Elute Re-Equilibrate

Column: Dimension: Mobile phase A:Mobile phase B:Gradient: Flow rate: Temperature: Injection: Detection: Sample:

BioCore Protein A, 15 μm 2.1×30 mm 150 mM NaCl in 50 mM PBS (pH 7.0)150 mM NaCl in 50 mM PBS (pH 2.5) Time (min) %A %B 0 100 0 0.5 100 0 0.51 0 100 1.5 0 100 1.51 100 0 2.5 100 0 2.0 mL/min 30 ℃ 20 μL UV 280 nm IgG (2.4 mg/mL)

R2=0.9989

Protein A Standard Curves for lgG E

BioCore SEC - a family of high performance size exclusion chromatography (SEC) columns for separating a broad range of biomolecules based on size

BioCore SEC is a family of high performance, size exclusion chromatography (SEC) columns for separating a broad range of biomolecules based on the size of analyte. The column technology involves creation of a neutral hydro-philic layer on the surface of specially designed high-strength, monodispered, silica particles followed by well-es-tablished production process. Therefore, BioCore SEC columns have abroad application range in pharmaceutical, biopharmaceutical and academia research.

Size Exclusion Chromatography (SEC) is the dominant separation mode for polymers. In SEC, components of a mixture are separated based on the size (dynamic volume) using a column packed with porous particles. Large molecules cannot or can only partially penetrate the pores, whereas smaller molecules can access all or a larger number of pores. Therefore, large molecules elute from the column first followed by smaller molecules, and the smallest molecules that can access all the pores elute last from the column.

BioCoreTM SEC

BioCore SEC-150 - designed for separating peptides, glycans, small oligos, small proteins.

BioCore SEC-300 - designed for mAb aggregate determination.

BioCore SEC-500 - designed for separating larger proteins and oligos.

High column efficiency, high resolution

Minimal undesired interactions between stationary phase and analytes, resulting on good peak shape and

recovery

Broad range of applications, including small molecule drugs, peptides, proteins, oligos, glycans, etc.

The features

Parameters

Calibration Curve

PEGGlucanGlobular Protein

Functional Group

Base Substrate

Particle Size

Pore Diameter

pH Range

Temperature Range

Pressure Range

Calibration Curve （PEG）

Calibration Curve（Glucan）

Calibration Curve（globular protein）

Monodispersed high purity silica

150 Å

2 ‒ 8

< 40 °C

6000 psi

200-15，000

1,000 ‒ 50,000

5,000 ‒ 150,000

300 Å

2 ‒ 8

< 40 °C

6000 psi

1,000 ‒ 100,000

5,000 ‒ 150,000

10,000 ‒ 1,000,000

500 Å

2 ‒ 8

< 40 °C

6000 psi

5,000 ‒ 200,000

20,000 ‒ 500,000

20,000 ‒ 2,000,000

Peptides, Glycans, Small oligos, Small proteins

Application mAb aggregate determination

Large proteins and DNA/RNA

BioCore SEC-150 BioCore SEC-300 BioCore SEC-500

SEC silica particlesLarge moleculesSmaller molecules

4 6 8 10 12

BioCore SEC150BioCore SEC300BioCore SEC500

Time/min

4 9 14

Time/min Time/min

BioCore SEC - a family of high performance size exclusion chromatography (SEC) columns for separating a broad range of biomolecules based on size

BioCore SEC is a family of high performance, size exclusion chromatography (SEC) columns for separating a broad range of biomolecules based on the size of analyte. The column technology involves creation of a neutral hydro-philic layer on the surface of specially designed high-strength, monodispered, silica particles followed by well-es-tablished production process. Therefore, BioCore SEC columns have abroad application range in pharmaceutical, biopharmaceutical and academia research.

Size Exclusion Chromatography (SEC) is the dominant separation mode for polymers. In SEC, components of a mixture are separated based on the size (dynamic volume) using a column packed with porous particles. Large molecules cannot or can only partially penetrate the pores, whereas smaller molecules can access all or a larger number of pores. Therefore, large molecules elute from the column first followed by smaller molecules, and the smallest molecules that can access all the pores elute last from the column.

BioCoreTM SEC

BioCore SEC-150 - designed for separating peptides, glycans, small oligos, small proteins.

BioCore SEC-300 - designed for mAb aggregate determination.

BioCore SEC-500 - designed for separating larger proteins and oligos.

High column efficiency, high resolution

Minimal undesired interactions between stationary phase and analytes, resulting on good peak shape and

recovery

Broad range of applications, including small molecule drugs, peptides, proteins, oligos, glycans, etc.

The features

Parameters

Calibration Curve

PEGGlucanGlobular Protein

Functional Group

Base Substrate

Particle Size

Pore Diameter

pH Range

Temperature Range

Pressure Range

Calibration Curve （PEG）

Calibration Curve（Glucan）

Calibration Curve（globular protein）

150 Å

2 ‒ 8

< 40 °C

6000 psi

200-15，000

1,000 ‒ 50,000

5,000 ‒ 150,000

300 Å

2 ‒ 8

< 40 °C

6000 psi

1,000 ‒ 100,000

5,000 ‒ 150,000

10,000 ‒ 1,000,000

500 Å

2 ‒ 8

< 40 °C

6000 psi

5,000 ‒ 200,000

20,000 ‒ 500,000

20,000 ‒ 2,000,000

Peptides, Glycans, Small oligos, Small proteins

Application mAb aggregate determination

Large proteins and DNA/RNA

BioCore SEC-150 BioCore SEC-300 BioCore SEC-500

SEC silica particlesLarge moleculesSmaller molecules

4 6 8 10 12

Time/min

4 9 14

Time/min Time/min

Column Black: Column Blue: Column Red: Dimension: Mobile phase: Flow rate: Temperature: Injection: Detection: Peaks:

BioCore SEC-150, 5 μm7.8×300 mm100 mM Ammonium acetate0.5 mL/min35 ℃25 μLRID (35℃)Low molecular weight heparin was used to calibrate CRS

（10mg /mL）

Column: BioCore SEC-300, 5 μmDimension: 4.6×300 mmMobile phase: 150 mM Phosphate Buffered Saline (pH 6.8)Flow rate: 0.35 mL/minTemperature: 30 ℃Injection: 5 μLDetection: UV 280 nmSample: IgG1（2.6 mg /mL in water）Peaks: 1. IgG1 2. IgG1 Aggregate

BioCore SEC-500, 5 μm4.6×300 mm150mM Phosphate Buffered Saline (pH 6.8)0.35 mL/min30 ℃5 μLUV 280 nmFusion Protein（1 mg /mL in water）

Applications

Protein Separation

Cefdiazine

Ceftriaxone

Low-Molecular-Weight Haprin (EP 9.8)

Monoclonal antibody (IgG1)

Fusion Protein

0 6 9 12 15Time [min]

SEC-500

SEC-300

SEC-15012 3

Column: Dimension: Mobile phase: Flow rate: Temperature: Injection: Detection: Sample: Peaks:

0 6 9 12 15

0 12 18 24 30

Time [min]

0 6 9 12 15Time [min]

0 6 9 12 15

Time [min]

Column: BioCore SEC-150, 5 μmDimensin: 7.8×300 mmMobile phase: Phosphate Buffered Saline(pH 7.0)Flow rate: 1 mL/minTemperature: 30 ℃Injection: 2 μLDetection: UV 231 nmSample: Ceftriaxone sodium in aqueous solutionPeaks: 1. Ceftriaxone 2-5. Ceftriaxone Polymers

BioCore SEC-150, 5 μmBioCore SEC-300, 5 μmBioCore SEC-500, 5 μm4.6×300 mm150 mM Phosphate Buffered Saline (pH 6.8)0.35 mL/min30 ℃5 μLUV 280 nm1. Thyroglobulin（0.5 mg /mL）- 669,000 Da2. Conalbumin（1 mg /mL）- 75,000 Da3. Ribonuclease A（1 mg /mL）- 13,700 Da4. Uracil（0.1 mg /mL) ‒ 112 Da

BioCore SEC-150, 5 μm7.8×300 mm95/5 v/v Phosphate Buffered Saline (pH 7.0)/ Acetonitrile1 mL/min30 ℃20 μLUV 231 nmCefodizine Sodium（0.5 mg /mL in water）1. Cefodizine2-5. Cefodizine Polymers

Column: Dimension: Mobile phase: Flow rate: Temperature: Injection: Detection: Sample:

Column Black: Column Blue: Column Red: Dimension: Mobile phase: Flow rate: Temperature: Injection: Detection: Peaks:

BioCore SEC-150, 5 μm7.8×300 mm100 mM Ammonium acetate0.5 mL/min35 ℃25 μLRID (35℃)Low molecular weight heparin was used to calibrate CRS

（10mg /mL）

Column: BioCore SEC-300, 5 μmDimension: 4.6×300 mmMobile phase: 150 mM Phosphate Buffered Saline (pH 6.8)Flow rate: 0.35 mL/minTemperature: 30 ℃Injection: 5 μLDetection: UV 280 nmSample: IgG1（2.6 mg /mL in water）Peaks: 1. IgG1 2. IgG1 Aggregate

BioCore SEC-500, 5 μm4.6×300 mm150mM Phosphate Buffered Saline (pH 6.8)0.35 mL/min30 ℃5 μLUV 280 nmFusion Protein（1 mg /mL in water）

Applications

Protein Separation

Cefdiazine

Ceftriaxone

Low-Molecular-Weight Haprin (EP 9.8)

Monoclonal antibody (IgG1)

Fusion Protein

0 6 9 12 15Time [min]

SEC-500

SEC-300

SEC-15012 3

0 6 9 12 15

0 12 18 24 30

Time [min]

0 6 9 12 15Time [min]

0 6 9 12 15

Time [min]

Column: BioCore SEC-150, 5 μmDimensin: 7.8×300 mmMobile phase: Phosphate Buffered Saline(pH 7.0)Flow rate: 1 mL/minTemperature: 30 ℃Injection: 2 μLDetection: UV 231 nmSample: Ceftriaxone sodium in aqueous solutionPeaks: 1. Ceftriaxone 2-5. Ceftriaxone Polymers

BioCore SEC-150, 5 μmBioCore SEC-300, 5 μmBioCore SEC-500, 5 μm4.6×300 mm150 mM Phosphate Buffered Saline (pH 6.8)0.35 mL/min30 ℃5 μLUV 280 nm1. Thyroglobulin（0.5 mg /mL）- 669,000 Da2. Conalbumin（1 mg /mL）- 75,000 Da3. Ribonuclease A（1 mg /mL）- 13,700 Da4. Uracil（0.1 mg /mL) ‒ 112 Da

BioCore SEC-150, 5 μm7.8×300 mm95/5 v/v Phosphate Buffered Saline (pH 7.0)/ Acetonitrile1 mL/min30 ℃20 μLUV 231 nmCefodizine Sodium（0.5 mg /mL in water）1. Cefodizine2-5. Cefodizine Polymers

Column: Dimension: Mobile phase: Flow rate: Temperature: Injection: Detection: Sample:

* Used as a guard column

** Need to be used with Guard Holder（ P/N ： Guard-04601D-C1）

UniChiral® Columns Monoclonal antibody (IgG4)

Human serum albumin and its polymers

0 6 9 12 15

Time [min]

0 15 22.5 30Time [min]

BioCore SEC-500, 5 μm4.6×300 mm150 mM Phosphate Buffered Saline (pH6.8)0.35 mL/min30 ℃5 μLUV 280nmIgG4（16 mg /mL in water）1. IgG42-6. IgG4 Aggregates

BioCore SEC-300, 5 μm7.8×300 mm + 7.8×300 mm in series150 mM Phosphate Buffered Saline (pH6.8)1 mL/min30 ℃10 μLUV 210 nmHuman albumin solution1. Human albumin2. Human albumin dimer3. Human albumin polymer

BioCore SEC-150

BioCore SEC-300

BioCore SEC-500

B213-050015-07830S

B213-050030-07830S

B213-050050-07830S

B213-050015-04630S

B213-050030-04630S

B213-050050-04630S

B213-050015-04605S

B213-050030-04605S

B213-050050-04605S

B213-050015-04601S

B213-050030-04601S

B213-050050-04601S

5µm 7.8x300mm 5µm 4.6x300mm 5µm 4.6x50mm *

UniChiral® Chiral HPLC Columns ------------------------------------------------------------------- 31

Guard Cartridge **

* Used as a guard column

** Need to be used with Guard Holder（ P/N ： Guard-04601D-C1）

UniChiral® Columns Monoclonal antibody (IgG4)

Human serum albumin and its polymers

0 6 9 12 15

Time [min]

0 15 22.5 30Time [min]

BioCore SEC-500, 5 μm4.6×300 mm150 mM Phosphate Buffered Saline (pH6.8)0.35 mL/min30 ℃5 μLUV 280nmIgG4（16 mg /mL in water）1. IgG42-6. IgG4 Aggregates

BioCore SEC-300, 5 μm7.8×300 mm + 7.8×300 mm in series150 mM Phosphate Buffered Saline (pH6.8)1 mL/min30 ℃10 μLUV 210 nmHuman albumin solution1. Human albumin2. Human albumin dimer3. Human albumin polymer

BioCore SEC-150

BioCore SEC-300

BioCore SEC-500

B213-050015-07830S

B213-050030-07830S

B213-050050-07830S

B213-050015-04630S

B213-050030-04630S

B213-050050-04630S

B213-050015-04605S

B213-050030-04605S

B213-050050-04605S

B213-050015-04601S

B213-050030-04601S

B213-050050-04601S

5µm 7.8x300mm 5µm 4.6x300mm 5µm 4.6x50mm *

UniChiral® Chiral HPLC Columns ------------------------------------------------------------------- 31

Guard Cartridge **

UniChiral® is a family of polysaccharide chiral chromatography media based on NanoMicro's unique silica matrix with monodispersed particle size and large pore morphology (Figure 1). This product line includes OD, OJ, OZ, AS, and AD that employs Cellulose and Amylose as surface coating for the derivatization of varied chiral selector phases (Table 1). They provide a variety of complementary selectivity that allow for screen most effective chiral separation and purifica-tion under Reversed Phase, Polar Organic, Normal Phase, and SFC conditions. While the 5 µm and 10 µm are routine catalogue offering, other particle sizes can be customized as required. Besides UniChiral® bulk media, NanoMicro also offers their HPLC columns for analytical and semi-preparative purification applications. UniChiral® products are very affordable and highly competitive in quality and performance. They will make your chiral separation experience easy and productive.

UniChiral® Chromatography Media and Columns for High Performance Chiral Separation

Performance Demonstration

UniChiral OD 5µm4.6x250 mmHexane / IPA=9:11mL/min25 ℃UV 254 nmTrans-Stilbene oxide

UniChiral®AD 5µm4.6x250 mmEtOH1 mL/minUV 270 nm25 ℃Furoin

UniChiral®AD 5µm 4.6x250mm Competitor D AD-H

UniChiral OD 5µm 4.6x250 mmHexane / IPA=9:11 mL/min25 ℃UV 254 nm2,2,2-Trifliuoro-1-(9-anthryl)ethanol

5um UniChiral®HPLC Columns

AD AS OD OJ OZ4.6×250mm

4.6×150mm

CAAD-050100-04625S

CAAD-050100-04615S

CAAS-050100-04625S

CAAS-050100-04615S

CAOD-050100-04625S

CAOD-050100-04615S

CAOJ-050100-04625S

CAOJ-050100-04615S

CAOZ-050100-04625S

CAOZ-050100-04615S

Figure 1. Representative SEM images of 5 µm UniChiral® silica matrix. Note their uniformed particle size and large pore (1000 Å) morphology. Table 1. Characteristics of UniChiral® bulk media

Highly competitive enantioselectivity and column efficiencyUnique mono-sized silica matrix provides superior product lifetime and low back pressure.A family of chiral selectors provides a variety of complementary selectivityIn-house silica matrix production, polysaccharide coating, chiral phase functionalization, and column packing provides us complete control of our product quality and traceability.Very affordable prices and highly competitive in quality and performance

Highlights

Product

UniChiral® OD

UniChiral® OJ

UniChiral® OZ

UniChiral® AS

UniChiral® AD

Surface Functional Groups

5 μm 10 μm

Particle Size

5 μm 10 μm

UniChiral®ODCompertitor C OD-H

Column:Dimension: Mobile phase :Flow rate: Temperature:Detection: Sample:

UniChiral® is a family of polysaccharide chiral chromatography media based on NanoMicro's unique silica matrix with monodispersed particle size and large pore morphology (Figure 1). This product line includes OD, OJ, OZ, AS, and AD that employs Cellulose and Amylose as surface coating for the derivatization of varied chiral selector phases (Table 1). They provide a variety of complementary selectivity that allow for screen most effective chiral separation and purifica-tion under Reversed Phase, Polar Organic, Normal Phase, and SFC conditions. While the 5 µm and 10 µm are routine catalogue offering, other particle sizes can be customized as required. Besides UniChiral® bulk media, NanoMicro also offers their HPLC columns for analytical and semi-preparative purification applications. UniChiral® products are very affordable and highly competitive in quality and performance. They will make your chiral separation experience easy and productive.

UniChiral® Chromatography Media and Columns for High Performance Chiral Separation

Performance Demonstration

UniChiral OD 5µm4.6x250 mmHexane / IPA=9:11mL/min25 ℃UV 254 nmTrans-Stilbene oxide

UniChiral®AD 5µm4.6x250 mmEtOH1 mL/minUV 270 nm25 ℃Furoin

UniChiral®AD 5µm 4.6x250mm Competitor D AD-H

UniChiral OD 5µm 4.6x250 mmHexane / IPA=9:11 mL/min25 ℃UV 254 nm2,2,2-Trifliuoro-1-(9-anthryl)ethanol

5um UniChiral®HPLC Columns

AD AS OD OJ OZ4.6×250mm

4.6×150mm

CAAD-050100-04625S

CAAD-050100-04615S

CAAS-050100-04625S

CAAS-050100-04615S

CAOD-050100-04625S

CAOD-050100-04615S

CAOJ-050100-04625S

CAOJ-050100-04615S

CAOZ-050100-04625S

CAOZ-050100-04615S

Figure 1. Representative SEM images of 5 µm UniChiral® silica matrix. Note their uniformed particle size and large pore (1000 Å) morphology. Table 1. Characteristics of UniChiral® bulk media

Highly competitive enantioselectivity and column efficiencyUnique mono-sized silica matrix provides superior product lifetime and low back pressure.A family of chiral selectors provides a variety of complementary selectivityIn-house silica matrix production, polysaccharide coating, chiral phase functionalization, and column packing provides us complete control of our product quality and traceability.Very affordable prices and highly competitive in quality and performance

Highlights

Product

UniChiral® OD

UniChiral® OJ

UniChiral® OZ

UniChiral® AS

UniChiral® AD

Surface Functional Groups

5 μm 10 μm

Particle Size

5 μm 10 μm

UniChiral®ODCompertitor C OD-H

Column:Dimension: Mobile phase :Flow rate: Temperature:Detection: Sample:

PVP based SPE

SelectCoreTM

HLBSelectCoreTM

MCXSelectCoreTM

MAXSelectCoreTM

WCXSelectCoreTM

Sample preparation is an essential part of chromatographic and spectroscopic analyses. The aim of sample preparation is to provide relatively free of interferences, prolong lifetime of the column or instrument and be compatible with the intended analytical method.

Polyvinyl pyrrolidone based SPE is a highly competitive solid-phase extraction product introduced by NanoChrom. It has good water immersion and chemical stability, the surface has both hydrophilic and hydrophobic function group, so as to have a balanced adsorption effect on various types polar and non-polar compounds.

Sample Pretreatment

SelectCoreTM SPE columns

1. PVP based SPENew Generation Monodispersed Hydrophilic Lipophilic Balanced SPE

a. Good retention of polar compoundsb. High recovery of basic compoundsc. Better reproductive results even if cartridge running dryd. Wider pH tolerance range between pH1-14

Silica Based SPE ------------------------------------------------------------------------------------ 38

PVP based SPE (HLB) --------------------------------------------------------- 34 Inorganic Adsorbent ----------------------------------------------------------------------------- 39

Multilayer SPE --------------------------------------------------------------------------------------- 40PS/DVB Based SPE ------------------------------------------------------------------------- 37

Lipophilic

Hydrophilic

PVP based SPE cartridges can be widely used in the pretreatment of veterinary drug residues in food, such as tetracy-cline, chloramphenicol, nitrofuran, quinolone etc. They also can be used for the extraction and purification of biologi-cal samples such as plasma and urine.

PVP based SPE

SelectCoreTM

HLBSelectCoreTM

MCXSelectCoreTM

MAXSelectCoreTM

WCXSelectCoreTM

Sample preparation is an essential part of chromatographic and spectroscopic analyses. The aim of sample preparation is to provide relatively free of interferences, prolong lifetime of the column or instrument and be compatible with the intended analytical method.

Polyvinyl pyrrolidone based SPE is a highly competitive solid-phase extraction product introduced by NanoChrom. It has good water immersion and chemical stability, the surface has both hydrophilic and hydrophobic function group, so as to have a balanced adsorption effect on various types polar and non-polar compounds.

Sample Pretreatment

1. PVP based SPENew Generation Monodispersed Hydrophilic Lipophilic Balanced SPE

a. Good retention of polar compoundsb. High recovery of basic compoundsc. Better reproductive results even if cartridge running dryd. Wider pH tolerance range between pH1-14

Silica Based SPE ------------------------------------------------------------------------------------ 38

PVP based SPE (HLB) --------------------------------------------------------- 34 Inorganic Adsorbent ----------------------------------------------------------------------------- 39

Multilayer SPE --------------------------------------------------------------------------------------- 40PS/DVB Based SPE ------------------------------------------------------------------------- 37

Lipophilic

Hydrophilic

PVP based SPE cartridges can be widely used in the pretreatment of veterinary drug residues in food, such as tetracy-cline, chloramphenicol, nitrofuran, quinolone etc. They also can be used for the extraction and purification of biologi-cal samples such as plasma and urine.

MAX WCX

Comared with Brand A and Brand B, SelectCore HLB could provide higher dynamic binding capacity, closed with Brand W

Compared with Brand A and Brand W, SelectCore HLB has faster elution velocity

SelectCoreTM PVP based SPE

Compared with other brand HLB，SelectCore HLB has less elution volume

Features

1. High dynamic binding capacity 2. Fast elution velocity

3. Less elution volume

Oxytetracycline

Chlortetracycline Doxycycline

Tetracycline

Size & Package HLB MCX WAX

30 mg/1mL 100/pkg

60mg/3mL 50/pkg

150mg/6mL 30/pkg

200mg/6mL 30/pkg

500mg/6mL 30/pkg

HLB060-010030-1

HLB060-030060-1

HLB060-060150-1

HLB060-060200-1

HLB060-060500-1

MCX060-010030-1

MCX060-030060-1

MCX060-060150-1

MCX060-060200-1

MCX060-060500-1

WCX060-010030-1

WCX060-030060-1

WCX060-060150-1

WCX060-060200-1

WCX060-060500-1

MAX060-010030-1

MAX060-030060-1

MAX060-060150-1

MAX060-060200-1

MAX060-060500-1

WAX060-010030-1

WAX060-030060-1

WAX060-060150-1

WAX060-060200-1

WAX060-060500-1

0 5 10 15 20 25 30 35 40 45 50

Elution Volume (mL)

SelectCore HLB Brand W HLB Brand A HLB Brand B HLB

0 1 2 3 4 5 6 7 8 9 10 11 120

SelectCore HLB Brand A HLB Brand B HLB Brand W HLB

Elution Step0 1 2 3 4 5 6 7 8 9 10 11 12

Elution Step

0 1 2 3 4 5 6 7 8 9 10 11 12-10

Elution step

0 1 2 3 4 5 6 7 8 9 10 11 120

Elution Step

HLB Brand A Brand W0

SPE column

30 drops 10 drops

样品

前处

理产

Mechanism

Hydrophilic-Lipophilic Balance

Strong cation exchange

Strong anion exchange

Weak cation exchange

Weak anion exchange

Features and Application

SelectCore HLB

SelectCore MCX

SelectCore MAX

SelectCore WCX

SelectCore WAX

Good water immersion and chemical stability, the surface has both hydrophilic and hydrophobic function group, so as to have a balanced adsorption effect on all kinds of polar and non-polar compoundsIts sample capacity is much higher than ODS (3-10 times)

monodisperse polystyrene-divinylbenzene resin particles grafted with aromatic sulfonic acid groups. This polymeric mixed-mode sorbent features reversed-phase and strong cation exchange retention mechanisms, allowing for superb retention for basic compounds.

monodisperse polystyrene-divinylbenzene resin particles grafted with aromatic quaternary ammonium groups. This polymeric mixed-mode sorbent features reversed-phase and strong anion exchange retention mechanisms, allowing for superb retention for acidic compounds.

monodisperse microporous polystyrene-divinylbenzene resin particles grafted with carboxyl groups. This polymeric mixed-mode sorbent features reversed-phase and weak cation exchange retention mechanisms, allowing for superb retention for strong bases such as quaternary ammonium cations.

monodisperse microporous polystyrene-divinylbenzene resin particles grafted with amine functional groups. This polymeric mixed-mode sorbent features reversed-phase and weak anion exchange retention mechanisms, allowing for superb retention for strong acids.

MAX WCX

Comared with Brand A and Brand B, SelectCore HLB could provide higher dynamic binding capacity, closed with Brand W

Compared with Brand A and Brand W, SelectCore HLB has faster elution velocity

SelectCoreTM PVP based SPE

Compared with other brand HLB，SelectCore HLB has less elution volume

Features

1. High dynamic binding capacity 2. Fast elution velocity

3. Less elution volume

Oxytetracycline

Chlortetracycline Doxycycline

Tetracycline

Size & Package HLB MCX WAX

30 mg/1mL 100/pkg

60mg/3mL 50/pkg

150mg/6mL 30/pkg

200mg/6mL 30/pkg

500mg/6mL 30/pkg

HLB060-010030-1

HLB060-030060-1

HLB060-060150-1

HLB060-060200-1

HLB060-060500-1

MCX060-010030-1

MCX060-030060-1

MCX060-060150-1

MCX060-060200-1

MCX060-060500-1

WCX060-010030-1

WCX060-030060-1

WCX060-060150-1

WCX060-060200-1

WCX060-060500-1

MAX060-010030-1

MAX060-030060-1

MAX060-060150-1

MAX060-060200-1

MAX060-060500-1

WAX060-010030-1

WAX060-030060-1

WAX060-060150-1

WAX060-060200-1

WAX060-060500-1

0 5 10 15 20 25 30 35 40 45 50

Elution Volume (mL)

SelectCore HLB Brand W HLB Brand A HLB Brand B HLB

0 1 2 3 4 5 6 7 8 9 10 11 120

Elution Step0 1 2 3 4 5 6 7 8 9 10 11 12

Elution Step

0 1 2 3 4 5 6 7 8 9 10 11 12-10

Elution step

0 1 2 3 4 5 6 7 8 9 10 11 120

Elution Step

HLB Brand A Brand W0

SPE column

30 drops 10 drops

样品

前处

理产

Mechanism

Hydrophilic-Lipophilic Balance

Strong cation exchange

Strong anion exchange

Weak cation exchange

Weak anion exchange

Features and Application

SelectCore HLB

SelectCore MCX

SelectCore MAX

SelectCore WCX

SelectCore WAX

Good water immersion and chemical stability, the surface has both hydrophilic and hydrophobic function group, so as to have a balanced adsorption effect on all kinds of polar and non-polar compoundsIts sample capacity is much higher than ODS (3-10 times)

monodisperse polystyrene-divinylbenzene resin particles grafted with aromatic sulfonic acid groups. This polymeric mixed-mode sorbent features reversed-phase and strong cation exchange retention mechanisms, allowing for superb retention for basic compounds.

monodisperse polystyrene-divinylbenzene resin particles grafted with aromatic quaternary ammonium groups. This polymeric mixed-mode sorbent features reversed-phase and strong anion exchange retention mechanisms, allowing for superb retention for acidic compounds.

monodisperse microporous polystyrene-divinylbenzene resin particles grafted with carboxyl groups. This polymeric mixed-mode sorbent features reversed-phase and weak cation exchange retention mechanisms, allowing for superb retention for strong bases such as quaternary ammonium cations.

monodisperse microporous polystyrene-divinylbenzene resin particles grafted with amine functional groups. This polymeric mixed-mode sorbent features reversed-phase and weak anion exchange retention mechanisms, allowing for superb retention for strong acids.

2. PS/DVB based SPE 3.Silica based SPEA highly cross-linked polystyrene-divinyl benzene copolymer sorbent that allows for rapid adsorption and separation of hydrophobic substances, such as phenol, surfactants, non-B pyridine bromide, antibiotics, amino acids and peptides, etc.Ideal for the extraction of polar analytes that are not adequately retained on a C18 or C8 sorbent. The nonselective characteristic of this sorbent is useful for screening applications where a broad range of analytes is to be extracted.

AmorphousSpherical

订货信息

Can be used as an alternative to octadecyl-bonded silica for preparation of analytes that weakly adsorb to silica-based reversed phase sorbents. Compatible with sample or eluents at high and low pH.

Narrow particle size distribution and excellent resolution.Can be used as an alternative to octadecyl-bonded silica for preparation of analytes that weakly adsorb to silica-based reversed phase sorbents.

Narrow particle size distribution and excellent resolution

PS/DVB 100μmSelectCore PSL

Martrix Particle Features and Applications

SelectCore PSS

SelectCore P-SCX

SelectCore X3

42μm Narrow particle size distribution and excellent resolution with GPC mode

Product Part Number

SelectCore PSL 250mg/3mL; 50/pkg

SelectCore PSS 60mg/3mL; 50/pkg

PSL100-03250-1

PSL100-06250-1

PSL100-06500-1

PSS040-03060-1

PSS040-06150-1

PSS040-06200-1

PSS040-06500-1

Features and applicationsParticle size

50 μmSelectCore Silica

SelectCore C18

SelectCore NH2

SelectCore PSA

50 μm

Polar sorbent, used primarily to adsorb analytes from non-polar solvents like hydrocar-bons, chloro- or fluoro-subsitituted hydrocarbons or less polar esters and ethers; elution with more polar solvents like polar esters, ethers, alcohols, acetonitrile or water; the binding mechanism can be hydrogen bonding or dipole-dipole interaction; silica can also be used in aqueous medium as a cation exchanger of intermediate strength, or as a support for liquid-liquid partition separations with a polar stationary phase.

Silica based bonded phase, used to adsorb analytes of even weak hydrophobicity from aqueous solutions; typical applications include drugs and their metabolites in serum, plasma or urine, desalting of peptides, trace organics in environmental water samples, organic acids in beverages;

Silica based bonded phase with weakly basic surface; can be used as a polar sorbent, like silica, with different selectivity for acidic/basic analytes or as weak anion exchanger in aqueous medium below pH 8; applications include phenols and phenolic pigments, petroleum fractionation, sacharides,drugs and drug metabolites.

Silica based phase with ethylenediamine-N-propyl that contains both primary and secondary amines; A weak anion exchanger with a pKa of 10.1 and 10.9; Similar to aminopropyl SPE phases (NH2) in terms of selectivity, but has a much higher capacity due to presence of secondary amine; Strong affinity and high capacity for removing fatty acids, organic acids, and some polar pigments and sugars when conducting multi-resi-due pesticide analysis in foods

Size & Package Silica C18 NH2 PSA

100mg/1ml 100/pkg SI050-010100-1

SI050-030200-1

SI050-030500-1

SI050-060500-1

SI050-061000-1

C18050-010100-1

C18050-030200-1

C18050-030500-1

C18050-060500-1

C18050-061000-1

NH050-010100-1

NH050-030200-1

NH050-030500-1

NH050-060500-1

NH050-061000-1

PSA050-010100-1

PSA050-030200-1

PSA050-030500-1

PSA050-060500-1

PSA050-061000-1

200mg/3ml 50/pkg

500mg/3ml 50/pkg

500mg/6ml 30/pkg

1000mg/6ml 30/pkg

PS/DVB

2. PS/DVB based SPE 3.Silica based SPEA highly cross-linked polystyrene-divinyl benzene copolymer sorbent that allows for rapid adsorption and separation of hydrophobic substances, such as phenol, surfactants, non-B pyridine bromide, antibiotics, amino acids and peptides, etc.Ideal for the extraction of polar analytes that are not adequately retained on a C18 or C8 sorbent. The nonselective characteristic of this sorbent is useful for screening applications where a broad range of analytes is to be extracted.

AmorphousSpherical

订货信息

Can be used as an alternative to octadecyl-bonded silica for preparation of analytes that weakly adsorb to silica-based reversed phase sorbents. Compatible with sample or eluents at high and low pH.

Narrow particle size distribution and excellent resolution.Can be used as an alternative to octadecyl-bonded silica for preparation of analytes that weakly adsorb to silica-based reversed phase sorbents.

Narrow particle size distribution and excellent resolution

PS/DVB 100μmSelectCore PSL

Martrix Particle Features and Applications

SelectCore PSS

SelectCore P-SCX

SelectCore X3

42μm Narrow particle size distribution and excellent resolution with GPC mode

Product Part Number

PSL100-03250-1

PSL100-06250-1

PSL100-06500-1

PSS040-03060-1

PSS040-06150-1

PSS040-06200-1

PSS040-06500-1

Features and applicationsParticle size

50 μmSelectCore Silica

SelectCore C18

SelectCore NH2

SelectCore PSA

50 μm

Polar sorbent, used primarily to adsorb analytes from non-polar solvents like hydrocar-bons, chloro- or fluoro-subsitituted hydrocarbons or less polar esters and ethers; elution with more polar solvents like polar esters, ethers, alcohols, acetonitrile or water; the binding mechanism can be hydrogen bonding or dipole-dipole interaction; silica can also be used in aqueous medium as a cation exchanger of intermediate strength, or as a support for liquid-liquid partition separations with a polar stationary phase.

Silica based bonded phase, used to adsorb analytes of even weak hydrophobicity from aqueous solutions; typical applications include drugs and their metabolites in serum, plasma or urine, desalting of peptides, trace organics in environmental water samples, organic acids in beverages;

Silica based bonded phase with weakly basic surface; can be used as a polar sorbent, like silica, with different selectivity for acidic/basic analytes or as weak anion exchanger in aqueous medium below pH 8; applications include phenols and phenolic pigments, petroleum fractionation, sacharides,drugs and drug metabolites.

Silica based phase with ethylenediamine-N-propyl that contains both primary and secondary amines; A weak anion exchanger with a pKa of 10.1 and 10.9; Similar to aminopropyl SPE phases (NH2) in terms of selectivity, but has a much higher capacity due to presence of secondary amine; Strong affinity and high capacity for removing fatty acids, organic acids, and some polar pigments and sugars when conducting multi-resi-due pesticide analysis in foods

Size & Package Silica C18 NH2 PSA

100mg/1ml 100/pkg SI050-010100-1

SI050-030200-1

SI050-030500-1

SI050-060500-1

SI050-061000-1

C18050-010100-1

C18050-030200-1

C18050-030500-1

C18050-060500-1

C18050-061000-1

NH050-010100-1

NH050-030200-1

NH050-030500-1

NH050-060500-1

NH050-061000-1

PSA050-010100-1

PSA050-030200-1

PSA050-030500-1

PSA050-060500-1

PSA050-061000-1

200mg/3ml 50/pkg

500mg/3ml 50/pkg

500mg/6ml 30/pkg

1000mg/6ml 30/pkg

PS/DVB

5. Multilayer SPE4. Inorganic Adsorbent

SelectCoreTM Alumina

SelectCoreTM Florisil

Similar in use to silica; available in acidic[A], neutral[N], and basic[B] grades; Highly active, polar surface;Alumina also exhibits specific interactions with the pi-electrons of aromatic hydrocarbons, making it useful for appications like crude oil fractionation; Acidic and basic grades can also be used as low-capacity ion-exchangers, which, unlike polymer-based exchang-ers, are unaffected by high energy, radioactive materials.

Highly active, polar sorbent with a slightly basic surface for adsorption of low to moderate polarity species from nonaqueous solutions; Specifically designed for adsorption of pesticides using official AOAC and EPA methods; Other applications include polychlorinated biphenyls in tranformer oil

SelectCoreTM GCBSelectCore GCB is composed of sheet-like, nonporous graphitized carbon black with aromatic six-member ring structure and positive charges. It has reversed phase and ion exchange retention mechanisms, retaining non-polar compounds, such as organochlorine pesticides, as well as polar compounds, such as surfactants.

SelectCoreTM PAPolyamide sorbent, separation mechanism mainly based on hydrogen bonds; Recommended application: separation of phenolic compounds (e.g., isolation of natural products), carboxylic acids, aromatic nitro compounds

SelectCoreTM GCB/NH2

SelectCore GCB/NH2 combines the merits of both GCB and NH2 sorbents and is able to remove interfering compo-sitions such as pigments, sterols and fatty acids in food samples, making it an effective packing for cleaning samples up in multiresidual pesticide analysis.

Florisil Size & Package

100mg/1ml100/pkg

FL060-010100-1 ALA060-010100-1

ALA060-030200-1

ALA060-030500-1

ALA060-060500-1

ALA060-061000-1

ALB060-010100-1

ALB060-030200-1

ALB060-030500-1

ALB060-060500-1

ALB060-061000-1

ALN060-010100-1

ALN060-030200-1

ALN060-030500-1

ALN060-060500-1

ALN060-061000-1

GCB100-010100-1

GCB100-030200-1

GCB100-030500-1

GCB100-060500-1 PA100-060500-1

PA100-061000-1GCB100-061000-1

FL060-030200-1

FL060-030500-1

FL060-061000-1

200mg/3ml50/pkg

500mg/3ml50/pkg

500mg/6ml30/pkg

1000mg/6ml30/pkg

Alumina A Alumina N Alumina N GCB PA

SelectCore GCB/PSA is a sorbent similar to GCB/NH2 and suitable for cleaning samples up in multiresidues pesticide analysis. Due to its additional secondary amino groups, PSA has higher ion exchange capability and ability to chelate some metal ions, thus providing GCB/PSA with unique selectivity different from GCB/NH2.

Product Part Number

SelectCore GCB/NH2 500mg/500mg/6mL; 30/pkg

SelectCore GCB/PSA 500mg/500mg/6mL; 30/pkg

GN100-060500-1

GP100-060500-1

SelectCoreTM GCB/PSA

5. Multilayer SPE4. Inorganic Adsorbent

SelectCoreTM Alumina

SelectCoreTM Florisil

Similar in use to silica; available in acidic[A], neutral[N], and basic[B] grades; Highly active, polar surface;Alumina also exhibits specific interactions with the pi-electrons of aromatic hydrocarbons, making it useful for appications like crude oil fractionation; Acidic and basic grades can also be used as low-capacity ion-exchangers, which, unlike polymer-based exchang-ers, are unaffected by high energy, radioactive materials.

Highly active, polar sorbent with a slightly basic surface for adsorption of low to moderate polarity species from nonaqueous solutions; Specifically designed for adsorption of pesticides using official AOAC and EPA methods; Other applications include polychlorinated biphenyls in tranformer oil

SelectCoreTM GCBSelectCore GCB is composed of sheet-like, nonporous graphitized carbon black with aromatic six-member ring structure and positive charges. It has reversed phase and ion exchange retention mechanisms, retaining non-polar compounds, such as organochlorine pesticides, as well as polar compounds, such as surfactants.

SelectCoreTM PAPolyamide sorbent, separation mechanism mainly based on hydrogen bonds; Recommended application: separation of phenolic compounds (e.g., isolation of natural products), carboxylic acids, aromatic nitro compounds

SelectCoreTM GCB/NH2

SelectCore GCB/NH2 combines the merits of both GCB and NH2 sorbents and is able to remove interfering compo-sitions such as pigments, sterols and fatty acids in food samples, making it an effective packing for cleaning samples up in multiresidual pesticide analysis.

Florisil Size & Package

100mg/1ml100/pkg

FL060-010100-1 ALA060-010100-1

ALA060-030200-1

ALA060-030500-1

ALA060-060500-1

ALA060-061000-1

ALB060-010100-1

ALB060-030200-1

ALB060-030500-1

ALB060-060500-1

ALB060-061000-1

ALN060-010100-1

ALN060-030200-1

ALN060-030500-1

ALN060-060500-1

ALN060-061000-1

GCB100-010100-1

GCB100-030200-1

GCB100-030500-1

GCB100-060500-1 PA100-060500-1

PA100-061000-1GCB100-061000-1

FL060-030200-1

FL060-030500-1

FL060-061000-1

200mg/3ml50/pkg

500mg/3ml50/pkg

500mg/6ml30/pkg

1000mg/6ml30/pkg

Alumina A Alumina N Alumina N GCB PA

SelectCore GCB/PSA is a sorbent similar to GCB/NH2 and suitable for cleaning samples up in multiresidues pesticide analysis. Due to its additional secondary amino groups, PSA has higher ion exchange capability and ability to chelate some metal ions, thus providing GCB/PSA with unique selectivity different from GCB/NH2.

Product Part Number

SelectCore GCB/NH2 500mg/500mg/6mL; 30/pkg

SelectCore GCB/PSA 500mg/500mg/6mL; 30/pkg

GN100-060500-1

GP100-060500-1

SelectCoreTM GCB/PSA

NanoChrom BP Column product comprises of polysiloxane phases, polyethylene glycol phases, porous-layer-open tubular (PLOT) phases, low column bleed or MS (Mass Spec) grade columns across all stationary phases, and custom-made columns. NanoChrom BP Column is developed by an American company which own proprietary column technologies, manufacturing process, and high standard quality control. Each NanoChrom BP column is carefully prepared and signature made for quality and performance excellence to deliver to our customers worldwide.

NanoChrom BP stationary phases to yield similar or identical chromatographic performances to brand ones. NanoChrom BP columns have virtually minimal impact transitioning from another column brand.

NanoChrom BP-5MS （red）vs H-5MS （black） NanoChrom BP-5MS （red）vs H-5MS （black）

NanoChrom BPTM GC Columns

Seven advantages:

1. Virtually minimal impact transitioning from another column brand

GC Columns

Seven Advantages ----------------------------------------- 42

USP GC Phases

Guide to Common Stationary Phase Selection

Petroleum and Petrochemicals ------------------------------------------- 54

ASTM Methods 48

Applications Comparisons of NanoChrom BP and JW columns ------------------------------------------ 52

Virtually minimal impact transitioning from another column brand

Excellent Column Inertness

Low Bleed Columns

Stable Stationary Phases

High Plate Number

Adequately Tuned Column Selectivity

Improved Results

Most GC column end users have used brand name GC columns for years, thus are reluctant to newcomer column products. In response, our aim is to ease the transition effort of column switching from a brand name to Nano-Chrom BP. In order to achieve this, NanoChrom fine tunes its NanoChrom BP stationary phases to yield similar or identical chromatographic performances to brand ones. NanoChrom carefully controls its manufacturing process. NanoChrom tests 100% all NanoChrom BP columns with the industry’s highest standard to ensure quality and performance of products delivery to our customers.

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------------------------------------------

NanoChrom BP Column product comprises of polysiloxane phases, polyethylene glycol phases, porous-layer-open tubular (PLOT) phases, low column bleed or MS (Mass Spec) grade columns across all stationary phases, and custom-made columns. NanoChrom BP Column is developed by an American company which own proprietary column technologies, manufacturing process, and high standard quality control. Each NanoChrom BP column is carefully prepared and signature made for quality and performance excellence to deliver to our customers worldwide.

NanoChrom BP stationary phases to yield similar or identical chromatographic performances to brand ones. NanoChrom BP columns have virtually minimal impact transitioning from another column brand.

NanoChrom BP-5MS （red）vs H-5MS （black） NanoChrom BP-5MS （red）vs H-5MS （black）

NanoChrom BPTM GC Columns

Seven advantages:

1. Virtually minimal impact transitioning from another column brand

GC Columns

Seven Advantages ----------------------------------------- 42

USP GC Phases

Petroleum and Petrochemicals ------------------------------------------- 54

ASTM Methods 48

Applications Comparisons of NanoChrom BP and JW columns ------------------------------------------ 52

Virtually minimal impact transitioning from another column brand

Excellent Column Inertness

Low Bleed Columns

Stable Stationary Phases

High Plate Number

Adequately Tuned Column Selectivity

Improved Results

Most GC column end users have used brand name GC columns for years, thus are reluctant to newcomer column products. In response, our aim is to ease the transition effort of column switching from a brand name to Nano-Chrom BP. In order to achieve this, NanoChrom fine tunes its NanoChrom BP stationary phases to yield similar or identical chromatographic performances to brand ones. NanoChrom carefully controls its manufacturing process. NanoChrom tests 100% all NanoChrom BP columns with the industry’s highest standard to ensure quality and performance of products delivery to our customers.

-----------------------------------------

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Our columns are designed and manufactured to achieve the highest level of inertness by a novel and proprietary surface deactivation technique. The column inertness is tested with probes of acid, bases and alcohol in our column test sample mix. NanoChrom BP columns excellent degree of inertness results in improved quantification accuracy and reliability for many challenging analyses. One example of such challenging analyses is EPA 8270.

The current industry trend is to push column detection limits and prolong column lifespans that demand lower column bleed and higher tempera-ture limits. NanoChrom BP low bleed columns are engineered with our rigorous process control and innovative cross-link technology. Almost all Nano-Chrom BP columns are rigorously tested for column bleed. Table I demonstrates the equivalent perfor-mance of NanoChrom BP-5MS column batch com-pared to other competitor columns.

We strive to achieve the highest quality GC column which, in turn, has led us to produce stable stationary phases to withstand extended temperature limits. NanoChrom BP columns are proprietary cross-linked and bonded, solvent rinseable, and have an extended lifetime. They have better oxidation resistance for many robust and challenging applications. Furthermore, NanoChrom BP columns have increased upper temperature limits, such as our PEG family columns, PLOT columns, and cyano-base VMS columns. We do not special label our column as ‘High Temp’, but our customers are enjoying our columns for application that requires oven temperature to 400ºC or above.

NanoChrom BP -5MS column bleeds

NanoChrom BP PLOT-Q Column bleed profile. Column, NanoChrom BP-PLOT Q, 0.53mm x 30m x 30um, Bleed temperature at 280ºC，15℃/min to 300 ℃(5min)

2. Excellent Column Inertness

3. Low Bleed Columns

Table 1： Column Bleed Comparison

Bleed Specifications

Temperature Limit (ºC)

<4pA @325ºC

-60 - 325/350

<4pA @320ºC

-60 - 325/350

<4pA @325ºC

-60 - 340/360

NanoChrom BP-5MS Brand H-5MS Brand D-5MS

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24[min]

NanoChrom BP-5MS Column Bleed

(30mx0.25mmx 0.25um, G1525-3002)

0-0.8 0.8-1.4 1.4-2 2-2.5 2.5-3 3-4 1-4 4-6 6-8 8-10 10-12 12-16

pA (325 ℃/350 ℃)

High column plate numbers improve many separations by increasing resolution and the detection limits. This is very important for applications of complex samples, such as EPA-8260 test gas mix, DHA of petroleum products such as gasoline, and environmental sample such as PAH.With our controllable process, we produce all NanoChrom BP columns with high plate numbers. NanoChrom routinely produces 100 meter x 0.25mm x 0.5um columns with more than 400,000 plate numbers. Such columns are widely used for DHA, PONA and VOC sample analyses. Another example of high plate number columns is NanoChrom BP- 624. NanoChrom BP-VMS columns that are used for EPA 8260, a complex separation application demanding high plate numbers. Also, our signature PLOT columns with high plate numbers exhibit improved separation results, as one of the many column benefits that many of our customers are recognizing and enjoying.

In addition to all the NanoChrom BP column attributes, our column selectivity is adequately tuned to meet a variety of application needs. Figure 2 shows the separation of residue solvents, isopropyl alcohol peak elutes before the dichloromethane peak in a pharmaceutical quality control. The MOH requires such an elution order for history reason. This separation can be easily improved on a NanoChrom BP 624 column from adequate column selectivity, with the same elution order.

Figure 2 Separation of IPA/DCM on a NanoChrom BP Inowax column. Column: 0.53mm x 30m x 1.0um.

4. Stable Stationary Phases

5. High Plate Number

6. Adequately Tuned Column Selectivity

0 2 3 4Time (min)

Our columns are designed and manufactured to achieve the highest level of inertness by a novel and proprietary surface deactivation technique. The column inertness is tested with probes of acid, bases and alcohol in our column test sample mix. NanoChrom BP columns excellent degree of inertness results in improved quantification accuracy and reliability for many challenging analyses. One example of such challenging analyses is EPA 8270.

The current industry trend is to push column detection limits and prolong column lifespans that demand lower column bleed and higher tempera-ture limits. NanoChrom BP low bleed columns are engineered with our rigorous process control and innovative cross-link technology. Almost all Nano-Chrom BP columns are rigorously tested for column bleed. Table I demonstrates the equivalent perfor-mance of NanoChrom BP-5MS column batch com-pared to other competitor columns.

We strive to achieve the highest quality GC column which, in turn, has led us to produce stable stationary phases to withstand extended temperature limits. NanoChrom BP columns are proprietary cross-linked and bonded, solvent rinseable, and have an extended lifetime. They have better oxidation resistance for many robust and challenging applications. Furthermore, NanoChrom BP columns have increased upper temperature limits, such as our PEG family columns, PLOT columns, and cyano-base VMS columns. We do not special label our column as ‘High Temp’, but our customers are enjoying our columns for application that requires oven temperature to 400ºC or above.

NanoChrom BP -5MS column bleeds

NanoChrom BP PLOT-Q Column bleed profile. Column, NanoChrom BP-PLOT Q, 0.53mm x 30m x 30um, Bleed temperature at 280ºC，15℃/min to 300 ℃(5min)

2. Excellent Column Inertness

3. Low Bleed Columns

Table 1： Column Bleed Comparison

Bleed Specifications

Temperature Limit (ºC)

<4pA @325ºC

-60 - 325/350

<4pA @320ºC

-60 - 325/350

<4pA @325ºC

-60 - 340/360

NanoChrom BP-5MS Brand H-5MS Brand D-5MS

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24[min]

NanoChrom BP-5MS Column Bleed

(30mx0.25mmx 0.25um, G1525-3002)

0-0.8 0.8-1.4 1.4-2 2-2.5 2.5-3 3-4 1-4 4-6 6-8 8-10 10-12 12-16

pA (325 ℃/350 ℃)

High column plate numbers improve many separations by increasing resolution and the detection limits. This is very important for applications of complex samples, such as EPA-8260 test gas mix, DHA of petroleum products such as gasoline, and environmental sample such as PAH.With our controllable process, we produce all NanoChrom BP columns with high plate numbers. NanoChrom routinely produces 100 meter x 0.25mm x 0.5um columns with more than 400,000 plate numbers. Such columns are widely used for DHA, PONA and VOC sample analyses. Another example of high plate number columns is NanoChrom BP- 624. NanoChrom BP-VMS columns that are used for EPA 8260, a complex separation application demanding high plate numbers. Also, our signature PLOT columns with high plate numbers exhibit improved separation results, as one of the many column benefits that many of our customers are recognizing and enjoying.

In addition to all the NanoChrom BP column attributes, our column selectivity is adequately tuned to meet a variety of application needs. Figure 2 shows the separation of residue solvents, isopropyl alcohol peak elutes before the dichloromethane peak in a pharmaceutical quality control. The MOH requires such an elution order for history reason. This separation can be easily improved on a NanoChrom BP 624 column from adequate column selectivity, with the same elution order.

Figure 2 Separation of IPA/DCM on a NanoChrom BP Inowax column. Column: 0.53mm x 30m x 1.0um.

4. Stable Stationary Phases

5. High Plate Number

6. Adequately Tuned Column Selectivity

0 2 3 4Time (min)

In combination with the a high degree of inertness, low bleed, high efficiency, and extended temperature range, NanoChrom BP columns can improve results for many challenging applications.

7. Improved Results

Polar，Crosslinked Polyethylene glycol (PEG) Applications：Acrylamide (Potato Chip Extract) EPA 602 Aromatics、Glycols, Glycols I、Peppermint Oil、Alcohols II and III、EPA-609 NitroAromatics、Hexamethylenediamine、Perfume、Aldehydes and Acids、Esters III Impurities in Ethylbenzene、Phenols III、Amines (low MW)、Ethylene Oxide、Impurities in Mixed Xylenes、Amines/Alcohols/Chlorides、FAME Standard I、Rapeseed oil、Analytes、FAMEs (Cocoa Butter) Impurities in Styrene、Rapid Analysis of Water in Organic Solvents、Aromatics II、Fast Styrene Analysis 、Industrial Solvent、Solvents I, II、Blood Alcohol 、Flavor Volatiles Lavender Oil Spiked with Camphor、Spearmint oil、Bourbon、Formalde-hyde Underivatized、Strawberry Syrup、Branched alcohols、Fragrance Reference Standard II 、Mushroom Aroma (Synthetic)、Sulfur and Selenium in Garlic by Headspace、BTEX、Frangnace Allergens、Nitrosamines、Tramanian Lavander Oil、Chlorinated Isooctane、Free Fatty Acids、Nutmeg Oil、Volatile Organics、Citronella Java Oil、 Organic Acids、Volatile SulfursSimilar Phases：HP-Innowax, DB-Wax, DB-Waxer, CP-Wax 52, VF-WaxRxt-Stabilwax, Rtx-Wax, Omega-Wax

30m×0.25mm×0.25um

30m×0.32mm×0.25um

30m×0.32mm×0.50um

30m x 0.32mm x1.0um

30m x 0.53mm x 1.00um

60m×0.25mm×0.25um

40 to 260/280

G2025-3001

G2032-3002

G2032-3005

G2032-3010

G2053-3010

G2025-6002

Nanochrom BP-INOWAX

Phase Applications TemperatureDescription

Non-polar,100% dimethyl polysiloxaneApplications：Aldehyde and Ketone、Inorganic Hydride Gases、Pyrethrins、Anabolic Steriods、Esters I、Kerosine Pyrolysates of Polystyrene、Anticonvul-sants、Ethylene Glycol Mixture、Pyrolysis of High density Polyethylene、Aromatics I、Eucalyptus Oil, Camphor, Menthol、Methanol in ethanol、Regular Unleaded Gasoline、Fatty Acids (Free)、Citronella Java Oil、Steroids: Sex Hormones、Common Industrial Solvents、Fragrance Reference Standard I、Organotin Compounds、Sulfur in Gasoline、Aldehyde and Ketone、EPA TO-14 Compounds、Inorganic Hydride Gases、Pyrethrins、Diesel Fuel Glycols III、Ozone Precursors- C2-C9 Gas Standard、Sulfur Compounds in Natural Gas、DNPH Derivative、Glycols/Diols、PFBHA Derivative、Synthetic Essential Oil Mixture、Drug Screen、Halogenated Hydrocarbons II、Polyethylene、Triethylene Glycol and Impurities、EPA Method 551、Hydrocarbons Sim-Dis、Inorganic Hydride Gases、Volatile AminesSimilar Phases：HP-1, DB-1,VF-1, Rtx-1, Rxi-1, SP-1,ZB-1

Non-polar，95% dimethyl/ 5% diphenyl Applications：Amphetamines and Precursors - TMSDerivative、CLP Pesticides、Herbicides、Organotin Compounds II、Antihistamines、Cocaine & Metabolites (TMS Derivatives)、Lemon Oil、Pain Killers - TMS Derivatives、Aroclors 1016-1268 (without 1221)、Cold Medication、Marijuana - TMS Derivatives、PBDEs、Bacterial Fatty Acid Methyl Esters、Cold-Pressed Orange Oil、Major Metabolites - TMS、Derivatives、PCBs by EPA Method 8083、Basic Drugs (Underivat-ized)、Common Drug Screen、Motor Oil、Phencycli-dine (PCP)、Benzodiazepines (Basic Drugs-Underivat-ized)、Congeners in DIN Method PCBs、Narcotics and Adulterants、Phytosterols (Saw Palmetto)、Bergamot Oil、Ethanolamines、Opiates (TMS Derivatives)、Steroids: Anabolic (Underivatized)、Butter Triglycerides、Ethyleneamines Organic Volatile Impurities、Sympathomimetic Amines Derivatives、Cannabinoids (TMS Derivatives)、European Red List Volatiles、Organochlorine Pesticides、Ylang Ylang OilSimilar Phases：HP-5, DB-5, VF-5MS, Rtx-5, Rxi-5, SP-5,ZB-5

30m×0.25mm×0.25um

30m x 0.25mm x 1.00um

30m×0.32mm×0.25um

60m×0.25mm×0.25um

30m×0.53mm×1.50um

30m×0.25mm×0.25um

60m×0.25mm×0.25um

30m×0.32mm×0.25um

30m x 0.32mm x 1.0um

30m x 0.53mm x 1.50um

-60 to 325/350

-60 to 300/320

G0525-3002

G0525-6002

G0532-3002

G0532-3010

G0553-3015

-60 to 325/350

-60 to 280/300

G0125-3002

G0125-3010

G0132-3002

G0125-6002

G0153-3015

Nanochrom BP-1

Nanochrom BP-5

Phase Applications TemperatureDescription P/N

Non-polar，100% dimethyl polysiloxaneApplications：Diesel / Gasoline、Ethylene Glycol Mixture、Pyrolysates of Polystyrene、Sulfur CompoundsSimilar Phases：HP-1MS, DB-1MS, CP-Sil 5CB MS, VF-1MS, Rtx-1MS, Rxi-1MS

Non-polar，95% dimethyl/ 5% diphenyl Applications：EPA, Alkaloids 、Fragrance Allergens、Polyethyleneamines、Amines and Nitriles、Gasoline Polynuclear AromaticHydrocarbons (PAHs)、Amphetamine and Methamphetamine、Halogenat-ed Compounds、Semivolatile Compounds、Antiepileptic 、Local Anesthetics、Semivolatile Organics、Basic Drugs、 Substituted AnilinesSimilar Phases：HP-5MS, DB-5MS, CP-Sil 8CB MS, VF-5MS, Rtx-5MS, Rxi-5MS, SP-5MS, ZB-5MS

30m×0.25mm×0.25um

30m×0.32mm×0.25um

30m x 0.25mm x 1.00um

30m×0.25mm×0.25um

30m×0.32mm×0.25um

-60 to 325/350

G1525-3002

G1532-3002

G1125-3002

G1132-3002

G1125-3010

Nanochrom BP-5MS

Nanochrom BP-1MS

Polar，PEG modified w/ acids Applications：Alcoholic Beverage Standard: Acids & Esters、Cresylic Acids、Fatty Acids (Free) Organic AcidsSimilar Phases：HP-FFAP, DB-FFAP, Rxt-Stabil-wa-DA, CP-FFAP

Nanochrom BP-FFAP

Nanochrom BP-1701

Nanochrom BP-50+MS

Nanochrom BP-35MS

Nanochrom BP-624

Mid-polar，65% dimethyl/ 35% diphenyl polysiloxane Applications：CLP2，Anilines、Cold Medication (Basic Drugs-Underivatized)、Organo Tins、Phenols、Barbiturates (Acidic/Neutral Drugs)、Endocrine Disruptors: Butyl Tins(hexyl deriva-tives)、Basic Drugs (Underivatized)、Organochlo-rine Pesticides IV、Primary Amines、Benzodiaze-pines、Chlordane、Ethanolamines、Toxaphene, EPA508、608、8081、8141、8151 Similar Phases：DB-35, DB-35MS, HP-35, Rtx-35, ZB-35,VF-35

Mid-polar，Poly(50% diphenyl, 50%dimethylsilox-ane)Applications：BHA and BHT、PAHs、Tocopherols、Butter Triglycerides、Free Steroids、PCBs、Triazine Herbicides II、EPA508、608、8081、8141、8151Similar Phases：DB-17, DB-17MS, HP-50+, CP-Sil 24CB, VF-17MS, Rxt-50, Rtx-17

Mid-polar，14% cyanopropylphenol/ 86% dimethyl polysiloxaneApplications：Antidepressants (Basic Drugs-Uncategorized)、Fentanyls、Phenoxy Acid Herbicide、Triazine Herbicides I、Antiepileptics (Uncategorized)、5% Fragrance Materials Association Mix、Styrene Impurities、VOCs in Packaging materialsSimilar Phases：DB-1701, Rtx-1701,CP-Sil 19CB, VF-1701

Mid-polar，Approximately 6% cyanopropylphenol/ 94% dimethyl polysiloxaneApplications：Air、Alcohols、water-drinking、FAME、DMA、water-underground、Halogenated Hydrocar-bons、DMSO、water-waste、Gasoline Esters、Packaging、Nitrogen Based Solvents II、Cosmetics、Residual Solvents, USP 467、Glycols II、Healthcare、Residue Solvents、Drinking、Volatile Amines、Tobacco Volatile、Petroleum Hydrocarbons (VPH) 503.1、Nutrition、Volatile SulfursEPA524.2、601、602、624、8010、8015 8240、8021、8020、504、8260Similar Phases：DB-624, DB-VRX, Rtx-624, Rtx-VMS, DB 502.2, VOCCol

30m×0.25mm×0.25um 40 to 260/280 G2125-3002

30m×0.25mm×0.25um 40 to 320/340 G3525-3002

30m×0.25mm×0.25um

30m×0.32mm×0.25um

40 to 320/340

G5025-3002

G5032-3002

30m×0.25mm×0.25um

30m×0.32mm×0.25um

30m×0.32mm×1.0um

-20 to 280/300

G6125-3002

G6132-3002

G6132-3010

30m×0.32mm×1.80um

30m×0.53mm×3.0um

-20 to 260

G6232-3018

G6253-3030

In combination with the a high degree of inertness, low bleed, high efficiency, and extended temperature range, NanoChrom BP columns can improve results for many challenging applications.

7. Improved Results

Polar，Crosslinked Polyethylene glycol (PEG) Applications：Acrylamide (Potato Chip Extract) EPA 602 Aromatics、Glycols, Glycols I、Peppermint Oil、Alcohols II and III、EPA-609 NitroAromatics、Hexamethylenediamine、Perfume、Aldehydes and Acids、Esters III Impurities in Ethylbenzene、Phenols III、Amines (low MW)、Ethylene Oxide、Impurities in Mixed Xylenes、Amines/Alcohols/Chlorides、FAME Standard I、Rapeseed oil、Analytes、FAMEs (Cocoa Butter) Impurities in Styrene、Rapid Analysis of Water in Organic Solvents、Aromatics II、Fast Styrene Analysis 、Industrial Solvent、Solvents I, II、Blood Alcohol 、Flavor Volatiles Lavender Oil Spiked with Camphor、Spearmint oil、Bourbon、Formalde-hyde Underivatized、Strawberry Syrup、Branched alcohols、Fragrance Reference Standard II 、Mushroom Aroma (Synthetic)、Sulfur and Selenium in Garlic by Headspace、BTEX、Frangnace Allergens、Nitrosamines、Tramanian Lavander Oil、Chlorinated Isooctane、Free Fatty Acids、Nutmeg Oil、Volatile Organics、Citronella Java Oil、 Organic Acids、Volatile SulfursSimilar Phases：HP-Innowax, DB-Wax, DB-Waxer, CP-Wax 52, VF-WaxRxt-Stabilwax, Rtx-Wax, Omega-Wax

30m×0.25mm×0.25um

30m×0.32mm×0.25um

30m×0.32mm×0.50um

30m x 0.32mm x1.0um

30m x 0.53mm x 1.00um

60m×0.25mm×0.25um

40 to 260/280

G2025-3001

G2032-3002

G2032-3005

G2032-3010

G2053-3010

G2025-6002

Nanochrom BP-INOWAX

Phase Applications TemperatureDescription

Non-polar,100% dimethyl polysiloxaneApplications：Aldehyde and Ketone、Inorganic Hydride Gases、Pyrethrins、Anabolic Steriods、Esters I、Kerosine Pyrolysates of Polystyrene、Anticonvul-sants、Ethylene Glycol Mixture、Pyrolysis of High density Polyethylene、Aromatics I、Eucalyptus Oil, Camphor, Menthol、Methanol in ethanol、Regular Unleaded Gasoline、Fatty Acids (Free)、Citronella Java Oil、Steroids: Sex Hormones、Common Industrial Solvents、Fragrance Reference Standard I、Organotin Compounds、Sulfur in Gasoline、Aldehyde and Ketone、EPA TO-14 Compounds、Inorganic Hydride Gases、Pyrethrins、Diesel Fuel Glycols III、Ozone Precursors- C2-C9 Gas Standard、Sulfur Compounds in Natural Gas、DNPH Derivative、Glycols/Diols、PFBHA Derivative、Synthetic Essential Oil Mixture、Drug Screen、Halogenated Hydrocarbons II、Polyethylene、Triethylene Glycol and Impurities、EPA Method 551、Hydrocarbons Sim-Dis、Inorganic Hydride Gases、Volatile AminesSimilar Phases：HP-1, DB-1,VF-1, Rtx-1, Rxi-1, SP-1,ZB-1

Non-polar，95% dimethyl/ 5% diphenyl Applications：Amphetamines and Precursors - TMSDerivative、CLP Pesticides、Herbicides、Organotin Compounds II、Antihistamines、Cocaine & Metabolites (TMS Derivatives)、Lemon Oil、Pain Killers - TMS Derivatives、Aroclors 1016-1268 (without 1221)、Cold Medication、Marijuana - TMS Derivatives、PBDEs、Bacterial Fatty Acid Methyl Esters、Cold-Pressed Orange Oil、Major Metabolites - TMS、Derivatives、PCBs by EPA Method 8083、Basic Drugs (Underivat-ized)、Common Drug Screen、Motor Oil、Phencycli-dine (PCP)、Benzodiazepines (Basic Drugs-Underivat-ized)、Congeners in DIN Method PCBs、Narcotics and Adulterants、Phytosterols (Saw Palmetto)、Bergamot Oil、Ethanolamines、Opiates (TMS Derivatives)、Steroids: Anabolic (Underivatized)、Butter Triglycerides、Ethyleneamines Organic Volatile Impurities、Sympathomimetic Amines Derivatives、Cannabinoids (TMS Derivatives)、European Red List Volatiles、Organochlorine Pesticides、Ylang Ylang OilSimilar Phases：HP-5, DB-5, VF-5MS, Rtx-5, Rxi-5, SP-5,ZB-5

30m×0.25mm×0.25um

30m x 0.25mm x 1.00um

30m×0.32mm×0.25um

60m×0.25mm×0.25um

30m×0.53mm×1.50um

30m×0.25mm×0.25um

60m×0.25mm×0.25um

30m×0.32mm×0.25um

30m x 0.32mm x 1.0um

30m x 0.53mm x 1.50um

-60 to 325/350

-60 to 300/320

G0525-3002

G0525-6002

G0532-3002

G0532-3010

G0553-3015

-60 to 325/350

-60 to 280/300

G0125-3002

G0125-3010

G0132-3002

G0125-6002

G0153-3015

Nanochrom BP-1

Nanochrom BP-5

Phase Applications TemperatureDescription P/N

Non-polar，100% dimethyl polysiloxaneApplications：Diesel / Gasoline、Ethylene Glycol Mixture、Pyrolysates of Polystyrene、Sulfur CompoundsSimilar Phases：HP-1MS, DB-1MS, CP-Sil 5CB MS, VF-1MS, Rtx-1MS, Rxi-1MS

Non-polar，95% dimethyl/ 5% diphenyl Applications：EPA, Alkaloids 、Fragrance Allergens、Polyethyleneamines、Amines and Nitriles、Gasoline Polynuclear AromaticHydrocarbons (PAHs)、Amphetamine and Methamphetamine、Halogenat-ed Compounds、Semivolatile Compounds、Antiepileptic 、Local Anesthetics、Semivolatile Organics、Basic Drugs、 Substituted AnilinesSimilar Phases：HP-5MS, DB-5MS, CP-Sil 8CB MS, VF-5MS, Rtx-5MS, Rxi-5MS, SP-5MS, ZB-5MS

30m×0.25mm×0.25um

30m×0.32mm×0.25um

30m x 0.25mm x 1.00um

30m×0.25mm×0.25um

30m×0.32mm×0.25um

-60 to 325/350

G1525-3002

G1532-3002

G1125-3002

G1132-3002

G1125-3010

Nanochrom BP-5MS

Nanochrom BP-1MS

Polar，PEG modified w/ acids Applications：Alcoholic Beverage Standard: Acids & Esters、Cresylic Acids、Fatty Acids (Free) Organic AcidsSimilar Phases：HP-FFAP, DB-FFAP, Rxt-Stabil-wa-DA, CP-FFAP

Nanochrom BP-FFAP

Nanochrom BP-1701

Nanochrom BP-50+MS

Nanochrom BP-35MS

Nanochrom BP-624

Mid-polar，65% dimethyl/ 35% diphenyl polysiloxane Applications：CLP2，Anilines、Cold Medication (Basic Drugs-Underivatized)、Organo Tins、Phenols、Barbiturates (Acidic/Neutral Drugs)、Endocrine Disruptors: Butyl Tins(hexyl deriva-tives)、Basic Drugs (Underivatized)、Organochlo-rine Pesticides IV、Primary Amines、Benzodiaze-pines、Chlordane、Ethanolamines、Toxaphene, EPA508、608、8081、8141、8151 Similar Phases：DB-35, DB-35MS, HP-35, Rtx-35, ZB-35,VF-35

Mid-polar，Poly(50% diphenyl, 50%dimethylsilox-ane)Applications：BHA and BHT、PAHs、Tocopherols、Butter Triglycerides、Free Steroids、PCBs、Triazine Herbicides II、EPA508、608、8081、8141、8151Similar Phases：DB-17, DB-17MS, HP-50+, CP-Sil 24CB, VF-17MS, Rxt-50, Rtx-17

Mid-polar，14% cyanopropylphenol/ 86% dimethyl polysiloxaneApplications：Antidepressants (Basic Drugs-Uncategorized)、Fentanyls、Phenoxy Acid Herbicide、Triazine Herbicides I、Antiepileptics (Uncategorized)、5% Fragrance Materials Association Mix、Styrene Impurities、VOCs in Packaging materialsSimilar Phases：DB-1701, Rtx-1701,CP-Sil 19CB, VF-1701

Mid-polar，Approximately 6% cyanopropylphenol/ 94% dimethyl polysiloxaneApplications：Air、Alcohols、water-drinking、FAME、DMA、water-underground、Halogenated Hydrocar-bons、DMSO、water-waste、Gasoline Esters、Packaging、Nitrogen Based Solvents II、Cosmetics、Residual Solvents, USP 467、Glycols II、Healthcare、Residue Solvents、Drinking、Volatile Amines、Tobacco Volatile、Petroleum Hydrocarbons (VPH) 503.1、Nutrition、Volatile SulfursEPA524.2、601、602、624、8010、8015 8240、8021、8020、504、8260Similar Phases：DB-624, DB-VRX, Rtx-624, Rtx-VMS, DB 502.2, VOCCol

30m×0.25mm×0.25um 40 to 260/280 G2125-3002

30m×0.25mm×0.25um 40 to 320/340 G3525-3002

30m×0.25mm×0.25um

30m×0.32mm×0.25um

40 to 320/340

G5025-3002

G5032-3002

30m×0.25mm×0.25um

30m×0.32mm×0.25um

30m×0.32mm×1.0um

-20 to 280/300

G6125-3002

G6132-3002

G6132-3010

30m×0.32mm×1.80um

30m×0.53mm×3.0um

-20 to 260

G6232-3018

G6253-3030

ASTM MethodsASTM

DesignationTesting Method

Method Title Part No.NanoChrom Column Recommendations

G8453-1550G8653-1530

G8153-3015

G8253-3015

G0153-3050G8353-3015

G0153-1008G0153-0508

G2053-3010G6253-3030

G2025-3002

G0125-6005

G2025-6002

G0153-3050

G8453-3050

G8532-6005

G8353-3015

G8353-5015

G2053-3010

G0132-6005

G6253-3030

G1132-3010

NanoChrom BP-PLOT MoleSieve, 15mx0.53mmx-50um NanoChrom BP-PLOT Q, 15mx0.53mmx30um

NanoChrom BP-1, 30mx0.53mmx5.0um NanoChrom BP-PLOT Al2O3 M, 30mx0.53mmx15um

NanoChrom BP-1, 10mx0.53mmx0.88um NanoChrom BP-1, 5mx0.53mmx0.88um

NanoChrom BP-InoWax, 30mx0.53mmx1.0um NanoChrom BP-624, 30mx0.53mmx3.0um

NanoChrom BP-PLOT MoleSieve, 30mx0.53mmx50um

NanoChrom BP-PLOT GasPro, 60mx0.32mmx5um

NanoChrom BP-PLOT Al2O3 M, 30mx0.53mmx-15um

NanoChrom BP-InoWax, 30mx0.53mmx1.0um

Contact NanoChrom for recommended VOC columns

NanoChrom BP-1, 60mx0.32mmx0.50um

NanoChrom BP-1MS, 30mx0.32mmx1.00um

NanoChrom BP-PLOT Al2O3 KCl, 30mx0.53mmx-15umNanoChrom BP-PLOT Al2O3 Na2SO4, 30mx-0.53mmx15um

Standard test method for the analysis of natural gas

Standard test method for the analysis of reformed gas

Standard test method for the analysis of liquified petroleum gases and propene concentration

Standard test method for the analysis of high purity n-heptane and iso-octane

Standard test method for C8 aromatic hydrocarbons

Standard test method for the butadiene dimer and styrene in butadiene concentration

Standard test method for determina-tion of C2 through C5 hydrocarbons in gasoline

Standard test method for non-con-densable gases in C2 and for lighter hydrocarbon products

Standard test method for other hydrocarbons and carbon dioxide in high-purity ethylene

Standard test method for butadiene purity and hydrocarbon impurities

Standard test method for hydrocarbon traces in concentrated propylene

Standard test method for the purity of methyl ethyl ketone

Standard test method for analysis of the boiling range distribution of petroleumfractions to C60

Standard practice for measuring volatile organic matter in water

Standard test method for the analysis of unreacted toluene diisocyanates in urethane prepolymers and coating solutions

Standard test method for the purity of methyl isobutyl ketone

Standard test method for the analysis of aromatics in mineral spirits

Standard test method for analysis of cyclohexane

Standard test method for the analysis of fatty acid compositions

D 1945

D 1946

D 1983

D 2163

D 2268

D 2306

D 2426

D 2427

D 2504

D 2505

D 2593

D 2712

D 2804

D 2908

D 3054

D 3257

D 3329

D 3432

Extended D 2887

GLC of methyl ether

Capillary GC

Aqueous-injection GC

United States Pharmacopoeia (USP) GC Phases

Equivalent NanoChrom BP ColumnUSP Code

USP Composite

NanoChrom BP-1NanoChrom BP-1MS

NanoChrom BP-InowaxNanoChrom BP- CarboWax20M

NanoChrom BP-CarboWax20M

NanoChrom BP-InoWax

NanoChrom BP-FFAP

NanoChrom BP-35MS

NanoChrom BP-FFAP

NanoChrom BP-InoWax

NanoChrom BP-5MS

NanoChrom BP-35MS

NanoChrom BP-1301

NanoChrom BP-PLOT U

NanoChrom BP-1701

NanoChrom BP-50+ MS

NanoChrom BP-23

NanoChrom BP-225

NanoChrom BP-FAME

NanoChrom BP-InoWax

Dimethylpolysiloxane, oil

Dimethylpolysiloxane gum

Polyethylene glycol, average MW 950--1,050

Polyethylene glycol, average MW 3,000--3,700

Polyethylene glycol, average MW 15,000

Poly(75% diphenyl, 25%dimethylsiloxane)

Polyethylene glycol, average MW 380-420

Polyethylene glycol, TPA (Carbowax 20M, Terephthalic acid)

5% phenyl 95% methylpolysiloxane

20% phenylmethyl 80% Dimethylpolysiloxane

Polyethylene glycol & diepoxide esterified with nitroterepthalic acid

1% vinyle 5% phenylmethylpolysiloxane

Phase G1 plus tailing inhibitor

Polyethylene glycol average MW 1500

Phenylmethyldimethylsilicone (10% phenyl substituted)

35% diphenyl 65% Dimethylvinylsiloxane

6% Cyanopropylphenyl-94% dimethylpolysiloxane

Divinylbenzene ethylene glycol dimethacrylate

14% Cyanopropylphenyl-86% methylpolysiloxane

80% Bis(3-cyanopropyl)20% 3-cyanopropylphenylpolysiloxane or 90% 3-cyanopropyl 10% phenylmethylsiloxane

50% phenyl, 50% methylpolysiloxane

3-cyanopropylmethylpolysiloxane

50% cyanopropylmethyl 50% phenylmethylpolysiloxane

ASTM MethodsASTM

G8453-1550G8653-1530

G8153-3015

G8253-3015

G0153-3050G8353-3015

G0153-1008G0153-0508

G2053-3010G6253-3030

G2025-3002

G0125-6005

G2025-6002

G0153-3050

G8453-3050

G8532-6005

G8353-3015

G8353-5015

G2053-3010

G0132-6005

G6253-3030

G1132-3010

NanoChrom BP-PLOT MoleSieve, 15mx0.53mmx-50um NanoChrom BP-PLOT Q, 15mx0.53mmx30um

NanoChrom BP-1, 30mx0.53mmx5.0um NanoChrom BP-PLOT Al2O3 M, 30mx0.53mmx15um

NanoChrom BP-1, 10mx0.53mmx0.88um NanoChrom BP-1, 5mx0.53mmx0.88um

NanoChrom BP-PLOT MoleSieve, 30mx0.53mmx50um

Contact NanoChrom for recommended VOC columns

NanoChrom BP-PLOT Al2O3 KCl, 30mx0.53mmx-15umNanoChrom BP-PLOT Al2O3 Na2SO4, 30mx-0.53mmx15um

Standard test method for the analysis of natural gas

Standard test method for the analysis of reformed gas

Standard test method for the analysis of liquified petroleum gases and propene concentration

Standard test method for the analysis of high purity n-heptane and iso-octane

Standard test method for C8 aromatic hydrocarbons

Standard test method for the butadiene dimer and styrene in butadiene concentration

Standard test method for determina-tion of C2 through C5 hydrocarbons in gasoline

Standard test method for non-con-densable gases in C2 and for lighter hydrocarbon products

Standard test method for other hydrocarbons and carbon dioxide in high-purity ethylene

Standard test method for butadiene purity and hydrocarbon impurities

Standard test method for hydrocarbon traces in concentrated propylene

Standard test method for the purity of methyl ethyl ketone

Standard test method for analysis of the boiling range distribution of petroleumfractions to C60

Standard practice for measuring volatile organic matter in water

Standard test method for the analysis of unreacted toluene diisocyanates in urethane prepolymers and coating solutions

Standard test method for the purity of methyl isobutyl ketone

Standard test method for the analysis of aromatics in mineral spirits

Standard test method for analysis of cyclohexane

Standard test method for the analysis of fatty acid compositions

D 1945

D 1946

D 1983

D 2163

D 2268

D 2306

D 2426

D 2427

D 2504

D 2505

D 2593

D 2712

D 2804

D 2908

D 3054

D 3257

D 3329

D 3432

Extended D 2887

GLC of methyl ether

Capillary GC

Aqueous-injection GC

United States Pharmacopoeia (USP) GC Phases

Equivalent NanoChrom BP ColumnUSP Code

USP Composite

NanoChrom BP-InowaxNanoChrom BP- CarboWax20M

NanoChrom BP-CarboWax20M

NanoChrom BP-InoWax

NanoChrom BP-FFAP

NanoChrom BP-35MS

NanoChrom BP-FFAP

NanoChrom BP-InoWax

NanoChrom BP-5MS

NanoChrom BP-35MS

NanoChrom BP-1301

NanoChrom BP-PLOT U

NanoChrom BP-1701

NanoChrom BP-50+ MS

NanoChrom BP-23

NanoChrom BP-225

NanoChrom BP-FAME

NanoChrom BP-InoWax

Dimethylpolysiloxane, oil

Dimethylpolysiloxane gum

Polyethylene glycol, average MW 950--1,050

Polyethylene glycol, average MW 3,000--3,700

Polyethylene glycol, average MW 15,000

Poly(75% diphenyl, 25%dimethylsiloxane)

Polyethylene glycol, average MW 380-420

Polyethylene glycol, TPA (Carbowax 20M, Terephthalic acid)

20% phenylmethyl 80% Dimethylpolysiloxane

Polyethylene glycol & diepoxide esterified with nitroterepthalic acid

1% vinyle 5% phenylmethylpolysiloxane

Phase G1 plus tailing inhibitor

Polyethylene glycol average MW 1500

Phenylmethyldimethylsilicone (10% phenyl substituted)

35% diphenyl 65% Dimethylvinylsiloxane

6% Cyanopropylphenyl-94% dimethylpolysiloxane

Divinylbenzene ethylene glycol dimethacrylate

14% Cyanopropylphenyl-86% methylpolysiloxane

80% Bis(3-cyanopropyl)20% 3-cyanopropylphenylpolysiloxane or 90% 3-cyanopropyl 10% phenylmethylsiloxane

50% phenyl, 50% methylpolysiloxane

3-cyanopropylmethylpolysiloxane

50% cyanopropylmethyl 50% phenylmethylpolysiloxane

Standard test method for the purity of halogentated organic solvents

NanoChrom BP-FFAP, 30mx0.32mmx0.25um

NanoChrom BP-PLOT Q, 15mx0.53mmx30um

NanoChrom BP-InoWax, 30mx0.53mmx1.0umNanoChrom BP-PLOT Q，30mx0.53mmx30um

NanoChrom BP-1 ,15mx0.53mmx5.0umNanoChrom BP-InoWax, 30mx0.32mmx0.25um

Standard test method for the analysis of PCB's in water

Standard test method for the analysis of alcohol content and the purity of acetate esters

Standard practice for the analysis of collected organic vapors

Standard test method for theanalysis of volatile alcohols in water

Standard test method for the analysis of isopropylben-zene (Cumene)

Standard test method for the analysis of o-xylene

Standard test method for the analysis of p-xylene

Standard test method for the analysis of purgeable organic coumpounds in water

Standard test method for thepurity of methyl amyl ketone and methyl isoamyl ketone

Standard test method for theanalysis of hydrocarbons withlow molecular weights in waterStandard test method for the determination of dimers in acrylic acid

Standard test method forbutylene analyses

Standard test method for theresidual vinyl chloride monomer content in PPB in vinyl chloride homo- and co-polymers

Standard test method for the determination of traces of methanol in propylene concen-

Standard test method for the analysis of chlordane and heptachlor residues in indoor airStandard test method for the analysis of major organic impurities in phenol producedby the cumene process

Standard test method for ethyl methyl pentonal content and the purity value of 2-ethylhexanol

Standard test method for theanalysis of cyclohexylaninemorpholine,diethylaminoethanol in water and condensed steam

NanoChrom BP-PLOT Al2O3 “S,50mx0.53mmx15um

The activated charcoal tube adsorption method

Direct aqueous injection GC

Headspace sam-pling

Headspace GC

Direct aqueousinjection GC

D 3447

D 3534

D 3545

D 3687

D 3695

D 3760

D 3797

D 3798

D 3871

D 3893

D 3973

D 4415

D 4424

D 4443

D 4864

D 4947

D 4961

D 4983

D 5008

G6253-3030

G1132-3010

G6253-3030

G2053-3010

G2032-6005

G6253-3030

G0153-3026

G2132-3002

G8253-5015

G0153-3026

G2032-6002 G0132-5005

G0153-1550 G2032-3002

G2053-3010 G8653-3030

G0553-3015

G8653-1530

G1532-3010

Standard test method for determining the impurities in high-purity ethylbenzene

Standard test method for the analysis of nicotine in indoor air

Standard test method for the analysis of sryreme

Standard test method for the analysis of carbonyl sulfide in propylene

Standard test method the determina-tion of the boiling range distribution of crude

Standard test method for the analysis of tar acid composition

Standard test method for the determination of chlorinated organic acid compounds in water

Standard test method for the analysis of methyl tert-butyl ether (MTBE)

Standard test method for the analysis of petroleum waxe

Standard test method for the analysis of nitrogen and phosphorus-contain-ing

Standard test method for thedetermination of ethanol content in denatured fuel ethanol

Standard test method for the determination of trace organic impurities in monomer grade vinyl

Standard test method for the determination of residual acryloni-trile monomers in

Standard test method for the determination of oxygenates in gasoline

Standard test method for analysis of sulfur compounds in light petroleum liquids

Standard test method for the determination of benzene, toluene, ethylbenzene, p/m-xylene, C9, and heavier aromatics, and total aromatics in finished gasoline

Standard test method for the determination of 1,1-trichloroethane and methylene

Standard test method for 1,2-dibromoethane and 1,2-dibro-mo-3-chloropropane in water

D 5060

D 5075

D 5135

D 5303

D 5307

D 5310

D 5316

D 5317

D 5320

D 5441

D 5442

D 5475

D 5501

D 5507

D 5508

D 5580

D 5599

D 5623

G6253-3030

GC with an electron capturedetector

GC with a nitro-gen phosphorus detectr

GC and oxygenselec-tive flameioniza-tion

GC and sulfur selective detection

Microex tractionand GC

Capillary GC

Capillary column/-multidi-mensional GC

Headspace- capillary GC

NanoChrom BP-1, 7.5mx0.53mmx5.0um

NanoChrom BP-5, 30m x0.53mmx1.5um NanoChrom BP-5, 30mx0.32mmx1.0um

NanoChrom BP-InoWax, 60mx0.32mmx0.5umNanoChrom BP-5, 30m x0.53mmx1.5um NanoChrom BP-5, 30mx0.32mmx1.0um

NanoChrom BP-1MS, 30mx0.32mmx1.0umNanoChrom BP-PLOT Q, 30mx0.53mmx30um

NanoChrom BP-5MS, 30mx0.25mmx0.25um NanoChrom BP-1701, 30mx0.25mmx0.25umNanoChrom BP-35MS, 30mx0.25mmx0.25um

NanoChrom BP-5MS, 30mx0.25mmx0.25um NanoChrom BP-35MS, 30mx0.25mmx0.25um NanoChrom BP-1701, 30mx0.25mmx0.25um

NanoChrom BP-1, 30mx0.53mmx3.0umNanoChrom BP-624, 30mx0.32mmx1.8um

NanoChrom BP-PLOT Q, 15mx0.53mmx30umNanoChrom BP-PLOT U, 30mx0.53mmx20um

NanoChrom BP-1, 30mx0.53 mmx5.0um

G0553-3015G0532-3010

G2032-6005G0553-3015G0532-3010

G8532-3005

G0153-0750

G1525-3002

G1132-3010 G8653-3030

G1525-3002 G6125-3002 G3525-3002

G0153-3030 G6232-3018

G8653-3030

G0525-1502

G1525-3002 G3525-3002 G6125-3002

G0125-1005

G8653-1530 G8753-3020

G8653-3030

G0153-3050

G0125-6010

G0132-3040

ASTM Designation

Testing Method Method Title Part No.NanoChrom Column Recommendations

ASTM MethodsASTM

ASTM Methods