2015 IEM CAC poster V1.0
1
IEM Retreat and Conference 9/21/2015 IEM Cancer Animal Core Qi Shao 1 and John Bischof 1, 2 1 Department Biomedical Engineering; 2 Department of Mechanical Engineering Service The Animal Cancer Core Lab is an IEM supported resource for faculty and local industry to engage more readily in cancer animal research. The lab can dramatically reduce the time and effort for an investigator to run pilot and long term projects using cancer models that are already in operation within the core. Services Offered: Animal housing and management Animal handling Cell culture Tumor implantation Tumor monitoring Controlled substance management Cancer models Tumor transplantation from cancer cells [1] Dorsal skinfold chamber on mice [2] Rodent/strain Tumor/Genetic Description Mouse (nude) LNCaP-Pro5 Human, prostate cancer Mouse (nude) MCF-7 Human, breast cancer Mouse (nude) ELT-3 Rodent, uterine leiomyoma Mouse (nude) AsPC-1 Human, pancreatic cancer Mouse (nude) MDA-MB-231 Human, breast cancer Mouse (nude) MDA-MB-435A Human, breast cancer (melanoma) Mouse (C57BL/6) TRAMP-C2 Mouse, prostate cancer Mouse (A/J) SCK Mouse, mammary carcinoma Mouse (C3H) FSaII Mouse, fibrosarcoma Rat (Copenhagen) Dunning AT-1 Rat, prostate cancer Imaging and Assessment protocols Assessment Method Outcome DSFC Imaging Bright field Inflammation: WBC Rolling Imaging Infra-red Surface temperature change Imaging Fluorescence Perfusion Defect Histology Immunohistochemistry Inflammation: NF-kB,VCAM, CD31 Therapy Thermal, chemical, electrical and mechanical Histology and Perfusion Defect Hindlimb Imaging Photoacoustic lifetime Tissue oxygenation Imaging Infra-red Surface temperature Imaging Contrast enhanced ultrasound Blood Flow Index (Vascularity) Imaging Dual mode ultrasound array Treatment and imaging Imaging SWIFT MRI IONP Nanoparticle Biodistribution Imaging Contrast Enhanced MR Vascular Permeability and Flow Change Mass Spec Atomic Emission Spectroscopy Nanoparticle biodistribution Perfusion Microbead assay Vascular blood pool in organ/tumor over time Histology Immunohistochemistry Inflammation: NF-kB,VCAM, CD31 Therapy Tumor Growth Delay Treatment outcome metric Budget Service Cost 1 Description Major sources of cost Basic small animal study $500 IACUC protocol Controlled substance handling Conventional lab mice for pilot study Animal transportation Experiment technical support Lab animals order and shipping Animal housing and care (by RAR) Graduate assistant working hour Histology service cost Small animal cancer model 2 $1,500 Includes all basic service and Cancer cells culture Hindlimb tumor on mouse 3 Tumor growth monitor Additional animal cost Cell culture, cell preparation Tumor implantation Additional working hours DSFC model $2,200 Includes all basic service and: DSFC surgery Equipment wear Consumable surgical products Additional hours on surgery Additional options 4 TBD Rat (weight 200-500 g) Metastasis model Special cancer model Wound healing Etc. TBD Note: 1 Estimated unit price given as 1 cage of 4 mice, costs vary with actual study 2 Cost given as example of LNCaP pro 5 prostate cancer, other cancer types are also available, please refer to the proposal 3 Mouse with either one-sided or both-sided tumor, tumor size ranges 50-500 mm 3 , diameter 2-15 mm 3 4 Subject to IACUC approval More about IEM CAC http://www.me.umn.edu/labs/IEM_CAC/index.shtml, or scan it → Facilities: Investigator Managed Housing Area (IMHA) in Diehl Hall G-138 General purpose lab in Mechanical Engineering 3120 People: John Bischof, director, [email protected] Qi Shao, lab manager (CEO, CFO, COO, CTO, CIO and CMO), [email protected] Supported projects since 2014 IRE treatment setup and electrode design. [4] PALI tracking of pO 2 during breathing modulation in the upper part of the hindlimb of a normal mouse.[3] SWIFT MR imaging in a mouse hindlimb tumor model. [5] GRE, SWIFT, SWIFT T 1 , and SWIFT R 1 map at three different IONP concentrations in mice. [7] An in-vivo mouse model study tracking the tumor growth. [6] Conductivity mapping by MR-EPT on a AT-1 tumor bearing rat. [8] Images of murine hindlimb LNCaP tumors injected with IONPs or ms- IONPs. [10] Schematic diagram of magnetic nanoparticle imaging using magneto acoustic tomography method with a short pulsed magnetic field. [9] Cancer therapy and ablation research Assessment of microvascular shutdown following a therapeutic intervention in cancer. [11] Nanoparticle preconditioning enhances thermal injury in DSFC LNCaP tumors. [12] SCK tumor perfusion defects imaged using contrast enhanced ultrasonography. [13] In vivo photoacoustic lifetime imaging for tissue oxygen imaging.[14] DCE MRI of LNCaP hindlimb tumors after nanodrug (NP-TNF) injection. [11] References [1] Dranoff, Nature Reviews Immunology, 2012 [2] Wingenfeld et al. The Journal of Bone and Joint Surgery, 2002 [3] Shao et al. Journal of Biomedical Optics, 2015 [4] Jiang et al. Annual of biomedical engineering, 2014 [5] Etheridge et al. Technology, 2014 [6] Yu et al. Manuscript in preparation. [7] Zhang et al. Manuscript in preparation. [8] Liu et al. Manuscript in preparation. [9] Mariappan et al. Manuscript submitted, in revision. [10] Hurley et al. Manuscript submitted, in revision. [11] Shenoi et al. Molecular Pharmaceutics, 2013. [12] Shenoi et al. Nanomedicine, 2011 [13] Visaria et al. Molecular Cancer Therapeutics, 2006 [14] Shao et al. Journal of Biomedical Optics, 2013
Transcript of 2015 IEM CAC poster V1.0
IEM Retreat and Conference 9/21/2015
IEM Cancer Animal Core Qi Shao1 and John Bischof1, 2
1Department Biomedical Engineering; 2Department of Mechanical Engineering
Service
The Animal Cancer Core Lab is an IEM supported resource for faculty and local industry
to engage more readily in cancer animal research. The lab can dramatically reduce the
time and effort for an investigator to run pilot and long term projects using cancer models
that are already in operation within the core.
Services Offered:
Animal housing
and management Animal handling
Cell culture Tumor
implantation
Tumor
monitoring
Controlled
substance
management
Cancer models
Tumor transplantation from cancer cells [1] Dorsal skinfold chamber on mice [2]
Rodent/strain Tumor/Genetic Description
Mouse (nude) LNCaP-Pro5 Human, prostate cancer
Mouse (nude) MCF-7 Human, breast cancer
Mouse (nude) ELT-3 Rodent, uterine leiomyoma
Mouse (nude) AsPC-1 Human, pancreatic cancer
Mouse (nude) MDA-MB-231 Human, breast cancer
Mouse (nude) MDA-MB-435A Human, breast cancer (melanoma)
Mouse (C57BL/6) TRAMP-C2 Mouse, prostate cancer
Mouse (A/J) SCK Mouse, mammary carcinoma
Mouse (C3H) FSaII Mouse, fibrosarcoma
Rat (Copenhagen) Dunning AT-1 Rat, prostate cancer
Imaging and Assessment protocols
Assessment Method Outcome
DSFC
Imaging Bright field Inflammation: WBC Rolling
Imaging Infra-red Surface temperature change
Imaging Fluorescence Perfusion Defect
Histology Immunohistochemistry Inflammation: NF-kB,VCAM, CD31
Therapy Thermal, chemical, electrical and
mechanical Histology and Perfusion Defect
Hindlimb
Imaging Photoacoustic lifetime Tissue oxygenation
Imaging Infra-red Surface temperature
Imaging Contrast enhanced ultrasound Blood Flow Index (Vascularity)
Imaging Dual mode ultrasound array Treatment and imaging
Imaging SWIFT MRI IONP Nanoparticle Biodistribution
Imaging Contrast Enhanced MR Vascular Permeability and Flow Change
Mass Spec Atomic Emission Spectroscopy Nanoparticle biodistribution
Perfusion Microbead assay Vascular blood pool in organ/tumor over
time
Histology Immunohistochemistry Inflammation: NF-kB,VCAM, CD31
Therapy Tumor Growth Delay Treatment outcome metric
Budget
Service Cost1 Description Major sources of cost
Basic small
animal study $500
IACUC protocol
Controlled substance handling
Conventional lab mice for pilot
study
Animal transportation
Experiment technical support
Lab animals order and shipping
Animal housing and care (by RAR)
Graduate assistant working hour
Histology service cost
Small animal
cancer model2 $1,500
Includes all basic service and
Cancer cells culture
Hindlimb tumor on mouse3
Tumor growth monitor
Additional animal cost
Cell culture, cell preparation
Tumor implantation
Additional working hours
DSFC model $2,200 Includes all basic service and:
DSFC surgery
Equipment wear
Consumable surgical products
Additional hours on surgery
Additional
options4 TBD
Rat (weight 200-500 g)
Metastasis model
Special cancer model
Wound healing
Etc.
TBD
Note: 1 Estimated unit price given as 1 cage of 4 mice, costs vary with actual study 2 Cost given as example of LNCaP pro 5 prostate cancer, other cancer types are also available, please refer to the proposal 3 Mouse with either one-sided or both-sided tumor, tumor size ranges 50-500 mm3, diameter 2-15 mm3 4 Subject to IACUC approval
More about IEM CAC
http://www.me.umn.edu/labs/IEM_CAC/index.shtml, or scan it →
Facilities:
Investigator Managed Housing Area (IMHA) in Diehl Hall G-138
General purpose lab in Mechanical Engineering 3120
People:
John Bischof, director, [email protected]
Qi Shao, lab manager (CEO, CFO, COO, CTO, CIO and CMO),
Supported projects since 2014
IRE treatment setup and electrode design. [4] PALI tracking of pO2 during breathing modulation in the upper part of the hindlimb of a
normal mouse.[3]
SWIFT MR imaging in a mouse hindlimb tumor
model. [5]
GRE, SWIFT, SWIFT T1, and SWIFT R1 map at
three different IONP concentrations in mice. [7]
An in-vivo mouse model study tracking the
tumor growth. [6]
Conductivity mapping by MR-EPT on a AT-1 tumor
bearing rat. [8]
Images of murine hindlimb LNCaP
tumors injected with IONPs or ms-
IONPs. [10]
Schematic diagram of magnetic nanoparticle imaging
using magneto acoustic tomography method with a
short pulsed magnetic field. [9]
Cancer therapy and ablation research
Assessment of microvascular shutdown following a therapeutic
intervention in cancer. [11] Nanoparticle preconditioning enhances thermal injury in DSFC LNCaP
tumors. [12]
SCK tumor perfusion defects imaged using contrast enhanced
ultrasonography. [13]
In vivo photoacoustic lifetime imaging
for tissue oxygen imaging.[14] DCE MRI of LNCaP hindlimb tumors
after nanodrug (NP-TNF) injection. [11]
References [1] Dranoff, Nature Reviews Immunology, 2012 [2] Wingenfeld et al. The Journal of Bone and Joint Surgery, 2002
[3] Shao et al. Journal of Biomedical Optics, 2015 [4] Jiang et al. Annual of biomedical engineering, 2014
[5] Etheridge et al. Technology, 2014 [6] Yu et al. Manuscript in preparation.
[7] Zhang et al. Manuscript in preparation. [8] Liu et al. Manuscript in preparation.
[9] Mariappan et al. Manuscript submitted, in revision. [10] Hurley et al. Manuscript submitted, in revision.
[11] Shenoi et al. Molecular Pharmaceutics, 2013. [12] Shenoi et al. Nanomedicine, 2011
[13] Visaria et al. Molecular Cancer Therapeutics, 2006 [14] Shao et al. Journal of Biomedical Optics, 2013
