2011 IMB Orientation For newcomers 2006-11 Thayumanasamy Somasundaram With contributions from: Mr....

2011 IMB OrientationFor newcomers

2006-11 Thayumanasamy Somasundaram

With contributions from:

Mr. W. Baggett, Mr. E. Kirkland, Mr. R. Wang, Ms. J. Dodge (EH&S), Dr. J. Hare, Dr. T. Logan, Dr. C. Mundoma, Dr. K. Maddox, and Dr. M. Zawrotny

Overview

04/19/23© 2006-10 Thayumanasamy Somasundaram2

Inform you about people and contactsIssues related to Kasha Lab (KLB)

Review general and wet lab practicesRemind you about correct procedures

Introduce you to procedures for usingCommon Labs Core Facilities

Supplement to FSU Safety SeminarsGive you some computer tips

Emergency Contacts


Env. Health & SafetyMain: 4-6895Lab Safety: 4-8916Bio Safety: 4-5374Rad Safety: 4-8802Chem Safety: 4-

7682

FSUEmergency: 911Urgency: 311Police: 4-1234Maintenance: 4-2424Information: 4-4636

To reach a campus number simply dial 4- or 5- followed the last four digits. To reach an outside number dial 9- followed by the last seven digits.

Location of Emergency Help

04/19/23© 2006-10 Thayumanasamy Somasundaram

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EXIT Lab Contacts

First AidFire

Extinguisher

If a spill occurs . . .


Inform EH & Safety OfficeAll spill over ½ liter or 1 pound of Haz

material need to be reported to Safety Office (4-6895)

If you can clean it do soWithout injuring yourself or othersInform your PI and SomaIf you can’t clean it, let EH & S handle it

If you get hurt . . .


Depending upon the situationCall 911 and seek assistanceCoworker should stay with injuredOnce you are okay

Let your PI and Regina Ware knowLet Safety Office knowWith-in 48 hour we need to fill some paper work

(students and employees)

Emergency Help


Drench Hose Eye Wash & Shower

In Every Lab In Hallway

Emergency Help


Fire Extinguisher

Fire Pull Station

In Hallway In Hallway

Helpful People: Fiscal

Pay, Visa, & State Budget Regina Ware (KLB 118)

Res. Foundation & Travel: Patricia Brinkley (KLB 117)

Contracts and Grants: Rosalind Sapp (KLB 116)

Appointments: Regina Ware (KLB 118)


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Helpful People: General

Building & Common Lab:Wade Baggett or Ed Kirkland (KLB 121 & 123)

Electronic, electrical & printer: Rick Wang (KLB 119)

MOB Program: Denise Newsome (KLB 106)


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General Lab Etiquette


No food or drink in the labsWear proper attire, eye, and foot wareWork under a fume hoodKeep the cylinders strappedUse ice machine for samples onlyInform others while working late

Wet Lab Etiquettes


Gloves UsageUse correct type: Nitrile, Latex, & VinylDisposable gloves are resistantBut not imperviousUse only inside the labDiscard before using common equipmentsChange often

Proper Gloves Usage


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YESYES

NONO

NONO

NONOX

XX

Proper Fume Hood & Info


Sharps & Broken Glass Disposal


Follow proper sharps disposal procedureUse a designated unit or strong-lined marked boxReplace when 75% fullCall EH & S to pick-up

Follow proper broken glass disposal procedureDesignated unit or strong-lined marked cardboard

unitReplace when 75% fullCustodians will remove only broken-glass disposal

Disposal Units (Ideal)


Sharps Disposal Unit

Glass Disposal Unit

EH & S Can Provide Some Disposal Units

Needles, Razors, Syringes, etc.,

Bottles, Beakers, Test Tubes, etc.,

Hazardous Waste Etiquette


No hazardous chem in drain or sinkNo hazardous waste in trashWaste containers from EH & SLabel samples with name, date &

chemicalCall EH & S for pick-up Pick-up requests via the Web

Chemical Waste Containers


Liquid Waste Unit

Waste Disposal Unit

Name | Date

Chemical

Name | Date

Chemical

Biological Samples


Bacterial waste > Autoclavable waste Bacterial tips > Autoclavable wasteAutoclave when 75% fullAutoclaved waste > Regular trashOther waste > Regular trashDO NOT mix in autoclave bagIn doubt? Check w/ Dr. Hare or EH&S

Radioactive Samples


Permission & Radiation Safety Training required

PEF and Li labsSeparate and dedicated areasProper storage, disposal, and log required

Radiation Safety

Radiation safety training required

Personal radiation monitor (optional)

X-Ray Facility in KLB 410


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Shops and Labs


Machine ShopWood ShopElectronics ShopCommon Labs

Machine & Wood Shops


KLB 121Wade Baggett

(4-1419)MachiningCommon repairsBuilding issuesUser training

KLB 123Ed Kirkland

(5-1338)Wood cabinetsBook shelvesTables & desksCommon repairs

Electronics Shop


KLB 119Rick Wang (5-3309)Solve minor electrical problemsDo electronics troubleshootingRepair instrumentsProvide projector and printer help

Common Equipment Labs


Ice machines: W Baggett

Ultra colds: Rick WangNano-pure: Rick WangSpeed vac: Rick Wang

Web page: www.sb.fsu.edu/core/cel/cel.html

KLB 211, 212, 511, & 512Usage Requires Special Training

Autoclaves: Wade Baggett Centrifuges: Rick Wang Micro Fluidizer: W Baggett French Press: W Baggett

Core: Computer Resources (CRF)


KLB Basement & Common Areas

Director: Dr. Michael [email protected]

Hardware and core software installation

Computer connectivity and virus issuesE-mail and IMB web site issuesData archiving consultationURL: www.sb.fsu.edu/core/crf/crf.html

Computer Resources(CRF)


User subscribable mail [email protected] [Everyone][email protected] [PBF users][email protected] [PEF users][email protected] [Xray users]

To subscribe: https://lists.fsu.edu/mailman/listinfo/imbpeople

To view archives: https://lists.fsu.edu/pipermail/imbpeople

Core: Physical Biochemistry Facility (PBF)


KLB 311-318 except 312Director: Dr. Claudius Mundoma

[email protected]: www.sb.fsu.edu/core/pbf/pbf.htmlMulti-user Facility Facility training requiredSubscribe to “pbfusers” mail listing Nominal fee to use the Facility

Physical Biochemistry Facility (PBF)


Equipment, expertise, and trainingCircular Dichroism (CD)Isothermal Titration Calorimetry (ITC)Stopped & Quenched Flow systemsDynamic Light Scattering (DLS)UV-Vis & Fluorescence spectrometersAnalytical Ultracentrifuge (AUC)

Core: Protein Expression Facility (PEF)


KLB 312 A-GDirector: Dr. Joan Hare

[email protected]:www.sb.fsu.edu/core/pef/pef.htmlFacility training requiredFSU Bio Safety training requiredMulti-user self-contained FacilityPortions BSL2 CapableSubscribe to “pefusers” mail listingNominal fee to use the Facility

Protein Expression Facility (PEF)


Large scale production of proteins from mammalian and insect cells

Equipment, space and expertise provided for protein expression

Graduate student trainingAssistance for protein expression in E.

Coli - Proper bacterial handlingBio-Safety issues

Core: X-Ray Facility (XRF)


KLB 410-413Director: Dr. T. “Soma” sundaram

[email protected] page: www.sb.fsu.edu/~xray/Facility training requiredFSU Radiation Safety training requiredMulti-user FacilitySubscribe to “xrayusers” mail listingNominal fee to use the Facility

X-Ray Facility (XRF)


Macromolecular x-ray diffractionCrystal set-up and documentationData collection and processing3-D stereo viewing and interpretationCrystal storage and transportationSynchrotron scheduling and data

collectionIMB safety co-coordinatorIMB web site and computer issues

E-mail


E-mail and lists maintained by FSU ITSEveryone should subscribe to “imbpeople”https://lists.fsu.edu/mailman/listinfo/imbpeople

FSU official e-mail address preferred since important information may be sent

Part of registration or employment Core Facility users should subscribe to relevant lists Don’t send inappropriate materials Don’t respond to phishing or spam e-mails Nobody should ask your password If in doubt come and talk to one of the computer folks

Web site


IMB official web site maintained locally http://www.sb.fsu.edu

Individual web pageUser is responsible for making and maintaingDon’t post inappropriate materialsDon’t download copyrighted materialsDon’t host any P2P services

It is illegal and therefore don’t do itCompanies track usage and will sueAt minimum loose all access and previliges

Computer quota


We allocate quota for home directoryDon’t fill your home dir (Don’t exceed the

quota)Linux: /home/user_name (150 MB | 200 MB)Windows: c:\Documents and Settings\user_name (30 MB)(change soon)

Request special directory for dataLinux: Varies and provided by PIsWindows: d:\ or e:\

Archive and remove old data using DVD or tape drives

Computer Related


Only secure connections are allowed (ssh) Consult http://wiki.sb.fsu.edu Use virus scan before using removable media inside

IMB Personal laptops need latest anti-virus software before

usage (user should buy it!) Don’t connect your laptop without getting permission

from Michael Zawrotny Don’t assign your own IP, M. Zawrotny will do Violators will lose the computer privileges Assigned IP is portable within IMB (DHCP) Wireless is available throughout the building Wireless: FSUSecure better than FSUWin Printers are for business use only! Don’t print books or copyrighted materials!

Computer: Profile Size Errors

Reduce cache in IE & Firefox (~10 MB)

Don’t store files in Desktop Area

Store files in D:\ or E:\ drives

Remove or archive data often (3 months)

Use “Safely Remove Hardware” before unplugging flash drives


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Filled-up Profile Area

Almost filled Profile

Good Profile Size

Printer Related

Printers are for official use only!Color printer “cheetah”, KLB

4182nd color printer “scalpel”, KLB

317Poster printer PosterPS, KLB 418Remove or discard your print-

job!Don’t attempt to fix printers! Let

Rick, Soma, or Mike know


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Contact Info


Building Problems: Wade Baggett: 4-1419 (KLB 121) or Ed Kirkland: 5-1338 (KLB 123) orRick Wang: 5-3309 (KLB 119)

Bio/Chem Safety Issues:T.Soma sundaram: 4-6448 (KLB 414)Joan Hare: 4-1421 (KLB 304)

Computer Problems:Michael Zawrotny: 4-0069 (KLB 415)

T. Soma sundaram: 4-6448 (KLB 414)Rick Wang: 5-3309 (KLB 416)

IMB Fiscal/Admin


Purchase orderE-MarketPlace (VWR, GovConnection)Regular (Other vendors)Add new vendors to OMNI

Return receiving report to Debra/RosalindReport damaged goods immediatelyLog out-going FedEx and Fax

MOB/BioChem Students/Scholars


MOB CoordinatorDenise [email protected] | 4-1012

International Students/ScholarsMOB (Denise) | Others (Regina Ware)International Center (644-1702)

IMB Admin/Fiscal People


Regina Ware (4-4474 | KLB 118 | [email protected])Keys, Pay, Visas (Non-Student), & GrantsState & Markey Budgets

Patricia Brinkley (4-1209 | KLB 114 | [email protected])RF Grants & Travel

Rosalind Sapp (4-4764 | KLB 115 [email protected])Appointments and Purchase Orders

Denise Newsome (4-1012 | KLB 106 | [email protected] & Visas (Student)

Debra Walker-Fields (4-4764 | KLB 101 )Reception, Mail, Car Keys, & Seminar Room

Thanks


Feedbacks WelcomeThanks for Attending

Updates @www.sb.fsu.edu/~soma/Proj/Present/

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