20.109-Lecture-2011-03-15 - Amazon Web Services
Transcript of 20.109-Lecture-2011-03-15 - Amazon Web Services
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20.109Synthetic Biology Module
Lecture #3
Ron Weiss
Department of Biological Engineering
MIT
March 15, 2011Department of Biological Engineering
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DNA synthesis & genetic circuits
*Sampling of systems in publications with experimental circuits
207
2,100 2,7007,500 14,600
32,000
583,0001,080,000
100
1,000
10,000
100,000
1,000,000
1975 1985 1995 2005
Length in
base pa
irs
Year
DNA synthesis Synbio Pubs
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DNA synthesis & genetic circuits
*Sampling of systems in publications with experimental circuits
?
207
2,100 2,7007,500 14,600
32,000
583,0001,080,000
100
1,000
10,000
100,000
1,000,000
1975 1985 1995 2005
Length in
base pa
irs
Year
DNA synthesis Circuit size
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A collection of reusable parts
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System elements:Lambda repressor (cI)
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Bacteriophage
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Lysis/Lysogeny
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PR and PRM Promoters
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The Promoters
p. 44-45
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Repressor Positive and Negative Control
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Cooperative Binding