2 INTERNATIONAL CONFERENCE ON NATURAL...

University of Surabaya (UBAYA)

International Conference on Natural Resources and Life Sciences 2018 1

2ND INTERNATIONAL CONFERENCE ON

NATURAL RESOURCES

AND

LIFE SCIENCES

(NRLS 2018)

Managing Natural Resources for Better Health

Co-Organized by: Faculty of Biotechnology

Faculty of Pharmacy

Faculty of Medicine

University of Surabaya

IBIS STYLES HOTEL

JEMURSARI, SURABAYA

AUGUST 23-24, 2018


2 International Conference of Natural Resources and Life Sciences 2018

Contents

Contents ............................................................................................................ 2

Chief Organizer’s Welcoming Speech ............................................................ 3

Rector’s Opening Remarks ............................................................................. 5

NRLS 2018 Commitee ...................................................................................... 7

PROGRAM SCHEDULE .............................................................................. 10

ORAL PRESENTATION SESSION ............................................................. 12

POSTER SESSION ........................................................................................ 17

INVITED SPEAKERS ................................................................................... 20

Prof. Intan Ahmad .................................................................................. 21

Erhan Simsek, Ph.D. ................................................................................ 22

Assoc. Prof. Dr. Kanyaratt Supaibulwatana ............................................ 23

Prof. Dr. Varaporn Junyaprasert .............................................................. 24

Assoc.Prof. Dr.Heni Rachmawati ............................................................ 26

Dr. Fazren Azmi ...................................................................................... 27

Dr.rer.nat. Sulistyo Emantoko Dwi Putra ................................................ 28

Prof. Dr. Bob Wilffert ............................................................................. 29

ORAL ABSTRACTS ..................................................................................... 30

POSTER ABSTRACTS ................................................................................. 75

MISCELANEOUS ....................................................................................... 110

SPONSORS ................................................................................................... 117


International Conference of Natural Resources and Life Sciences 2016 3

Chief Organizer’s Welcoming Speech

The honorable Rector of UBAYA, Deans of UBAYA Faculties and faculty

members, our eminent invited speakers, sponsors, ladies and gentlemen, a very

good morning to you all!

As the times progressed, Biotechnology became evolved due to its wide scope

of knowledge, or more easily called multidisciplinary. Many areas of science

are biotechnology work areas, such as molecular biology, biochemistry,

environmental biology, genetics, forensic biology, immunology, microbiology,

pharmacology, botany, food technology and much more. It cannot be denied,

each of these scholars have its own peculiarities, causing some difficulty of

mixing up with each other. However, the combination of these disciplines is

anticipated in order to bring more benefits to the audience than if the field of

science is run independently.

Disruptive era is happening today, which is marked by a change in the existing

global business order. Many established businesses are eroded by many

businesses that are smaller because of the nature of sharing economy. Big retail

uprooted because online stores began scattered. Big hotel uprooted due to

business model like hospitality rental. It also may also have an impact on large

industries that utilize natural resources, such as Monsanto or other major

pharmaceutical industries. The use of natural resources whose information is

more opened will be more welcome than the disclosed research results. Just

imagine a science like Chinese traditional medicine, functional food, the use of

markers with technology that can be distributed cheaply will explode in the

market. This poses a challenge for scientists deepening the biological

disciplines and natural resources to contribute to the wider community through

their respective fields. The biotechnology community recognizes the need to

work with others, such as experts from various fields such as pharmacy and

medicine, to come up with ideas for health improvement efforts that can meet

the needs of society and not just the science that dwells in the ivory tower.

Therefore, the 2nd International Conference on Natural Resources and Life

Sciences – NRLS 2018 this time theme “Managing Natural Resources for

Better Health“.



Coincide with it, the NRLS 2018 event also will be hosted for the Konsorsium

Biologi Indonesia (KOBI) Congress-II and Ikatan Program Studi

Bioteknologi Indonesia (IPSBI) Summit. It is likely that this event opens

doors for collaborative opportunities for research and technology development

among academics and professionals. Moreover, with the involvement of invited

speakers from abroad, exchanges and allegedly research ideas with global

trending can also occur.

On this occasion also, I want to inform the good news for all the colleagues,

that Ubaya Biotech Store (UBS) has been initiated. UBS is a service center for

biotechnology services and products which is a downstream part of research in

the UBAYA Faculty of Biotechnology. Some products and services are still in

the development stage, but to date, the item and service list is getting ready and

increasing a lot to be too wasted if it is used only in the internal faculty. Going

forward, we personally hope that will be a consortium of biotechnology

services and products in the future, where relevant parties can exchange ideas

in the development of biotechnology products and services and fulfill their

needs.

We hope you will enjoy your time with us and being encouraged for the

collaborations and friendship, scientific exchange, development of joint

interests and project that are of scientific and economic importance in order to

explore the natural resources and life sciences and their importance in the most

aspect of living, e.g. food and health, agriculture, sustainable environment and

renewable energy development.

Thank you for your time and have a nice day

Surabaya, August 23, 2018,

Johan Sukweenadhi, Ph.D.



Rector’s Opening Remarks

Good Morning,

The NRLS Committee, Distinguished Invited Speakers, Ladies and Gentlemen,

all friends from all overseas as well as from different places in Indonesia, It

gives me great pleasure to extend to you all a very warm welcome to the

University of Surabaya (UBAYA) Campus and to say how grateful we are that

you have accepted our invitation to participate in this international conference

held by the Faculty of Biotechnology, Faculty of Pharmacy and Faculty of

Medicine UBAYA. I am so pleased to see many colleagues, professors, friends

and families. We have here participants from seven countries, making our

conference a global friendships celebration at once.

I would like to address my compliments to all committee and academic

colleagues from UBAYA, who have been working a lot to prepare this event.

Let me also address my gratitude also to industrial partners and sponsors

participating in this conference. I also wish to specially welcome the

representatives of biology and biotechnology related associations and express

to you my deep appreciation for sharing this important moment.

After the implementation of 1st NRLS 2016 two years ago, this time the

Faculty of Biotechnology works together with Faculty of Pharmacy and Faculty

of Medicine, University of Surabaya in the implementation of second edition

NRLS 2018. It is a great pleasure to me, knowing that three different faculties

of Ubaya, can do such a wonderful collaboration to organize this scientific

event beneficial to all parties. For your information, it is not the first time these

three faculties work to collaborate with each other. Recently, at July 18th 2018,

we inaugurated the new Laboratory named Kalbe Ubaya Hanbang-Bio

Laboratory (abbreviated as KUH Laboratory), which was a collaboration

project with the Industry. I hope the committee will have the opportunity to

please you all taking a short visit to the KHU Lab at UBAYA Campus. All

these events are actually part of our programs in celebrating the UBAYA

50Years Anniversary in 2018.

I have been informed that the theme of the today conference is “Managing

Natural Resource for Better Health”. To my understanding, this is then the

Conference where you can meet colleagues not only from your own specialty

area, but you can also build connection to many colleagues from other fields for

the sake of complementing each other towards a better scientific and

technological achievements and applications in the area of Natural Resources

and Life Sciences.



I hope this conference provides you with a forum to exchange scientific ideas,

inspire new research, and new contacts for closer co-operation, so that we can,

together, envisage the future of a promising development of Natural Resources

and Life Sciences to human’s benefits. Please carry also with you the best

wishes of UBAYA for a productive and successful conference, and a pleasant

stay amongst you in Surabaya.

Finally, on behalf of UBAYA, I am very proud to declare that the 2018

International Conference NRLS-2018 is officially opened. Thank you.

Surabaya, August 23, 2018,

Prof. Ir. Joniarto Parung, MMBAT, Ph.D.



NRLS 2018 Commitee

STEERING COMMITTEE

Chairman : Nemuel Daniel Pah, S.T., M.Eng., Ph.D.

(Vice Rector of Academic Affairs – UBAYA)

Chief of Programme : Dr. rer. nat. Maria Goretti Marianti Purwanto

(Dean of Biotechnology Faculty – UBAYA)

ORGANIZING COMMITTEE

Chief of Executive : Johan Sukweenadhi, Ph.D.

Vice of Chief Executive : Dr.rer.nat. Sulistyo Emantoko D. P.

1. Treasure

Dr. Ir. Popy Hartatie Hardjo, M.Si.

Riyadotul Husnah, SP.

2. Programme Section

Fenny Irawati, S.Si, M.Si

Wina Dian Savitri, S.Si., M.Agr.

Ardhia Deasy Rosita Dewi, S.TP., M.Sc.

Nina Dewi Oktaviyanti, S.Farm., M.Farm., Apt.

3. Administration Section

Olly Wicaksono Pratamasari, S.E., M.MT.

Nurul Azizah, S.Si.

Dwi Epatarini, S.Sos.

4. Scientific Committee

Dr. Dra. Mariana Wahjudi, M.Si.

(Faculty of Biotechnology – University of Surabaya)

Dr.rer.nat. Sulistyo Emantoko D. P.


Dr.rer.nat. Maria Goretti Marianti Purwanto


Dr. Ir. Popy Hartatie Hardjo, M.Si.


Johan Sukweenadhi, Ph.D.


Prof. Ir. Lieke Riadi, Ph.D.

(Faculty of Engineering – University of Surabaya)

https://my.ubaya.ac.id/inc/ajax/aKaryawanProfilePage.php?npk=214031



Dr. Dra. R.R. Christina Avanti, M.Si., Apt.

(Faculty of Pharmacy– University of Surabaya)

Dr. Finna Setiawan, S.Farm., M.Si.

dr. Irwin, M.Epid., M.Med.Ed.

(Faculty of Medical– University of Surabaya)

dr. Risma Ikawaty, Ph.D.


dr. Nur Flora Nita Taruli Basa Sinaga, M.Kes., Sp.OT., M.H.


Prof. Intan Ahmad

(Ministry of Research, Technology and Higher Education,

Republic of Indonesia)

Prof. Dr. Bob Willfert

(University of Groningen, Netherland)

Prof. Dr. Varaporn Junyaprasert

(Mahidol University, Thailand)

Prof. Dr. Jagat R. Kanwar

(Deakin University, Australia)

Dr. Fazren Azmi

(Universiti Kebangsaan Malaysia, Malaysia)

Erhan Simsek, Ph.D.

(Agilent Technologies Singapore)

Assoc. Prof. Dr. Kanyaratt Supaibulwatana

(Mahidol University, Thailand)

Assoc.Prof. Dr. Heni Rachmawati

(School of Pharmacy, Bandung Institute of Technology)

5. Promotion and Documentation Section

Mangihot T. Gultom, M.Sc.

Ida Bagus Made Artadana, S.Si., M.Sc.

6. Venue, Acommodation and Equipment Section

Dr. Dra. Tjandra Pantjajani, M.S.

Ruth Chrisnasari, S.TP., M.P.

7. Transportation Section

Ida Bagus Made Artadana, S.Si., M.Sc.

Fenny Irawati, S.Si., M.Si.

8. Sponsorship Section

Ernest Suryadjaja, S.Si., M.App.Sc.








9. Workshop Section

Ruth Chrisnasari, S.TP.

Dr. Dra. Mariana Wahjudi, M.Si.

Dr.rer.nat. Sulistyo Emantoko D. P.

Akhmad Subhkan, S.Tp.

Fitria Setya Megawati, S.Si.

Ayu Mayzuhroh, S.TP.






PROGRAM SCHEDULE

DAY I Thursday, August 23, 2018

Plenary Session

07.30-08.00 Registration

08.00-08.15 Traditional Dance UBAYA Dance Team

Opening MC

Singing: Indonesia National Anthem Biotechnology student

08.15-08.40 Welcome speech I Rector

Welcome speech II Chief Organizer(s)

Opening ceremony (gong)

Keynotes Session I Moderator:

Dr. rer. nat. Sulistyo Emantoko

08.40-09.15 Revisiting the capacity of Indonesia’s

Higher Education in response to environment, energy and sustainability

challenges

Prof. Intan Ahmad

09.15-09.50 Solution to Discovery Metabolomics: High Resolution Mass Spectrometry with

Powerful Data Analysis Software

Erhan Simsek, Ph.D.

09.50-10.05 Discussion Moderator:

Dr. rer. nat. Sulistyo

Emantoko

10.05-10.20 Coffee break I

Keynotes Session II Moderator:

IBM Artadana, M.Sc.

10.20-10.55 Genetic interference and modified

environments affected on growth and

terpenoid variations of Artemisia annua L.

Assoc. Prof. Dr. Kanyaratt

Supaibulwatana

10.55-11.30 Role of Nanoparticles of Polymer-drug Conjugate for Effective Drug Delivery

Prof. Dr. Varaporn Junyaprasert


IBM Artadana, M.Sc.

11.45-12.00 Sponsor presentation I Sciencewerke

12.00-13.00 Lunch + Poster Session

Parallel Session

13.00-14.00 Parallel session I

14.00-14.15 Coffee break II

14.15-15.15 Parallel session II



DAY II Friday, August 24, 2018

Plenary Session

07.30-08.00 Registration

08.00-08.05 Opening MC

08.05-08.20 Traditional Dance Pharmacy students

Keynotes Session I Moderator:

Dr. Mariana Wahjudi

08.20-08.55 (Title) Prof. Dr. Jagat R. Kanwar

08.55-09.30 The pro-apoptotic effect of curcumin

nanoemulsion versus curcumin solution in

MCF-7 and MDA-MD cells in vitro

Assoc. Prof. Dr. Heni

Rachmawati


Dr. Mariana Wahjudi

09.45-10.00 Coffee break I

10.00-10.10 Welcome speech Vice Rector

10.10-10.20 MoU Signing between UBAYA and other Biology/ Biotechnology Associations

Keynotes Session II Moderator:

Dr. Dra. R.R. Christina Avanti,

M.Si., Apt.

10.20-10.55 Recent Advances in Nanotechnology-based

Vaccines

Dr. Fazren Azmi

10.55-11.30 Placental Global and Specific DNA

Methylation: A Mirror of Pregnancy and

Birth Outcome Quality

Dr. rer. nat. Sulistyo Emantoko



M.Si., Apt.

11.45-12.00 Sponsor presentation II Biotek Prima Indoplus

12.00-13.00 Lunch + Poster Session

Parallel Session

13.00-14.00 Parallel Session I

Keynotes Session III Moderator:


M.Si., Apt.

14.00-14.35 Implementation of pharmacogenetics in

clinical practice

Prof. Dr. Bob Willfert



M.Si., Apt.

14.45-15.00 Coffee break II

Parallel Session

15.00-16.00 Parallel session II

15.00-16.15 Announcement of Best Poster and Best Oral

Presenter

MC

16.15-16.30 Closing MC



ORAL PRESENTATION SESSION

DAY I Thursday, August 23, 2018

MEDICAL AND FORENSICS – BR LINE ROOM (OP-A-1 to OP-A-

6)

CODE TITLE PRESENTER MODERATOR

OP-A-1

Quantification of Expression of

Glucocorticoid Receptor of the

Offspring of Mus musculus

BALB/c of Stressed Parents: Folic

Acid Intervention Study

Shintya

Tanggara

Mariana

Wahyudi OP-A-2

Understanding the Current Event

of Tuberculosis in Indonesia :

Challenges and Opportunities

Risma

Ikawaty

OP-A-3

Anticancer Potential from Ethanol

Extract of Zanthoxylum

acanthopodium Seed to Against

MCF-7 Cell Line

Emilia Vivi

Arsita

DISCUSSION

Sulistyo

Emantoko

COFFEE BREAK

OP-A-4

Biological Screening of Leaf and

Fruit Extract (Ficuscarica L)

using Brine Shrimp Lethality Test

(BSLT) Toxicity Bioassay on

Arthemia salina Leach Larvae

Risa

Purnamasari

OP-A-5

Designing of a Less-

Immunogenic Nattokinase from

Bacillus subtilis subsp. natto: A

Computational Mutagenesis

Yoanes Maria

Vianney

OP-A-6

Alpha Mangostin Effect on MDA

Level and The Pancreatic

Morphology Rattus novergicus

Induced Alloxan

Devyana Dyah

Wulandari

DISCUSSION



AGRICULTURE – BR STRIPES ROOM (OP-B-1 to OP-B-10)


OP-B-1

Plant Regeneration Through

Somatic Embryogenesis from

Callus of Vanda tricolor

Lindl. var. pallid

Popy Hartatie

Hardjo

Johan

Sukweenadhi

OP-B-2

Influence of Types and

Concentration of Natural

Pesticides to Mortality of

Red Mite (Panonychus citri

McGregor)

Dyah

Roeswitawati

OP-B-3

Effect of Salinity on Biomass

Allocation and Whole Plant

Mass of Rice (Oryza sativa

L) Related to Growth at

Panicle Initiation and

Heading Stages

Niki Habibi

OP-B-4

Study of Ccs and pAMT

Gene of Genotype 1 Cayenne

Pepper (Capsicum frutescens

L.) Original Type and Mutant

Nur

Rahmattullah

OP-B-5 Menthol from Stem and Leaf

in vitro Mentha piperita

Bintang

Kerta Wijaya

DISCUSSION

COFFEE BREAK

OP-B-6

Analysis of Abscisic Acid,

Salicylic Acid and GABA in

Several Oil Palm Progenies

in Drought Stress

Susy Saadah

Popy Hartatie

Hardjo

OP-B-7

Uptake of Macro Nutrients

and Growth of Mahang

plants (Macaranga gigantea) Dwi Susanto

OP-B-8

The Evaluation of Breeding

Value and Most Probable

Producing Ability for the

Basis Selection of Ettawa

Croosbred Goat at Malang

Region, East Java, Indonesia

Aris Winaya

OP-B-9

InVitro Multiplication Of Fig

(Ficus carica L.) for

Commercially Industry

Ivon Grace Paulin

OP-B-10

Mild Alkaline Pretreatment

on Sugarcane Bagasse:

Effects of Time and Lime

Ratio to Dry Bagasse

Lieke Riadi

DISCUSSION



FOOD AND NUTRITION – BR CURVE ROOM (OP-D-1 to OP-D-10)


OP-D-1

Effect of Phosphoric Acid

Pretreatment on Characterization

of Gelatin from Broiler Chicken

Bones

Dewi Yuliani

Sulistyo

Emantoko

OP-D-2

Molecular Characterization of

Potentially Probiotic Bacterium

Isolate from Mackerel Fish

(Rastrelliger sp.)

Yulia Sari

Ismail

OP-D-3

Profilling Of Shrimp Shell

Hydrolysis Product By Chitinase

From Bacillus licheniformis B2 Maria Goretti

OP-D-4

Production, Purification and

Characterization of Chitinase

from Bacillus licheniformis D11

Adelia Angelin

Anastasia

OP-D-5

Purification and Characterization

Of Chitinase from Paenibacillus

sp.

Maria Fidelia

Susanto

DISCUSSION

COFFEE BREAK

Ardhia Deasy

R.D.

OP-D-6

Rapid Reversed-Phase High

Performance Liquid

Chromatography Assay of tert-

Butylhydroquinone content in

Food Products

Steven Sutanto

OP-D-7

Characterization of

Thermostable Chitinase from

Bacillus licheniformis B2 Nalista Jayanthi

OP-D-8

The Isolation and Screening of

Proteolytic Bacteria from

Sidoarjo Shrimp Paste as

Protease Source to Extract the

Collagen Protein of Milkfish

Scales

Evi Susanti

OP-D-9

A New Specific Primer for

Detection of Pig genome by

Quantitative Polymerase Chain

Reaction

Mariana

Wahjudi

OP-D-10

The Method of Growing Tubular

Acetobacter xylinum on the

Solid Media as Precursor of

Sausage Casing

Salafudin

DISCUSSION



DAY II Friday, 24August 2018

MEDICAL AND FORENSICS – BR STRIPES ROOM (OP-A-7 to

OP-A-14)


OP-A-7

In Vivo Efficiency of Collagen

Extract from Sangkuriang

Catfish (Clarias gariepinus var.

Sangkuriang) Skin on MDA

Levels and Macrophages Total

in Burn Wound Healing

Andini Ary

Mariana

Wahyudi

OP-A-8

The Effect Of Folic Acid

Supplementation In Parental

Stress Mouse Toward NR3C1

Expression Alteration In The

Offspring

Patricia

OP-A-9

In Silico Mutagenesis:

Decreasing the

Immunogenicity of Botulinum

toxin Type A

Stanley Evander

Emeltan Tjoa

OP-A-10

Comparison of food intake

containing vitamin D with BMI

of Geriatric patients in

Sidoarjo, East Java

Rivan Virlando

Suryadinata

DISCUSSION

Sulistyo

Emantoko

COFFEE BREAK

OP-A-11

Influenceof Folic Acid Diet on

Maternal Stress to Offspring

Stress Level and NR3C1

Expression

Natanya

OP-A-12

Facile Green Synthesis of

Silver Nanoparticles from

Ethanol Extract of Plantago

major L. and its antibacterial

activity

Sukweenadhi

Johan

OP-A-13

In Vitro Evaluation of

Chitosan-DNA Plasmid

Complex Encoding Jembrana

Disease Virus Tat Protein as a

Vaccine Candidate

Asmarani

Kusumawati

OP-A-14

The Relationship between

Asthma Control -

Methylxanthine used and

cyp1a2*1f Gene

Polymorphisms

Mariana Wahjudi



DISCUSSION

ENVIRONMENT & ENERGY(1) – BR CURVE ROOM (OP-C-1

to OP-C-6)


OP-C-1

Utilization Cytochrome B Gene

for Phylogeny Analysis of

Cuora amboinensis to Prevent

Extinction: Preliminary Study

Dwi Martha

Nur Aditya

Mangihot Tua

Goeltom OP-C-2

Profile of Antibiotic

Compounds

fromTrichodermaharzianumin

Controlling Aspergillus

flavusonBaliLontar

IBG Darmayasa

OP-C-3

Plasmid Curing Analysis of

Chromate Reducing Bacteria

Isolated from Serpentine Soil Badruzsaufari

DISCUSSION

Finna

COFFEE BREAK

OP-C-4

Effect of Cycle Time on

Biokinetics Parameters in

Tapioca Wastewater Treatment

using Sequencing Batch

Reactor

Happy Mulyani

OP-C-5

The Utilization of Tofu Waste

for the Cultivation of Spirulina

for Biomass Production Yalun Arifin

OP-C-6

Thermostable Chitinase-

Producing Bacterium from Ijen

Hot Spring – Indonesia:

Isolation, Identification and

Characterization

Ruth

Chrisnasari

DISCUSSION



POSTER SESSION SCHEDULE

Thursday – Friday, 23 – 24 August 2018

Prefunction Area

MEDICAL AND FORENSICS (PP-A-1 to PP-A-4)

CODE TITLE PRESENTER

PP-A-1 Antibacterial Activity Roll on Deodorant With

Plucheaindica (L.) Leaf Extract Against

Staphylococcus epidermidis in-Vitro

Oom Komala

PP-A-2 Formulation of Padinaaustralis Extract Tablet as

Antibacterial Escherichia coli Tri Saptari

Haryani

PP-A-3 The Potential of Pacitan Orange (Citrus sinensis)

Peel Extract as a Stimulus of Regeneration Cells in

Rattus norvegicus Burns

Rinza

Rahmawati

Samsudin

PP-A-4 Characterization and Antibacterial Activity Test of

Simbion Sponge Bacteria Against Pathogenic

Bacteria Endang

AGRICULTURE (PP-B-1 to PP-B-5)


PP-B-1 Genetic Variability of Soybean Genotypes for Pod

Shattering Resistance using Oven Methods Ayda

Krisnawati

PP-B-2 Characterization of Adaptive and Productive

Soybean Genotypes in Dry Land of Kalimantan,

Indonesia

M Muchlish

Adie

PP-B-3 The Performance of Three Local Rice (Oryza sativa)

Cultivar from East Kalimantan under Osmotic Stress

at Early Seedling Stage

Ida Bagus Made

Artadana

PP-B-4 Production Potential of Sweet Corn (Zea mays Linn.

Var. 17accharate) to Different Planting Pattern and P

Sources

P G Adi Nurani

PP-B-5 Local Variety of “Uwi” (Dioscorea alata Linn.)

Plant in West Region of East Java Upland Wuryanto



ENVIRONMENT & ENERGY (PP-C-1 to PP-C-7)


PP-C-1 Phytotoxicity and Developmental Effect of

CASHEW Nut Shell Extract against Nymphal

Instar of Whitefly (Bemisia tabaci Genn.)

Wuye Ria

Andayanie

PP-C-2 Applying Species Distribution Models to

Identify the Conservation Area by Area Habitat

Suitability: A case study using Zanthoxyluma

canthopodium DC.

Cicik Suriani

PP-C-3 An Engineering of Floating Net Aquaculture

System of Tiger Grouper [Ephinephelus

fuscoguttatus (Forsskal, 1775)] on

suplementation Bacillus subtilis (Ehrenberg,

1835) Probiotic in the Diet

D Rachmawati

PP-C-4 Performance UNEJ #1 Stove Low Energy using

Bio-pellet from Coffee Husk S S Harsono

PP-C-5 Fish Diversity in Cimanuk River Before and

After Rainy Season in Jatigede reservoir, West

Java, Indonesia

T Herawati

PP-C-6 The Fe Non-heme Prospect in Coffee Flour made

from Coffee Solid Waste: Mini review RHSetiabudi

PP-C-7 Does the utilization of waste as the green energy

alternative economically feasible? N Syaifudin

FOOD AND NUTRITION (PP-D-1 to PP-D-10)


PP-D-1

Preparation and Characterization of Butterfly Pea

Color Extract Encapsulated with Various

Coating Agents

Hana Susanti

Maleta

PP-D-2 Photodynamic Inactivation of Escherichia coli

by the Crude Pigments Extract of Various Tea

Dregs

Renny Indrawati

PP-D-3 The Function of Ethanolic Extract Plectranthus

ambioinicus Lour Spreng as Preventive and

Curative Against The Effects of Rhodamine B in

Rats

Melva Silitonga

PP-D-4 Potency of Vegetable Oil to Increase Microflora Nurhayati



Health Using In Vitro and In Vivo Evaluation

PP-D-5 Quality and Consumer Acceptance Improvement

on Prime Local Soy Milk (Glycine max (L)

Merrill) Varieties

Elfi Anis Saati

PP-D-6

The Utilization of Telang Flower as Healthy-

Natural Food Coloring on Dawet Drink Elfi Anis Saati

PP-D-7 Artificial Rice as an Alternative Functional Food

to Support Food Diversification Program Erica Valencia

PP-D-8 Critical Aspects to Produce Low-Cost Protein

Molecular Weight Marker Leonardo Tejo

Gunawan

PP-D-9 Potency healthy Moringa oleifera (Lamk) leaf

flour as home-agroindustry in rural area of

Eastren East Java - Indonesia

S.S Harsono

PP-D-10 Quality of Milk Pasteurization of Ginger in Cold

Strorage KK Khotimah



INVITED SPEAKERS



Revisiting the capacity of Indonesia’s Higher Education in response

to environment, energy and sustainability challenges

Intan Ahmad

Ministry of Research, Technology and Higher Education, Republic of Indonesia

[email protected], [email protected]

The 21st century has witnessed increasing environmental, energy and

sustainability challenges in our lives that need to be addressed by our Higher

Education system. On one hand, Indonesia’s current predicaments in fulfilling

Sustainability Development Goals (SDGs) have invited international scrutiny,

particularly with the rapid depletion of Indonesia’s natural resources. On the

other hand, whilst many Indonesian universities have managed to put these

challenges in their agenda and produced relevant innovations and research to

solve the recurring problems, we are still lagging behind compared to other

countries in Asia, both in terms of publications and intellectual property rights

related to environment and energy. This speech highlights some of the

strategies we need to look into to address environment, energy and

sustainability challenges and stand equally with international scholars in

providing globally-oriented solutions and insights. This, as I argue, can be

achieved by revisiting the potentials, roles and responsibilities of Indonesia’s

Higher Education system.

mailto:[email protected]



Solution to Discovery Metabolomics: High Resolution Mass

Spectrometry with Powerful Data Analysis Software

Erhan Simsek

Agilent Technologies Singapore

[email protected]

The field of metabolomics has seen tremendous growth in recent years with

important applications in basic and life sciences. Mass spectrometry coupled to

chromatographic separation techniques is one of the major analytical platforms

to analyze metabolites in complex biological samples due to its highly sensitive

and fast data acquisition capabilities. This presentation will cover workflows

and instrumentation for discovery as well as targeted metabolomics approaches.

Data analysis steps such as feature extraction, statistical analysis, metabolite

identification and pathway analysis will be covered with relevant software tools

such as Profinder, Mass Profiler Professional and Pathway Architect.



Genetic Interference and Modified Environments Affected on

Growth and Terpenoid Variations of Artemisia annua L.

Kanyaratt Supaibulwatana

Department of Biotechnology, Faculty of Science, Mahidol University,

Bangkok, Thailand

[email protected], +66 (0)2 2201 5303

Abstract

Plant secondary metabolites are the final products of regulatory processes and

the level of metabolite can be influenced depending on genetic or

environmental changes. Among plant secondary metabolites, terpenoids are a

most structural diverse group formed from 5-C subunits, isoprene, synthesized

from movalonate and 1-deoxy-D-xylulose-5-phosphate (DOXP) pathway. In

recent years, incorporation of biotechnology with the advance technologies of

analytical biochemistry and high-throughput omics led to more increasing

numbers of complete plant genome sequences and better understanding in

functional genomic study and more importantly, their role as a basis for drug

development. Besides the genetic interferences by metabolic bioengineering,

induced mutation are also useful to establish new mutants that may be

implemented for production of high-yielding of high-value phytochemical. In

this presentation, genetic interferences incorporated with modified

environmental controlled environments with artificial lights that had effects on

morphological growth and phytochemical changes will be demonstrated using a

medicinal herb, Artemisia annua L. as a model plant. A. annua L., a medicinal

herb belongs to family Asteraceae. It is so-called as “Qinghao” in Chinese that

has been the used as anti-fever and anti-malarial drugs for more than thousand

years. The phytochemical investigation of this plant revealed that a

sesquiterpene lactone compound named artemisinin has been predominantly

detected in the aerial parts of this plant, particular in the leaves. The variations

of plant growth, terpenoids production and its bioactivity affected by genetic

manipulation and external stimulators will be discussed.



Role of Nanoparticles of Polymer-drug Conjugate for Effective

Drug Delivery

Varaporn Junyaprasert

Department of Pharmacy, Faculty of Pharmacy, 2Center of Excellence in

Innovative Drug Delivery and

Nanomedicine, Faculty of Pharmacy, Mahidol University, Bangkok 10400,

Thailand

[email protected]

One of the most useful techniques to modify and control drug release or to

achieve targeted delivery is to use polymer-drug conjugated nanoparticles.

Polymer-drug conjugates are technologies in which a drug is covalently bound

to a polymeric carrier, normally via a biodegradable linker. The main benefits

of polymer-drug conjugates are improvement of properties of drug carriers,

leading to polymer carriers with high loading capacity and enhanced cellular

uptake. In addition, grafting the drug onto the polymer backbone can modify

the drug release and reduce burst drug release. Targeted delivery is generally

required for toxic drug substances e.g. anticancer and antineoplastic drugs so

as to enhance the specific delivery to a particular organ for maximum effect,

thereby reduce or eliminate unwanted side effects to healthy tissues. The

polymer-drug conjugated based nanoparticles possess ability to deliver the

drug to targeted cells or organs by passive targeting via the enhanced

permeability and retention (EPR)effect and active targeting by functionalizing

targeting ligands onto the nanoparticle surface (2-3). Therefore, the polymer-

drug conjugates have demonstrated favorable physicochemical properties to

enhance drugdelivery efficiencyIn this lecture, two studies of polymer-drug

conjugates have been demonstrated to use as drug targetingand delivery. The

first study is “Enhanced toxicity and cellular uptake of methotrexate (MTX)-

conjugatednanoparticles in cancer cells by decorating with folic acid-

conjugated d-α-tocopheryl polyethylene glycol1000 succinate (TPGS-FOL)”

(4). The second one refers to “Cationized triblock and diblock poly( -

caprolactone)-copoly(ethylene glycol) (PCL-PEG) copolymers for oral

delivery of enoxaparin (ENX)” (5-6). In the first study, the results confirmed

the successful synthesis of TPGS-FOL. In addition, the decoration of TPGS-

FOL onto the MTX nanoparticles slightly enlarged the size and enhanced

cytotoxicity and uptake efficiency to MCF-7. Furthermore, 10% MTX-

conjugated nanoparticles and FOL-decorated nanoparticles showed higher

%uptake, as compared to 20% MTX-conjugated nanoparticles and

undecorated



nanoparticles. For the cationized PCL-PEG copolymers for ENX delivery, the

study showed that the content of PTA on P(CL) chain had a large impact on

the copolymer and particle properties, the cytotoxicityof Caco-2 cells, drug

encapsulation, and release behavior. However, the different conformations of

the

ENX-P(CatCLCL)2-PEG and ENX-P(CatCLCL)-mPEG particles did not

affect the particle properties, cellular uptake, and permeation. These results

suggested that these novel cationized copolymers would be potential carriers

for oral delivery of ENX.



The Pro-Apoptotic Effect of Curcumin Nanoemulsion versus

Curcumin Solution in MCF-7 and MDA-MD Cells In Vitro

Heni Rachmawati1,2, Najwa Nabila1, Christian1, Yogi Handoko1, Sabina

Quarder3, Kazunori Kataoka3

1School of Pharmacy, Bandung Institute of Technology, Ganesha 10 Bandung

40132, Indonesia 2Research Center for Nanosciences and Nanotechnology, Bandung Institute of

Technology, Ganesha 10 Bandung 40132, Indonesia 3Innovation Center of NanoMedicine, 3-25-14 Tono-machi, Kawasaki-ku,

Kawasaki City, Kanagawa Prefecture, Japan

Heni Rachmawati ([email protected])

Curcumin, a main and important active compound present in Curcuma sp.,

demonstrates a wide range of positive biological effects including for cancer.

However, due to low bioavailability caused by low solubility and stability as

well as intensive hepatic metabolism, the clinical outcome during curcumin

therapy is unsatisfied. Hence, we established nanocarrier system to improve the

lack of curcumin which was confirmed to be successful for our various studies.

In this report, we describe the potential use of curcumin loaded nanoemulsion

for anticancer. We performed uptake study of curcumin-loaded nanoemulsion

compared with curcumin solution in various cancerous cell models including

MCF-7, MDA-MB 231, Tov-21, and HepG2 cells. Among cell models tested

for in vitro study, MCF-7 cell showed the most sensitive cell type to curcumin

indicated by lowest IC50 value. Negative charge of curcumin-loaded

nanoemulsion was failure to cross the cell membrane of all cell lines. However,

slightly positive charge of curcumin-loaded nanoemulsion exhibited better

penetration to reach the cell nuclei shown by the green spots surrounding the

cell nuclei, although less as compared to curcumin solution. The capability of

curcumin to cross the cell membrane was followed by the apoptotic effect on

both MCF-7 and MDA-MB 231 cells, which was concentration dependent.

According to this finding, we suggest one of anticancer activity of curcumin is

through apoptotic pathway which is also reported by other groups. Our

established nanocarrier system might improve this effect via enhancing the

pharmaceutic properties of the curcumin.



Recent Advances in Nanotechnology-based Peptide Vaccines

Fazren Azmi

Faculty of Pharmacy, National University of Malaysia

[email protected]

Vaccination has emerged as the most effective and economically viable

medical discovery to improve public health. Majority of the conventional

vaccines were produced using live or attenuated microorganisms or parts of

them. However, the use of whole organisms or large proteins has several

weaknesses, such as the presence of biological impurities and unnecessary

epitopes which may contribute to reactogenic responses. Peptide vaccines offer

an alternative strategy as it contains specific epitope of an antigen, thus

eliminate the allergenic/reactogenic sequences. Interestingly, the use of

peptides as antigen open a new concept in vaccination, where there is a

renewed interest in developing therapeutic vaccine for certain chronic diseases,

such as cancer. However, peptide antigens produce a relatively weak immune

response which necessitate the need of immunostimulants (adjuvants) in

vaccine formulation for optimal efficacy. The use of nanoparticles in peptide

vaccine formulations can boost the antigen effectiveness and can be

functionalised for a targeted delivery. Various types of nanoparticles such as

virus-like particles, liposomes and polymeric nanospheres have been developed

as potential delivery vehicles for peptide-based vaccine antigens. Recent

approach in utilising theself-assembling peptides as a nanocarrier platform for

peptide vaccines will be discussed in detail.




Placental Global and Specific DNA Methylation:

A Mirror of Pregnancy and Birth Outcome Quality

Sulistyo Emantoko

Department of Biology, Faculty of Biotechnology, University of Surabaya

Jalan Raya Kalirungkut Surabaya 60292, East Java Province, Indonesia

[email protected]

Fetal programming refers to epigenetic adaptative measures to adverse prenatal

environments which affect the birth outcome and disease susceptibility in later

life. Amongst other epigenetic mechanisms, DNA methylation plays an

important role in fetal programming. DNA methylation dynamics during

embryogenesis, development, and baby born are important windows in the

mammalian life cycle. DNA methylation is possible to measure at a global level

as represent the amount of 5mC in the genome or at a specific level of the

certain gene. Placenta as important organ which developed during this time

period, experience the same DNA methylation dynamics. At the end of this

process, a specific DNA methylation pattern is figured out as a response to its

surroundings. A complex interaction between mother, placenta, and child

ensures an ideal supply of nutrients, oxygen and endocrine signals, all

necessary requirements for normal development. This prenatal environment

strongly depends on maternal health and placental function. Maternal

pregnancy-related diseases like gestational diabetes (GDM) are known to

disturb placental function and the prenatal environment. As such, maternal

diseases can impact on birth outcomes and according to the theory of fetal

programming, increase the risk for disease in later life of the newborn.



Implementation of Pharmacogenetics in Clinical Practice

Prof. Dr. Bob Wilffert

Department of Pharmaco Therapy, -Epidemiology and –Economics,

Faculty of Science and Engineering, University of Groningen, Netherland

[email protected]

Pharmacogenetics is one source for explaining inter individual differences in

drug response, besides factors like age, ethnicity, organ dysfunction, disease,

pregnancy, gender, etc. The value of the application of pharmacogenetics in

pharmacokinetics (as example: CYP2D6, CYP2C19, CYP2C9),

pharmacodynamics (as example: VKORC1) and side effects (as example:

HLA-B*5107) will be described. The effect of pharmacogenetic variation in

CYP2D6 on the bioavailability of oral nortriptyline will be compared with the

requirements for bioequivalence. There are big, drug specific differences in the

role of pharmacogenetics and its clinical impact. The role of ethnicity in

pharmacogenetics will also be illustrated. The initiative of the

Pharmacogenetics Working Group of the Royal Dutch Pharmacists Association

taken in 2005 to facilitate implementation of pharmacogenetics in clinical

practice will be presented. This working group consists of (clinical)

pharmacists, physicians, clinical chemists, epidemiologists and clinical

pharmacologists. Literature is searched for pharmacogenetic information. The

way of assessing level of evidence and of defining the clinical implications will

be explained, as well as the translation into clinical practice. A scoring system

for the necessity of preemptive genotyping will be presented. The advices

formulated by the working group are also taken up on the website from

PharmGkb. Pharmacogenetics can be applied to select the most appropriate

drug for the individual patient and should be applied more in clinical practice.



ORAL PRESENTER



MEDICAL & FORENSICS



OP-A-1

QUANTIFICATION OF GLUCOCORTICOID RECEPTOR

EXPRESSION IN Mus musculus BALB/c OPFFSPRING OF

STRESSED PARENTS: FOLIC ACID INTERVENTION STUDY

Shintya Tanggara, Sulistyo Emantoko Dwi Putra, Ida Bagus Made Artadana

Faculty of Biotechnology, University of Surabaya, Kalirungkut Road, 60292,

Surabaya, East Java, Indonesia

*Corresponding author: [email protected]

Abstract

HPA axis plays a crucial role in regulating stress mechanism and is made up of

complex system in support of that. One of the components that plays a major in

stress management is the glucocorticoid receptor (GR) expressed throughout

several areas of the body and especially hippocampus. GR is responsible in

regulating cortisol level in the body and is encoded by the gene NR3C1.

Previous studies shown the plasticity of hippocampus along with NR3C1’s

susceptibility to epigenetic changes. The possiblility of chronic restraint stress

(CRS) on both male and female BALB/c mice to induce epigenetic

reprogramming is evaluated using forced swim test (FST) as behavioural test

along with intensity ratio of NR3C1:GAPDH as molecular test. Effect of folic

acid on both parents was also investigated as it has been shown to have

antidepressant propertie and can normalize epiegenetic patterns in previous

studies. Behavioural test show for parents show that there is a significant

difference in the result, which is p=0.028 of CRS induced parents, offspring of

CRS induced parents (p=0.014), and normal parents. There is no significant

difference on behavioral test for folic acid-CRS (p=0.071) and folic acid-

normal (p=0.455) parents and for offspring as well (p=0.868 and p=0.890

respectively). Molecular result of the experiment was done on offspring and

showed significant difference on offpring with stressed parents (p=0.043) and

no significant difference on normal-folate offspring (p=0.833) and stress-folic

acid offspring (p=0.631).

Keywords: BALB/c mice, chronic restraint stress (CRS), epigenetics, forced

swim test (FST), gene expression quantification, glucocorticoid receptor (GR),

HPA axis, NR3C1

mailto:Corresponding%20author:%[email protected]



OP-A-2

UNDERSTANDING THE CURRENT EVENT OF

TUBERCULOSIS IN INDONESIA: Challenges and Opportunities

Risma Ikawaty, M.D., Ph.D.

Medical Faculty, University of Surabaya, Surabaya, East Java, Indonesia


Abstract

Multi-drug resistant Mycobacterium tuberculosis (MDR-TB) is a growing

international health problem, and it is mostly concentrated in developing

countries. In 2016, the World Health Organization reported that Indonesia

became the second largest contributor of global tuberculosis (TB) just after

India. The estimated incidence of TB burden was 1.020.000 of TB with HIV

and 32.000 of MDR/RR-TB (MDR is TB resistant to rifampicin and isoniazid,

while RR is rifampicin resistant). Increasing prevalence of MDR-TB strains

combined with HIV co-infection contributed greatly to the difficulties of TB

control and treatment. Epidemiological evidence of the rising MDR-TB threat

including mortality rate of 42/100.000 population, total new case and relapse

around 364.671, and TB treatment coverage was about 36% among 261 million

population in Indonesia. In this study we will review a comprehensive aspect of

epidemiologic triangle of MDR-TB in Indonesia. As we know that long-life

battle between MTB and the human host is determined by complex of host-

pathogen-environmental factors. Rifampicin as the first line anti-TB drug is

metabolized through hepatic cytochrome P450 enzyme system.

Pharmacogenomics analysis ofCYP450 genes provides the information whether

the patients are poor-, intermediate-, extensive-, or ultra-rapid metabolizer,

which further shows significant variability in drug responses including optimal

dose,efficacy and adverse drug reactions.At the same time, an extensive whole-

genome studies reveal various molecular targets related to resistance

mechanisms in MDR-TB(i.e.rpoB, rpoA, rpoC, gyrA, gyrB, etc) including

cross-resistance, resistance pathways, and yet the spontaneous mutations in

drug targets ensure the survival of M. tuberculosis.Environment factors also

play an important role to the TB treatment success rate. Several contributing

factors such as tobacco use (i.e. smoking), nutritional status, poor patient-

medication adherence, poor drug quality/supply, socio- economic status, etc.

These overall facts has made the approach and treatment of tuberculosis

become even more challenging, and further the development of novel and

efficient therapeutic substances is needed.




Keywords: Multi-drug resistant Mycobacterium tuberculosis, epidemiologic

triangle, pharmacogenomics, whole-genome study



OP-A-3

Anticancer Potential from Ethanol Extract of Zanthoxylum

acanthopodium Seed to Against MCF-7 Cell Line

Emilia Vivi Arsita*1, Dora Erawati Saragih1, Kelvin Aldrin2

1 Padjadjaran University, Faculty of Math and Science, Bandung/Sumedang

Road, West Java 45363, Indonesia

2 Padjadjaran University, Faculty of Pharmacy, Bandung/Sumedang Road,

West Java 45363, Indonesia


Abstract

Andaliman (Zanthoxyluma canthopodium DC) is a wild plant species typical of

North Sumatera that often used as spice for Batak cuisine. Studies conducted by

Wijaya (1999) show that Andaliman has potential as anticancer drug. Based on

statistic data, breast cancer is type of cancer that most common in women. Cell

MCF-7 is one of breast cancer model. The purpose of this research was to

identified types of secondary metabolites which found in andaliman’s seed

extract and to test the ability of these compound in stopping the MCF-7 cell

activity. The method in this research is extraction use ethanol with different

concentration and MTT Assay. Identified secondary metabolites such as

phenol, saponins, flavonoids, tannins, terpenes, steroids, and alkaloids. IC50

MTT Assay value with absorbance of 550 nm was 221.31 ppm. IC50 values

indicate that the andaliman seed ethanol extract has potential as an anticancer

and has an effect quite actively on inhibition of MCF-7 cancer cell proliferation

as well as having a strong effectiveness on pure extract.

Keywords: Andaliman, anticancer, breast cancer, MCF-7, MTT Assay,

secondary metabolites



OP-A-4

Biological Screening of Leaf and Fruit Extract (Ficus carica L)

using Brine Shrimp Lethality Test (BSLT) toxicity bioassay on

Arthemia salina Leach Larvae

Risa Purnamasari*1, Eva Agustina1, Nova Lusiana2, Miftakhur Rohmah1

1 Sains and Tekhnologi Faculty, UIN Sunan Ampel Surabaya, Indonesia. 2Psikologi and Health Faculty, UIN Sunan Ampel Surabaya, Indonesia.


Abstract

Brine Shrimp Lethality Test is the most convenient system for monitoring

biological activities of various plant species. This method is very useful for

preliminary assessment of toxicity of the plant extracts, it`s also used as a

preliminary test against anti cancer activity by looking at the death of larvae

caused by active compounds contained in the extract. This study aims to

determine the effects of the cytotoxicity of leaf and fruit methanol extract Ficus

carica L. using Brine Shrimp Lethality Test (BSLT) method. Ficus carica L.

Leaves and fruit are extracted by maceration method using methanol solvent.

However, some conditions have to be resolved, especially in standard

experimental conditions such as temperature, medium pH, salinity, and

aeration. Furthermore, toxicity test using shrimp larvae Artemia salina Leach.

Ficus carica L. extracts using this test have been determined in the

concentration range of 10, 100, 200, 500 and 1000 ppm of the examined Ficus

carica L. extract. Toxic effects of leaf and fruit extract can be seen between the

percentage of larval mortality and log concentration to obtain LC50. leaf and

fruits extract are toxic with values of each LC50 are 44.66 and 45.76. This is

because of compounds such as flavonoids, Triterpenoids and Sterols, Alkaloids

and Saponins contained in leaf extracts and fruits of Ficus carica L.

Keywords: cytotoxicity, Brine Shrimp Lethality Test, Ficus carica L.



OP-A-5

Designing of a less-immunogenic Nattokinase from Bacillus subtilis

subsp. natto: a computational mutagenesis

Yoanes Maria Vianney*, Stanley Evander Emeltan Tjoa, Sulistyo Emantoko

Dwi Putra




Abstract

Nattokinase is an enzyme produced by Bacillus subtilis subsp. natto that

contain strong fibrinolytic activity. It has potential to treat cardiovascular

diseases. In-silico analysis revealed that nattokinase is considered an antigen,

thus hindering its’ application for injectable therapeutic protein, in addition to

this protein is produced by bacteria and foreign to human. Various web servers

were used to predict B-cell epitopes of nattokinase both continuously and

discontinuously to determine which amino acid residues is responsible for the

immunogenicity. With the exclusion of the predicted conserve amino acids,

four amino acids were allowed to be mutated, which were S18, Q19, T242, and

Q245. Substitution mutation was done to lower the immunogenicity of native

nattokinase. Considering the stability of the mutated protein with the help of

Gibbs’ free energy difference, the proposed mutein was S18D, Q19I, T242Y

and Q245W. 3D validation, stability analysis of the protein, potential energy

measurement of molecular docking, and RMSF determination from molecular

dynamics analysis, the mutein displayed lower antigenicity and does not differ

from the native protein’s stability and structure.

Keywords: B-cell epitopes, Bacillus subtilis subsp. natto, Bioinformatics,

Immungenicity, In-silico mutagenesis, Nattokinase



OP-A-6

ALPHA MANGOSTIN EFFECT ON MDA LEVEL AND THE

PANCREATIC MORPHOLOGY Rattus novergicus INDUCED

ALLOXAN

Devyana Dyah Wulandari1, Zumrotul Mufidah1, Taslim Ersam2, Devyani Diah

Wulansari3

1University of Nahdlatul Ulama Surabaya, 2Institut Teknologi Sepuluh

Nopember, 3University of Surabaya


Abstract

Alpha mangostin compound is a single compound isolated from rind extract

Garcinia mangostana Linn, known to have an antidiabetic bioactivity. The

aimed of this research was to determine the effect of α-mangostin compound on

MDA and and rats pancreatic tissue induced with type 2 diabetes mellitus.

Methode: 24 Wistar male rats were divided into 4 groups, each group

consisting of 6 rats. The first group was set as positive control, rats injected

with single dose of 150 mg / kg alloxan. The second group was the negative

control, mean that no treatment. The third group injected with a single dose of

150 mg/kg alloxan and a-mangostin compund 10 mg/kg, while the last group

injected with a single dose of 150 mg/kg alloxan and 10 mg/kg standard drug

glibenclamide. All groups were monitored for 3 weeks and blood sugar levels

were measured using Glucosemeter. Blood samples were taken to measurement

of MDA and pancreas organ was removed for histological study. MDA level in

positive control group had a MDA value higher than the other groups (8.1771 ±

1.5614). MDA level in α-mangostin compound treatment group (6.9724 ±

1.1803), the negative control group (6.4906 ± 0.6464) and glibenclamide group

(6,6111 ± 0.7106). Islets of langerhans on pancreatic normal mice (control (-))

looked filling full with the endocrine cells spread throughout the pancreas.

While at the control group (+) there was a room-empty space (fewer the

number of beta cells) in islets of Langerhans. On the treatment groups those

were giving a-mangostin and drug compounds standard (glibenclamide)

showed improvement Langerhans β cells compared with a treatment group of

diabetic mice (control+). The administration of α-mangostin compound 10

mg/kg BW able to decrease MDA level 1,2031 nmol/dl. While based on

histological observations pancreas, administration of alpha mangostin and Drug

Compounds standard (glibenclamide) showed improvement compared

Langerhans β cells with a treatment group of diabetic rats.



Keywords: Alpha mangostin, Malondialdehyde (MDA), Diabetes Mellitus type

2, pancreatic histology



OP-A-7

In Vivo Efficiency of Collagen Extract from Sangkuriang Catfish

(Clarias gariepinus var. Sangkuriang) Skin on MDA Levels and

Macrophages Total in Burn Wound Healing

Andini Ary1, Handajani Retno2, Soetjipto2

1Faculty of Health, University of Nahdlatul Ulama Surabaya, Indonesia 2Departement of Biochemistry, Faculty of Medicine, University of Airlangga,

Surabaya, Indonesia


Abstract

Topical collagen on skin wound gift moist state that could stimulate re-

epithelization, proliferation and epithel cell migration to conduct growth factor

activity on wound area. One of source collagen is Sangkuriang catfish skin that

had low immunoreactivity in human body. The aim of this study was to

determine the influence of topical treatment with Sangkuriang catfish skin

extract on MDA serum and macrophage total on burn injury. Collagen of

Sangkuriang catfish skin were extracted by HCl 2% for 48 hours and

neutralized by NaOH 1 M. Sangkuriang catfish skin extract contained amino

acid of collagen that could repaire and stimulate skin cell. This study was an

experimental study that used twenty-four male Rattus novergicus strain Wistar

were divided into K3, P3, K10, and P10 groups. K3 group was treated with

aquadest topical as burn healing treatment for 3 days and K10 group was

treated with aquadest topical for 10 days. P3 group was treated with topical

collagen extract as burn healing treatment for 3 days, and P10 group was

treated with collagen extract for 10 days. The results showed that treatment by

collagen from Sangkuriang catfish skin could insignificantly decrease MDA

serum on 3rdday (K3 and P3 groups) and on 10th day (K10 and P10 groups).

Furthermore, it could decrease macrophage total insignificantly on 3rd day for

K3 and P3 groups but significantly decrease on 10 days for K10 and P10.

Keywords: Sangkuriang catfish, collagen, malondialdehyde, macrophage,

wound healing, burn injury



OP-A-8

The Effect Of Folic Acid Supplementation In Parental Stress Mouse

Toward NR3C1 Expression Alteration In The Offspring

Patricia*, Sulistyo Emantoko Dwi Putra, Ida Bagus Made Artadana




Abstract

Epigenetics is a pattern of inheritance of the traits of the parent to offspring

without DNA sequence alteration. Paternal programming is a pattern of

epigenetic inheritance issued by the father. Previous research on the "Overkalix

study" shows that grandfathers with abundant food availability increase

mortality and risk of diabetes and cardiovascular disease in grandchildren. Folic

acid is an antidepressant drug that works by lowering homocysteine and

increasing the methylation of DNA, proteins, lipids, and neurotransmitters. On

the other hand, increase in methylation in the NR3C1 gene due to reduction of

glucocorticoid receptor that leading to depression and other diseases. the effect

of folic acid supplementation in paternal stress toward NR3C1 expression in the

offspring isn’t known yet. In this study, the expression level of NR3C1 gene on

mice (Mus musculus Balb/c) from paternal stress supplemented by folic acid

was compared with the offspring of mice from paternal stress and control. The

results of gene expression analysis showed significant decrease in gene

expression in the offspring of mice from paternal stress compared to control (p

<0.01). In addition, there was a trend of decreased gene expression in the

offspring of mice from paternal stress supplemented by folic acid compared to

paternal stress (p <0.1).

Keywords: paternal programming, epigenetic, stress, folic acid, NR3C1



OP-A-9

In Silico Mutagenesis: Decreasing the Immunogenicity of

Botulinum Toxin Type A

Stanley Evander Emeltan Tjoa*, Yoanes Maria Vianney, Sulistyo Emantoko

Dwi Putra



Corresponding author: [email protected]

Abstract

Botulinum toxin serotype A is one prominent therapeutic enzyme, both for

clinical and cosmetic use. Since this protein is produced by bacteria, this

protein exhibit allergenic effect when subjected to human therapy. Protein

mutagenesis is one method to improve the characteristic of protein. However,

in-silico study is needed to give suggestion which amino acid should be

mutated, hence can save many cost and time. This paper gives suggestion

which residue of the Botulinum toxin serotype A is B-cell epitopes both

linearly and conformationally. By doing overlapping of those B-cell epitopes

with the excluded conserve sequence, 7 residues were allowed to be mutated.

There are two proposed mutein that showed a reduce in the antigenicity

probability, which are ΔE147, E510F, T1062F, ΔE1080, N1089M, ΔQ1090;

and ΔE147, E510F, T1062F, E1080W, N1089M, ΔQ1090. By doing the

validation analysis with various tools and flexibility with molecular dynamics

simulation of the 3D proposed muteins, both mutein was recommended. Both

protein has a lower antigenicity but similar in structure and stability compared

to the native protein.

Keywords: bioinformatics, botulinum toxin type A, B-cell epitopes prediction,

immunogenicity, in-silico mutagenesis



OP-A-10

Comparison of Food Intake Containing Vitamin D with BMI of

Geriatric Patients in Sidoarjo, East Java

Rivan Virlando Suryadinata*, Sajuni Wijaya, Risma Ikawaty

Medical Faculty of Surabaya University


Abstract

Previous studies showed relationship of vitamin D deficiency with obesity. This

study was based on observation of food intake containing vitamin D with BMI

geriatric patients in Sidoarjo, East Java. We used case control design with semi-

quantitative questionnaire. Eightythree obese geriatric patients and 83 non-

obese geriatric patients (4.82% thin, 84.34% normal, and 10.84% at risk) were

observed. Sampling technique was random sampling (non-probability

sampling) with purposive sampling method and tested by using Chi square.

From the obese group we found 76 patients had vitamin D deficiency and 7

patients had enough vitamin D supplementation, while in the non-obese group,

80 patients had deficiency of vitamin D intake, and 3 patients had enough

vitamin D (p = 0,192 (p> 0,05)). Thus we concluded, there was no significant

difference of food intake containing vitamin D on both group, obese and non-

obese geriatric patients.

Keywords: Vitamin D, geriatric, obesity



OP-A-11

Influenceof Folic Acid Diet on Maternal Stress to Offspring Stress

Level and NR3C1Expression

Natanya*, Sulistyo Emantoko Dwi Putra, Ida Bagus Made Artadana




Abstract

Stress has become the main topic on the subject of neurology and affective

disorder. In terms of epigenetics, parental stress may cause neuropsychiatric

difficulties to offspring. During stress, excess cortisol level happens duet a low

expression of glucocorticoid gene (NR3C1). This is inherited to offsprings by

supressing NR3C1 gene. To avoid stress exposure, folic acid intervention

became one of the option as antidepressant. In this research, stressed mice

model and the effect of folate diet is towards stress is made. Stress behaviour is

test using forced swim test (FST) method. Female parent with space stress and

darkness experienced stress, which can be seen from high immobile duration

compare to mice without stress (p<0.05). While folate diet does not show any

influence on the inheritance of levels of stress, seen by the difference in

immobile duration of stressed female parent and stressed with intervention of

folic acid diet (p=0,367). The result of this stressed model is then bred with

normal male parent and its behaviour and level of NR3C1 expression is

observed. On offspring, it is found that there is no significant different between

the offspring of maternal stress and normal (p=0,174). Furthermore, there is a

significant decrease (p<0,05) on the expression of NR3C1 on offspring of

maternal stressed pair. This result shows that the potential to experience stress

of offspring with a stressed female parent is higher than offspring of normal

female parent.

Keywords: Glucocortioid receptor, epigenetic, folic acid, stress level, FST



OP-A-12

Facile Green Synthesis of Silver Nanoparticles from Ethanol

Extract of Plantago major L. and its antibacterial activity

Sukweenadhi Johan1, Setiawan Kezia Irianti2ϕ, Kartini2, Tjandrawinata

Raymond Rubianto3, Avanti Christina2*

1Department of Plant Biotechnology, Faculty of Biotechnology, University of

Surabaya, Jalan Raya Kalirungkut, Surabaya 60293, East Java, Indonesia 2Department of Pharmaceutics, Faculty of Pharmacy, University of Surabaya,

Jalan Raya Kalirungkut, Surabaya 60293, East Java, Indonesia. 3Dexa Laboratory of Biomolecular Science, Titan Center, 3rd Floor, Boulevard

Bintaro, Block B7/B1, No. 5, Bintaro Jaya Sector 7, Tangerang 15224,

Indonesia


Abstract

Nanomaterials from biological sources have received large attention due to

broad range of utilization and less harmful for humans, animals and

environmental-friendly. Our aim is to provide the nanoparticles to control

infections in diabetic foot ulcer. In this present study, silver nanoparticles

(PmAgNPs) were synthesized through green synthesis approach, using

Plantago major L leaves extract. UV–visible absorption spectroscopy,

Scanning Electron Microscopy, X-Ray Diffractometer and Fourier Transform

Infra-Red spectroscopy analyses confirmed synthesis of silver nanoparticles.

The stable non-aggregating nanoparticles were spherical in shape with an

average size of 287.9 nm and has maximum absorbance at a wavelength of

436.6 nm. Characterization of the antimicrobial activity of synthesized

nanoparticles by disk diffusion method showed strong growth inhibitory

activity against all tested bacteria commonly infected to ulcer patient, ie.

Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. As

evidence, the inhibition zones of PmAgNPs ranged from 57 to 78 mm. Less

time, cost efficient and eco-friendly synthesis of biologically active silver

nanoparticles from Plantago major L leaves extract not only cut down

laborious downstream process, but also provides a reliable source of safe

nanomaterials with potential application in diabetic foot ulcer treatment.

Keywords: Diabetic Foot Ulcer, Green synthesis, Silver Nanoparticles,

Plantago major L., antibacterial activity




OP-A-13

In Vitro Evaluation of Chitosan-DNA Plasmid Complex Encoding

Jembrana Disease Virus Tat Protein as a Vaccine Candidate

Asmarani Kusumawati1,2*, Januar Ishak2, Lalu Unsun-Nidhal2 and Ronny

Martien3

1Department of Reproduction, Faculty of Veterinary Medicine, Universitas

Gadjah Mada, Jalan Sekip unit II, Yogyakarta, 55281, Indonesia, email:

asmaranikusumawati [email protected]; 2Research Center for Biotechnology, Universitas Gadjah Mada, Jalan Teknika

Utara, Yogyakarta, 55281, Indonesia; 3Departement of Pharmaceutics, Universitas Gadjah Mada, Jalan Sekip Utara,

Yogyakarta, 55281, Indonesia


Abstract

The development of Jembrana disease vaccine is importance to prevent the loss

of Bali cattle industry in Indonesia. Delivery system is a vital factor for the

success of DNA vaccine in inducing immunity. This study aims to prepare a

Jembrana DNA vaccine encoding the tat gene in pcDNA3.1 and to test the

success of its delivery in cells using DNA-chitosan complex. Cloning of the

pcDNA3.1-tat has been successfully performed on E. coli DH5α and confirmed

by PCR, restriction analysis and sequencing. The propagated plasmids were

prepared as DNA-chitosan complex and physiochemically characterized using

Particle Size Analyzer. Complex with a 1:2 (wt/wt) ratio of DNA and chitosan

have a mean diameter of 268.5 nm and zeta potential +25.1 mV. In this

research, the addition of TPP as cross linker agent (chitosan:TPP = 1:2 wt/wt)

results in the increase of mean diameter to 718.1 nm and in the decrease of zeta

potential to 4.7 mV. These two complexes were transfected to the HeLa cell

line as in vitro model of eukaryotic cells. The DNA vaccine was successfully

delivered into cells, shown by the presence of specific RT-PCR product (149

bp) from total RNA of transfected HeLa. The real-time PCR analysis showed

that the delivery with chitosan complex resulted in higher tat mRNA expression

when compared with delivery using chitosan-TPP complex.

Keywords: DNA-chitosan complex, Jembrana disease, tat gene, TPP



OP-A-14

THE RELATIONSHIP BETWEEN ASTHMA CONTROL -

METHYLXANTHINE USED AND CYP1A2*1F GENE

POLYMORPHISMS

Mariana Wahjudi1*, Doddy de Queljoe2, Amelia Lorensia2, Muhammad

Erdiansyah1, Yovita Selma Desiyana2, Eki Nuri Fauziyah2

1Laboratorium Purifikasi dan Biologi Molekuler, Fakultas Teknobiologi,

Universitas Surabaya, Jl Raya Kalirungkut, Surabaya 2Laboratorium Farmasi Klinis – Komunitas, Fakultas Farmasi, Universitas

Surabaya, , Jl Raya Kalirungkut, Surabaya


Abctract

The most frequently used methylxantine groups are theophylline and

aminophylline. Both medicines are rarely used in others countries because of its

narrow therapeutic effect with ADR (adverse drug reactions) risk. However in

Indonesia, both medicines rarely caused any serious problems and even rarely

cause ADR. This individual reaction is likely influenced by the CYP1A2*1F

gene polymorphisms that contributes to theophylline metabolism in the liver.

Therefore, this study was aimed to investigate the correlation between asthma

control and methylxanthine used with CYP1A2*1F gene polymorphism. This

research is a cross-sectional study with purposive sampling in the asthma

outpatient setting. Data of asthma control and frequency of methylxanthine

medication was taken by interviewing, while the CYP1A2*1F gene

polymorphism was detected by polymerase chain reaction-restriction fragment

length polymorphism (PCR-RFLP) technique. The data were analyzed using

Chi-Square test. The result from the 32 adult-asthmatic-outpatients revealed

that there was no relationship between asthma control with the CYP1A2*1F

gene polymorphisms (sig. = 0.433). However, there was a positive correlation

between ADR occurrence in theophylline/aminophylline medication with the

CYP1A2*1F gene polymorphisms (sig. = 0.038). It is therefore necessary for

pharmacists to monitor the use of theophylline / aminophylline regarding to the

genetic and environmental influences.

Keywords: Asthma control, methylxantine, CYP1A2*1F gene polymorphisms




AGRICULTURE



OP-B-1

Plant Regeneration through Somatic Embryogenesis from Callus of

Vanda tricolor Lindl. var. pallida

Popy Hartatie Hardjo*, Wina Dian Savitri and Ivon Grace Paulin

Faculty of Biotechnology, University of Surabaya

Jalan Raya Kalirungkut, Surabaya 60292, East Java, Indonesia


Abstract

Somatic embryogenesis is a process where cells undergo sequence of

development which similar to zygotic embryo’s development. In this study, we

developed a protocol to induce indirect somatic embryogenesis from the basal

leaf segments of Vanda tricolor Lindl. var. pallida. Embriogenic calli obtained

from previous experiment were regenerated to form somatic embryo on half-

strength MS medium supplemented with (0.05-0.2 mg.L-1) BAP or in

combination with 0.01 mg.L-1 NAA. Embriogenic calli cultured on 0.05 mg.L-1

BAP incorporated with 0.01 mg.L-1 NAA were able to establish 90% somatic

embryos (SEs)’ structure at 30 days of culture period. The pattern of SEs’

development started from embryogenic callus to form pro-embryo, followed by

globular phase at 10 days of culture. Globular embryo elongated to form

suspensor at 20 days of incubation period, and completed embryo. The fastest

SE’s germination occurred on the half-strength MS medium with the addition

of 0.02 mg.L-1 BAP.

Keywords: somatic embryogenesis, Vanda tricolor Lindl. var. pallida




OP-B-2

Influence of Types and Concentration of Natural Pesticides to

Mortality of Red Mite (Panony chuscitri McGregor)

Dyah Roeswitawati*1, Iva Kristova2 Muhidin1, Otto Endarto 3

1 Lecturer at Agotechnology Departement of Muhammadiyah University of

Malang 2 student at Agotechnology Departement of Muhammadiyah University of

Malang 3 Field Supervisor Indonesian Citrus and Tropical Fruits Research Institute

Batu, East Java


Abstract

Red mites (Panonychuscitri McGregor) is one of the plant pest attacking leaves

of citrus. That pest attack leaves on citrus plants leads to symptoms: patches of

sterile or brown on fruit and yellow or brown spots on the leaves of oranges.

Farmers often control this pest with drugs or chemical pesticides such as

insecticides. Increased pest resistance against pesticides (resistance), making it

less precise and can lead to poisoning for humans and ecosystems, the

environment becomes unstable / unbalanced. Persistent pesticides leave

residues that are difficult to clean. Usually farmers control the pest using

chemical pesitisida and cause environmental damage due to toxins contained in

the pesticide material. Chemical pesticides also result in increased pest

resistance against pesticides. Persistent pesticides also leave residues that are

difficult to clean in plants so that natural pesticides are needed as an alternative

to chemical pesticides. The purpose of this research is to know the effect of

vegetable pesticide concentration of soursop (Annona muricata) leaf, papaya

(Carica papaya) leaf, Chrysanthemum leaves to red pest mortality and

persistence of vegetable pesticide. The results showed that pesticide

concentration 5% resulted in 100% mortality of imago phase and instar phase 1

at 48 hours observation. The highest mortality rate is the treatment of papaya

leaves pesticide 5% concentration in the imago phase that is a number of 29.67

Keywords: Annonamuricata , Carica papaya, Chrysanthemum, mortality,

persistence, red mites



OP-B-3

Effect of salinity on biomass allocation and whole plant mass of rice

(Oryza sativa L) related to growth at panicle initiation and heading

stages

Niki Habibi*1, Nafisah2, Indah Trisnawati D.T.1, Mukhammad Muryono1

1Departement of Biology, Institut Teknologi Sepuluh Nopember, Kampus ITS-

Keputih Sukolilo, Surabaya 60111, Indonesia. Telp. +62 31-5963857, Fax +62

31-5963857, E-mail [email protected]. 2Indonesian Agency for Agricultural Research and Development Ministry of

Agricultural, Ragunan street 29 Pasar Minggu, Jakarta Selatan 12540,

Indonesia.Phone +62 21-7806202


Abstract

Rice (Oryza sativa L.) is the most important staple food and provided most of

the daily calories for the world population. In South East Asia, 40% of rice

production is from a deltaic region that is vulnerable to salt stress. Meanwhile,

the suboptimal saline area in Indonesia has wide open agricultural

extensification opportunities, covering an area of 0.4 million hectares. Rice is

considered to be moderately sensitive to salinity. The sensitivity of rice to

salinity stress varies with the growth stage. A physiological response was

developed for characterizing varieties adapted to salt-affected areas. Four

varieties we evaluated in the field under the saline condition, with two soil

salinity level, low saline on 4 dSm-1 and high saline on 8 dSm-1. Plant mass,

green leaf number, shoot mass, and roots mass were measured at the vegetative

stage. For four varieties tested at both saline conditions, the [saline] x [variety]

interaction was significant for plant mass, shoot mass, root mass, but there was

no significant for green leaf number. Interaction with a stage was also

significant for all parameters observed. Plant mass at both stages was closely

correlated with root mass and leaf number over salinity level and varieties.

Root mass and leaf number might be a target in future rice breeding under salt

stress for increasing dry matter production.

Keywords: Rice (Oryza sativa L.), Salinity, Biomass allocation, Whole plant

mass, Growth.



OP-B-4

Study of Ccs and pAMT Gene of Genotype 1 Cayenne Pepper

(Capsicum frutescens L.) Original Type and Mutant

Nur Rahmattullah*, Wahyuningyan Arini, Ginting Dwi Setyawan, Estri Laras

Arumingtyas

Department of Biology, Faculty of Mathematics and Sciences, University of

Brawijaya, 1st Veteran street, Malang, Indonesia


Abstract

Cayenner pepper (Capsicum frutescens L.) is a potential commodity cultivated

in Indonesia and effort to establish superior varieties have been done, one of

them by using ethyl methanesulphonate (EMS) as mutagent. This study aimed

to to (1) analyze the change of the sequences of Ccs and pAMT genes in the G1

cayenne pepper original type (OT) and mutant; (2) investigate carotenoid and

capsaicin content that might correspond to gene mutation. The Ccs and pAMT

from G1 OT and mutant were analyzed through sequencing and aligned with

Capsicum annuum from GeneBank. Carotenoid and capsaicin content were

measured from three different fruit age, i.e. 30, 45 and 60 days after pollination

(DAP). The change caused sequence of pAMT and Ccs gene of G1 cayenne

pepper different from the G1 OT, but same as Capsicum annuum cv. Himo.

The alternation of C (cytosine) to T (thymine) nucleotide in 809 bp of Ccs that

cause isoleucine-arginine alternation might has the impact physiologically.

Overall, compared with OT plant, carotenoid and capsaicin in mutant showed

higher value and had shorter optimum fruit age based on cut off point analysis.

Hence, mutation through EMS might establish superior varieties in future.

Keywords: cayenne pepper, carotenoid and capsaicin, days after pollination,

gene profile, nucleotide alternation, reverse mutation



OP-B-5

Menthol from stem and leaf invitro Mentha piperita

Bintang Kerta Wijaya*, Popy Hartatie Hardjo, Sulistyo Emantoko Dwi Putra




Abstract

The need for menthol in Indonesia is increasing annually, but it is not followed

by increasing in the availability in the country so the number of imports is

increasing each year. Therefore, in this research we conducted a study using

plant tissue culture techniques to produce secondary metabolite especially

menthol using shoot multiplication. Single node of Mentha piperita were

inoculated on three kinds of growth medium, which are MS + 0,1 ppm NAA +

0,1 ppm BA, MS + 0,5 ppm BA, and MS + 2 ppm BA. Based on fresh weight

and morphological observation, MS + 0,1 ppm NAA + 0,1 ppm BA was the

best growth medium. Based on the previous experiment, the study was aiming

to continue observing the concentration of menthol found in Mentha piperita

shoot. Stems and leaves of Mentha piperita were dried in room temperature, the

dried stems and leaves were extracted using steam distillation method with ratio

0,3 grams of dried stems and leaves in 25 mL aquadest. The crude extract was

analysed by gas chromatography (HP 6890) using INNOWAX 19095N-123

column. The results showed that two weeks was the best amount of time to

obtain highest concentration of menthol specifically 1,218.5 ± 47.1 ppm (yield

= 9.748%).

Keywords: Mentha piperita, menthol, shoot multiplication, steam distillation,

gas chromatography



OP-B-6

Analysis of Abscisic Acid, Salicylic Acid and GABA in Several Oil

Palm Progenies in Drought Stress

Susy SAADAH*, Endri PURWANTI, Randi Abdur ROHMAN, Nurita

TORUAN-MATHIUS1, Tony LIWANG1

PT SMART Tbk


Abstract

Metabolites are key players in signaling environmental stress conditions. This

study aims to compare the concentration level of abscisic acid, salicylic acid,

and gamma-aminobutyric acid (GABA) among tolerant and susceptible oil

palm progenies of drought stress. The experiment was arranged in factorial

randomized complete block design consisted of three factors: drought stress

(watering and non-watering), progenies (S1, G2, G3, G4, G5 and G6), and

duration of treatments (0, 7 and 14 days). The samples were taken from leaves

and roots tissues. The result showed that abscisic acid, salicylic acid, and

GABA were significantly elevated in leave samples of tolerant progenies at 7-

days of non-watering treatment. However, abscisic acid, salicylic acid, and

GABA were not significantly produced in roots samples of tolerant progenies at

14-days of non-watering treatment.

Keywords: abscisic acid, drought, GABA, oil palm, salicylic acid



OP-B-7

Uptake of Macro Nutrients and Growth of Mahang plants

(Macaranga gigantea)

Dwi Susanto*1, Rudianto Amirta2

1Department of Biology, Faculty of Mathematics and Natural Sciences,

Mulawarman University, Jl. Barong Tongkok No. 4, Gunung Kelua,

Samarinda, Indonesia 2Faculty of Forestry, Mulawarman University, Indonesia, Jl. Ki Hajar

Dewantara Kampus Gunung Kelua, Samarinda, Indonesia


Abstract

Macaranga gigantea plant is one species of the secondary tropical rain forest in

East Kalimantan. This plant in addition to potentially as a medicinal plant, also

has potential as industrial wood raw materials. The study was conducted for 6

months at the Botanical Garden of Mulawarman University Samarinda. The

aims of this study is to determine the uptake of macro nutrients and the growth

of M. gigantea plant with application of NPK fertilizer. The research was done

by randomized block design, the fertilizer treatment consisted of four levels, P0

= 40 g, P1 = 80 g, P2 = 120 g and P4 = 160 g per plant, each with 20

replications. The results showed that fertilization increased nutrient uptake and

M. gigantea plant growth. At the beginning of growth, this plant accumulates a

lot of magnesium, potassium, calcium, followed by nitrogen and at least is a

phosphorus nutrient. Fertilization application also increases plant height, leaf

number, stem diameter and crown diameter.

Keywords: Macaranga gigantea, growth, nutrient uptake



OP-B-8

The Evaluation of Breeding Value and Most Probable Producing

Ability for the Basis Selection of Ettawa Croosbred Goat at Malang

Region, East Java, Indonesia

Aris Winaya*, Suyatno1 and Nurul Fauzi

Faculty of Agriculture and Animal Science, University of Muhammadiyah

Malang


Abstract

Ettawa crossbred goat is Indonesia local breed goat that genetically has also

advantages regarding milk production. But, these goats have not maximally

developed for milk production. The continual selection of the breed was

important to improve the productivity of Ettawa crossbred. The selection

program can be an effective effort to increase the milk production of Ettawa

crossbred goat. The Breeding Value (BV) and Most Probable Producing Ability

(MPPA) could be considered on selection program on goat breed. The purpose

of this study was to determine the differences in milk production of Ettawa

crossbred in Malang region and to know the BV and MPPA values of Ettawa

crossbred goats. This research was conducted by direct survey method on goat

farmers in the sample areas. The second lactation of the milk production was

stated as the data variable. The analysis of variance from nested design showed

that any significance different (P<0.05) in milk production between

regency/city population and very significant (P<0.01) in milk production

between districts population of Malang region. The Least Significant

Difference (LSD) test on sub-district of Malang Regency showed that both

Ampelgading with Singosari sub-district and Lawang with Singosari sub-

district were high significance different (P<0.01), but Ampelgading with

Lawang sub-district was not significantly different (P>0.05). At Batu Regency

showed that Bumiaji with Pesanggrahan sub-district was significantly different

(P<0.05) while Bumiaji with Junrejo and Junrejo with Pesanggrahan were not

significantly different (P>0.05). The highest BV was 0,389 litre from Bumiaji

sub-district and the highest MPPA was 0.595 liter from Bumiaji sub-district

also. So, from the BV and MPPA values Ettawa crossbred goat from Bumiaji

sub-district of Batu Regency could be chosen as the basis population on the

breeding program of dairy milk in Malang region.

Keywords: Breeding Values, dairy milk, Ettawa goat, Malang region, MPPA



OP-B-9

IN VITRO MULTIPLICATION OF FIG (Ficus carica L.) FOR

COMMERCIALLY INDUSTRY

Ivon Grace Paulin*, Johan Sukweenadhi, and Popy Hartatie Hardjo

Faculty of Biotechnology, University of Surabaya

Jalan Raya Kalirungkut, Surabaya 60292, East Java, Indonesia

* Corresponding author: [email protected]

Abstract

The most common fruit has planted in Turkey and Mediterranean region is

fig fruit (Ficus carica L.). It has been an important food crop for thousands

of years and in the world market, there is an increasing demand for fresh figs

and a stable demand for dried figs. This fruit also contain a lot of benefit

nutritional and phytochemistry function. So, probably we have to develop

this edible fruits in Indonesia, which commonly have the same tropical like

in Mediterranean region. Furthermore, the technique such as in vitro culture

has advantage of large-scale production and providing plantlets as seeds. In

this review, we discuss several results of propagation fig fruit from various

condition of explants and combination growth hormone in media in tissue

culture Ficus carica L. Multiplication from shoots used combination MS + 1

mg /L IBA + 1 mg /LGA3 + 5 mg /L BA. Callus induction development in

culture medium supplemented with 2 mg/l TDZ and 4 mg/l IBA. In the other

hand, activated charcoal, ascorbic acid 0.1% and phloroglucinol were added

to prevent negative effect of polyphenols from explants. Since several

research in tissue culture of Ficus carica L. and they have been successful

for clonal multiplication by shoot or organogenesis by callus induction. It

still needs the development of Fig protocol for each cultivar to support other

research and can be used more easily by the community in developing

commercial needs.

Keyword: Ficus carica L., callus induction, in vitro multiplication



OP-B-10

Mild Alkaline Pretreatment on Sugarcane Bagasse: Effects of Time

and Lime Ratio to Dry Bagasse

Lieke Riadi1, Maria G.M. Purwanto2, Jesslyn Pratiwi1, Yoelsando Hansen1

1Chemical Engineering Department, University of Surabaya, Surabaya, 60293,

Indonesia

2Faculty of Biotechnology, University of Surabaya, Surabaya, 60293,

Indonesia

* Corresponding author: [email protected]

Abstract

Sugarcane waste is organic waste produced at about 8,000,000 tons/year.

This lignocellulosic waste can be used for raw material of bioethanol

manufacture through pretreatment, hydrolysis, and fermentation process.

This study aims to determine the yield (g/g) of glucose produced from

pretreatment by using lime (Ca(OH)2) and enzymatic hydrolysis. The

variables used in this research were pretreatment time and lime ratio to

bagasse in the pretreatment step. The results showed that pretreatment time

gave a stronger effect on the glucose yield compared to lime:bagasse ratio

did. Longer pretreatment time up to 180 min increased the glucose content

of the pretreated bagasse, but after 180 min of pretreatment, glucose content

was decreased. While higher ratio of lime to bagasse in pretreatment process

decreased the glucose content. The best glucose content (0.071 g/g) was

obtained from pretreatment process with a ratio of 0.5 g Ca(OH)2/g bagasse

for 180 min and ethanol content of 5.704 ± 0.15 g/L.

Keyword: Bagasse, Alkaline pretreatment, Bioethanol, Enzymatic

hydrolysis



ENVIRONMENT &

ENERGY



OP-C-1

Utilization Cytochrome B Gene for Phylogeny Analysis of Cuora

amboinensis to Prevent Extinction: Preliminary Study

Dwi Martha Nur Aditya*1 and Mohamad Amin2

1Medical Faculty of Ubaya University

2State University of Malang


Abstract

Cuora amboinensis is widespread distributed and yet these species is classified

as vulnerable since they are at high risk of extinct. It is also known that C.

amboinensis does not migrate seasonally or to any geographically significant

extent which causes chances of inbreeding even greater. Phylogeny analysis are

required to reveal the kinship relationship of Genus Cuora, so it can be used as

a preliminary study of conservation by determining outbreeding. We compared

Cytochrome b in C. amboinensis samples from Kendari, Toli-toli, and Ambon

regions to that C. picturata (Vietnam), C. galbinifrons (China), C.

flavomarginata (China), C. serrata (China), and C. mouhotti (China).

Technology we used including DNA preparation, Primer Design, Polymerase

Chain Reaction (PCR), horizontal electrophoresis, and sequencing. We than

compared the Cyt b sequences to the reference species obtained from the NCBI

Genbank. Further, phylogeny analysis is performed by using MEGA 6.0 with

Maximum Likelihood (ML) methods. The result showed close kin relationship

between of C. amboinensis of Ambon, Kendari, and Toli-toli regions with most

of genetic distance below 2.1, while Ambon-I and Kendari-11 have measured

the longest 33.63 and 38.18, respectively.

Keywords: Cuora, genetic distance, phylogeny analysis, Cyt b



OP-C-2

Profile of Antibiotic CompoundsfromTrichoderma harzianumin

Controlling Aspergillus flavuson Bali Lontar

I B G Darmayasa*, Retno Kawuri

Microbiology Laboratory Faculty of Mathematics and Natural Sciences,

Udayana University


Abstract

Lontar is a descent of ancestors that should be preserved. The lontar script

contains the script of Balinese script that is classical or ancient which is often

referred to as manuscript and is a cultural tradition of writing in Bali that tend

to be sacred and religious (Geriani, 2010, Sedana et al., 2013). Lontar stored in

the community did not escape the damage and one cause of the damage is

Aspergillus flavus. Trichoderma is a group of fungi that are believed to have the

ability to inhibit the growth of other microorganisms. The objective of the study

was to find out the profile of antibiotic compounds produced by Trichoderma

sp. which will be utilized in controlling A. flavus on the palm of Bali. Isolation

of A.flavus and Trichoderma sp. done by using plating method with dilution

series.Dual cultural method is used to determine the capability of Trichoderma

sp. against A.flavus. Extraction filtrate of Trichoderma sp. using diffusion well

method. Profile of antibiotic compounds Trichoderma sp. known by using

colomn technique and GCMS (Gas Chromatography Mass Spectrometry). The

result of fungal isolation on lontar was identified as A. flavus and on peanut

plant rhizosper was identified as Trichoderma hazinarum which had the ability

to inhibit A. flavus by 69,5 ± 1.56%. The extract of the filtrate obtained is able

to form a zone of 15.32 ± 0.277 mm.The identified 15 compounds present in

the filtrate extract were suspected to be antibiotics ie Benzene, 1,3,5-trimethyl-

(CAS) 1,3,5-trimethyl; 2-Azabicylol (3,2,1) octan-3-one; 5,6-Dihydro-4-

methyl-2H-Pyran-2-C; 2-Butenoic acid, 2-methyl- (CAS) 2-methyl-2; 2H-

Pyran-2-one, tetrahydro-4-hydroxy-4-methyl; 2-Bromo dodecane; Lyxitol, 1-O-

nonyl; Pentadecane, 3-methyl- (CAS) 3-methylpentax; Trichloroacetic acid,

tridecyl ester; Heptadecane, 3-methyl- (CAS) 3-methylhepta; n-Hexadecanoic

acid; Nonadecane, 3-methyl- (CAS) 3-Methylnonad; Octadecanoic acid; 3-

Methylheneiccosane and Heptadecane, 3-methyl.

Keywords: Antibiotic, Trichoderma harzianum, Aspergillus flavus, extract,

filtrate



OP-C-3

Plasmid Curing Analysis of Chromate Reducing Bacteria Isolated

from Serpentine Soil

Badruzsaufari*, Aisyah, Hasrul Satria Nur, and Dindin Hidayatul Mursyidin

Department of Biology of Faculty of Sciences and Mathematics of the

Lambung Mangkurat University.

*Corresponding author :[email protected]

Abstract

Chromate resistant bacteria that isolated from Serpentine soil of Mandiangin and

Awang Bangkal villages of Kalimantan Selatan were capable to reduce

chromium hexavalent (Cr6+) into non-toxic chromium species (Cr3+). It is

suggested that the bacteria have a potential to be developed as biological agents

in bioremediation of chromium contamination. The chromium resistant

mechanisms of the bacteria which involve the responsibility of their plasmids

should be revealed. The research included identification of the bacteria isolates

using 16 S rRNA gene sequences, examination of the isolate chromium

reduction capability, and elimination of the bacterial plasmids by culturing the

isolates on 0.1 % SDS containing LB media. Two bacterial isolates AB13 and

AB56ACr10 were found to have 99% of their 16 S rRNA gene sequences

similar to Bacillus cereus while the isolate M2Cr10 showed 99% similarity to

Acinetobacter radioresistens. All isolates were able to reduce 13 mg.L- Cr (VI)

up to 90 % for 24 H incubation at 30oC. The isolate AB56ACr10 could resist the

present of 150 mg.L- of Cr (VI) in the growth media for 24 H, but the other

isolates could only tolerate 100 mg.L- of chromium hexavalent. The three

isolates had 2 plasmids which 55 kb and 10 kb in size of which the latter was

removed from the bacterial cell when growing on 0.1 % SDS containing LB

media. The plasmid cured isolates could not be able to grow on 50 mg.L- of Cr

(VI) contaning media and reduced their resistance to ampicilin, streptomycin,

and chlorampheniol antibiotics.

Keywords: bacteria, bioremediation, chromium, plasmid curing, serpentine

soil



OP-C-4

Effect of Cycle Time on Biokinetics Parameters in Tapioca

Wastewater Treatment Using Sequencing Batch Reactor

Happy Mulyani*, Gregorius Prima Indra Budianto

Setia Budi University, Jl Letjend Sutoyo, Mojosongo, Surakarta, Indonesia,


Abstract

In this work, the effect of cycle time on kinetics parameters of sequencing

batch reactor (SBR) system of tapioca wastewater treatment was evaluated.

First, the SBR was operated under three different variations of filling time (0.5,

1, 1,5 hour) with an aeration time of 9 h. After reached a steady state, liquid

samples of SBR system at one-half hour intervals were collected for analysis of

pH, Chemical Oxygen Demand (COD) and Mixed Liquor Suspended Solids

(MLSS) for optimum filling time determination. The optimum filling time

results in SBR operation with the best values of biokinetics parameters

considering pH and substrate inhibition. Then, SBR was operated once again

with the cycle period: filling (optimum condition at first operation), aeration (9

h), and settling (9 h) to determine the effect of time of aeration and

sedimentation on poly-β-hydroxybutyrate (PHB) yield. Research results shows

that SBR operation with filling time 1 h was achieved best values of biokinetics

parameters. The biggest PHB yield is achieved in aeration phase of SBR.

Excessive aeration time and settling time addition contributes to PHB yield

decrease.

Keywords: Biokinetics Parameters, Cycle Time, Poly-β-hydroxybutyrate,

Sequencing Batch Reactor, Tapioca Wastewater



OP-C-5

The utilization of tofu waste for the cultivation of Spirulina for

biomass production

Yalun Arifin*1, Faisal Amri Tanjung2, and Ryanto Kurniadi3

1Department of Food Business Technology, Prasetiya Mulya University,

BSD Raya Utama, Kabupaten Tangerang 15339, Indonesia. 2Center for Studies on Hasyim Asy'ari Thoughts, Universitas Hasyim Asy'ari,

Jalan Irian Jaya No. 55, Tebuireng, Jombang, East Java, 61471, Indonesia 3PT. Nugen Bioscience Indonesia, Jalan Parangtritis Raya blok A5E/12, Ancol

Barat, Jakarta 14430, Indonesia


Abstract

Spirulina (Arthrospira platensis) is one of the algae with market that is

developing rapidly. The application includes for food supplement, animal feed,

cosmetics and production of specialty chemicals obtained during the extraction

of the algae biomass. The current market, however, is limited by the cost of

production, particularly when synthetic chemicals are used as the growth

medium. The use of industrial waste and agricultural by-product may reduce

the production cost. Our research is using liquid waste obtained from tofu

manufacturing process for production of Spirulina biomass. In this media, the

cell performed mixotrophic metabolism that allowed higher cell productivity.

The growth in 16- and 25-fold dilution of the liquid waste produced highest

biomass productivity and cell concentration at 14 mg/l-day and 0.1 g/l, nearly

three times higher than the values obtained from the cultivation in a control

medium using synthetic chemicals. Higher concentration of liquid waste

inhibited the cell growth. The biomass composition and the cellular

phycocyanin level of the cell growing in the tofu waste is similar to the

composition produced from growing in the control medium. This utilization of

the liquid tofu waste reduced the cost for medium until 40 % lower. These

results demonstrate the potential of using liquid waste from tofu manufacturing

process as an alternative low cost and efficient growth medium for Spirulina

biomass production.

Keywords: Spirulina, tofu waste, low cost medium, algae biomass



OP-C-6

Thermostable Chitinase-Producing Bacterium from Ijen Hot Spring

– Indonesia: Isolation, Identification and Characterization

Ruth Chrisnasari*, Dian Paulina, Liony Priscilla Sutanto,

Tjandra Pantjajani, Maria Goretti Marianti Purwanto

Department of Biology, Faculty of Biotechnology, University of Surabaya,

Jalan Raya Kalirungkut, Surabaya 60292, Indonesia

*Corresponding author: ruth_c @staff.ubaya.ac.id

Abstract

The high pharmaceutical industrial demand of thermostable chitinase with new

desirable characteristics has lead to the exploration of chitinolytic bacteria from

extreme environment. Therefore, in this study chitinase-producing bacteria

were isolated from Ijen hot spring and screened. The highest chitinolytic

activity bacterium was identified by 16S rRNA gene sequencing and its

characteristics were confirmed by morphological and physiological analyses.

The growth and chitinase production of the identified isolate were further

characterized under variation of agitation and aeration. The molecular,

morphological and physiological analyses confirmed that the isolated bacterium

belonged to Bacillus licheniformis. This isolate produced huge amount of

chitinase on Thermus colloidal chitin medium at 50 °C within 30 hours. The

highest growth and chitinase production were recorded at 3 vvm aeration and

300 rpm agitation. Chitinase produced by this isolate was evidently proofed as

thermostable through its high residual activity after several thermal stability

tests.

Keywords: isolation, characterization, thermostable chitinase, Bacillus

licheniformis, aeration, agitation



FOOD & NUTRITION



OP-D-1

Effect of Phosphoric Acid Pretreatment on Characterization of

Gelatin from Broiler Chicken Bones

Dewi Yuliani*, Akyunul Jannah, Anik Maunati, Hanifah Hasna Fauziyah

1 Universitas Islam Negeri Maulana Malik Ibrahim Malang


Abstract

Chicken bone is one of the halal alternative sources of collagen fiber. This

collagen, one kind of protein, can be hydrolyzed to form gelatin. The objective of

this research was to extract and characterize chicken bone gelatin. Chicken bones

were pretreated with phosphoric acid in varying concentrations and curing times.

The concentration of phosphoric acid was 8, 9 and 10%, while curing time was

12 and 24 hours. Then, gelatin was extracted using hot water before the yield and

physicochemical properties were examined. The result showed phosphoric acid

9% with 24 hours gave the highest yield, 15.4±0.94%. Water content, ash

content, and pH of chicken bone gelatin were 3.96±1.46%, 24.0±6.36%, and

5.74±0.003%, respectively. Other physiochemical properties were 372.29±4.62%

of gel strength and 54.21±1.24% of protein content. Based on Indonesia National

Standard (SNI), water content and pH value fulfilled the requirement, but ash

content was out of the requirement. Value of gel strength is higher than British

Standard gave rigid and solid texture. Infrared spectra of chicken bone gelatin

had the most with vibration peak at the wavenumber of 1639.74 cm-1 to the

amide I, of 1545.94 cm-1 to the amide II, of 1129.42 cm-1 to the amide III, of

2.2927.98 cm-1 to the amide B, and 3456.26 cm-1 to the amide A.

Keywords: Characterization, chicken bone, gelatin extraction, FTIR,

phosphoricacid, physicochemical properties.



OP-D-2

Molecular Characterization of Potentially Probiotic Bacterium

Isolate from Mackerel Fish (Rastrelliger sp.)

Yulia Sari Ismail*1, Febriani2, CutYulvizar3, Rasistia Ramadhani3

1 Biology Department, Mathematics and Science Faculty, Syiah Kuala

University, Banda Aceh, Indonesia. 2 Chemistry Department, Mathematics and Science Faculty, Syiah Kuala

University, Banda Aceh, Indonesia. 3 Biology Department, Mathematics and Science Faculty, Syiah Kuala

University, Banda Aceh, Indonesia.


Abstract

The use of probiotics in aquaculture can improve the quality and quantity of the

fishes. One of potencial probiotic bacterium isolate from gastrointestine of

Mackerel fish (Rastrelliger sp.) that isolated in this research were LUI-04. This

study was aimed to determine the type of bacterium isolate using nucleotide

sequence homology compared to other microorganisms in GenBank. The 16S

rRNA genes of LUI-04 bacterium isolate were analyzed. The results of

electrophoresis showed that the DNA band had a size of 1500 bp for

amplification using Bact-27F and Uni-1492R primers, and about 250-500 bp

with the Com-IF and Com-2R primers. The sequence of nucleotides was

determined by Sanger sequencing method. The 16S rRNA sequence of the

isolate and other bacteria in GenBank were compared with the Basic Alignment

Search Tool (BLAST) to know the homology of sequence. Then, all of the

sequence were aligned by ClustalW, and the phylogenetic tree was

reconstructed with Neighbor Joining method using bootstrap analysis of 1000

replicates. The results showed that LUI-04 had a homology of 99% and the

percentage bootstrap value of 86% with one strain of Bacillus aryabhattai

B8W22 and three strains of Bacillus megaterium (strain QM B1551,

NBRC15308, and ATCC 14581). Therefore, the LUI-04 bacterium isolate

belongs to Bacillus megaterium species.

Keywords: 16S rRNA gene, phylogenetic tree, potencial probiotic bacteria,

Rastrelliger sp.



OP-D-3

PROFILLING OF SHRIMP SHELL HYDROLYSIS PRODUCT

BY CHITINASE FROM Bacillus licheniformisB2

Maria Goretti M. Purwanto*, Ruth Chrisnasari, Nalista Jayanthi




Abstract

The application of chitin in the area of food and biomedicine is limited due to

its insolubility in aqueous solvents. However, its hydrolysis product, N-acetyl

chitooligosaccharides, is water- and acid- soluble. Bacillus licheniformis B2

isolated in previous study from Ijen Crater, Banyuwangi, East Java, appeared to

be potential as chitin degrader bacteria. The aim of this study was to

characterize the products of shrimp shell chitin degradation by chitinase from

B. licheniformis B2. The hydrolysis products were analyzed using High

Performance Liquid Chromatography (HPLC) with Zorbax Carbohydrate

column and acetonitrile:water (70:30) as the isocratic mobile phase. Two major

signals were detected at retention time of ±5.9 and ±7.8 minutes, assumed come

from N-acetyl Glucosamine (C8H15NO6) and its dimer, N-acetyl

chitooligosaccharides (C16H28N2O11), respectively. This study also showed that

the hydrolysis product has antioxidant activity (57,.29-58.85%) with no

antimicrobial activity. This data may contribute to the production of N-acetyl

Glucosamine (C8H15NO6) and dimer N-acetyl chitooligosaccharides

(C16H28N2O11) from shrimp shell especially for nutraceticals.

Keywords: Bacillus licheniformis B2, chitin, chitinase, N-acetyl

chitooligosaccharide



OP-D-4

Production, Purification and Characterization of Chitinase from

Bacillus licheniformis D11

Adelia Angelin Anastasia, Ruth Chrisnasari*, Aime Clorinda Tapatfeto,

Yonatan Imanuel Krisnajayadi, Tjandra Pantjajani, Maria Goretti Marianti

Purwanto

Department of Biology, Faculty of Biotechnology, Surabaya University,

Raya Kalirungkut, Surabaya 60292, Indonesia *Corresponding author: ruth_c @staff.ubaya.ac.id

Abstract

Thermostable chitinase is an important enzyme in the utilization of shellfish

wastes to solves environmental problem. In this experiment, the enzyme

produced by Bacillus licheniformis D11 that was isolated from Cangar hot

spring in East Java. The enzyme was purified and its kinetic characterized

afterwards. Optimum production of chitinase was obtained when using 0,9 % of

colloidal chitin and culture medium with pH 7 at 52 oC for 30 hours. The

produced chitinase hydrolize chitin efficiently at 59 oC and pH 7. The enzyme

residual activity was 64 %, 44 %, 48 %, 41 % and 31 % after incubated for 2

hours at 50 oC, 60 oC, 70 oC, 80 oC and 90 oC respectively, which proof

thermostability of this enzyme. The crude extract then was purified by

ultrafiltration followed by GFC using Sephadex G-100 resulting 29.86 % of

yield and 193.81 fold of purity enhancement. Highest activity of GFC fraction

was visualized by Sodium Dodecyl Sulfate-Polyacrilamide Gel Electrophoresis

(SDS-PAGE) and showed three bands with molecular weight of 54 kDa, 58

kDa and 69 kDa. The same characteristic of GFC fraction was collected to be

analyze its kinetic activity. The kinetic activity curve demonstrated an allosteric

regulation of chitinase activity. Hence, Hill analysis of binding data performed,

that reveal chitinase had a positive cooperativity (n value > 1) with chitin when

Na+, K+ and Mg2+ ions added.

Keywords: enzyme kinetics, characterization, chitinase, purification, Bacillus

licheniformis



OP-D-5

PURIFICATION AND CHARACTERIZATION OF CHITINASE

FROM Paenibacillus sp.

Maria Fidelia Susanto, Ruth Chrisnasari*, Maria Goretti Marianti Purwanto



*Corresponding author: ruth_c @staff.ubaya.ac.id

Abstract

Chitin is the second most abundant polymer in the world. By using enzymatic

process of chitinase, chitin can be degraded into its derivates which have many

bioactive functions. In this project, Paenibacillus sp. isolated from Prataan hot

spring in Tuban, Indonesia is used as a chitinase producing bacterium.

Chitinase from this isolate is shown to be thermostable. To produce chitinase,

0.9% of colloidal chitin from shrimp shells in thermus broth was used as

substrate for Paenibacillus sp. The fermentation process was run for 36 hours at

48 C. The agitation used was 200 rpm, with 3 vvm of aeration. The crude

chitinase was harvested using centrifugation from fermentation broth. The

supernatant then purified using ultra-filtration membrane followed by Ion

Exchange Chromatography (IEC). The purified protein size was determined

using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-

PAGE). The purified chitinase was also being characterized for enzyme

kinetics activity using various concentrations of colloidal chitin and metal ion

(Mg2+ and K+) addition. The result of this study showed that the purified

chitinase was obtained between fraction number 15 and 20 during the IEC

process and sized around 65 kDa. The purification process yielded 0.48% of

chitinase and the chitinase was purer 1139.42 times compared to the crude

extract. The enzyme demonstrated an allosteric regulation and the substrates

showed positive coordination to enzyme’s binding sites, with the added metal

ion worked as inhibitors.

Keywords: Paenibacillus sp., chitinase, purification, enzyme kinetics,

allosteric enzyme, positive cooperativity



OP-D-6

Rapid reversed-phase high performance liquid chromatography

assay of tert-Butylhydroquinone content in food products

Steven Sutanto1*and Maria Goretti Marianti Purwanto1

1Faculty of Biotechnology, University of Surabaya, Jalan Raya Kalirungkut,

Surabaya 60292, East Java, Indonesia


Abstract

The use of antioxidant has been widely applied in food industry to maintain the

product’s condition to the end consumer. Tert-Butylhydroquinone (TBHQ) is a

synthetic aromatic organic compound which is often used as antioxidant in food,

especially oil product and its derivatives. The use of TBHQ in food products has

been limited by the government in most countries, including Indonesia.

Therefore, the need for a rapid method to assay the concentration of TBHQ in

products is quite high in food industry. In this article, we propose a rapid

reversed-phase HPLC method to assay TBHQ content in food products. The

isocratic system using 1% acetic acid and acetonitrile in 3:2 ratio with 1 mL.min-

1 flow rate produce good separation of TBHQ. Target peak was eluted in 5

minutes and the whole procedure only need maximum of 18 minutes to elute all

the sample component. The LOD of the method was determined at 0.2 mg/L,

while the lower limit of quantification was 0.5 mg/L. The accuracy of the

method has been proved with acceptable recovery ranging from 95% to 105%.

Keywords: antioxidant, HPLC, reversed-phase, TBHQ




OP-D-7

CHARACTERIZATION OF THERMOSTABLE CHITINASE

FROM Bacillus licheniformis B2

Nalista Jayanthi, Maria Goretti M. Purwanto*, Ruth Chrisnasari, Tjandra

Pantjajani, Alicia Wahjudi, Monica Sugiarto




Abstract

Chitinases is an enzyme capable of degrading chitin into oligomers to produce

chitin derivatives products which are more useful. Chitinase was obtained from

Bacillus licheniformis B2 isolated from Ijen hot spring, East Java. The chitinase

has the best chitinolytic activity at pH 7 when colloidal chitin was used as

substrate. The enzyme exhibited activity in broad temperature range, from 50 to

70°C, with optimum at 55°C. It was stable at 50°C until 90 minutes, at

60°Cuntil 60 minutes and at 70°C until 30 minutes. At neutral pH this enzyme

has negative charge but further purification is needed to determine its pI. The

apparent Km and Vmax of this chitinase for colloidal chitin were 101.96 mg/mL

and 2.72 μmol/min.mL. Addition of NaCl, KNO3 and MgSO4 decreased the

activity of chitinase following mixed inhibitor mode. Additionaly, the highest

degradation rate was observed within 30 minutes at 50 mg/mL substrate

concentration. This enzyme should be a good candidate for applications in the

recycling of chitin waste.

Keywords: Bacillus licheniformis B2, characterization, chitinase



OP-D-8

The Isolation and Screening of Proteolytic Bacteria from Sidoarjo

Shrimp Paste as

Protease Source to Extract the Collagen Protein of Milkfish Scales

Evi Susanti*, Suharti, Naharotul Chusniyah dan Shindy Tirta Ayu Paramitha

Department of Chemistry, Faculty of Mathematics and Natural Sciences,

Universitas Negeri Malang,

Jalan Semarang 05, Malang-East Java Province, Indonesia


Abstract

This research aimed to obtain proteolytic bacteria from shrimp pasteSidoarjo

which can be produce protease to be used for collagen extraction from milkfish

scales. The study were consist of proteolytic bacteria isolation, selection of

potential isolate, purification of potential isolates, confirmation of proteolytic

index value of selected isolates, production of crude extract of protease and its

ability test for collagen extraction from milkfish scales. The selective medium

was used in this study skimmed milk agar (SMA) and the production medium

was salt medium with peptone as a source of nitrogen. Enzyme activity was

measured using casein as substrate. The protein concentration was determined

by Lowry method. The ability of collagen extraction was measure based on the

increaseof soluble protein resulted from hydrolysis of milkfish scales bycrude

extract of protease. There were 18 isolates of proteolytic bacteria of shrimp

paste Sidoarjo. Isolate of TR-10, TR-4.1.1 and TR-15.1 were produced high

proteolytic with proteolytic index value of 3.00, 3.23, and 4.00. Isolate of TR-

10 and TR-15.1 produced high activity of proteases in production medium,

respectively 1.07 U/mL and 0.26 U/mL, but only crude extract of protease of

TR-10 that can be used to extract the collagen of milkfish scales as much as

91,1 µg protein per mg milkfish scales.

Keywords: Collagen, milkfish scales, proteolytic bacteria, shrimp paste



OP-D-9

A New Specific Primer for Detection of Pig genome by Quantitative

Polymerase Chain Reaction

Mariana Wahjudi*, Stanley Evander Emeltan Tjoa, Nalista Jayanthi, Natasha

Amanda, Calvin Wijaya, Ruth Chrisnasari

Laboratorium Purifikasi dan Biologi Molekuler, Fakultas Teknobiologi,

Universitas Surabaya, Jl Raya Kalirungkut, Surabaya

*Corresponding author:[email protected]

Abctract

Detection of non-halal food is important in food certification. One of DNA-

based methods for halal detection is quantitative polymerase chain reaction (q-

PCR) method. Several q-PCR primers pairs for pork detection have been

developed. In silico study revealed that these primers have significant similarity

to the genome sequence of animals other than pigs. Therefore, it is necessary to

design a specific primer that will not give any false positive to food stuffs

others than pigs. This study aims to design a specific q-PCR primers for all sub-

ordo suina sequences but not to other animal genomes and to evaluate the

specificity and sensitivity of the primer pair in q-PCR reaction. The result

showed that the MWb primers pair amplified the target sequence of pig genome

with amplicon size of ~219 bp. On annealing temperature of 57 ºC and primer

concentration of 0,05 µM, the limit detection of MWb primer is as low as 0,1

ng of total DNA. No amplicon is detected using cow genome as a template. The

q-PCR efficiency using MWb is higher compared to three others pig primers

pairs. It can be concluded that the new designed primer can be used as good

primers candidate for non-halal ingredients contamination of sub-ordo Suina

animals.

Keywords: Halal, MWb primers, q-PCR, Suina




OP-D-10

The method growing tubular Acetobacter xylinum on the solid

media as precursor of sausage casing

Salafudin1*, Y Pratama2, A H Putri1, S D Rahmatya1 and M. Mel3

1)Department of Chemical Engineering, Institut Teknologi Nasional, Jl. PKH.

Mustopha No.23, Cibeunying Kaler, Bandung 40124, Indonesia 2)Department of Enviromental Engineering, Institut Teknologi Nasional Jl.

PKH. Mustopha No.23, Cibeunying Kaler, Bandung 40124, Indonesia 3)Departement of Biotechnology, Faculty of Engineering, International Islamic

University Malaysia, Gombak, 50728 Kuala Lumpur, Malaysia


Abstract

Nata de coco is well known food in tropical countries which is made from

coconut water waste using Acetobacter xylinum bacteria. The method of

growing A xylinum in tubular form was studied with solid media. The tubular

bio-film is used as an precursor alternative of edible sausage casing. Jelly solid

media was made from coconut water waste, glucose, nutrient, and agarose

powder. All ingredients was mixed and boiled, put in to tubular plastic. The

variable studied ware glucose concentration (10% to 16%), duration of reaction

(10 d to 16 d), and inoculation methods. The inoculation methods were scatting

all surface of tubular jelly with ose needle, submersing the tubular jelly in A

xylinum solution, and putting A xylinum in to warm agarose solution before the

agarose solution became jelly. The experiment was done in an incubator

camber which supplied with pure oxygen regularly. The measurement of

parameters in the research involved COD, TPC, glucose level, and appearance

of biomembrane. The results showed that the most optimum result based on the

treatment method that is on the treatment of scratch while based on glucose

variation of the most optimum result obtained at glucose level 10% and based

on fermentation time the most optimum results showed on the 10th day of

fermentation.

Keywords: Edible sausage casing, fermentation, glucose, nata de coco



POSTER PRESENTER



MEDICAL & FORENSICS



PP-A-1

Antibacterial Activity Roll On Deodorant WithPlucheaindica (L.)

Leaf Extract Against Staphylococcusepidermidis in-Vitro

Oom Komala*1, Ike Yulia W 2, and Nian Rizqiyana2

1Department of Biology, Faculty of Mathematics and Natural Sciences,

Pakuan University

2Department of Pharmacy, Faculty of Mathematics and Natural Sciences,

Pakuan University


Abstract

Plucheaindica L. is a medicinal plant that is often used to eliminate body odor.

This research aims to test effectiveness Roll on deodorant as an antibacterial

against Staphylococcusepidermidis and it’s stability test. Test of Antibacterial

activity used dilution method. Phytochemical analysis for knowing the presence

secondary metabolites on extract. Roll on Deodorant test for 4 types stored at

28-30⁰C for 8 weeks includes observation of the color, odour and homogeneity,

irritation test, pH test, viscosity and specific gravity. The results showed

Plucheaindica (L.) leaf extract’s Roll On Deodorant have antibacterial effect

against Staphylococcusepidermidis. Pluchea indica L. ethanol extract have

bioactive compounds consisting of flavonoids, saponins, tannin and alcaloid.

Roll On Deodorant stored on 28-30⁰C for 8 weeks showed stable for color,

odour and homogeneity. Deodorant roll on have a pH of 4.52 - 5.53, viscosity

331.8 - 340.8 cP, specific gravity 1.0107 - 1.0397 g / ml, and is not cause

irritation.

Keywords: Antibacterial, deodorant roll on, Plucheaindica L



PP-A-2

Formulation of Padina australis Extract Tablet as Antibacterial

Escherichia coli

Tri Saptari Haryani*, Bina Lohita Sari, Triastinurmiatiningsih, Dwi Liana

Hanura1

1University of Pakuan, Bogor


Abstract Padina australis (P.australis) is one of the seaweed that have a potential as

antibacterial Escherichiacoli (E.coli). Triterpenoids and steroids are secondary

metabolites compounds contained in seaweed, and has a variety of activities as

bactericide, and hypo-allergenic. This study aims to establish tablet formulation

and determine the effectiveness against of E.coli bacteria causing diarrheal

disease. The formulation was prepared with these treatment: formula I (20%),

formula II (25%), formula III (30%), using a negative control in the form of

tablet with no active ingredient, and a positive control using amoxycillin (20

ppm). The formulas’ effectiveness was tested by calculating the inhibition zone

width (IZW) –a clear zone resulting from the three formulas, negative, and

positive control– using Kirby-Bauer method. The result shows that the

formulation with active ingredient of 30% of P. australis extract has an

inhibitory activity of 7.7 mm and effective as antibacterial Escherichia coli.

Thus it can be concluded that the formulation of 30% of P. australis extract

tablet is the most effective formula as antibacterial E. coli causing diarrhea

disease.

Keywords: Padina australis, tablets, antibacterial, Escherichia coli.



PP-A-3

THE POTENTIAL OF PACITAN ORANGE (Citrus sinensis)

PEEL EXTRACT AS A STIMULUS OF REGENERATION

CELLS IN

Rattus Norvegicus BURNS

Rinza Rahmawati Samsudin*1 dan Anindita Riesti Retno Arimurti*2

1* Universitas Muhammadiyah Surabaya, Jl. Sutorejo No. 59, Surabaya 2*Universitas Muhammadiyah Surabaya, Jl. Sutorejo No. 59, Surabaya


Abstract Burns are damage to tissues that occur on the surface of the skin. Erythema is

an inflammatory response that first appears in the area of inflammation during

the healing process of burns. The faster the degree of erythema decreases, the

faster the healing process for burns. Pacitan orange peel extract contains d-

limonen active compounds, flavonoids, saponins, and tannins which can

stimulate cell regeneration in burns healing. The purpose of this study was to

determine the effect of extracting Pacitan orange peel for the reducing of

erythema in Rattus norvegicus healing burns degree II. This research is an

experimental. The research utilize 25 white rats divided into 5 different groups

treatment. In K0 as a control, white rats that experienced burns degree II were

only given aquadest, K1 was given burn treatment using normal saline, K2 was

treated with 40% Pacitan orange peel extract, K3 was treated with burns using

60% Pacitan orange peel extract and K4 given burn treatment using 80%

Pacitan orange peel extract. The treatment was carried out for 7 days. The

obtained data from the research were then analyzed by Parametric Test One

Way Analysis of Variance (ANOVA). Based on Homogeneity test by

processing data using the Levene test, it is known that the significance value is

0.829> 0.05, so it can be concluded that the data is homogeneous. Whereas for

the data processing normality test using Shapiro-Wilk test where the value of

P> 0.05, it can be concluded that data data are normally distributed. The results

showed that the allotment of Pacitan orange peel extract for 7 days could

accelerate the reduction of erythema, significantly with a P <0.05.

Keywords: Pacitan orange peel extract (Citrus sinensis), burns, Rattus

norvegicus.




PP-A-4

CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY

TEST OF SIMBION SPONGE BACTERIA AGAINST

PATHOGENIC BACTERIA

EndangSulistyarini Gultom1) and Hasruddin2) 1) Biology Lecturer at FMIPA, Medan State University, Willem Iskandar Pasar

V Street, Medan Estate 2) Biology Lecturer at FMIPA, Medan State University, Willem Iskandar Pasar

V Street, Medan Estate.


Abstract This study aims to characterize and explore the potential antibacterial sponge

symbion bacteria from Ngge Island waters, Sibolga. The steps taken were

isolation of sponge symbion bacteria, characterization of sponge symbiotic

bacteria isolates, and testing of sponge symbiotic antibacterial activity against

Staphylococcus aureus, Eschericia coli, and Salmonella typhi. Based on the

research, 13 sponge symbiotic bacterial isolates were obtained from the third

mesohyl sponge, namely 5 bacterial isolates from Haliclona sp., 4 bacterial

isolates from Clathiria sp., and 4 bacterial isolates from Callyspongiasp.

Morphological characterization results of sponge symbiotic bacterial colonies

were obtained in a circular form on all sponge symbiotic bacterial isolates, with

elevations occurring in 10 isolates, hilly in 2 isolates, convex in 1 isolate and

bank in sponge symbionic isolates, 12 isolates (slippery) and 1 isolate (choppy).

Based on gram staining obtained 11 gram-negative bacterial isolates, 2 gram-

positive bacterial isolates, 10 bacillary isolates and 3 coccus-shaped isolates.

Antibacterial potential test results for Staphylococcus aureus bacteria,

Eschericia coli, and Salmonella typhii, obtained 9 potential sponge symbiotic

bacterial isolates, with inhibition zones of 11 mm, 9 mm and 11 mm

respectively.

Keywords: antibacterial, sponge symbion bacteria, pathogenic bacteria



AGRICULTURE



PP-B-1

Genetic Variability of Soybean Genotypes for Pod Shattering

Resistance using Oven Methods

Ayda Krisnawati* and M. Muchlish Adie

Indonesian Legume and Tuber Crops Research Institute

Jl. Raya Kendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia.

Tel.: +62-341-801468, 801075, Fax: +62-341-801496


Abstract

Consumer demand for soybean is not only high yielding variety, but also must

be in accordance with industrial preferences, primarily for its shattering

resistance. A total of 14 soybean lines were evaluated in Nganjuk from

February to May 2018. Anjasmoro variety was used for pod shattering resistant

check, and Dega 1 as large seeded-size check variety. The pod shattering

evaluation was using oven-dry method. The average seed yield of 16 tested

lines was ranged from 3.15 – 4.06 t/ha, with the range of seed size and days to

maturity were 13.55 – 20.56 g/100 seed and 78 – 84 days, respectively. All

tested lines have large seed size, except SAT-Ng-A4. Five lines have early days

to maturity (<80 days). The highest yielding line and large seeded size was

SAT-Ng-AG-3 (4.06 t/ha), but it has medium maturity. Pod shattering

evaluation which using a tiered system of temperature showed that oven

temperature of 50° and 60°C were able to differentiate the soybean resistance to

pod shattering. Three very resistant lines and one resistant line were obtained

after exposed to 60°C. The soybean pods which allowed to dry at room

temperature for six days and exposed to high temperature (80°C) showed high

variability on shattering resistant. Three resistant lines (SAT-Ng-A4, SAT-Ng-

6-13, and SAT-Ng-5-5) were obtained from high temperature method, as well

as from a tiered oven temperature method. Those three lines with high yield,

large seed size, and medium maturity can be used as parental lines to improve

soybean shattering resistance in the breeding program.

Keywords: oven method, pod shattering, soybean, yield.



PP-B-2

Characterization of Adaptive and Productive Soybean Genotypes in

Dry Land of Kalimantan, Indonesia.

M. Muchlish Adie*1, Ayda Krisnawati1, and Dotti Suryati2

1Indonesian Legume and Tuber Crops Research Institute

Jl. Raya Kendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. 2Faculty of Agriculture, University of Bengkulu


Abstract

Dry land is one of potential areas for soybean development. A total of 12

soybean genotypes, including two check varieties of Anjasmoro (high yield)

and Demas 1 (adaptive in acidic dry land) were evaluated in two locations of

dry land in Gunung Makmur Village and Kuala Tambangan Village (Takisung

District, Tanah Laut Regency) from May to August 2017. The experimental

design in each location was randomized complete block design with four

replicates. Fertilizer consisted of 250 kg Phonska, 100 kg SP36, and 500 kg/ha

dolomite, which applied entirely during sowing time. Plant and disease were

controlled optimally. The location of Gunung Makmur has pH H2O of 6.1 and

pH KCl of 4.2, whereas Tambangan has pH H2O of 5.8 and pH KCl of 5.0. The

range of seed yield in Gunung Makmur was 1.56 – 3.18 t/ha and in Tambangan

was 1.72 – 2.96 t/ha. Anjasmoro variety showed a higher productivity than

Demas 1. Genotype of 19BE produced the highest yield in Gunung Makmur

(3.18 t/ha), meawhile G511H/Anjs-1-3 produced the highest yield in

Nambangan (2.96 t/ha). The decline in soil pH was in line with decline in seed

yield. The best genotype across environments was G511H/Anjs-1-3 (2.99 t/ha),

followed by 19BE (2.74 t/ha). The agronomic characters of G511H/Anjs-1-3

was medium height and a relatively low of empty pods, meanwhile 19BE has

medium height and relatively high numbers of nodes per plant and branches per

plant. Those genotypes could be developed in acidic dry land area of

Kalimantan.

Keywords: agronomic characters, dry land, seed yield, soybean.



PP-B-3

The performance of three local rice (Oryza sativa) cultivar from

East Kalimantan under osmotic stress at early seedling stage

Ida Bagus Made Artadana, Ines Tiara Dewi, Johan Sukweenadhi




Abstract

Climate change made rain fall unpredictable leading to sudden drought stress

during crop cultivation period. Osmotic stress induced by drought stress affect

the crop growth and development especially during seedling period. This

research aims to evaluate the performance of three local rice seedling under

osmotic stress induced by PEG 6000. Three local rice cultivars were

germinated and then divided into three groups. Three days after germination,

each group were treated with either 0%, 15% or 25% PEG 6000 solution for 10

days. Osmotic stress induced by PEG significantly reduced plant growth and

total chlorophyll content in the leaf. Among of the three cultivars, Mayas Putih

was the most sensitive to the exposure of osmotic stress for 10 days at seedling

stage. Additionally, osmotic stress induced increasing of soluble sugar in the

leaf of all rice cultivars. This may indicate that soluble sugar play a role as

osmoprotectant under osmotic stress in those three cultivars.

Keywords: Osmotic stress, Rice seedling, Mayas Putih, Soluble sugar



PP-B-4

Production potensial of sweet corn (Zea mays Linn. var.

saccharata) to different planting pattern and P sources

PG Adinurani1*, S Rahayu1, L S Budi1, S Pambudi1 and P Soni2

1)Faculty of Agriculture, Merdeka University of Madiun, Jl. Serayu No.79,

Pandean, Madiun 63133, Indonesia 2)Department of Agricultural and Food Engineering, Indian Institute of

Technology Kharagpur, Kharagpur, West Bengal 721302, India


Abstract

Planting pattern factors that regulate population density of plant greatly

determine optimal plant growth and production. The “Jajar legowo” system

adopted from rice plants was applied to corn plants to obtain the effect of

marginal plants. The study wanted to know sweet corn production based on

planting patterns on various sources P. The study used factorial experiments

which were arranged based on a randomized block design which was repeated

three times with the planting pattern factor including conventional planting 70

cm x 20 cm, “Jajar Legowo” (2:1) with 20 cm x 50 cm x 100 cm and dense

planting 105 cm x 12.5 cm. Source factor P consists of SP 36, mycorrhiza plus,

and biofertilizer. The results showed that there was a significant interaction

effect between the planting pattern and source of P on the wet weight and the

dry weight of the plant, mostly in the combination treatment of the

conventional system with SP 36 are 555 g and 123.51 g respectively. The

weight corn cobs with and without leaf have the highest weight on the dense

planting pattern which is no different significantly from conventional planting

pattern.

Keywords: biofertilizer, legowo row, marginal land, mycorrhiza, SP-36



PP-B-5

Local variety of “Uwi” (Dioscorea alata Linn.) plant in west region

of East Java upland

Wuryantoro1, R I Fitriyani1 and P Soni2

1)Faculty of Agriculture,Merdeka University of Madiun, Jl. Serayu No.79,

Pandean, Madiun 63133, Indonesia 2)Department of Agricultural and Food Engineering, Indian Institute of

Technology Kharagpur, Kharagpur, West Bengal 721302, India


Abstract

Diversification efforts through empowerment of upland is a necessity for food

security in the future. “Uwi” are very tolerant of various agro-climates and

have potential genetic variations for different functional foods. The survey

obtained 45 plant accession for testing in a controlled environment. Cluster

analysis is used to trace the specific appearance of the accession based on the

morphology of tubers. There was four groups of "uwi" with70 % similarity

level. The first group spread in the C2, C3 and B2 agro-climates, the second

group found in C2, the third group spread in C2, and C3, fourth group

spreads in the C2, C3 and D3. The number of accessions analysis, 14 were

found in the type C2, 32 were in the C3, two spread in agro-climate B2 and

one was obtained in D3. Concluded that "uwi" plants have broad tolerance for

many agro-climate so that they have the potential to be produced on a large

scale to obtain alternative food and functional. Specific features of differences

include the colour of tuber, the form of tubers, the taste of tubers and tuber

fibre. While the similarities are in the general character of the outer skin colour

and the age of the plant.

Keywords: Adaptation, food security, upland, uwi (Dioscorea alata Linn.)



ENVIRONMENT &

ENERGY



PP-C-1

Phytotoxicity and Developmental Effect of CASHEW Nut Shell

Extract Against Nymphal Instar of Whitefly (Bemisiatabaci Genn.)

Wuye Ria Andayanie*1, Wahidin Nuriana1, Netty Ermawati2

1Agriculture Faculty, Merdeka Madiun University, Serayu No 79, Madiun,

63133, East Java, Indonesia 2State Polytechnic of Jember, 68120, East Java, Mastrip, Jember ,68101, East

Java, Indonesia


Abstract

Cashew nut shell (CNS), which are one of the most still is tropical biomass

waste. This study was aimed to test the mortality rates of CNS extract against

young nymphs (first and second instar) and old nymphs (third instar). The

concentration of CNS extractswere: 0.22 %, 0.67%, 2.00 %, 6.00%. These The

design of experiment was completely randomized with four treatments, and

four replications. The result experiments indicated that the LD50 values of

CNS extract at concentration of 0.22%, 0.67%, 2.00 %, 6.00% against younger

nymphs were 0.11, 0.09, 0.24 and 0.13, respectively. Whereas the LD90 values

of the same concentration of CNS extract were 0.37, 0.50, 1.59 and 0.65 %,

respectively. The mortality of older nymphs required high rates concentration

of CNS extract. The LD50 values of CNS extract at concentration of 0.22 %,

0.67%, 2.00%, 6.00% against older nymphs were 0.86, 1.01, 1.14, 2.11 %,

respectively. However, the LD90 values of them were 3.23, 5.78, 5.33, 3.80%,

respectively. These CNS extract could be used to suppress whitefly population.

Keywords: whitefly, cashew nut shell, nymphs, mortality rates



PP-C-2

Applying species distribution models to identify the conservation

area by area habitat suitability: A case study using Zanthoxyluma

canthopodium DC.

Cicik Suriani*, Tri Harsono, Dina Handayani, Eko Prasetya

Departement of Biology, Universitas Negeri Medan. Jl. Willem Iskandar, Pasar

V, Medan Estate, Medan 20221, North Sumatra, Indonesia


Abstract

Zanthoxyluma canthopodium DC is a member of the Rutaceae family which is

spread in the northern part of Sumatra, Indonesia. This plant is very closely

related to Batak ethnic culture. This study aims to analyze conservation areas in

accordance with habitat suitability using the species distribution modeling

approach. The results of field exploration were obtained 158 points from 9

regencies in North Sumatra. The results showed that most of the Z.

acanthopodium was spread on the highlands around Lake Toba. Andaliman

mostly occupies an area with elevations of 1500-2000 meters with rainfall

between 2000-2500 mm/year. Zanthoxyluma canthopodium occupies a narrow

habitat including 6 types of land cover namely dry land agricultural land, open

land, settlements, secondary dryland forests, rice fields, and shrubs. Most of

these are found in dry land farming areas. Most of Andaliman were found in

acrisols and a small part in orthic podzols, cambisols, and ferrasols. This

research can be used as initial information in the cultivation and conservation

of Z. acanthopodium.

Keywords: Zanthoxyluma canthopodium, North Sumatera, Species

Distribution

Modelling, Habitat Suitability Area, ArcGis.



PP-C-3

An engineering of floating net aquaculture system of Tiger Grouper

[Ephinephelus fuscoguttatus (Forsskal, 1775)] on suplementation

Bacillus subtilis (Ehrenberg, 1835) probiotic in the diet

D Rachmawati1*, R H Setyobudi2 and Z Vincevica-Gaile3

1)Aquaculture Departement, Faculty of Fisheries and Marine Science,

Diponegoro University, Jl.Prof.H.Soedarto SH, Tembalang, Semarang 50275,

Indonesia

2)Postgraduate University Muhamadyah of Malang, Jl. Raya Tlogomas No.

246 Malang 65114, Indonesia

3)Department of Environmental Science, University of Latvia, Raina Blvd. 19,

Riga, LV-1586, Latvia, European Union

Corresponding author: dianarachmawati1964@gmail

Abstract

An engineering of floating net aquaculture system of Tiger Grouper

[Ephinephelus fuscoguttatus (Forsskål, 1775)] on Bacillus subtilis (Ehrenberg,

1835) probiotic suplementation in the diet is one of the solutions to overcome

the deteriotion of the aquaculture environment that is caused by the

accumulation of dieting waste. The purpose of the research was to study the

effects of B. subtilis probiotic suplementation in the diet on protein

digestibility, efficiency of diet utilization, growth, activities of digesting

enzymes, and blood profile of E. fuscoguttatus. The experimental fish was

raised in the floating net aquaculture. The sampled fish has an average weight

of 4.24 g ± 0.023 g per fish. Diet used in the study contained 45 % protein

with the suplementation of various amounts of B. subtilis probiotic. The

amounts were 0 % per kg diet (A treatment), 5 % per kg diet (B treatment), 10

% per kg diet (C treatment), and 15 % per kg diet (D treatment). The results

show that B. subtilis probiotic suplementation in the diet significantly affected

on protein digestibility (ADCP), efficiency of diet utilization (EFU), feed

conversion ratio (FCR), protein efficiency ratio (PER), relative growth rate

(RGR), survival rate (SR), and activities of digesting enzymes; however, it did

not significantly influence blood profile of E. fuscoguttatus. C treatment with

the B. subtilis probiotic suplementation dose of 10 % per kg diet was the best

dose. The optimum doses of B. subtilis probiotic addition for ADCP, EFU,

FCR, PER, RGR and SR were (9.42, 9.42, 10.00, 9.63, 9.63, 9.48) % per kg

diet respectively.

Keywords: Blood profile, diet utilization, digesting enzymes, growth,

protein digestibility



PP-C-4

Performance UNEJ #1 stove low energy using bio-pellet from coffee

husk

S S Harsono1, M B N Prayogo1, Razhika1, R H Setyobudi2 and P Soni3

1)Departement of Agricultural Engineering, Faculty of Agricultural Technology

University of Jember, Jl. Kalimantan No. 27, Jember 68121, Indonesia 2)Waste Laboratory, University Muhamadyah of Malang, Jl. Raya Tlogomas

No. 246 Malang 65114, Indonesia 3)Departement of Food, Agriculture and Bioresources, School of Environment,

Resources and Development, Asian Institute of Technology, PO Box 4, Klong

Luang Pathumthani 12120, Thailand


Abstract

The level of fuel consumption especially of fossil fuels will increase along with

increasing human population and the rising rate of the industry in many

countries. There will be causing a fuel crisis especially fuel oil and gas if this

happens. Bio-pellet from coffee husk is a renewable energy substitute for oil

which is environmentally friendly. Performance test of coffee husk biopellet as

a source for UNEJ#1 biomass stove was carriout.The variable include heat,

thermal efficiency, and emissions, with UB-03 stove as control.. The result

shows that heat on 40 holes of UNEJ#1 stove is 316 880 kJ, 20 holes of

UNEJ#1 stove is 310 601 kJ, 10 holes UNEJ#1 stove is 308 229 kJ, and UB-03

stove is 306 833 kJ. Thermal efficiency of UB-03 stove is 16.47 %, 10 holes of

UNEJ#1 stove is 16.39 %, 20 holes of UNEJ#1 stove is 15.96 %, and 40 holes

of UNEJ#1 stove is 15.38 %. Emissions result of UB-03 stove is 333 mg kg–1,

10 holes UNEJ#1stove is 298 mg kg–1, 20 holes of UNEJ#1 stove is 289 mg kg–

1, and 40 holes of UNEJ#1 stove is 273 mg kg–1.

Keywords: Bio refinery, emission, renewable energy, coffee solid waste,

thermal efficiency



PP-C-5

Fish diversity in Cimanuk river before and after rainy season in

Jatigede reservoir, West Java, Indonesia

T Herawati1*, AYustiati1, I Adiwiguna2,IRostini1A Sahidin1

and H Juahir 3

1)Aquaculture Departement, Faculty of Fisheries and Marine Science,

Universitas Padjadjaran, Jl.Raya Bandung Sumedang Km. 21, Jatinangor45363,

Indonesia 2)Alumnus Faculty of Fisheries and Marine Science, Universitas Padjadjaran,

Jl.Raya Bandung Sumedang Km. 21,

Jatinangor 45363, Indonesia. 3)East Coast Environmental Research Institut -Sultan Zainal Abidin University,

Gong Badak, 21300 Kuala Terengganu, Malaysia *Corresponding author: [email protected]

Abstract.

The purpose of the research was to map the diversity of fish living in the

Cimanuk River before and after the Jatigede Reservoir. Research using

field observation method, and census sampling technique. Research is

done in the rainy season in November 2017 until January 2018, fish

taken from four stations. Parameters measured by the diversity index,

dominance index, equitability index, and water quality. Data analysis

used a comparative descriptive method by mapping the species and

comparing the diversity of fish before and after the Jatigede Dam. The

results of the research indicate that the identified fishes are six families

consisting of 14 species, the diversity of Cimanuk River fish before

Jatigede Reservoir (station I and II), including moderate diversity

1.735≤H'≤1.909, low species dominance 0.185≤C≤0.194, high

equitability 0.61≤E≤0.789, stable fish community structure. Diversity of

fish after Jatigede Reservoir (station III and IV), including low with

0.759≤H'≤0.855, moderate species dominance 0,55≤C≤0.57, low

equitability 0.296≤E≤ 0.317, distressed fish community structure. Water

quality in general meets the requirements of water quality standards for

fisheries, except for light penetration, nitrite at station I, IV and

ammonia at station III that has exceeded environmental quality

standards, but can still be tolerated by several types of fish.




Keywords: Diversity, environmental, equitability, fish map, water

quality



PP-C-6

The Fe non-heme prospectin coffee flour made from coffee solid

waste: Mini review

R H Setyobudi1,*, L Zalizar2, S K Wahono3,4, S S Harsono5, P G

Adinurani6, J Burlakovs7, Y A Nugroho8 and T Liwang8

1)Waste Laboratory of University Muhamadiyah of Malang, Jl. Raya

Tlogomas No. 246, Malang 65114, Indonesia 2)Faculty of Agricultural and Animal Science, University Muhamadiyah

of Malang, Jl. Raya Tlogomas No. 246, Malang65114, Indonesia 3)Future Industry Institute and School of Engineering, University of South

Australia, Mawson Lakes SA 5095, Adelaide, Australia. 4)Technical Implementation Unit for Development of Chemical

Engineering Processes, Indonesian Institute of Sciences, 55861,

Yogyakarta, Indonesia 5)Faculty of Agricultural Technology University of Jember, Departement

of Agricultural Engineering, Jl. Kalimantan No. 27,

Jember 68121, Indonesia 6)Faculty of Agrotechnology, Merdeka University of Madiun,

Jl. Serayu No. 79 Madiun 63131, Indonesia 7)Faculty of Health and Life Sciences, Departemen of Biology and

Environmental Science, Linnaeus University, 391 82 Kalmar/ 351 95

Växjö, Sweden 8)Plant Production and Biotechnology Division, PT Sinarmas

Agroresources and Technology Tbk., Sinar Mas Land Plaza, 2nd Tower

10th Fl. Jl. M.H. Thmrin No. 51, Jakarta 10350, Indonesia

Abstract.

Coffee flour (CF) from coffee pulp or husk,solid waste of coffee

processing have launched in Canada since 2014. This product is claimed

as certified of gluten-free, vegan, kosher, paleo, and non-GMO.CF is

stated to contain three times Fe content than fresh spinach (Amaranthus

spp.); five times fiber content than wheat flour; 84% fat content lower

and 42% fiber content higher than coconut flour; 38 % anti-oxidant

content higher than pomegranate (Punica granatum); three times protein

content of kale (Brassica oleracea var. sabellica); and two times

potassium content of banana (Musa paradisiaca). Several receipts of

cookies, donuts, and cakes using CF has been introduced as wheat flour

substitution. However, the scientific publication of CF effect for health



does not appear until August 2018 yet. The mini-review research was

conducted to investigate the Fe content in CF. This Fe non-heme

prospect is allegedly unable to be absorbed optimally by the organism.

Coffee pulp and husk contain an inhibitor, such as tannin, caffeine,

phenol, dan calcium which detain Fe absorption. On the other hand, the

promoter/enhancer of Fe absorption such as vitamin C and amino acid

was released in CF processing. Several types of research have to be

conducted to tackle this problem.

Keywords: Biorefinery, Fe inhibitor, Fe promoter, hemoglobin, zero

waste



PP-C-7

Does the utilization of waste as the green energy alternative

economically feasible?

N Syaifudin1,2*, Nurkholis3, R Handika4 and RH Setyobudi5

1UWA Business School, Crawley Campus, The University of Western

Australia, 35 Stirling Highway, 6009 Perth, Australia 2Fiscal Policy Agency, Ministry of Finance of Republic of Indonesia,

Notohamiprodjo Building, 1st Floor, Jl. Wahidin No 1, Senen, Jakarta, 10710,

Indonesia 3Faculty of Economics, University of Indonesia, Economics Department

Building, 1st Floor, Depok, West Java, 16424, Indonesia 4College of Business Administration, Abu Dhabi University, Abu Dhabi

Campus, P.O Box 59911, Abu Dhabi, Uni Arab Emirates 5Waste Laboratory University Muhamadiyah of Malang, Jl. Raya Tlogomas

No. 246 Malang, 65114, Indonesia


Abstract

The high demand for fossil fuel energy is a problem and obstacle for

implementing the Green Economy. The higher the fossil price the higher the

cost burden on production activities, whether for industry or households. On

other hand, the high international price of energy is escalating the significant

burden of the government budget subsidy for fuel and energy prices.

Optimization of waste to be transformed into energy (WtE) can be one

alternative solution to the energy crisis and also to help implement the Green

Economy policy. Optimization of waste treatment has great potential for

providing net benefits. This study particularly will discuss the potential of the

utilization of waste as the alternative green energy. Further, the economic

feasibility study will be conducted to assess the net benefit of the

implementation of the policy to the economy.

Keywords: environmental friendly, subsidized fuel, green economy,

renewable energy, waste to energy




FOOD & NUTRITION



PP-D-1

Preparation and Characterization of Butterfly Pea Color Extract

Encapsulated with Various Coating Agents

Hana Susanti Maleta1, Renny Indrawati*1,2, Rosita Dwi Chandra1

1Ma Chung Research Center of Photosynthetic Pigments, Renny Indrawati,

Universitas Ma Chung, Villa Puncak Tidar N1, Malang, 0341-550171,

081334678375 2Chemistry Study Program, Universitas Ma Chung, Villa Puncak Tidar N1,

Malang, 0341-550171


Abstract

Nowadays, the use of natural pigments in food products has gained much

interest in the society, particularly due to the rise in the number of reported side

effects of synthetic colorants. Anthocyanin, a pigment responsible for red to

purplish blue color, has a potential use as natural food colorants because of its

great solubility in water. Among many kinds of its plants sources, the butterfly

pea (Clitoria ternatea L) color extract exhibits vivid blue color at wide range of

various pH environments. Hence, it is often used as the source of natural blue

colorant. However, the primary obstacle of the use of natural pigments is the

poor stability during food processing. The present study is aimed to prepare and

do the characterization of the color extract of butterfly pea which is

encapsulated with various coating agents. The encapsulating materials were

maltodextrin (MDX), mixture of maltodextrin and sodium alginate (MDX-

NaAlg), and polyvinyl-pyrrolidone (PVP). The color value, anthocyanin

content, antioxidant activity, and encapsulation efficiency weredetermined prior

to stability evaluation. The results showed that the blue anthocyanin coated by

15% of PVP performedhighest antioxidant activity, whereasthe highest

encapsulation efficiency was presented by combination of MDX-NaAlg.

Furthermore, the SEM profiles of each lyophilized powder confirmed its

microstructures.

Keywords: anthocyanin, butterfly pea, antioxidant, encapsulation,

lyophilization.



PP-D-2

Photodynamic Inactivation of Escherichia coli by the Crude

Pigments Extract of Various Tea Dregs

Renny Indrawati*1,2, Jovine Marcella Kurniawan1, Ivanna Gunawan1, Cledwyn

Davian Irsan1, Heriyanto1,2, and Leenawaty Limantara3

1Department of Chemistry, Faculty of Science and Technology, Universitas Ma

Chung, Jl. Villa Puncak Tidar N-1, Malang 65151, Indonesia, 081334678375 2Ma Chung Research Center for Photosynthetic Pigments (MRCPP),

Universitas Ma Chung, Jl. Villa Puncak Tidar N-1, Malang 65151, Indonesia 3Center of Urban Studies, Universitas Pembangunan Jaya, Jl. Cendrawasih,

Sawah Baru, Ciputat, Tangerang Selatan 15413, Indonesia


Abstract

Inactivation of pathogenic microorganism is one of the critical points that must

be ensured during food processing. However, common procedures applied by

food manufacturers are either thermal processes or chemical treatments, which

may impact on nutrient loss or unwanted chemical residue.

Photodynamicmethod for microbial inactivation proposes a non-thermal food

processing with a relative short time, which involves three main components,

i.e. sensitizer agent, light and oxygen, to generate the reactive oxygen species

that induces the death of microbial cell. In fact, the tea dregs contained

significant amount of chlorophylls and its derivatives that possess

photodynamic activity as sensitizers. The aims of the present study were: (i) to

determine the composition of chlorophylls and its derivatives in the tea dregs

of black, green, oolong tea, and (ii) to evaluate the in vitro photodynamic

activity of its crude pigment extracts against Escherichia coli. The tea dregs

were extracted using acetone, and the pigments fractions were identified by

means of thin layer and high performance liquid chromatography. Purified

chlorophyll, pheophytin, and pheophorbide were used as references during the

evaluation of photodynamic activity. The results showed that the dregs of black

and oolong tea containedhigher amount of pheophorbide a and pheophytin a

than that of green tea. The inactivation activity of the crude pigments extracts

of tea dregs against E. coli, compared to the references, was followed the order:

pheophorbide > pheophytin >black tea> oolong tea >green tea> chlorophyll.

Therefore, the tea dregs could be used as rich and inexpensive source of

sensitizing agents for inactivation of pathogenic microorganism through

photodynamic method.

Keywords: chlorophyll derivatives, microbial inactivation, photodynamic,

sensitizer, tea dregs



PP-D-3

The Function of Ethanolic Extract Plectranthus ambioinicus Lour

Spreng as Preventive and Curative Against The Effects of

Rhodamine B in Rats

Melva Silitonga*, Pasar Maulim Silitonga, Martina Restuaty

Universitas Negeri Medan, Medan North Sumatera Indonesia


Abstract

Rhodamin B is a flavor enhancer, is used in modern nutrition to improve food

palatability. The objectives of the current study were to investigate the effect of

ethanolic extract of Plectranthus amboinicus L. Spreng (EEP) as a preventive

and curative to the toxic effects of rhodamine B. Adult male rats were divided

into eight groups: the negative control (CMC), the positive control (980 mg

Rhodamin B)/kg bw, preventive 350, 700 and 1050 mg EEP / kg bw and

curative 350, 700 and 1050 mg EEP / kg bw. EEP and Rhodamin-B was given

by oral gavages. On preventive treatment was done by giving EEP from the 1st

day to 42nd then rhodamin B was given from 21st to 42nd day. Whereas in

curative treatment was done by first inducting rhodamin B from the first day to

the 21st, then EEP is gave from the 22nd day until the 42nd. Parameters were

observed namely body weight, organ ratio, ALT and AST. The data were

analyzed with ANOVA and followed by LSD. The results showed that in

preventive and curative treatment EEP dose 700 mg/kg bw increased body

weight, 1050 mg/kg bw increased ratio liver weight to body weight

significantly (p<0.05). On preventive treatment EEP decreased ALT and AST

significantly, whereas in the curative treatment, not only ALT but also AST

increased significantly in the treatment of Rhodamine-B and all EEP doses

have not been able to reduce the levels of ALT and AST.

Keywords: Plectranthus amboinicus L. Spreng, body weight, ratio liver

weight, ALT, AST



PP-D-4

Potency of Vegetable Oil to Increase Microflora Health Using In

Vitro and In Vivo Evaluation

Nurhayati Nurhayati*, Vika Nurluthfiyani Ni’maturrakhmat, and Yuli Witono

Department of Agricultural Product Technology Faculty of Agricultural

Technology, University of Jember. Jl. Kalimantan No. 37 FTP UNEJ Jember

68121


Abstract Vegetable oil is a food ingredient that is often consumed along with other

foods. Some types of vegetable oils include coconut oil, palm oil, olive oil, corn

oil, soybean oil, sesame oil, etc. These study evaluated the potency of vegetable

oil i.e coconut oil, palm oil, olive oil, corn oil, soybean oil to improve the

microflora human health. In vitro evaluation was based on the ability of

vegetable oil as growing medium for microflora models. In vivo evaluation was

based on the microflora modulation of rat faecal consumed the vegetable oil.

Result of in vitro evaluation showed that coconut oil was better to increase the

probiotic population of microflora up to 2.7%. Olive oil decreased the

population of pathogenic bacteria up to 51.3%. Result of in vivo evaluation

showed that olive oil was more capable to increase the probiotic population up

to 16.5%, while coconut oil was more capable to reduce the pathogen

population up to 5,1%. Corn oil can promote the Wistar rat to eat more the

feed. The water consumption increase by consuming olive oil, but coconut oil

gave the opposite affect.

Keywords: vegetable oil, coconut oil, Wistar rat, probiotic, microflora



PP-D-5

Quality and Consumer Acceptance Improvement on Prime Local

Soy Milk (Glycine max (L) Merrill) Varieties

Elfi Anis Saati*1, Sri Wahyuni2, Sri Winarsih1, Khoiriyah3

1Instructor of Food Science and Technology Program, Agriculture and Animal

Husbandry Faculty, Muhammadiyah University of Malang 2Alumnus of Food Science and Technology Program, Agriculture and Animal

Husbandry Faculty, Muhammadiyah University of Malang


Abstract

Soy milk is a healthy drink that has a lot of consumer. One of soy milk problem

is its off flavor that needs technique to improve the taste. This study aimed to

determine the effect of local soybean varieties, soaking method, and natural

essence use on soy milk quality. Randomized Complete Block Design (RCBD)

factorial, 2 factors were used. The first factor was soybean varieties that

consisted of Argomulyo, Bromo, Wilis, and Anjasmoro. The second factor was

soaking method that comprised of without soaking, soaked in water for 4 hours,

soaked in water for 8 hours, soaked in NaHCO3 0.5% for 15 minutes, soaked in

Ca(OH)2 0.5% for 15 minutes, soaked in water and boiled in Ca(OH)2 0.5% for

15 minutes. Natural essence (3%) added in the best result, repeated twice. The

best treatment of this was Agromulyo soybean soaked in NaHCO3 0.5% for 15

minutes in which soy milk has moisture content 84.54%, protein content

2.67%, total dissolved solids 14.00 0Brix, pH 7.10, no off flavor (2.20 points),

appearance 3.53 points (interesting enough), preference 3.21 points (like

enough), and high in antioxidant (90.74%).

Keywords: Soy milk, prime soy bean varieties, soaking method, natural

essence



PP-D-6

The Utilization of Telang Flower as Healthy-Natural Food Coloring

on Dawet Drink

Elfi Anis Saati*1, Rachmi Diah Mulandari2, Moch. Wachid1, Sri Winarsih1

1Lecturer of Food Science and Technology Department, Agricultural and

Animal Husbandry Faculty, University of Muhammadiyah Malang 2Alumnus of Food Science and Technology Department, Agricultural and

Animal Husbandry Faculty, University of Muhammadiyah Malang


Abstract

The use of coloring in food industry increased. One of natural food coloring

source is flower. Natural blue pigment is hard to get. Anthocyanin is a

flavonoid that dissolved in water, its pigment range are red to blue and easily

found in plants. Anthocyanin is not stable in neutral or base condition, so it

needs to be extracted in acid condition. Telang flower or Clitoria ternatae L. is

one of blue anthocyanin pigment source. Telang flower anthocyanin extract can

be alternative as synthetic food coloring substitute. Simple randomized block

design was used in this study. This study consisted of 2 steps. The first step

was anthocyanin pigment extraction from telang flower using citric acid and

tartaric acid with concentration variation 0.25%, 0.50%, and 0.75%. Extract

total anthocyanin, total sugar, total dissolved solids, pH, color intensity, and

yield were analyzed for the first step. The second step was telang flower

anthocyanin extract application on Dawet and compared to synthetic food

coloring and textile coloring. The best treatment was telang flower anthocyanin

extract and 0.75% tartaric acid (P6) with pH 3.17, total dissolved solids

2.53oBrix, brightness (L) 32.33, redness (a+) 4.17, bluish (b-) 2.27, total

anthocyanin 56.82 mg/ml, yield 39.13%, and anthocyanin decreased level when

applied to dawet 70.78%, but still considered more preferred than dawet with

synthetic food coloring.

Keywords: Anthocyanin, Telang flower, extraction, citric acid, tartaric acid




PP-D-7

Artificial Rice as an Alternative Functional Food to SupportFood

Diversification Program

Erica Valencia and Maria Goretti Marianti Purwanto*

Faculty of Biotechnology, University of Surabaya, Indonesia


Abstract

Functional food is a food that has a beneficial effect to the body beyond its

adequate nutritional effects that can help improve someone’s health and/or

decrease the risk of disease. Awareness of the importance of health that began

to grow in the community encourage the development of functional food. One

of the functional food that has been developed in Indonesia is in the form of

artificial rice. Artificial rice is an alternative paddy rice substitute that has a

minimally equal value to paddy rice. Artificial rice is made to reduce people’s

dependence on paddy rice and to support the food diversification program so

that the food security in Indonesia can be achieved. Artificial rice is made from

a non-paddy rice flour with a certain composition, so it can be used as a

vehichle to make a functional food and can be fortified with ingredients that

contain functional active compounds. Until now, many Indonesian researchers

have developed artificial rice products by utilizing local resources, such as

corn, sorghum, spices, tubers, and others, to obtain an artificial rice with

improved nutritional and functional characters that have some health benefits

such as antidiabetic, antioxidant, antihypertensive, and anticancer. From

mostartificial rice that have been developed, it can be seen that analog rice can

be used as a functional food for rice substitute with equal or even more (better)

nutritional value thanpaddy rice. However, further research is still needed to

improve the sensory quality of artificial rice so that it can be accepted easily by

the community.

Keywords: Artificial rice, rice analogue, functional food, food diversification

mailto:%[email protected]



PP-D-8

Critical Aspects to Produce Low-Cost Protein Molecular Weight

Marker

Leonardo Tejo Gunawan P.H. and Maria Goretti Marianti Purwanto*

Faculty of Biotechnology, University of Surabaya, Indonesia


Abstract

Proteomic studies usually begin with characterizing protein profile, like

molecular weight of protein which can be done by SDS-PAGE technique

followed by Western Blot. These methods need a standard called molecular

weight marker (MWM). MWM is used to compare the MW of protein in

standard with the protein in the samples based on their rate flow. MWM is

commercially available, however, for some researchers, the price are quite

expensive coupled with long time waiting period. In this review, the important

basic aspects of either recombinant or native MWM production will be

discussed, including the type and effect of dyes can be used for preparing

prestained MWM. Moreover, component like buffer and polyols can also affect

the quality dan stability, also other adjuvant may be needed to increase the

robustness of MWM to lower the risk of protease contamination that can

breakdown the protein inside the MWM. Understanding those critical aspects

will help researchers to produce MWM and reduce dependence on commercial

products.

Keywords: Molecular Weight Marker (MWM), protein dyes, formulation,

stability.

mailto:%[email protected]



PP-D-9

Potency healthy Moringa oleifera (Lamk) leaf flour as home-

agroindustry in rural area of Eastren East Java - Indonesia

S S Harsono1, I Syafii2, Mustaqim2, R H Setyobudi3 and J Burlakovs4

1)Departement of Agricultural Engineering, Faculty of Agricultural Technology

University of Jember, Jl. Kalimantan No. 27, Jember 68121, Indonesia 2)Farmer Jember Association, Kesilir , Jember 67121, Indonesia

3)Waste Laboratory, University Muhamadyah of Malang, Jl. Raya Tlogomas

No. 246 Malang 65114, Indonesia 4)Faculty of Health and Life Sciences, Departemen of Biology and

Environmental Science, Linnaeus University, 391 82 Kalmar/ 351 95 Växjö,

Sweden

*Corresponding author:[email protected]

Abstract

Moringa oleifera have been widely known in Indonesia, especially in rural

areas and moorlands of Eastern East Java Province of Indonesia. Moringa leaf

flour, utilizes a group of women farmers in the surrounding area to process

food products made from Moringa leaf flour, namely crackers and Moringa

sticks, so that later there will be a partnership between a a home-agroindustry

with farmer of Moringa self. Moringa leaf flour processed products is one new

products so that there is a need for a development strategy. The study was

conducted on Kesilir Agroindustry of Jember – Indonesia. The method used is

descriptive and analytical methods. The sampling method is purposive

sampling. This study aims to determined the business collaboration between

home-agroindustry made from Moringa leaf flour and farmer of Jember.

Product revenue in one production process in agro-industry made from

Moringa leaf flour, value-added products made from Moringa leaf flour, and

prospects for agro-industry development made from Moringa leaf flour as

healthy-food materials.

Keywords: Fungsional food, healthy food, Kesilir agroindustry, Moringa

crackers, Moringa sticks



PP-D-10

Quality of milk pasteurization of ginger in cold strorage

K Khotimah1, H Chairunnisa2, B Etty1, N H Nia1 and Z Vincevica-Gaile3

1)Faculty of Agricultural and Animal Science, University Muhamadiyah of

Malang, Jl. Raya Tlogomas No. 246, Malang 65114, Indonesia 2)Faculty of Animal Science, Universitas Padjadjaran, Jl.Raya Bandung

Sumedang Km. 21, Jatinangor 45363, Indonesia 3)Department of Environmental Science, University of Latvia, Raina Blvd. 19,

Riga, LV-1586, Latvia, European Union


Abstract

Utilization of natural materials, such as ginger as a flavor and preservative is

one alternative in maintaining the quality of pasteurized milk. Ginger contains

antibacterial compounds that can prevent microbial growth so as to provide

lasting effect on pasteurized milk.. The ethanol extract and chloroform ginger

appeared to inhibit the growth of bacteria Klebsiella neumonia, Salmonella

thyphimurium, Bacillus cereus, Enterococcus fecalis and Staphylococcus

aureus. The purpose of this research is to know the physicochemical quality

which includes the specific gravity, viscosity and protein content, and the

microbiology quality and the existence of Escherichia coli as one of the

requirements of quality of pasteurized milk worth of consumption. The material

used in this research is pasteurized milk with ginger flavor and kept at cold

temperature for 4 wk for 30 d. The method used in this study is a survey with

gradual observation starting before being stored, 6 d, 12 d, 18 d, 24 d, and 30 d.

Data analysis is quantitative. The results of this study indicate that the longer

the stroge showed a decrease in viscosity and protein content, but up to day

30th there was no E coli on ginger pasteurized milk kept at 4 °C.

Keywords: anti bacterial, Escherichia coli, flavor, natural material,

preservative



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About Paper Publication

Only qualified and selected papers will be published either at

Scopus Indexed International Proceeding or Scopus Indexed

International Journals (Q3 or Q4 Journal).

The participant who willing to publish their papers along with us,

please submit the full article to [email protected] before

September 3rd 2018.

The article format can be found at

http://event.ubaya.ac.id/nrls/call-for-paper/

Please sign the “Author Consent Form” which attached on the

goodie bag or request it at our secretariat desk.

The publication fee will cost IDR 1.750.000 for the Proceeding

and IDR 2.500.000 for the Journal. The payment can be done by

transfer the right nominal to BCA Account No. 5120446772

(Marianti Purwanto).


http://event.ubaya.ac.id/nrls/call-for-paper/

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