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19% 25% 7% 22% 25% i.t. i.p. i.t. (0.1 mg/kg) i.p. (0.1 mg/kg) i.p. (0.3 mg/kg) AAL-R Thy1.1 37% 31% 33% 18% 32% CD25 CD8 VEH Supplementary Figure 1. Mice were adoptively transferred with 5x10 4 Thy1.1 + GP33/CD8 + T cells. On the following day, mice were inoculated i.n. with 1x10 5 PFU of FLU-LCMV and instilled with VEH or AAL- R (0.1 or 0.3 mg/kg) either i.t. or i.p. one hpi. Six days later, lungs were processed to a single cell suspension and analyzed by flow cytometry. Percentages of Thy1.1 + CD8 + T cells and CD25 + CD8 + T cells are depicted on scattergrams. Shown are representative samples of 3-6 different specimens.

description

VEH. AAL-R. i.t. i.p. i.t. (0.1 mg/kg). i.p. (0.1 mg/kg). i.p. (0.3 mg/kg). 19%. 25%. 7%. 22%. 25%. Thy1.1. 31%. 18%. 32%. 33%. 37%. CD25. CD8. - PowerPoint PPT Presentation

Transcript of 19%

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19% 25% 7% 22% 25%

i.t. i.p. i.t. (0.1 mg/kg) i.p. (0.1 mg/kg) i.p. (0.3 mg/kg)

AAL-R

Th

y1.1

37%31% 33% 18% 32%

CD

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CD8

VEH

Supplementary Figure 1. Mice were adoptively transferred with 5x104 Thy1.1+ GP33/CD8+ T cells. On the following day, mice were inoculated i.n. with 1x105 PFU of FLU-LCMV and instilled with VEH or AAL-R (0.1 or 0.3 mg/kg) either i.t. or i.p. one hpi. Six days later, lungs were processed to a single cell suspension and analyzed by flow cytometry. Percentages of Thy1.1+CD8+ T cells and CD25+CD8+ T cells are depicted on scattergrams. Shown are representative samples of 3-6 different specimens.