19031

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UHPLC Application ID No.: 19031 LC/MS for Trypsin Digested BSA-peptide mapping on Kinetex 2.6 μm C18 150 x 2.1mm Column: Dimensions: Order No: Elution Type: Kinetex™ 2.6 μm C18 100 Å, LC Column 150 x 2.1 mm, Ea 150 x 2.1 mm ID 00F-4462-AN Gradient Eluent A: 0.1% Formic Acid in water Eluent B: 0.1% Formic Acid in Acetonitrile Gradient Step No. Time (min) Pct A Pct B Profile: 1 0 97 3 2 40 64 36 3 42 5 95 4 42.1 97 3 Flow Rate: 0.22 mL/min Col. Temp.: 25 °C Detection: Mass Spectrometer (MS) @ amu (ambient) Analyst Note: SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime. - SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782 - Holder Part No.: AJ0-9000 ANALYTES: 1 Bovine Serum Albumin (BSA) (tryptic digest) Retention Time: 1.2 min Products used in this application: Page 1 of 1 ©2015 Phenomenex Inc. All rights reserved. For more information contact your Phenomenex Representative at [email protected] Phenomenex products are available worldwide. www.phenomenex.com [email protected]

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Transcript of 19031

  • UHPLC ApplicationID No.: 19031

    LC/MS for Trypsin Digested BSA-peptide mapping on Kinetex 2.6 m C18 150 x 2.1mm

    Column:Dimensions:Order No:Elution Type:

    Kinetex 2.6 m C18 100 , LC Column 150 x 2.1 mm, Ea150 x 2.1 mm ID00F-4462-ANGradient

    Eluent A: 0.1% Formic Acid in waterEluent B: 0.1% Formic Acid in AcetonitrileGradient Step No. Time (min) Pct A Pct BProfile: 1 0 97 3

    2 40 64 363 42 5 954 42.1 97 3

    Flow Rate: 0.22 mL/minCol. Temp.: 25 CDetection: Mass Spectrometer (MS) @ amu (ambient)Analyst Note: SecurityGuard ULTRA Guard Cartridge System extends column lifetime.

    - SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782

    - Holder Part No.: AJ0-9000

    ANALYTES:1 Bovine Serum Albumin (BSA) (tryptic digest)

    Retention Time: 1.2 min

    Products used in this application:

    Page 1 of 1

    2015 Phenomenex Inc. All rights reserved. For more information contact your Phenomenex Representative at [email protected]

    Phenomenex products are available worldwide.

    www.phenomenex.com [email protected]


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