18Nanoscle Suppl Figure03011 51020 DNA : DOX (w/w) 0 100 80 60 40 Loading efficiency of DOX (%) 20 E...
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ScrG15 Apt AptC15 AptG15 Supplementary Figure 1. The morphologies of gold-incorporated DNA nanostructures. For transmission electron microscopy (TEM) imaging, chloroauric acid was added to DNA-based nanostructures for 1 h at room temperature. After removal of free chloroauric acid following reduction, the gold-loaded DNA nanostructures were visualized by TEM. For each condition, five pictures were presented. Scale bar: 25 nm. Electronic Supplementary Material (ESI) for Nanoscale. This journal is © The Royal Society of Chemistry 2018
Transcript of 18Nanoscle Suppl Figure03011 51020 DNA : DOX (w/w) 0 100 80 60 40 Loading efficiency of DOX (%) 20 E...
ScrG15
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Supplementary Figure 1. The morphologies of gold-incorporated DNAnanostructures. For transmission electron microscopy (TEM) imaging,chloroauric acid was added to DNA-based nanostructures for 1 h at roomtemperature. After removal of free chloroauric acid following reduction,the gold-loaded DNA nanostructures were visualized by TEM. For eachcondition, five pictures were presented. Scale bar: 25 nm.
Electronic Supplementary Material (ESI) for Nanoscale.This journal is © The Royal Society of Chemistry 2018
Supplementary Figure 2. NMR spectra of stemmed DNAnanostructures.The 1D 1H NMR was performed to evaluated the imino regions of the DNAnanostrurues. The DNAs were prepared at a final concentration of 0.12 mMand the NMR spectra were recorded at 288 K under 10 % D2O condition.
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Supplementary Figure 3. Loading of DOX onto stemmed DNAnanostructures.Various weight ratios of DNA:DOX were mixed, and the loadings of DOX to Apt (A),AptC15 (B), ScrG15 (C), and AptG15 (D) were analyzed by fluorescencespectroscopy and quantified (E). The results were expressed as a mean of fourseparate experiments.
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Supplementary Figure 4. Loading of MTO onto stemmed DNAnanostructures.Various weight ratios of DNA:MTO were mixed, and the loadings of MTO to Apt (A),AptC15 (B), ScrG15 (C), and AptG15 (D) were analyzed by fluorescencespectroscopy and quantified (E). The results were expressed as a mean of fourseparate experiments.
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Supplementary Figure 5. Loading of PPIX onto stemmed DNAnanostructures.Various weight ratios of DNA:PPIX were mixed, and the loadings of PPIX to Apt (A),AptC15 (B), ScrG15 (C), and AptG15 (D) were analyzed by fluorescencespectroscopy and quantified (E). The results were expressed as a mean of fourseparate experiments.
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Supplementary Figure 6. In vitro reduction of target PTK7 expression.CCRF-CEM cells were transfected with siPTK7. Seventy-two hours post transfection, PTK7 proteins on the cell surfaces were stained with fluorescein-conjugated anti-PTK7 antibody and then analyzed by flow cytometry.
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Supplementary Figure 7. Cellular uptake of various drugs delivered by AptG15.CCRF-CEM cells were seeded onto a 48-well plate at a density of 8 × 104 cells/well. Onthe next day, the cells were left untreated or treated with Apt, drug, drug/ScrG15, ordrug/AptG15. Apt/G15-loaded drugs were DOX (A), MTO (B), or PPIX (C),respectively. After 15 min of incubation, the cellular fluorescence was evaluated by flowcytometry.
Supplementary Figure 8. ROS production of PTK7-negative, -knockdown and -positive cells in the absence of light irradiation.Ramos cells (A), PTK7-knockdown CCRF-CEM cells (B) and CCRF-CEMcells (C) were left untreated or treated with Apt, MB, MB/ScrG15, or MB/AptG15. After incubation for 15 min, the fluorescence intensity of ROS indicator was observed using a fluorescence microscopy without 660-nm light irradiation. Scale bar: 100 μm.
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Supplementary Figure 9. ROS production-inducing cell killing effect.Ramos cells (A, B), PTK7-knockdown CCRF-CEM cell (C, D), and PTK7-positive CCRF-CEM cells (E, F) were left untreated or treated with Apt, MB, MB/ScrG15, or MB/AptG15. After incubation for 15 min, some cells (B,D, F) were irradiated using the 660-nm for 10 min. On the next day, cells were stained with 2 uM of calcein-AM for 10 min. The stained live cell images were obtained using fluorescence microscopy (Leica DM IL). Scale bar: 100 μm.
