mucin was detected on NK cells and monocytes in this patient cohortas compared to healthy pregnant subjects. Higher binding of MUC16to immune cells in preeclampsia was not associated with changes inserum CA125 or the number of NK cells and monocytes as comparedto healthy pregnant women.CONCLUSION: The mucin MUC16 is expressed at high levels in ovariancancer and pregnancy and carries peptide epitopes identified as thecancer biomarker CA125. MUC16 promotes metastasis and immu-nesuppression in ovarian cancer and when released from the tumors iscaptured on the surface of circulating and peritoneal NK cells, mono-cytes and B cells that express the inhibitory receptor Siglec-9. Ourresults suggest that MUC16 binding to immune cells in pregnancylikely serves as another immunomodulating mechanism. Further-more, the pattern of MUC16 binding and Siglec-9 expression on spe-cific immune subsets has the potential to serve as a novel diagnosticmarker for detection and monitoring of preeclampsia.

186 Determinants of maternal bone mineraldensity as measured during pregnancyDonna Johnson1, Christopher Robinson1, Carol Wagner2, BruceHollis3, Thomas Hulsey4, Myla Ebeling5, Judith Shary3

1Medical University of South Carolina, Obstetrics and Gynecology,Charleston, SC, 2Medical University of South Carolina, Pediatrics-Neonatology, Charleston, SC, 3Medical University of South Carolina,Pediatrics, Charleston, SC, 4Medical University of South Carolina,Pediatric Epidemiology, Charleston, SC, 5Medical University ofSouth Carolina, Pediatrics-Epidemiology, Charleston, SCOBJECTIVE: To determine the factors associated with maternal bonemineral density of the spine and hip during pregnancy.STUDY DESIGN: A prospectively enrolled cohort of healthy pregnanciesrecruited for a randomized controlled trial of Vitamin D supplemen-tation in pregnancy underwent measurement of bone mineral density(BMD) using a DEXA scan at 15-18 weeks of gestation. Multiple linearregression (MLR) was used to determine the association of maternalage, race, BMI, smoking status, parity, and 25-hydroxyvitamin D withmaternal BMD measures.RESULTS: 239 pregnant subjects underwent BMD of the spine and hip(184 Caucasian and 55 black women). Mean BMD was greater inblacks than Caucasians in both the spine (p�0.001) and hip(p�0.001). Maternal age (p�0.001), race (p�0.001), parity(p�0.04), and BMI (p�0.001) were associated with maternal spineBMD (r2�0.25). However, only maternal BMI was associated withmaternal hip BMD (p�0.001, r2�0.35). Neither maternal 25-hy-droxyvitamin D nor smoking status were associated with maternalBMD at either site.CONCLUSION: Maternal spine and hip BMD during pregnancy is asso-ciated with different maternal characteristics. Based upon these data,the maternal spine BMD is affected by multiple factors while the hipBMD is strongly associated with maternal BMI. These data provideintriguing insight into predictors of maternal BMD during pregnancyand the premenopausal state. This is the first report of maternal BMDassessed in pregnancy.

187 Do maternal inflammatory markers correlatewith both fetal and maternal adiposity?Clare O’Connor1, Nadine Farah2, Andrew Hogan3,Vicky O’Dwyer4, Mairead Kennelly5, BernardStuart5, Donal O’Shea6, Michael Turner7

1The Coombe Women and Infants University Hospital, School of Medicine &Medical Science UCD Centre For Human Reproduction, Dublin, Ireland,2Coombe Women and Infants University Hospital, UCD Centre for HumanReproduction, Dublin, Ireland, 3St Vincents University Hospital,Immunology, Dublin, Ireland, 4Coombe Women & Infants UniversityHospital, UCD Centre for Human Reproduction, Dublin, Ireland, 5CoombeWomen & Infants University Hospital, UCD Centre for HumanReproduction, Dublin, Ireland, 6St Vincents University Hospital,Endocrinology, Dublin, Ireland, 7Coombe Women and Infants UniversityHospital, UCD Centre for Human Reproduction, Dublin, IrelandOBJECTIVE: We studied whether maternal cytokines in the third tri-mester correlated with both fetal and maternal adiposity.STUDY DESIGN: In a nested cohort study, healthy women with a sin-gleton pregnancy were recruited at their convenience after a diagnos-tic Glucose Tolerance Test excluded Gestational Diabetes Mellitus(GDM). Consent was obtained. At 28 and 37 weeks gestation, mater-nal cytokines including TNF-alpha, IL-�, IL-6 and IL-8 levels wereassayed. Maternal fat mass was measured directly using advanced bio-electrical impedance analysis and fetal fat was measured using ultra-sound. Maternal Body Mass Index (BMI) had been measured in thefirst trimester and birth weight was measured after delivery.RESULTS: Of the 71 women studied, the mean age was 29.1 years andthe mean parity was 0.8. The mean BMI was 29.2 kg/m2 and 32(45%)were obese. Of the cytokines only IL-6 at both 28 and 37 weeks cor-related with maternal BMI (p�0.04; 0.005), and fat mass (p�0.005;0.010). Maternal IL-8 at 28 weeks gestation correlated with maternalweight and fat mass (p�0.032; 0.028). None of the cytokines corre-lated with fetal fat or birth weight.CONCLUSION: Previous studies on maternal obesity and inflammationhave included women with GDM. In this propective study, the cohortwas well characterised and technological advances were used to mea-sure both maternal and fetal fat. We found that maternal inflamma-tory biomarkers, particularly IL-6, correlated with maternal adipositybut not fetal adiposity. Whether IL-6 may predict maternal or fetalcomplications of maternal obesity or not remains to be seen.

188 Analyzing the involvement of heat shock protein 27in placental implantation and its effect on eukaryotictranslation initiation factor 4E expressionDana Sadeh-Mestechkin1, Shelly Tartakover Matalon2,Meir Pomeranz3, Ami Fishman4, Gali Epstein-Shochet5, Liat Drucker5, Michael Lishner6

1Meir Medical Center, Department of Obstetrics & Gynecology, Kfar-Saba,Israel, 2Meir Medical Center, Oncogenetic Laboratory, Kfar-Saba, Israel,3Meir Medical Center, Department of Obstetrics & Gynecology,Kfar Saba, Israel, 4Meir Medical Center, Obstetrics & Gynecology,Kfar Saba, Israel, 5Meir Medical Center, Oncogenetic Laboratory,Kfar Saba, Israel, 6Meir Medical Center, Internal MedicineA and Oncogenetic Laboratory, Kfar Saba, IsraelOBJECTIVE: Successful placental implantation is essential for healthyfetal growth. During implantation cytotrophoblast cells proliferate,differentiate to extravillous trophoblast cells (EVT), migrate and in-vade into the maternal uterine blood vessels. Elevated levels of heatshock protein 27 (HSP27) were found both in EVT cells during pla-cental implantation and in preeaclamptic (PET) placentae. HSP27 hasseveral important functions including protection from apoptosis,modulation of cell migration and regulation of protein degradation bythe proteasome machinery. Lately, we demonstrated an elevatedeIF4E level in differentiating EVT cells. The elevated eukaryotic trans-lation initiation factor 4E (eIF4E) factor is essential for cell prolifera-tion, migration and protection from cell death. Generally, its level isbalanced by stabilizing factors such as 4E-BP1, HSP27 and by pro-

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