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Characteristics of thePre-Breeding Parent Lines Developed in Peachgreen peach aphid, powdery mildew,brown rot, non-acid-flesh, blood-flesh, …

Develop advanced material with new traits for peach breeders¿Which new traits are targeted to INRA-Avignon?

Traits of resistance to:- green peach aphid- peach powdery mildew- Plum Pox Virus (M strain)- brown rot (Monilinia laxa)- nematodes

Traits of “fruit quality” as: - blood-flesh (DBF and bf) - high-content in 2ary metabolites

so far little attention …

Traits of the tree habit as: - weeping shape - semi-double flower

P. davidiana

P. dulcis

Malo Konare

Pamirskij 5

Weeping Flower Peach

Rubira

Develop advanced material with new traits for peach breedersCharacteristics of resistance sources ... qualitative/quantitative

BC2 from P. davidiana

Bolinha

Rm1 - - -

Rm2 - - -

- Vr1 - -

- Vr2 - -

MP.SD-3.1 PM.SD-6.1 PPV.RD-1.1 -MP.SD-5.1 PM.SD-8.1 PPV.RD-2.1

PPV.RD-7.1

- - - ongoing (WP5)

- - - ongoing (WP5)

green peach powdery plum pox brown aphid mildew virus rot

ongoing ongoing ongoing ?

Weeping Flower Peach

Rubira

BC2 from P. davidiana

Bolinha

P. davidiana

P. dulcis

Malo Konare

Pamirskij 5

Develop advanced material with new traits for peach breedersCharacteristics of resistance sources ... first by classical phenotyping

green peach powdery plum pox brown aphid mildew virus rot

TIC(Test withInoculation

on Cultivar)

TIR(Test withInoculation

on Rootstock)

GL7GL6GL5GL4GL1 GL2 GL3

Peach genetic linkage map (8 chromosomes)

GL8

DBFblood-flesh colorbf

blood-flesh color

plweeping habit

RMiaresistance to nematodes

SD-MP.3.1resistance to green peach

aphidY/y white/yellow

flesh color

Dnon-acid flesh

gglabrous skin

S*flat fruit

PPV.RD-2.1 resistance to

PPV

PPV.RD-7.1 resistance to

PPV

Develop advanced material with new traits for peach breedersCharacteristics of resistance sources ... then mapped on genome

Rm2resistance to green peach

aphid

Vr2resistance to

peach powdery mildew

SD-MP.3.1resistance to green peach

aphid

SD-PM.8.1resistance to

powdery mildew

SD-PM.6.1resistance to

powdery mildew

SDsemi-double

flower

PPV.RD-1.1 resistance to

PPV

SSR, SNP-KASP® molecular markers available for MAB for most of these traits

Develop advanced material with new traits for peach breedersCharacteristics of the peach species ... growing cycle very short

- March 2014: crosses

- June to September: harvest and statification in cold chamber (or 6 BAP)

- Winter 2014/2015 : early selection screening under greenhouse conditions

or by Marker molecular Assisted Selection …

- Springtime & Summer 2015: growing of resistant genotypes selected previously

- March 2016: new crosses with flowers coming from resistants genotypes …

according objectives, one generation all two years is easily feasible in peach

2nd generation

3rd generation

“fast breeding” in peach (3 generations / 6 years) with a rapid progress on fruit traits …

1st generation

x

P. davidiana Summergrand

sssssssSummergrand

Develop advanced material with new traits for peach breedersCharacteristics of the peach species ... growing cycle very short

example with P. davidiana and the resistance to pow. mildew

Develop advanced material with new traits for peach breedersCharacteristics of the pre-breeding parent lines …

- di-allele crosses between resistant genitors and recurrent parents as Summergrand …

100 to 200 individuals by combination stored in conservatory

-flat fruit or blood-flesh (DBF) pre-selections

bearing 1 / 2 single resist. genes (Rm1 / Vr1)

- crosses realized to associated different resistance factors to green peach aphid powdery

mildew … in the aim to improve the durability of these resistances

Thanks for your attention