CRYOPRESERVATION for BIODIVERSITY CONSERVATION

PERINBA DANISHA .J14MG11

REDLIST OF IUCN

http://www.iucnredlist.org/

BOOK:ROLES OF CRYOPRESERVATION IN CONSERVATION OF PLANT GENETIC RESOURCES

What is Cryopreservation?

• Cryopreservation is to preserve and store the viable biological samples by the use of antifreeze (cryoprotectant) solutions and cooling to very low temperature for long-term storage

CRYOPRESERVATION FREEZING

https://www.planer.com/company/news/330-cryopreservation-to-support-biodiversity-.html

STEPS INVOLVED IN CRYOPRESERVATION

SELECTION OF MATERIAL

PRE-TREATMENTS

ADDITION OF CRYOPROTECTANTS

VITRIFICATION

STEPWISE FREEZINGImpact of Procedural Steps and Cryopreservation Agents in the Cryopreservation,Tony V L.Bui et al

CONSERVATION TO SUPPORT BIODIVERSITY

• It prevents narrowing of biodiversity• It maintains food security• It prevents genetic drift of gametes, embryos• Safeguards genetic integrity of valuable strains• Offers viable alternative to maintaining active breeding

colony• Ease of transportation of genetic stock • Decrease of disease transmission • Speedy recovery of stocks following disasters

TO CONSERVE BIODIVERSITY

Cryopreservation can be used for   Conservation of plant germplasm  Vegetatively propagated species (root and tubers, ornamental, fruit

trees) Recalcitrant seed species (Howea, coconut, coffee)  Conservation of tissue with specific characteristics  Medicinal and alcohol producing cell lines Genetically transformed tissues Conservation of plant pathogens (fungi, nematodes)

Germplasm Cryopreservation

• The sperm, eggs, tissues, cultured cell lines, DNA and serum samples could be frozen and store in cryogene bank.

• Preserving the genomes of threatened and endangered species.

http://www.whatisthebiotechnology.com/blog/germplasm-conservation/

In Plants

• Variety of plant material can be used, including cells in tissue culture, pollen, seeds or parts of seeds, embryos, tissues from the early stages of development of mosses and ferns, buds, twigs,and meristematic (growing point) tissue.

http://www.whatisthebiotechnology.com/blog/germplasm-conservation/

http://www.whatisthebiotechnology.com/blog/germplasm-conservation/

In animals

• SERUM PRESERVATION• OOCYTE PRESERVATION• EMBRYOPRESERVATION• DNA BANKING • TISSUE BANKING

Semen Preservation

• Semen is generally cryopreserved with “slow cooling” methods.

• Optimal cooling rates for freezing semen are mostly found between 10 and 100 °C/min.

Oocyte Preservation

• Human oocyte cryopreservation(egg freezing) is a novel

technology in which a woman’s eggs (oocytes) are extracted, frozen and

stored.

Embryo Cryopreservation• Embryo is frozen to

extremely cold temperature, under such extreme conditions virtually all biological process cease, include the process of cell death.

• Under these specialized conditions embryos remain dormant

Use of cryopreservation and reproductive technologies for conservation of genetic resourcesSipke Joost Hiemstra et al

DNA BANKING• The DNA bank is an efficient, simple and long-

term method used in conserving genetic resource for biodiversity.

• Compared to traditional seed or field gene banks, DNA banks lessen the risk of exposing genetic information in natural surroundings.

• It only requires small sample size for storage and keeps the stable nature of DNA in cold storage.

DNA BANKING INTERNATIONAL EFFORT BY J.L. KARIHALO

http://www.biotech.wisc.edu/services/transgenicanimal/services/EmbryoCryopreservation2

DNA BANKING INTERNATIONAL EFFORT BY J.L. KARIHALO

TRUE CONSERVATION INVOLVES BIOLOGICAL MANIPULATION AND MANAGEMENT FOR A CHANGING WORLD

ANY QUESTIONS….?

• Http://www.Iucnredlist.Org/• Book:roles of cryopreservation in conservation of plant

genetic resources• Https://www.Planer.Com/company/news/330-

cryopreservation-to-support-biodiversity-.Html• Impact of procedural steps and cryopreservation agents in the

cryopreservation,tony v l.Bui et al• Http://www.Whatisthebiotechnology.Com/blog/germplasm-

conservation/• Use of cryopreservation and reproductive technologies for

conservation of genetic resourcessipke joost hiemstra et al• Dna banking international effort by j.L. Karihalo