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100 0 PM Relative FL 200 A B PM + - PM + + Control 5 Gy 5 Gy + Inhib 5 Gy Inhibitor ** ** Figure S4 Involvement of NF-kB activation in astrocytic differentiation in NSCs after 5 Gy of X-rays, A, Immunofluorescent detection of NF-kB activation (green) using anti-phosphorylated NF-kB at serine 536. Cells were fixed 1 hour after X-irradiation. The nuclei are counter stained with DAPI (Blue). B, Inhibition of NF-kB activation by 20 nM NF-kB activation inhibitor. NSCs were exposed to 5 Gy of X-rays with or without 20 nM NF-kB activation inhibitor and incubated in PM for 7 days. Astrocytic differentiation was determined by anti-GFAP antibody. The data represent the mean ± SEM. **p < 0.01. Bar indicates 5 µm.

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B. A. 200. Control. 5 Gy. **. **. Relative FL. 100. 0. 5 Gy + Inhib. PM + +. PM. PM + -. 5 Gy Inhibitor. - PowerPoint PPT Presentation

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Figure S4

Involvement of NF-kB activation in astrocytic differentiation in NSCs after 5 Gy of X-rays, A, Immunofluorescent detection of NF-kB activation (green) using anti-phosphorylated NF-kB at serine 536. Cells were fixed 1 hour after X-irradiation. The nuclei are counter stained with DAPI (Blue). B, Inhibition of NF-kB activation by 20 nM NF-kB activation inhibitor. NSCs were exposed to 5 Gy of X-rays with or without 20 nM NF-kB activation inhibitor and incubated in PM for 7 days. Astrocytic differentiation was determined by anti-GFAP antibody. The data represent the mean ± SEM. **p < 0.01. Bar indicates 5 µm.

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