1 SURVEY OF BIOCHEMISTRY Protein Function. 2 PRS In a protein, the most conformationally restricted...
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Transcript of 1 SURVEY OF BIOCHEMISTRY Protein Function. 2 PRS In a protein, the most conformationally restricted...
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SURVEY OF BIOCHEMISTRY
Protein Function
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PRS
In a protein, the most conformationally restricted amino acid is_____ and the least conformationally restricted amino acid is ________.
1. Trp, Gly2. Met, Cys3. Pro, Gly4. Ile, Ala
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PRS
In a protein, the most conformationally restricted amino acid is_____ and the least conformationally restricted amino acid is ________.
1. Trp, Gly2. Met, Cys3. Pro, Gly4. Ile, Ala
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PRS
The arrangement of the regular structural elements and the positions of atoms in the protein are considered part of the ______.
1. Primary structure2. Secondary structure3. Tertiary structure4. Quaternary structure
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PRS
The arrangement of the regular structural elements and the positions of atoms in the protein are considered part of the ______.
1. Primary structure2. Secondary structure3. Tertiary structure4. Quaternary structure
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PRS
Lysine can form a salt bridge by associating with a nearby ____ residue. 1. Pro2. Ser3. Gln4. Glu
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PRS
Lysine can form a salt bridge by associating with a nearby ____ residue. 1. Pro2. Ser3. Gln4. Glu
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PRS
Noncovalent forces that stabilize protein structure include all of the following except _______.
1. The hydrophobic effect2. Salt bridges3. Disulfide bridges4. Metal-ion coordination
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PRS
Noncovalent forces that stabilize protein structure include all of the following except _______.
1. The hydrophobic effect2. Salt bridges3. Disulfide bridges4. Metal-ion coordination
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PRS
Which of the following DNA sequences is (are) palindromic?
1. AGCT2. AAGNCTT3. AGGA4. #1 and #2
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PRS
Which of the following DNA sequences is (are) palindromic?
1. AGCT2. AAGNCTT3. AGGA4. #1 and #2
5’-AGCT-3’3’-TCGA-5’
5’-AAGNCTT-3’3’-TTCNGAA-5’
5’-AGGA-3’3’-TCCT-5’
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Protein Function: Overview
• Transport• Contraction• Protection• Catalysis • Regulation
– Gene regulation– Hormonal regulation
• Structural Support
Chapter 7 - with emphasis onMyoglobin and Hemoglobin
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Why focus on Mb and Hb?
BiologicalImportance
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Why focus on Mb and Hb?
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Why focus on Mb and Hb?
• Biological Importance
• Role in Transport of O2
– Myoglobin: O2 transport to muscles
– Hemoglobin: O2 transport from lungs
• Ligand Binding– Simple binding– Cooperativity and Allosteric Interactions
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Ligand Binding: General Concepts
• How can ligand binding be:– Described?– Measured?– Regulated?
• Distinctions between binding categories– One Protein + One Ligand– One Protein + Multiple Ligands
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Heme is what O2 binds
Heme is a porphyrin prosthetic molecule
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Myoglobin and Hemoglobin
Myoglobin Hemoglobin 1 4 1 4 8 8
Mb- O2
Hb- O2
# subunits# heme groups# Alpha Helices
18% identical residues
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Structure Changes on Binding
Fig 6-38 shows Mb “breathing”
How is O2 binding to Mbdescribed?
Show on board…
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O2 Binding to Myoglobin
Shape indicatessimple binding of
O2 to Mb
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Hb has 2 conformations
Without O2
T StateWith O2 R State
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O2 Binding to Hemoglobin
Arteries carry oxygenated blood
Veins carry deoxygenated
blood
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Upcoming…
• More on Hb and Mb on Friday
• General concepts– Muscle Contraction– Antibodies
• Exam #1 on Thursday– Boggs B6A 4-5 pm