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Life Analytical Chemistry-Molecular Imaging (MI): Optical Imaging

Gaolin Liang （梁高林） , Ph. D.Professor, Ph. D. AdvisorDeptartment of Chemistry

University of Science and Technology of China

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Optical Imaging

Fluorescence imaging

Non-Fluorescence-based optical imaging

Microscopic fluorescence imaging

Macroscopic fluorescence imaging

bioluminescence imaging

optical coherence tomography

photoacoustic microscopy

tissue spectroscopy

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Fluorescence imaging: Microscopic fluorescence imaging

History: In 1839, Rudolph Wagner visualized leukocytes rolling inblood vessels within membranous translucent tissues by using brightfieldTransillumination.

Nowadays: Several imaging approaches based on fluorescence microscopy that were established for visualizing cells in vitro have recently been adapted for in vivo imaging: multiphoton microscopy, laser-scanning confocal microscopy, fibre-optic approaches and spectrally encoded endoscopy.

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Fluorescence imaging: Macroscopic fluorescence imaging

There are two main types of imaging approach: fluorescence reflectance and tomographic fluorescence.

FMT-CT/FMT-CT fusion

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Optical Imaging

Fluorescence imaging

Non-Fluorescence-based optical imaging

Microscopic fluorescence imaging

Macroscopic fluorescence imaging

bioluminescence imaging

optical coherence tomography

photoacoustic microscopy

tissue spectroscopy

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Non-Fluorescence-based optical imaging: bioluminescence imaging

luciferase–luciferin pairs

firefly (Photinus pyralis) luciferase–luciferin

Renilla reniformis luciferase–coelenterazine

Gaussia princeps luciferase–coelenterazine

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Non-Fluorescence-based optical imaging: bioluminescence imaging

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Non-Fluorescence-based optical imaging: optical coherence tomography

Optical coherence tomography is based on light scattering and can beused to image microscopic structures in vivo (at a resolution of 2–15 μmand to a depth of 1–3 mm)

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Non-Fluorescence-based optical imaging: photoacoustic microscopy

Photoacoustic microscopy uses short laser pulses to irradiate tissueand temporarily raise its temperature (by millikelvins). Thermo-elasticexpansion then causes the emission of photoacoustic waves that canbe measured by wide-band ultrasonic transducers, offering improveddepth resolution in the 3–20 mm range

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Non-Fluorescence-based optical imaging: photoacoustic microscopy

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Non-Fluorescence-based optical imaging: photoacoustic microscopy

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Non-Fluorescence-based optical imaging: photoacoustic microscopy

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Non-Fluorescence-based optical imaging: photoacoustic microscopy

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Non-Fluorescence-based optical imaging: photoacoustic microscopy

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Non-Fluorescence-based optical imaging: tissue spectroscopy

tissue spectroscopy detects relative changes in the way in whichlight interacts with tissue and has been used extensively to improve earlydetection of gastrointestinal malignancies

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