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Introduction to Introduction to Metabolic Metabolic EngineeringEngineering

Nils Blüthgen

Frank Bruggeman

Evangelos Simeonidis

Hans V. Westerhoff

School of Chemical Engineering and Analytical Science

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LECTURE CONTENTS LECTURE CONTENTS

Aims

Module contents

Learning outcomes

Timetable

Introduction to metabolic engineering in silico

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AIMSAIMS

Familiarisation with the integration Familiarisation with the integration and regulation of microbial and regulation of microbial metabolism.metabolism.

Familiarisation with the tools used to Familiarisation with the tools used to study and manipulate metabolic study and manipulate metabolic processes in cells.processes in cells.

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Module contentsModule contents

IntroductionIntroduction In silicoIn silico experimentation experimentation Metabolic control analysis Metabolic control analysis General and Thermodynamic aspects General and Thermodynamic aspects

of metabolismof metabolism Stoicheiometric aspectsStoicheiometric aspects Metabolic flux analysisMetabolic flux analysis Signal transductionSignal transduction

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Learning OutcomesLearning Outcomes

Integrate fundamental concepts of Integrate fundamental concepts of biosciences and bioprocess engineering for biosciences and bioprocess engineering for the study of metabolism the study of metabolism (biochemistry, genetic (biochemistry, genetic engineering, molecular biology, biology, stoichiometry, engineering, molecular biology, biology, stoichiometry, thermodynamics, mass balances, calculus) thermodynamics, mass balances, calculus)

Develop an understanding of the link from Develop an understanding of the link from genome to cell behaviour/bioreactor genome to cell behaviour/bioreactor performance via metabolome performance via metabolome (regulation and (regulation and control of metabolism, signalling, bioproduct formation)control of metabolism, signalling, bioproduct formation)

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Describe how physico-chemical and biological Describe how physico-chemical and biological phenomena interact across spatio-temporal phenomena interact across spatio-temporal scales scales (cell biology, compartmentalisation of (cell biology, compartmentalisation of

metabolism,metabolism, relaxation times) relaxation times)

Develop a good appreciation of the Develop a good appreciation of the multidisciplinary aspects of biotechnology.multidisciplinary aspects of biotechnology.

Learning Outcomes Learning Outcomes cont…cont…

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Time TableTime Table

Date From To  

Folder with files Lecturer Room

Wednesday 7 February 11:00 13:00     GMS R/F2

                 

Tuesday 13 February 09:00 12:00

in silico metabolic engineering (computer practical) MEFeb13 FB

R/H1 (Sackville G11)

Wednesday 14 February 10:00 12:00metabolic control analysis

(lecture) MEFeb14 FB R/F2

Tuesday 20 February 09:00 12:00

metabolic engineering: overview (lecture) with silicon cell MEFeb20 HW

R/H1 (Sackville G11)

Wednesday 21 February 10:00 13:00

metabolic engineering: stoichiometric aspects (lecture) MEFeb21 HW R/F2

Tuesday 6 March 09:00 12:00flux analysis and flux balance

analysis MEMar6 ES/NBR/H1 (Sackville

G11)

Wednesday 7 March 10:00 13:00engineering and signal

transduction MEMar7 NB R/F2