08 Cell-Cell Interactions.ppt
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Transcript of 08 Cell-Cell Interactions.ppt
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Chapter 11Chapter 11
Cell-Cell Cell-Cell InteractionsInteractions
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Why do cells interact with one another?
How do cells interact with one another?
Cell-Cell InteractionsCell-Cell Interactions
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Importance of Cell-Cell Interactions
Interactions between… Adjacent cells Distant cells
Interactions between… Adjacent cells Distant cells
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Adjacent Cells:Connections & Communication
extracellular structures cell walls (plants) extracellular matrix (animals)
cell-cell attachments middle lamella (plants) tight junctions and
desmosomes (animals)
cell-cell gaps plasmodesmata (plants) gap junctions (animals)
Cells constantly interact with other cells
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Strong ConnectionsStrong Connections
What are these What are these bars for?bars for?
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Fiber Composites: Plant Cell WallsFiber Composites: Plant Cell Walls
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Extracellular Structures
Extracellular Matrix (animal cells)
Functions Helps to define cell shape May aid in adhesion to other cells May serve protective role
Composition Fibrous “rods” – collagen (protein) Ground substance/Matrix – gel-like polysaccharides
How do these materials get outside of the cell?
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The Cell Surface:Cytoskeleton, Plasma Membrane and Extracellular Layers
ExtracellularMatrix (ECM)
Gel-formingpolysaccharides
Integrin
Fibronectin
Collagen
PlasmaMembrane
Actin filament
Cytoskeleton
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Extracellular Matrix (ECM):Extracellular Layers in Animal Cells
The structure of a cell’s ECM correlates with that cell’s function
Epithelia Cartilage
ECM quantity and composition vary by cell type
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The ECM is directly linked to cytoskeleton via integrins and fibronectins
Holds cells in place
Helps adjacent cells adhere to one another
Extracellular Matrix (ECM):Extracellular Layers in Animal Cells
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Conditions resulting from defective ECM: Scurvy – insufficient hydroxyproline, a component of collagen
(due to Vitamin C deficiency) Photoaging – loss of collagen from dermis
Extracellular Matrix (ECM):Importance
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Cell-Cell Attachments
Cell-Cell Attachments – structures that hold cells together within multicellular organisms
Tight Junctions Desmosomes
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specialized proteins in plasma membranes of adjacent cells that line up and bind to one another
form watertight seal between cells – important in epithelial tissues; prevent passage of molecules/ions between cells
Tight Junctions:Cell-Cell Attachments in Animal Cells
Membrane proteins thatform a tight
junction
Plasma membranesof adjacent
cells
Where would you expect to find tight junctions?
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cell-cell attachments that bind cytoskeletons of adjacent cells
composed of membrane proteins that link cells (cadherins) and anchoring proteins
Provide strength in smooth muscle and epithelial tissue
Desmosomes:Cell-Cell Attachments in Animal Cells
Plasma membranesof adjacent
cells
Anchoringproteins
inside cell
Membraneproteins that
link cells(cadherins)
Intermediatefilaments
Where would you expect to find desmosomes?
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Cell-Cell Attachments
Cells of the same tissue type aggregate. How do they ‘find’ and ‘stick to’ one another?
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Cadherins and Selective Adhesion
Selective adhesion – tendency of cells of the same tissue type to adhere to one another CADHERINS
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Loss of N-cadherin in endothelial cells leads to embryonic lethality.
Luo Y , Radice G L J Cell Biol 2005;169:29-34
© 2005 Rockefeller University Press
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Question 7
Colchicine is a drug that inhibits polymerization of the subunits that form microtubules. Would colchicine be effective in stopping bacterial flagella? _____
yes no
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Test Question 9
Unicellular organisms live in a wide range of habitats, from the hot springs in Yellowstone National Park to the freezing temperatures of the Antarctic. Make a prediction about the saturation status of membrane phospholipids in organisms that live in extremely cold environments versus those that live in extremely hot environments. Organisms that live in extremely cold environments are likely to have the
same number of saturated and unsaturated phospholipids. Organisms that live in extremely cold environments are likely to have highly
unsaturated phospholipids versus those that live in extremely hot environments.
Organisms that live in extremely cold environments are likely to have phospholipids with saturated tails versus those that live in extremely hot environments.
The saturation status of membrane phospholipids does not depend on the environmental conditions.
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Adjacent Cells:Connections & Communication
extracellular structures cell walls (plants) extracellular matrix (animals)
cell-cell attachments middle lamella (plants) tight junctions and
desmosomes (animals)
cell-cell gaps plasmodesmata (plants) gap junctions (animals)
Cells constantly interact with other cells
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Cell-Cell Gaps
Cell-Cell Gaps – direct connections between the cytoplasm of adjacent cells
Enables efficient communication between cells
Enables cells within a tissue to act in concert (as an integrated whole)
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Plasmodesmata:Cell-Cell Gaps in Plant Cells
Notice: cell membranes/cytoplasm are connected (continuous)
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Gap Junctions:Cell-Cell Gaps in Animal Cells
Notice: cytoplasm is connected (continuous)
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Group Question 1
What characteristics do tight junctions bestow on tissues that use these adhesions to connect adjacent cells? They allow communication between adjacent cells. They provide strong connections to resist pulling
forces. They use the extracellular matrix to indirectly
connect adjacent cells. They form a watertight barrier between the cells.
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CELLS COMMUNICATE OVER CELLS COMMUNICATE OVER LONG DISTANCES VIA LONG DISTANCES VIA HORMONES AND SIGNAL HORMONES AND SIGNAL TRANSDUCTION PATHWAYS.TRANSDUCTION PATHWAYS.
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How do they How do they communicate?communicate?
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What do all of these have in common?What do all of these have in common?
Relay a message (signal) from outside the cell into the cell.
Way of transmitting information Way of transmitting information (signal) from outside a cell to the (signal) from outside a cell to the
inside of a cell, resulting in a inside of a cell, resulting in a response.response.
= Signal Transduction:
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Cell Signaling Pathway
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Cell Signaling Pathways (General)
Signal Sent – intercellular signal (hormone) released
1. Signal Reception – hormone binds to signal receptor
2. Signal Transduction – transmission/conversion of a signal from outside the cell into the cell.
3. Signal Amplification – signal magnified as it is “transported” through cell to its final destination.
4. Signal Response – signal reaches intracellular target(s) and triggers a cellular response (activation/deactivation)
5. Signal Deactivation – intracellular signal is turned off
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Stages of Signal Stages of Signal TransductionTransduction
(5. Signal deactivation)
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Signal Sent: Hormones
Hormones – chemical signals produced by one set of cells that affect a different set of cells Most are distributed by the bloodstream to target cells Only affect cells that have a receptor for that specific
hormone (target cells)
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Hormones
Steroid Hormones – hormones that are composed of four carbon rings (steroid structure)
Peptide (Non-Steroid) Hormones – hormones that are peptides, proteins, glycoproteins or modified amino acids
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Hormones
There are others too!
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Signal Reception
Peptide hormones bind to receptors on the cell surface.
Steroid hormones bind to receptors inside the cell.
Why?
Upon hormone binding, signal receptors change conformation (shape) resulting in a change in activity
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Signal Reception
Signal receptors… Are specific (bind specific hormones) Vary between cells (different cell types have different sets of
signal receptors) Are dynamic (numbers may increase/decrease) Can be blocked (often used as drug target);
Example: Tamoxifen
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Signal Transduction Pathways
Cell activity changes
Receptorproteinin membrane
Inside of cell
Intracellularsignal
Outside of cell
Intercellularsignal
Signal Reception
Signal Transduction• extraintracellular signal
Signal Response
Signal Amplification
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Signal Transduction Pathways
G ProteinsEnzyme-
Linked Receptors
Signal Amplification:
Second Messengers
Signal Amplification:
Phosphorylation Cascade
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Second Messengers
Second Messengers – nonprotein intracellular signaling molecules
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Signal Responses
Intracellular Targets:
DNA – activate or deactivate gene expression (making a protein using DNA “instructions”)
Pre-existing proteins – activate or deactivate enzymes, membrane channels, etc.
Result… change in cell activities!
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Signal Deactivation
Deactivation allows signal to be turned off quickly Hydrolysis of GTP by G proteins deactivates them Deactivation or sequestration of second messengers Dephosphorylation
Enables cells to respond rapidly to changing conditions
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Group Question 2
What does it mean to say that a signal is transduced? The signaling molecule enters the cell directly and
binds to a receptor inside. The physical form of the signal changes between the
outside of the cell and the inside. The signal is amplified, such that even a single
molecule evokes a large response. The signal triggers a sequence of phosphorylation
events inside the cell.
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Steroid Hormone Signaling: Example
http://web.virginia.edu/Heidi/chapter34/Images/8883n34_42.jpg
Estrogen – triggers production of proteins that promote cell proliferation (multiplication)
Estrogen – triggers production of proteins that promote cell proliferation (multiplication)
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Steroid Hormone Signaling: Example
http://web.virginia.edu/Heidi/chapter34/Images/8883n34_42.jpg
Tamoxifen binds to the estrogen receptor (prevents estrogen binding)
Tamoxifen binds to the estrogen receptor (prevents estrogen binding)
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Steroid Hormone Signaling: Example
http://web.virginia.edu/Heidi/chapter34/Images/8883n34_42.jpg
Tamoxifen binds to the estrogen receptor (prevents estrogen binding)
Inhibits cell proliferationInhibits tumor growth!
Tamoxifen binds to the estrogen receptor (prevents estrogen binding)
Inhibits cell proliferationInhibits tumor growth!