031 Identification.pf

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KMITL Sci. J. Vol.8 No.2 (Section B) July December, 28

IDENTIFICATION AND DETERMINATION METHODS OF

SYNTHETIC SWEETENER, ASPARTAME

K!e"orn N#nt#c!it$, Som%orn &utiy#n#n$#n' &r#%#rt &!ooi#n$

$De%#rtment o* &!#rm#ceutic#l Science, +#culty o* &!#rm#cy, !i#n M#i -nier"ity,

!i#n M#i, /2.

ABSTRACT

0"%#rt#me i" t!e 'eri#tie o* 'i%e%ti'e !ic! i" L1#"%#rtyl1L1%!enyl#l#nine met!yl e"ter. (1) 0"%#rt#me i"# "ynt!etic "eetener !ic! i" e%en"ie #n' it c#n i"omeri"e to be r#cem#te *orm r#%i'ly i* it i" 3e%tun%ro%erly. No1#1'#y" t!ere i" no re%ort #bout toicity o* r#cem#te *orm o* #"%#rt#me it m#y be toic li3e

r#cem#te *orm o* t!#li'omi'e. T!e #im o* t!i" "tu'y #" to "e#rc! *or i'enti*ic#tion #n' 'etermin#tionmet!o'" o* "ynt!etic "eetener, #"%#rt#me. I'enti*ic#tion met!o'" ere te"te' it! nin!y'rin "olution #n'

te"te' it! #l3#line !y'royl#mine "olution by u"in t!in l#yer c!rom#tor#m (TL). TL ere'eelo%e' it! #cetonitrile %!o"%!#te bu**er. Te"tin it! nin!y'rin "olution #" "en"itie (limit o*

'etection #" 4./ ) but #" not "%eci*ic bec#u"e t!i" met!o' #" u"e' to 'etect #mino #ci' !#in # *ree

1#mino rou%. Te"tin it! #l3#line !y'royl#mine "olution (limit o* 'etection #" 5 ) #" "%eci*ic

met!o' bec#u"e it #" u"e' to 'etect c#rbonyl *unction#l rou% #n' *orme' bron color com%le it!*erric c!lori'e. T!e 'etermin#tion met!o'" ere colorimetric met!o' (re#ction it! nin!y'rin "olution #"t!e "#me #" i'enti*ic#tion met!o') #n' !i! %re""ure li6ui' c!rom#tor#%!ic met!o' (7&L). T!e rel#tie

"t#n'#r' 'ei#tion o* colorimetric met!o' o* "im%le "u#r "#m%le" #" /.21$4.29 #n' 7&L met!o'#" 2.:1$$.49 #n' t!e %ercent recoery o* t!e"e to met!o'" ere #bout 49. 0"%#rt#me #" *oun'loer t!#n t!e l#bel #mount in m#ny "#m%le" #n' t!e %ercent rel#tie "t#n'#r' 'ei#tion" ere l#rebec#u"e #"%#rt#me #" # c!ir#l com%oun' !ic! coul' be 'e"troye' by #ir, li!t, !e#t #n' moi"ture i* it#" 3e%t un%ro%erly. T!e"e t!ree #n#lytic#l met!o'" c#n be u"e' to ine"ti#te t!e u"e o* #"%#rt#me inT!#il#n' in or'er to "timul#te Mini"try o* &ublic 7e#t! to remin' t!e u"e o* #"%#rt#me.

KEYWORDS : I'enti*ic#tion, Determin#tion, 0"%#rt#me

1. INTRODUCTION1-11

Synt!etic "eetener" ere u"e' in 'i#bete" %#tient" or to control bo'y ei!t in obe"e %er"on". T!ey ere#l"o u"e' to re'uce m#nu*#cturin co"t. T!ey #e "eetne"" but 'i' not !#e c#lorie. T!ey m#y be!#rm*ul to t!e #%%lic#nt" "uc! #" cycl#m#te !ic! i" t!e c#rcinoenic #ent.

0"%#rt#me i" t!e 'eri#tie o* 'i%e%ti'e !ic! i" L1#"%#rtyl1L1%!enyl#l#nine met!yl e"ter. It !#"been u"e' in -nite' St#te" "ince $8$. 0"%#rt#me i" met#boli;e' to be %!enyl#l#nine, #"%#rtic #ci' #n'met!#nol.


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7

,=

,75

=

N

=

,=27

7 N727

Aspartam

Met#boli"e

N75

,==

N72, =7

,=27 =

,75=7

P!"#$a$a"%" Aspart%& a&%' Mt!a"($

F%)*r 1:Met#bolic %#t!#y o* #"%#rt#me

F%)*r +: -V "%ectrum o* "t#n'#r' (1) #"%#rt#me .$9 in /9 et!#nol

55


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F%)*r :-V "%ectrum o* r#cem#te *orm o* #"%#rt#me ./9 in /9 e!t#nol

. MATERIA AND METHODMatr%a$s

1. N%"!#'r%" s($*t%("$. o* nin!y'rin #" 'i""ole' in / ml o* /9 et!#nol #n' $ ml o*l#ci#l #cetic #ci'

+. A$a$%" !#'r(/#$am%" s($*t%("

S($*t%(" A7y'royl#mine !y'roc!lori'e $2./ ere 'i""ole' in met!#nol #n' ere 'ilute'

to $ ml it! t!e "#me "olent.S($*t%(" BSo'ium !y'roi'e $2./ ere 'i""ole' in met!#nol #n' ere 'ilute' to $ ml

it! "#me "olent. Solution 0 #n' B ere mie' imme'i#tely be*ore u"e.

. Frr%& &!$(r%' s($*t%("+erric c!lori'e $./ ere 'i""ole' in /9 et!#nol #n' ere'ilute' to $ ml it! t!e "#me "olent.

0. A&t("%tr%$ p!(sp!at *22r$ olume" o* #cetonitrile %lu" olume" o* :.8 >l "olution

o* %ot#""ium 'i!y'roen %!o"%!#te #n' ere %reiou"ly #'Eu"te' to %7 5.4 it! %!o"%!oric #ci'

I"str*m"t

$. S!im#';u -V 2


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F%)*r 0:!emic#l re#ction o* nin!y'rin te"t

1.+ Tst 3%t! a$a$%" !#'r(/#$am%" s($*t%("0,5

T!in l#yer c!rom#tor#m o* "#m%le #"%#rt#me #" 'eelo%e' in t!e "#me "olent #" nin!y'rinmet!o'. TL #" 'rie', "%r#ye' it! #l3#line !y'royl#mine #n' *olloe' it! !y'roc!loric #ci' ($F$)#n' 'rie'. +erric c!lori'e "olution #" "%r#ye' into TL. &o"itie te"t #" bron "%ot. Limit o* 'etection

o* t!i" met!o' #" 5 .

5/

7

,=

=

,75 N

=

,=27

7 N727

,

,

,

=

=

=

Aspartam N%"!#'r%"

7e#t

7

,=

=

,75 N

=

7 N

,

,

,

=

=,=27

7

P%". &($(r


5/8


7

N

N7

=77N

,=

75,

=7 N727

+e

,l

Br(3" &($(r

7

,=

,75

=

N

=

,=27

7 N727

Aspartam

0l3#line !y'royl#mine"olution

7

,=

75,

=

N

N7

7 N72

=N#N7

7

($) S%r#y it! 7,L ($F$) #n' 'ry

(2) S%r#y it! *erric c!lori'e "olution

F%)*r 5: !emic#l re#ction o* #l3#line !y'royl#mine "olution te"t

+. Dtrm%"at%(" mt!('s

+.1 C($(r%mtr%& mt!('1,,0,4

Te"t it! "im%le "u#r "#m%le", $ o* "#m%le #" 'i""ole' it!


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"t#n'#r' #n' "#m%le "olution" ere inEecte' in re%lic#te". Getention time #" #bout : minute" #" "!on in*iure : #n' 4.

F%)*r 4: !rom#tor#m o* "t#n'#r' r#cem#te *orm o* #"%#rt#me 2 %%m elute' it! #cetonitrile%!o"%!#te bu**er. (retention time :.55 minute", %e#3 #re# $4:4.58$5/ m0-.)

F%)*r 6: !rom#tor#m o* #"%#rt#me in "u#r "#m%le elute' it! #cetonitrile %!o"%!#te bu**er (retention

time :.5$ minute", %e#3 #re# $


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Rs*$ts a"' D%s&*ss%("

Ta$ 1: 0""#y re"ult" o* "im%le "u#r "#m%le"

S*)ar

Sa

mp$s

7-A

Mt!(' Ca$&*$at' 7 A

Ma"

SD

7R

SD

1 + 0 5

S#m%le $ 5.8 olorimetric 5.5 2.4/ 2.55 5.25 2.$2 2.: .


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REFERENCES

$ L#u, =i1?#!, S!irr1+#i Lu3 #n' ?#i1Min !#n. S%ectro%!otometric 'etermin#tion o* #"%#rt#me in"o*t 'rin3" it! nin!y'rin #" re#ent. 0n#ly"t, $88 $$54:/14:8.

2 T!#i +oo' 0ct. 24>4.

5

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