02 Microscope. A B Carrying a Microscope Return the lowest power objective in place Wrap the cord...
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Transcript of 02 Microscope. A B Carrying a Microscope Return the lowest power objective in place Wrap the cord...
02
Microscope
A B
Carrying a Microscope
• Return the lowest power objective in place
• Wrap the cord around the base
• Return dustcover
Storing The Microscope
• Use lens paper on all glass parts of the microscope.
• Clean oil immersion lens with chemicals provided by your instructor
Cleaning the Microscope
Parts of the Microscope
• Fine adjustment knob
• Stage
• Stage clips
• Aperture
• Diaphragm
• Light
• Base
• Ocular
• Body tube
• Nosepiece
• Objective lenses (10, 40, 100x)
• Arm
• Course adjustment knob
Microscope Parts
A. OcularB. Body tubeC. HeadD. Revolving nose pieceE. ObjectiveF. ArmG. StageH. DiaphragmI. Lever to move stage clipJ. Course adjustmentK. Fine adjustmentL. Light sourceM. Base
Using the microscope
• Always observe using the LOWEST POWER objective first.
• Focus using the COARSE ADJUSTMENT KNOB to bring the object into focus. Bring the object into sharp focus by using the fine adjustment knob.
• Focus, and then move to a higher power objective, if needed.
• Use only the FINE ADJUSTMENT KNOB when using the HIGHEST (longest) POWER OBJECTIVE.
• Keep both eyes open to reduce eyestrain. • Determine total magnification of the object by
multiplying the power of the ocular (10x) the power by the power of the objective.
Preparing a slide
• Using a pipet or dropper, add a drop of water or another solvent to a clean microscope slide. Then, place the specimen in the water.
• Place the edge of a coverslip on the slide so that it touches the edge of the water.
• Slowly lower the coverslip to prevent the formation of air bubbles.
40x 100x
Oil Immersion Lens
Animal Cell
Plant Cell
Cheek Cell
Dispose Biological material
Dispose Sharps
Clean Up
Microscope Parts Quiz
Microscope Quiz
1. Which objective uses oil?2. If your ocular is 10x and your
objective is 40x what is the total magnification of your image?
3. Why do you need a cover slip and how do you avoid an air bubble?
4. Where do you dispose slides?
P. 45 lab manual
sperm
blood
smooth muscle
Simple squamous epithelial
Cheek Cell
0.9% saline
Cheek Cell
saline
1. Scrape inside cheek2. Swirl toothpick with cells in saline3. Place a small drop methylene blue on saline
Cheek Cell
1. Place coverslip2. Observe under microscope3. Draw what you see4. Clean up
Cheek Cell