0 Chromatography is a method of physically separating mixtures of gases, liquids, or dissolved...

9
1 Chromatography is a method of physically separating mixtures of gases, liquids, or dissolved substances. Chromatography can be used to identify drugs, poisons and many other substances. Separation is determined by the molecular size and/or charge

Transcript of 0 Chromatography is a method of physically separating mixtures of gases, liquids, or dissolved...

  • Chromatography is a method of physically separating mixtures of gases, liquids, or dissolved substances.Chromatography can be used to identify drugs, poisons and many other substances.Separation is determined by the molecular size and/or charge

    Kendall/Hunt Publishing Company

  • All forms of chromatography involve 2 phases The Two Phases1. stationary (absorbent) phase the material on which the separation takes place2. mobile phase - the solvent transports the sampleTypes of Chromatography - 5 of them

    Kendall/Hunt Publishing Company

  • Gas ChromatographyGas Chromatographya gas separates the componentsthey are then ionized and an electrical signal is recordedmobile phase - carrier gasstationary phase - thin film of liquid

    Kendall/Hunt Publishing Company

  • HPLCHigh Performance Liquid Chromatography (HPLC)- done at room temp. - advantageous for chemicals that are heat sensitive or volatile - example LSD- a liquid (mobile phase) is pumped through a column containing fine particles (stationary phase)

    Kendall/Hunt Publishing Company

  • TLCThin - Layer Chromatography (TLC)-A plate is coated with a granular gel - usually silica gel or aluminum oxide (stationary phase)- the substance to be separated is carried up the plate by capillary action - the substance with most affinity for the plate will rise the farthest.4. Paper chromatographySame as TLC but paperis used as stationary phase

    Kendall/Hunt Publishing Company

  • ElectrophoresisElectrophoresis - Similar to TLC but the substance is separated through a gel by electric current- Due to different size and charge substances will move across the plase at different speeds.

    Kendall/Hunt Publishing Company

  • Paper Chromatography of InkTwo samples of black ink from two different manufacturers have been characterized using paper chromatography.

    Kendall/Hunt Publishing Company

  • Retention Factor (Rf)A number that represents how far a compound travels in a particular solventIt is determined by measuring the distance the compound traveled and dividing it by the distance the solvent traveled.

    Kendall/Hunt Publishing Company

  • Solve for Rf

    Kendall/Hunt Publishing Company

    Chapter 15Kendall/HuntChapter 15Kendall/HuntChapter 15Kendall/HuntChapter 15Kendall/HuntChapter 15Kendall/HuntChapter 15Kendall/HuntChapter 15Kendall/HuntChapter 15Kendall/HuntChapter 15Kendall/Hunt


Gas Chromatography. Gas Chromatograph Gas chromatography (GC), is a common type of chromatography used in analytical chemistry for separating and analyzing.

Gas Chromatography. Gas Chromatograph Gas chromatography (GC), is a common type of chromatography used in analytical chemistry for separating and analyzing.

Liquid Chromatography (LC) - UZ Leuven · 2009-02-16 · Liquid Chromatography (LC) • technique that is useful for separating ions or molecules that are dissolved in a solvent •

Liquid Chromatography (LC) - UZ Leuven · 2009-02-16 · Liquid Chromatography (LC) • technique that is useful for separating ions or molecules that are dissolved in a solvent •

INK CHROMATOGRAPHY - Universitas Brawijaya · mixtures like ink, blood, gasoline, and lipstick. In ink chromatography, you are separating the colored pigments that make up the color

INK CHROMATOGRAPHY - Universitas Brawijaya · mixtures like ink, blood, gasoline, and lipstick. In ink chromatography, you are separating the colored pigments that make up the color

CHAPTER 11 Solutions. Paper Chromatography Separating a solution by capillary action Capillary action is the attraction of a liquid to the surface of.

CHAPTER 11 Solutions. Paper Chromatography Separating a solution by capillary action Capillary action is the attraction of a liquid to the surface of.

Chapter 3 Affected Environment - Mosaic in Manateemosaicinmanatee.com/files/2015/12/Chapter03_AffectedEnvironment_LR.pdf24 Beneficiation is the process of physically separating the

Chapter 3 Affected Environment - Mosaic in Manateemosaicinmanatee.com/files/2015/12/Chapter03_AffectedEnvironment_LR.pdf24 Beneficiation is the process of physically separating the

1 Froth Flotation – Fundamental · PDF file1 1 Froth Flotation – Fundamental Principles Froth flotation is a highly versatile method for physically separating particles based on

1 Froth Flotation – Fundamental · PDF file1 1 Froth Flotation – Fundamental Principles Froth flotation is a highly versatile method for physically separating particles based on

Separating plant pigments in Papaver somniferum using ...biotapp.weebly.com/uploads/2/5/3/1/2531617/plant_pigments.pdf · Separating plant pigments in Papaver somniferum using Chromatography

Separating plant pigments in Papaver somniferum using ...biotapp.weebly.com/uploads/2/5/3/1/2531617/plant_pigments.pdf · Separating plant pigments in Papaver somniferum using Chromatography

Paper Chromatography · Web viewProtein Chemistry Laboratory Texas A&M University Paper Chromatography: Introduction Chromatography is a technique for separating mixtures into the

Paper Chromatography · Web viewProtein Chemistry Laboratory Texas A&M University Paper Chromatography: Introduction Chromatography is a technique for separating mixtures into the

Threat and Mitigation Awareness Based Upon a Model Network ...serialsjournals.com/serialjournalmanager/pdf/1464073667.pdf · However, separating the BYOD network either physically

Threat and Mitigation Awareness Based Upon a Model Network ...serialsjournals.com/serialjournalmanager/pdf/1464073667.pdf · However, separating the BYOD network either physically

What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify.

What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify.

Paper Chromatography: Separating and Identifying Metal Ions

Paper Chromatography: Separating and Identifying Metal Ions

Gas Chromatography. 4 an analytical separations technique useful for separating volatile organic compounds 4 consists of : –Flowing mobile phase (inert.

Gas Chromatography. 4 an analytical separations technique useful for separating volatile organic compounds 4 consists of : –Flowing mobile phase (inert.

Separation Techniques Methods of Separating Mixtures Magnet Filter Decant Evaporation Centrifuge Chromatography Distillation.

Separation Techniques Methods of Separating Mixtures Magnet Filter Decant Evaporation Centrifuge Chromatography Distillation.

Chapter 22 - Gas Chromatography 22 - Gas... · Chapter 22 - Gas Chromatography Column separation (gas-liquid, gas-solid) used for separating and analyzing compounds that can be vaporized

Chapter 22 - Gas Chromatography 22 - Gas... · Chapter 22 - Gas Chromatography Column separation (gas-liquid, gas-solid) used for separating and analyzing compounds that can be vaporized

TPSScience – Science Resources for TPS Students · Web viewAs03 Separating Mixtures As04 Fractional Distilation and Paper Chromatography As05 History of the Atom As06 Structure

TPSScience – Science Resources for TPS Students · Web viewAs03 Separating Mixtures As04 Fractional Distilation and Paper Chromatography As05 History of the Atom As06 Structure

Liquid Chromatography: Injection Broadening in Ion ...€¦ · Liquid Chromatography (HILIC) is excellent in separating polar compounds which dissolve in water [5,9]. Ion-Exchange

Liquid Chromatography: Injection Broadening in Ion ...€¦ · Liquid Chromatography (HILIC) is excellent in separating polar compounds which dissolve in water [5,9]. Ion-Exchange

Separation Methods · Separating Mixtures Substances in a mixture are physically combined, so processes bases on differences in physical properties are used to separate component

Separation Methods · Separating Mixtures Substances in a mixture are physically combined, so processes bases on differences in physical properties are used to separate component

Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.

Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.

+ Separation Methods. + Separating Mixtures Substances in a mixture are physically combined, so processes bases on differences in physical properties.

+ Separation Methods. + Separating Mixtures Substances in a mixture are physically combined, so processes bases on differences in physical properties.

Experiment #2: - North Seattle Collegefacweb.northseattle.edu/aburns/C162/121NLab3bTLC.docx · Web viewOne of the most modern methods of separating mixtures in chemistry is chromatography.

Experiment #2: - North Seattle Collegefacweb.northseattle.edu/aburns/C162/121NLab3bTLC.docx · Web viewOne of the most modern methods of separating mixtures in chemistry is chromatography.