+ Tools of the Biologist. + + The Invention of the Microscope - YouTube.
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Transcript of + Tools of the Biologist. + + The Invention of the Microscope - YouTube.
![Page 1: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/1.jpg)
+
Tools of the Biologist
![Page 2: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/2.jpg)
+
![Page 3: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/3.jpg)
+
The Invention of the Microscope - YouTube
![Page 4: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/4.jpg)
+The Compound Light MicroscopeOne of the most important inventions in the field of biology because it led to the discovery of the CELL!!
In 1590 the first rudimentary microscope was developed,later perfected by Galileo and later Leeuwenhoek
![Page 5: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/5.jpg)
+Microscope Safety and Care carry with two hands (by arm and
base)
Make sure the lenses and stage are clean before using (use lens paper)
![Page 6: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/6.jpg)
+The Electron MicroscopeUses beams of electrons to magnify the
image.
Living specimens cannot be observed.
![Page 7: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/7.jpg)
+The Dissecting MicroscopeProduces a 3-D image.
Useful for observing small organisms and body structure.
![Page 8: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/8.jpg)
+Slide Preparation
Specimen must be THIN for light to pass through
Stain may be needed to make organelles more visible
Coverslip should be placed at a 45 degree angle to avoid air bubbles
Making a Wet Mount for Microscopy - 1:20
![Page 9: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/9.jpg)
+Viewing a Slide
Always begin on the lowest available power (scanning)
Center specimen in the field of view (up is down, right is left)
Focus with coarse adjustment knob
Switch to low power and repeat #2 and #3
When using high power, focus only using the FINE adjustment to prevent losing sight of your specimen or damaging the slide!
![Page 10: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/10.jpg)
+UltracentrifugeSeparates the parts of a cell according to their density by whirling them in a test tube at a high speed.
![Page 11: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/11.jpg)
+Microdissection Instruments
Can be used under the microscope for removal, addition, or transfer of cell parts.
![Page 12: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/12.jpg)
+StainingStructures in a cell can be made visible by using solutions which stain them. (Bromthymol Blue or Lugol’s iodine)
![Page 13: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/13.jpg)
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![Page 14: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/14.jpg)
+Of the following, which instrument is most commonly used to observe the external features of a grasshopper's abdomen?
1) ultracentrifuge
2) microdissection instrument
3) dissecting microscope
4) electron microscope
![Page 15: + Tools of the Biologist. + + The Invention of the Microscope - YouTube.](https://reader031.fdocuments.in/reader031/viewer/2022020219/56649f565503460f94c7a887/html5/thumbnails/15.jpg)
+A student observed a Paramecium under the low power objective of a microscope (100x) and then under high power (400x). The image of the Paramecium under low power, compared to the image of the same Paramecium under high power, would be
1) smaller and in a darker field of view
2) smaller and in a brighter field of view
3) larger and in a darker field of view
4) larger and in a brighter field of view