عبارلا ًملعلا رمتؤملا...to analysis, analysis of raw materials and finished...

المؤتمر العلم الرابع لقسم الكماء التحللة

جامعة الزقازق–كلة الصدلة

25/12/2017

[1]

رابعالمؤتمر العلم ال

قسم الكماء التحللهل

1027دسمبر 25 الاثنن

رئس المؤتمر

الحناوي ماجده.د.ا

التحلله الكماء قسم رئس

تحت رعاة

ا.د. محمد محمد بركه

عمد الكله

حنان النحاسا.د. وكل الكلة لشون الدراسات العلا

و البحوث



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التسجيل 09399 – 03:9

لإفتتاحا 093:9 - 09399

إنجازات القسم 00399 – 093:9

العروض البحثيه 0399 – 00399

. ياسمين أحمد د 0301 – 0399

د. ساره أنيس 03:9 – 0301

توزيع شهادات التقدير 03:1 – 03:9

توصيات المؤتمر 0399 – 03:1

برنامج و فعالات المؤتمر



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اللجنه المنظمة للمؤتمر

ا.د. هشام عزت عبد اللطف أستاذ الكماء التحلله

هبه محمد السد د. مدرس بقسم الكماء التحلله

المصريمنال سمر د. مدرس بقسم الكماء التحلله

. اسمن أحمد ابراهم د مدرس بقسم الكماء التحلله

التحلله الكماء بقسم مدرس د. ساره أنس

جتهم.م./ امان احمد ب مدرس مساعد بقسم الكماء التحلله

م.م./ نجلاء عبد الستار مدرس مساعد بقسم الكماء التحلله

التحللهمدرس مساعد بقسم الكماء م.م. رانا عادل سد

بقسم الكماء التحلله مدرس مساعد متاس / وستنا مخائلم.م.

ص/ ولاء محمد الالفى معد بقسم الكماء التحلله

و تقدرشكر



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[4]

The department aims to follow up the new trends in pharmaceutical

analysis techniques using advanced instrumentation and referring to

international periodicals in order to provide good services to the community

in the field of pharmaceutical industry and quality control. In addition, the

department aims to access the top position amongst faculty departments

considering teaching and research works.

The Analytical Chemistry department is devoted to provide students with

information and skills to allow them to identify chemicals and carry out

pharmaceutical analysis of drugs in formulations and biological fluids as

well as food and water analysis. In addition, the department is committed to

maintain a strong master level programs and PhD. in analytical chemistry to

improve research programs in quality control fields to enhance its role in the

community.

Vision

Mission



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The Department Intended Learning Outcomes of Courses (ILOs)

Analytical chemistry

1. Knowledge of basic chemical rules and factors affecting proprieties of

matter.

2. Study of molecular structure theories, chemical bonding,

thermochemistry and gas behavior.

3. Define the basic concepts of qualitative and quantitative analytical

chemistry.

4. Separate and differentiate between different groups of cations and

anions.

5. Differentiate between principles of different volumetric methods of

analysis.

6. Design appropriate experiments in the laboratory bearing in mind

technical, logical, safety and time limitations.

7. Apply different methodologies for determination of water, fat and oil

samples.

8. Communication skills among students and between students and the

staff.

9. Work effectively as part of a team to collect data and/or to produce

reports and presentations.

10. The ability to set realistic targets and manage time to meet targets

within deadlines.

11. Critical thinking and Problem solving.



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بقسم الكيمياء التحليلية هيئة التدريس و معاونيهم اعضاء

اضرار رئص انقطى أ.د/ ياجذج محمد انذا .1

اضرار )يرفرغ( أ.د.و/ عفاف ات انخر عثذانعى .2

اضرار )يرفرغ( أ.د.و./ياجذج محمد ادذ عاد .3

اضرار )يرفرغ( أ.د.و./ عثذالله عثذانعظى شهث .4

)يرفرغ( اضرار أ.د.و./ اء محمد اتراى صانخ .5

اضرار )يرفرغ( أ.د.و./ محمد جة انثهق .6

اضرار أ.د/ شاو عسخ عثذانهطف أتراى .7

)اعارج( اضرار أ.د/ جال دط رجة .8

اضرار )يرفرغ( أ.د.و./ ياجذج ضف انعايه .9

اضرار أ.د/ يرفد محمد دط .10

اضرار أ.د/ فاء انطذ دط .11

اضرار يطاعذ ) اجازج( أ.د.و/ داء محمد خهم .12

اضرار يطاعذ )اعارج( ./ شاو عثذ انعسس اشىأ.د.و .13

اضرار يطاعذ )اجازج( / ايح ادذ اضاعم أ.د.و .14

اضرار يطاعذ )اجازج( / يرج ضعذ انعساز أ.د.و .15

اضرار يطاعذ )اجازج( د. ضعاد ضهى ضف عثذ انذ .16

يذرش د/ ثح محمد انطذ .17

يذرش د/ يال ضر انصر .18

يذرش ./ ضارج محمد أصد .19

يذرش ./ اض أدذ اتراى محمد د .20

يذرش يطاعذ )تعثح خارجح( و.و./ محمد ثم محمد انططا .21

يذرش يطاعذ و.و./ اا ادذ تعجد .22

يذرش يطاعذ و.و./ جلاء عثذ انطرار .23

يذرش يطاعذ راا عادل ضذ و.و./ .24

)اجاز(يذرش يطاعذ و.و./ تط غرة .25

يذرش يطاعذ و.و./ أي محمد انطم .26

يذرش يطاعذ يراش / ضرا يخائمو.و. .27

يعذ ص/ لاء محمد الانف .28

الاداريين والفنيين و العمال

ضكرذر خعرج فارق دط .1

اخصائ يعم شاء جعح محمد .2

اخصائ يعم يرج محمد انصا .3

عايم عثذانقادردايذ عثاش .4

عايم تذع انطهة ضهى .5



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Courses in Analytical Chemistry Department

A-Bachelor of Pharmaceutical Sciences

General and Physical Chemistry

The aim of the course is to ensure that students acquire the knowledge of basic inorganic and physical chemistry necessary for understanding other chemistry courses, namely, qualitative inorganic analysis, quantitative analytical chemistry, organic chemistry and physical pharmacy.

Analytical Chemistry I

After completing the course, the student should gain several competencies in the areas of qualitative inorganic analytical chemistry (part 1: systematic separation of metal ions , cation that would enable him/her to work upon graduation in the different related fields of analysis (forensic chemistry, water analysis, environmental pollution,…..etc).

Analytical Chemistry II

The aim of this course is to provide the students with a qualitative inorganic analytical chemistry(part 2: anions analysis and difficulties encountered in the systematic separation of etal Ions

Analytical Chemistry III

The aim of this course is to ensure that the students have the necessary knowledge about acid – base titration, oxidation and reduction reactions, precipitmetry and complexometry and their applications.

Analytical Chemistry IV

The aim of this course is to ensure that the students have the necessary knowledge about gravimetric analysis in addition to applied analytical chemistry (water analysis and oil and fat analysis) and instrumental analysis. Sepectroscopy, potentiometry and conductometry

Quality Control and Quality Assurance

The aim of this course is to provide the students with information on Q.A. and Q.C. submitting analytical procedures, validation data, and samples to support the documentation of identity, quality, purity of drug substance and drug products.



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B- Bachelor of Pharmaceutical Sciences (Clinical Program):

PC 101 General and Physical Chemistry

The aim of the course is to ensure that students acquire the knowledge of basic inorganic and physical chemistry necessary for understanding other chemistry courses, namely, qualitative inorganic analysis, quantitative analytical chemistry, organic chemistry and physical pharmacy.

PC205 Pharmaceutical Analytical Chemistry (I)

Mixtures (suspensions, colloids and solutions), colligative properties of solutions (vapour pressure, osmotic pressure, effects on boiling and freezing points); Quantitative analytical chemistry comprises: acid base titrations and buffer solution, precipitimetry and gravimetry.

PC306 Pharmaceutical Analytical Chemistry (II)

An introduction to statistical analysis, oxidation-reduction titrations, (electrical properties of redox systems, factors affecting oxidation potential, redox titration curves). Complexometry (importance complexones stability titration curves, application, direct EDTA titrations, masking and demasking, non EDTA titrations).

PC 407 Instrumental Analysis

Spectrophotometric methods of analysis including; ultra-violet, visible and flame photometry, spectrofluorometry, atomic absorption & flame electrochemistry (potentiometry, conductimetry, polarography), chromatography.

PC 808 Pharmaceutical Analysis and Quality Control

Control and quality assurance, in-process control and validation, sampling process prior to analysis, analysis of raw materials and finished products using reference standards, pharmacopeal methods of stability and stability testing of drugs, performance and calibration of instruments used in pharmaceutical analysis, validation of analytical methods.



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Index of published researches:

1. A rapid stability indicating LC-method for determination of praziquantel in presence of its pharmacopoeial impurities

2. A High-Throughput Fluorometric Method for Quantitation of Besifloxacin

3. Liquid chromatography-tandem mass spectrometric determination ofpropofol in rat serum and hair at attogram level after derivatizationwith 3-bromomethyl-propyphenazone

4. Development of High-Throughput Method for Measurement of Vascular Nitric Oxide Generation in Microplate Reader

5. Fe3O4 nanoparticles on graphene oxide sheets for isolation and ultrasensitive amperometric detection of cancer biomarker proteins

6. Determination of Telmisartan and Estradiol Valerate using Magnetic Iron Oxide Nanoparticles Modified with Cetyltrimethylammonium Bromide followed by Spectrophtometry and HPLC

7. Isocratic HPLC method for simultaneous determination of Tramadol hydrochloride and Pantoprazole sodium synthetic mixture in pure forms and injection formulations

8. The utility of gold nanoparticles as an effective drug carrier and its antibacterial efficacy with gemifloxacin hcl

9. The Development of a new validated HPLC and spectrophotometric methods for the simultaneous determination of Daclatasvir and Sofosbuvir: antiviral Drugs



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Manuscripts

2016-2017



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[11]

Hisham Hashem1,2, Adel Ehab Ibrahim3, Magda Elhenawee1

1 Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Zagazig

University, Egypt

2 Faculty of Pharmacy, Pharmaceutical Chemistry Department, Jazan University, Saudi

Arabia

3 Egyptian International Pharmaceutical Industries Co. ‘‘EIPICo’’, Tenth of Ramadan

City, Egypt

Arabian Journal of Chemistry (2017) 10, S35–S41

1- A rapid stability indicating LC-method for determination of

praziquantel in presence of its pharmacopoeial impurities

ABSTRACT

This study reports for the first time about a stability indicating RP-HPLC method for

quantitative determination of Praziquantel (PZQ) in bulk powder and dosage form and

in presence of its pharmacopoeial impurities. The chromatographic separation was

carried out on (Caltrex AI) calixarene column, a relatively new packing material.

Chromatography was done using an isocratic binary mobile phase consisting of ACN

and 25 mM ammonium acetate (NH4Ac) in the ratio of 40:60 at flow rate of 1 mL min-1,

30 ᵒC and 210 nm wavelength for detection. The elution time of PZQ was found to be

6.15 ±0.03 min. The method was validated for system suitability, linearity, precision,

limits of detection and quantitation, specificity, stability and robustness. The robustness

study was done for small changes in temperature, flow rate, wavelength of detection

and% of CAN in mobile phase. Stability tests were done through exposure of the

analyte solution to five different stress conditions: Reflux with 1 N HCl, reflux with 1 N

NaOH, reflux with 30% H2O2, thermal degradation of powder and exposure to UV

radiation. Limits of detection and quantification were found to be 0.56 and 1.70 µg mL-1,

respectively. The recovery value of this method was 100.30% ±1.10 and the

reproducibility was within 1.31.



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[12]

Soad S Abd El-Hay1,2 and Heba M El-Sayed1

1Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig,

Egypt, 44519,

2Pharmaceutical Chemistry Department, Faculty of Pharmacy, King Abdul-Aziz

University, Saudi Arabia

Current Pharmaceutical Analysis, 2017, 13, 000-000

2- A High-Throughput Fluorometric Method for Quantitation of

Besifloxacin

ABSTRACT

Objective: The present study describes the development and validation of a simple

highthroughput method for the fluorometric determination of besifloxacin HCl in 96-

microwell plates.

Method: The proposed method is based on the reaction between besifloxacin HCl

and fluorescamine in borate buffer solution of pH 8.5 giving a highly fluorescent

derivative that was developed simultaneously and measured at 471 nm after

excitation at 353 nm. The different experimental conditions affecting the fluorescence

reaction were carefully investigated and optimized.

Results: The method showed linearity over the concentration range of 200–1000

ng/mL with low limits of detection (LOD) and quantitation (LOQ) of 8.47 ng/mL and

28.24 ng/mL, respectively. The relative standard deviation (% RSD) and relative error

(% RE) values did not exceed 3 % for precision and accuracy respectively. The

developed high-throughput method was successfully applied for the determination of

besifloxacin HCl in simulated tears and eye drops

Conclusion: The capability of the method for measuring micro-volume samples

makes it convenient for handling a very large number of samples simultaneously. In

addition, using micro-well plates can offer a safer and economic method for the

determination of besifloxacin HCl in pharmaceutical dosage forms



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[13]

Alaa Khedr1, Soad S.Abd El-Hay2, Ahmed K. Kammoun1 1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, P.O. Box 80260, Saudi ArabiabFaculty of Pharmacy, 2 Department of Analytical Chemistry, Zagazig University, Egypta Journal of Pharmaceutical and Biomedical Analysis 134 (2017) 195–202

3- Liquid chromatography-tandem mass spectrometric

determination ofpropofol in rat serum and hair at attogram level

after derivatizationwith 3-bromomethyl-propyphenazone

ABSTRACT A sensitive, selective and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) methodfor determination of propofol (PRO) in rat serum and hair has been developed. 3-Bromomethyl-propyphenazone was used as derivatization reagent forming propofol-methyl-propyphenazonecompound. The derivatization reaction was optimized and validated for maximum MS sensitivity. The MSinstrumental sensitivity reached to 10 attogram. The serum samples were extracted by using ChromabondC8columns, while hair samples extracted with methanol. The tendency of volatility of PRO was mini-mized by adding triethylamine to the extract before the use of nitrogen gas for evaporation of solvent.The limit of quantitation (LLOQ) was 0.01 pg/mL and the assay was linear to 10000 pg/mL. The intra-andinter-day precision (RSD%) ranged from 0.33 to 3.44% while the accuracy (Er%, relative error) were −6.4to 1.1%. The ionization suppression, due to reagent, was minimized by reacting the excess reagent withmethanol, and eluting to waste before MS ionization source (2–4.5 min). The method was successfullyapplied for detection and determination of PRO in rat serum and hair after 7–28 days from administrationof only one dose of propofol (10 mg/kg).



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Soad S. Abd El-Hay 1,2, and Christa L. Colyer 3 1 Faculty of Pharmacy, Pharmaceutical Chemistry Department, King Abdulaziz University, Jeddah 21589, Saudi Arabia 2 Faculty of Pharmacy, Department of Analytical Chemistry, Zagazig University, Zagazig 44519, Egypt 3 Department of Chemistry,Wake Forest University, Winston-Salem, NC 27109, USA; [email protected] Molecules 2017, 22, 127; doi:10.3390/molecules22010127

4- Development of High-Throughput Method for Measurement of

Vascular Nitric Oxide Generation in Microplate Reader

ABSTRACT Background: Despite the importance of nitric oxide (NO) in vascular physiology and pathology, a high-throughput method for the quantification of its vascular generation is lacking. Objective: By using the fluorescent probe 4-amino-5-methylamino-20,70-difluorofluorescein (DAF-FM), we have optimized a simple method for the determination of the generation of endothelial nitric oxide in a microplate format. Methods: A nitric oxide donor was used (3-morpholinosydnonimine hydrochloride, SIN-1). Different factors affecting the method were studied, such as the effects of dye concentration, different buffers, time of reaction, gain, and number of flashes. Results: Beer’s law was linear over a nanomolar range (1–10 nM) of SIN-1 with wavelengths of maximum excitation and emission at 495 and 525 nm; the limit of detection reached 0.897 nM. Under the optimized conditions, the generation of rat aortic endothelial NO was measured by incubating DAF-FM with serial concentrations (10–1000 M) of acetylcholine (ACh) for 3 min. To confirm specificity, Nw-Nitro-L-arginine methyl ester (L-NAME)—the standard inhibitor of endothelial NO synthase—was found to inhibit the ACh-stimulated generation of NO. In addition, vessels pre-exposed for 1 h to 400 M of the endothelial damaging agent methyl glyoxal showed inhibited NO generation when compared to the control stimulated by ACh. Conclusions: The capability of the method to measure micro-volume samples makes it convenient for the simultaneous handling of a very large number of samples. Additionally, it allows samples to be run simultaneously with their replicates to ensure identical experimental conditions, thus minimizing the effect of biological variability.



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[15]

Mohamed Sharafeldin1,2, Gregory W. Bishop1, Snehasis Bhakta1, Abdelhamid El-Sawya6, Steven L. Suib1,3, James F. Rusling1,3,4,5

1 Department of Chemistry (U-3060), University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, USA

2 Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zakazik, Sharkia, Egypt

3 Institute of Materials Science, University of Connecticut, 97 North Eagleville Road, Storrs, CT 06269, USA

4 Department of Surgery and Neag Cancer Center, University of Connecticut Health Center, Farmington, CT 06032, USA

5 School of Chemistry, National University of Ireland, Galway, University Road, Galway, Ireland

6 Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt Biosensors and Bioelectronics 91 (2017) 359–366

5- Fe3O4 nanoparticles on graphene oxide sheets for isolation and ultrasensitive amperometric detection of cancer biomarker

proteins

ABSTRACT Ultrasensitive mediator-free electrochemical detection for biomarker proteins was achieved at low cost using a novel composite of Fe3O4 nanoparticles loaded onto graphene oxide (GO) nano-sheets (Fe3O4@GO). This paramagnetic Fe3O4@GO composite (1 μm size range) was decorated with antibodies against prostate specific antigen (PSA) and prostate specific membrane antigen (PSMA), and then used to first capture these biomarkers and then deliver them to an 8-sensor detection chamber of a microfluidic immunoarray. Screen-printed carbon sensors coated with electrochemically reduced graphene oxide (ERGO) and a second set of antibodies selectively capture the biomarker-laden Fe3O4@GO particles, which subsequently catalyze hydrogen peroxide reduction to detect PSA and PSMA. Accuracy was confirmed by good correlation between patient serum assays and enzymelinked immuno-sorbent assays (ELISA). Excellent detection limits (LOD) of 15 fg/mL for PSA and 4.8 fg/mL for PSMA were achieved in serum. The LOD for PSA was 1000-fold better than the only previous report of PSA detection using Fe3O4. Dynamic ranges were easily tunable for concentration ranges encountered in serum samples by adjusting the Fe3O4@GO Concentration. Reagent cost was only $0.85 for a single 2-protein assay



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MM Ayad, HE Abdellatef, MM Hosny and YA Sharaf Analytical Chemistry Department, Zagazig University, Zagazig, Egypt Journal of Chemical and Pharmaceutical Research, 2017, 9(10):1-11

6- Determination of Telmisartan and Estradiol Valerate using Magnetic Iron Oxide Nanoparticles Modified with

Cetyltrimethylammonium Bromide followed by Spectrophtometry and HPLC

ABSTRACT Two novel, fast, and simple spectrophotometric and HPLC procedures were

developed for the determination of telmisartan and estradiol valerate by magnetic

solid-phase extraction (SPE) using magnetic Fe3O4 nanoparticles (MNPs)

modified by cetyltrimethylammonium bromide (CTAB) as an extractor and methanol

was used as desorption. Effects of different parameters influencing the extraction

efficiency of drugs were investigated and optimized. Spectrophotometric method

was monitored at λmax 230 and 209 nm with detection limit 0.016 and 0.087 μg

mL-1 for telmisartan and estradiol valerate, respectively. HPLC method was

developed with detection limits 1.63 and 7.02 ng mL-1 for telmisartan and estradiol

valerate, respectively. Finally, the proposed method has been effectively employed

in extraction and determination of the drugs in pure form, in pharmaceutical

formulations. Telmisartan can be extracted and determined in mixture with

hydrochlorothiazide, and also estradiol valerate can be analyzed in mixture with

norgestrel by the two cited methods.



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Magda Ayad, Mohammed El-Balkiny, Mervat Hosny*, Youstina Metias

Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt. Journal of Pharmacy Research 2017,11(3),210-219

7- Isocratic HPLC method for simultaneous determination of

Tramadol hydrochloride and Pantoprazole sodium synthetic mixture

in pure forms and injection formulations

ABSTRACT The aim of this work is to perform a validated High Performance Liquid

chromatographic (HPLC) method for simple estimation of tramadol hydrochloride

(TH) and pantoprazole sodium (PNT) synthetic mixture simultaneously in bulk form

and in I.V. pharmaceutical dosage form. The chemical stability of the mixture was

studied after dilution with different I.V. infusion solutions as 5% Dextrose, 0.9%

Sodium chloride and Ringer –Lactate solutions at 4°C, 25°C and 35°C at several

time intervals with physical examination. Chromatographic separation was carried on

reversed-phase agilent ZORBAX ODS ® C18 column (250x4.6 mm I.D., 5 μm) at

ambient temperature. Isocratic mobile phase was composed of methanol, acetonitrile

and 0.03 M phosphate buffer with 0.1% triethylamine, pH=3.5, (15:45:45, v/v) at 1.2

ml/min and detected at 210 nm. The assay showed good linearity over the

concentration range of 10-200, 2.5-60 μg/ml for TH and PNT respectively. The

analytical performance of the proposed method including linearity ranges, precision,

accuracy, detection, and quantification limits were statistically validated according to

ICH guidelines.



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Amira S. Eldin1, Shereen M. Azab1, Abdallah A. Shalaby2 and Magda El-Maamly2.

1. Pharmaceutical Chemistry Dept., National Organization for Drug Control and Research [NODCAR], 6 Abu Hazem Street, Pyramids Ave, P.O. Box 29, Giza, Egypt. 2. Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, P.C.44 519, Zagazig, Egypt. Int. J. Adv. Res. 2017, 5(8), 1649-1658

8- The utility of gold nanoparticles as an effective drug carrier and

its antibacterial efficacy with gemifloxacin Hcl

ABSTRACT A simple and extractive spectrophotometric method was developed for the

determination of Gemifloxacin HCl (GEM) in bulk drug and pharmaceutical

formulation. The method depends on the reaction between Gemifloxacin and gold

nanoparticles (AuNps). The apparent molar absorptivity and Sandell’s sensitivity were

found to be 0.1 x 104 L mol−1 cm−1 and 0.0038 μg cm-2 respectively. Beer’s law was

conformed in the concentration range of 1.5 –38.8 μg mL-1. The method is very

simple and not time consuming, therefore it can be properly applied for the evaluation

of Gemifloxacin in dosage forms and also in vitro antibacterial activities of GEM, gold

nanoparticles and drug coated gold nanoparticles against several strains of Gram

positive and Gram negative organisms. The antibacterial properties of GEM- AuNps

complex were higher than that of the pure Gemifloxacin while gold nanoparticles did

not produce any antibacterial activity against the bacterial strains. These results

recommend that gold nanoparticles could act as an effective drug carrier in drug

delivery system.



25/12/2017

[19]

Amira S. Eldin1, Shereen M. Azab1, Abdallah A. Shalaby2 and Magda El-Maamly2.

1. Pharmaceutical Chemistry Dept., National Organization for Drug Control and Research [NODCAR], 6 Abu Hazem Street, Pyramids Ave, P.O. Box 29, Giza, Egypt. 2. Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, P.C.44 519, Zagazig, Egypt. Journal of Pharmacy and Pharmacology Research 2017; 1 (1): 028-042

9- The development of a new validated HPLC and

Spectrophotometric methods for the Simultaneous determination of

Daclatasvir and Sofosbuvir: Antiviral Drugs

ABSTRACT Objective: Simple, rapid and reproducible reversed-phase high performance liquid

chromatography (RP-HPLC) and ultraviolet derivative spectrophotometric (UVDS)

methods for the simultaneous determination of daclatasvir (DAC) and sofosbuvir

(SOF) in pure and in pharmaceutical dosage forms have been developed and

validated.

Methods:The chromatographic separation of DAC and SOF was achieved using

Agilent Zorbax SB C18 (4.6 x 250 mm, 5 μm) column at temperature of 40 °C. The

mobile phase used was 9 mM dipotassium hydrogenorthophosphate buffer (pH

4±0.1): acetonitrile (60:40, v/v). The flow rate was maintained at 1 mL/min with UV

detection at 265 nm.

Results:The calculated resolution was 4.56 (> 2), which ensures complete

separation. The tailing factor was 1.13 and 1.40 (≤ 2) for DAC and SOF, respectively.

Intermediate precision value was ≤ 2% indicate acceptable ruggedness.

Conclusion:The two methods were validated according to the International

Conference on Harmonization (ICH) guidelines in terms of linearity, precision,

accuracy, selectivity, specificity, detection limit, quantification limit,

robustness and ruggedness.



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[20]

Scientific activities

2016-2017



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[21]

Challenges and Trends in Antibody-drug conjugate

Quantification and Characterization

Omnia Ismaiel CROFG NEWSLETTER, OCTOBER 2017

Do we have mature LC–MS/MS methodology for therapeutic

monoclonal antibody bioanalysis? Omnia Ismaiel Bioanalysis interview, 2017

Prostate Cancer Biomarker Detection Using a 16-Sensor Electrochemical Microfluidic Immunoarray

Abby Jones, Mohamed Sharafeldin, Min Shen, Conan Mercer, Donal Leech

Oral Presentation, The 68th Annual Meeting of the International Society of Electrochemistry International Society. Electrochemistry without Borders 27 August to 1 September, 2017, Providence, RI, USA

Automated Microcontrolled Microfluidics with Electrochemical Prostate Cancer Diagnostics

Mohamed Sharafeldin, Min Shen, James Rusling, Donal Leech Oral Presentation, The 68th Annual Meeting of the International Society of Electrochemistry International Society. Electrochemistry without Borders 27 August to 1 September, 2017, Providence, RI, USA



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[22]

( 2017 -)ماجستر إبراهم متساس مخائل وستنا ص/

( 2017 -ماجستر) سالم إبراهم محمد أحمدص/

( 2017 -)ماجستر شرف كامل شرف إناسص/

( 2017 -دكتوراه ) ابراهم أحمد اسمنم.م./

عا انرضان اضى انثادثذارخ

انرطجم أضاء نج الاشراف

ص/ إضراء يصهذ أدذ -1

أدذ

" غرق ذذهم آنح نرقذر تعط

الأدح انطرخذيح ف علاج أيراض

انشخخح

11/1/2016 أ.د عفاف أت انخر عثذ انعى

جذج محمد انذا أ.د/ يا

ص/ أيرج صلاح انذ عثذ -2

انادذ محمد

" غرق آنح نرذهم تعط يعاداخ

انكرتاخ "

11/1/2016

أ.د/ عثذ الله عثذ انعظى شهث

أ.د/ ياجذج ضف أنعايه

د./ شر يض عسب

رك يجذ محمد عرص/ -3

انعثاض

انر الأدح نثعط ذذههح دراضح"

"انذر انجاز عه ذعم 9/1/2017

عاد أدذ محمد ياجذج/ د.أ

الله فرخ تلال الله فرخ/ د.أ

دط محمد يرفد/ د.أ

انعقه محمد الله أيح/ ص -4

غاض

يعاداخ أدح نثعط ذذههح دراضح"

"انطراي يعاداخ انكرتاخ 9/1/2017

أي ادذ انطذ علاء/ د.أ

دط انطذ فاء/ د.أ

انصر ضر يال./ د

ص/ مصطفى احمد فصل -5 حلم عبد الحلم

"دراسة تحللة لبعض أدوة مضادات

السرطان"

9/1/2017

أ.د/ وفاء السد حسن عبد اللطف د. / منال سمر المصري د الحلم محمد هنداوي د./ حسن عب

فرج عل عماد إمان/ ص -6

انر الأدح تعط نرذهم آنح غرق"

انركس انعصث انجاز عه ذعم

" انذو ف نهطكر انخافع الاد

13/11/2017 اللطف عبد حسن السد وفاء/ د.أ

د الحلم محمد هنداوي د./ حسن عب

والدكتوراة رسائل الماجستر

التى تمت مناقشتها

2016-2017

المسجله رسائل الماجستر

2017- 2016



25/12/2017

[23]

أضاء نج الاشراف ذارخ انرطجم عا انرضان اضى انثادث

دالا وجه زدان عطوة -1

أدح تعط نرذهم آنح غرق"

يعاداخ انكرتاخ انعاداخ

" نهطرغا

9/1/2017

شهث انعظى عثذ عثذالله/ د.أ

دط انطذ فاء/ د.ا

انصر انذذ عثذ ضر يال/ د

عه انطذ تذرا محمد -2

انعظ

انر الأدح نثعط الان انرذهم "

انط كثار اظطراتاخ ف ذطرخذو

" انكه انععلاخ اظطراتاخ

13/11/2017 انعايه ضف ياجذ/ د.أ

انهطف عثذ دط انطذ فاء/ د.أ

ادذ عه ادذ ف -4 دذث ذقاخ ذطثك اضرثاغ"

"انثطر الاد تعط نرع13/11/2017

انخر عثذ انعى أ.د عفاف أت

أ.د/ ياجذج محمد انذا

اتراى محمد اء/ د.أ

دط محمد عاد./ د.أ

انغ عثذ محمد انطذ رشذ -3

الاد نرقذر ذذهه دراضاخ"

ف انررج عه انذر

انطرذعراخ ف انق صرذا

"انخرهف انصذن

11/12/2017 اتراى محمد اء/ د.أ

انذا انطذ محمد ياجذج/ د.أ

ضانى اتراى محمد ادذ -4

انر الادح نثعط ذذههح دراضح"

انجاز,انذر انجاز عه ذعم

" انعذ يعاداخ انرفط

11/12/2017

شهث انعظى عثذ عثذالله/ د.أ

دط انطذ فاء/ د.ا

د الحلم محمد هنداوي د./ حسن عب

عط اتراى صثر عر -5

انرذهه انطرق تعط ذطثك"

انصذن انخانػ تعط نرع

انجاز, انذر انجاز عه انؤثر

" انصاء انغذد أ انع

11/12/2017 عاد ياجذ/ د.أ

دط محمد يرفد/ د.أ

المسجله الدكتوراهرسائل

2017- 2016

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