« PROPOSITION DE STAGE ET DE THESEberret/jfb-main/Home_files...« PROPOSITION DE STAGE ET DE THESE...
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M2 15-‐Oct-‐12 Berret
« PROPOSITION DE STAGE ET DE THESE »
Laboratoire : Laboratoire Matière et Systèmes Complexes
Adresse : UMR 7057 Université Paris-Diderot/CNRS, Batiment Condorcet 10 rue Alice Domon et Léonie Duquet, F-75205 Paris Cedex 13
Responsable(s) de Stage : Jean-François Berret
Téléphone : 01 57 27 61 47 01 57 27 61 52 Email : [email protected]
N° et intitulé des écoles Doctorales de rattachement envisagées : ED 518 et ED 107
Titre du stage : “Protein corona” paradigm and nanotoxicity Résumé : In the last years, engineered inorganic nanoparticles have emerged as fundamental constituents in the development of nanotechnology. Concerns have been raised however regarding their potential risks for human health and for the environment. In in vivo assays however, the effective unit of interest is not the nanoparticles itself, but the particle and its “corona”, which implies the layer that forms around the particles, with associated proteins or biomolecules coming from the surrounding biological fluid. In this project, we will evaluate the validity of the “protein corona” paradigm by studying biological fluids that are close to real life biofluids and determine the corresponding nanoparticle-‐protein interactions. One fluid among all biological fluids is significantly relevant for human safety, the serum of the blood. The serum is the yellowish liquid that includes the proteins (except those used in blood clotting) and biological molecules such as antibodies, antigens, hormones, and any exogenous substances.
The present project aims to study the “Protein corona” in conditions that are close to those of real life and natural environments. This will be realized by: 1 -‐ A pertinent choice of particles known to impact human health on production sites and in polluted areas. These particles are nano-‐oxides responsible for inflammations, diseases and dysfunctions of cells. 2 – The effect of composition of the biofluid on particle corona will be studied, the goals being the identification and physical properties of the surrounding matrix and its influence on the nanoparticles/cell interactions and toxicity. Experiments assessing the protein corona will be light scattering, electrophoretic mobility, scanning and transmission electron microscopy. In vitro assays with protein coated particles will complete the study.
Recent References on this work M. Safi, H. Sarrouj, O. Sandre, N. Mignet and J.-‐F. Berret, Interactions between sub-‐10 nm iron and cerium oxide nanoparticles and 3T3 fibroblasts : the role of the coating and aggregation state, Nanotechnology 21 (2010) 145103 M. Safi, M. Yan, M.-‐A. Guedeau-‐Boudeville, H. Conjeaud, V. Garnier-‐Thibaud, N. Boggetto, A. Baeza-‐Squiban, Florence Niedergang, D. Averbeck and J.-‐F. Berret, Interactions between magnetic nanowires and living cells : Uptake, toxicity and degradation, ACS Nano 5 (7), 5354-‐5364 (2011) M. Safi, J. Courtois, M. Seigneuret, H. Conjeaud and J.-‐F. Berret, The effects of aggregation and protein corona on the cellular internalization of iron oxide nanoparticles, Biomaterials 32 (2011) 9353-‐9363
The protein corona that forms around nanoparticles dispersed in biofluids
plays an important role in the evaluation of the nanotoxicity.
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« PROPOSITION DE STAGE ET DE THESE »
Laboratoire : Laboratoire Matière et Systèmes Complexes
Adresse : UMR 7057 Université Paris-Diderot/CNRS, Batiment Condorcet 10 rue Alice Domon et Léonie Duquet, F-75205 Paris Cedex 13
Responsable(s) de Stage : Jean-François Berret
Téléphone : 01 57 27 61 47 01 57 27 61 52 Email : [email protected]
N° et intitulé des écoles Doctorales de rattachement envisagées : ED 518 et ED 107
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Transmission electron microscopy image of 7 nm nanoceria internalized by murine fibroblasts. The vesicles on the right-hand side are endosmomes
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« PROPOSITION DE STAGE ET/OU DE THESE »
Laboratoire : Laboratoire Matière et Systèmes Complexes
Adresse : UMR 7057 Université Paris-Diderot/CNRS, Batiment Condorcet 10 rue Alice Domon et Léonie Duquet, F-75205 Paris Cedex 13
Responsable(s) de Stage : Jean-François Berret / Gaelle Charron (Itodys, Paris 7)
Téléphone : 01 57 27 61 47 01 57 27 61 52 Email : [email protected]
N° et intitulé des écoles Doctorales de rattachement envisagées : ED 518 and ED 107: Titre du stage : Micro-rheology of cells Résumé : /0-12134# 56# ,0-#6+5-7+-#18# 8219#:7;#;-81.<:,517#18#<:,,-.=# >,# 56#?:6-;#17#,0-#:@@25+:,517#18#;-81.<:,5176# 1.# 6,.-66-6# ,1# <:+.16+1@5+# 6:<@2-6# ,1# ;-,-.<57-# ,0-5.# A56+165,4=# B0-# .0-12135+:2#@.1@-.,5-6#18#618,#+17;-76-;#<:,,-.C# !"#"#@124<-.6C# 25DE5;#+.46,:26#1.#3-26#:.-#E6E:224#<-:6E.-;#95,0#:#.0-1<-,-.#17#6:<@2-#A12E<-6#18#,0-#1.;-.#18#'#<2=#/0-12134#17#?512135+:2#1?F-+,6#56#<1.-#+0:22-73573#657+-#?512135+:2#6:<@2-6#E6E:224#@.1A5;-#,574#:<1E7,6#18#82E5;=#G5A573#+-226#81.#576,:7+-#0:A-#A12E<-6#18#,0-#1.;-.#18#17-#@5+125,-.#H'I)'"#GJ=#B1#6,E;4#,0-#.0-12135+:2#@.1@-.,5-6#18#6E+0#6:<@2-6C#.-6-:.+0-.6#E6-#-<?-;;-;#+12215;6#9016-#<1,5176C#-5,0-.#@:665A-#1.#:+,5A-C#:+,#:6#@.1?-6#81.#,0-#21+:2#-7A5.17<-7,=#
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Transmission electron microscopy and phase contrast images of magnetic nanowires. These wires are used as embedded rheometers inside living cells