λ ff 115.000 (aq. acetonitrile) Abberior STAR 635 · Best results are obtained with freshly...
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Abberior STAR 635 Abberior GmbH Hans-Adolf-Krebs-Weg 1 37077 Gö�ngen Tel. +49 551 30724–180 E-Mail: [email protected] www.abberior.com Labeling Protocols Labeling Protocols for NHS esters and male- imids as well as immunolabeling protocols can be found at www.abberior.com Labeling Protocols Abberior STAR 635 features excep�onal bright- ness and ultralow background, delivering stunning STED images. The dye works excep- �onally well with the Abberior Instruments STED microscope as well as with the Leica STED microscope. It can be excited between 630 nm and 650 nm. For STED, a deple�on wavelength between 750 nm and 775 nm is recommended. Best results are obtained with freshly prepared samples. Absorp�on Maximum, λ abs [nm] Ex�nc�on Coefficient, ε [M -1 cm -1 ] Recommended STED Wavelength, λ STED [nm] Fluorescence Life�me, τ Fluorescence Quantum Yield, η Structure Formula Molecular weight [g/mol] Solubility Polarity Content Net Charge (at pH 4.7) 635 (PBS, pH 7.4; water; aq. acetonitrile; MeOH) 110.000 (PBS, pH 7.4) 115.000 (aq. acetonitrile) 750 - 780 2.8 ns (PBS, pH 7.4) 0.88 (PBS, pH 7.4) on request C₄₉H₅₁F₃N₄O₁₁S₂ (NHS ester) 993.1 (NHS ester) 1018.1 (maleimide) 978.1 (azide) 1665.9 (phalloidin) PBS, pH 7.4; water; DMF; DMSO; aq. acetonitrile; MeOH polar (zwi�erionic) > 90% 0 Correc�on Factor, CF 260 = ε 260 /ε max Correc�on Factor, CF 280 = ε 280 /ε max 0.26 (PBS, pH 7.4; water; aq. acetonitrile) 0.38 (PBS, pH 7.4; water) 0.41 (aq. acetonitrile) Fluorescence Maximum, λ fl [nm] 655 (PBS, pH 7.4; water) 656 (aq. acetronitrile; MeOH)
Transcript of λ ff 115.000 (aq. acetonitrile) Abberior STAR 635 · Best results are obtained with freshly...
Abberior STAR 635
Abberior GmbHHans-Adolf-Krebs-Weg 137077 Gö�ngen
Tel. +49 551 30724–180E-Mail: [email protected]
www.abberior.com
Labeling Protocols
Labeling Protocols for NHS esters and male-imids as well as immunolabeling protocols can be found at www.abberior.com
Labeling Protocols
Abberior STAR 635 features excep�onal bright- ness and ultralow background, delivering stunning STED images. The dye works excep- �onally well with the Abberior Instruments STED microscope as well as with the Leica STED microscope.
It can be excited between 630 nm and 650 nm. For STED, a deple�on wavelength between 750 nm and 775 nm is recommended.
Best results are obtained with freshly prepared samples.
Absorp�on Maximum,λabs [nm]
Ex�nc�on Coefficient,ε [M-1 cm-1]
Recommended STED Wavelength, λSTED [nm]
Fluorescence Life�me, τ
Fluorescence Quantum Yield, η
Structure
Formula
Molecular weight [g/mol]
Solubility
Polarity
Content
Net Charge (at pH 4.7)
635 (PBS, pH 7.4; water; aq. acetonitrile; MeOH)
110.000 (PBS, pH 7.4) 115.000 (aq. acetonitrile)
750 - 780
2.8 ns (PBS, pH 7.4)
0.88 (PBS, pH 7.4)
on request
C₄₉H₅₁F₃N₄O₁₁S₂ (NHS ester)
993.1 (NHS ester) 1018.1 (maleimide) 978.1 (azide) 1665.9 (phalloidin)
PBS, pH 7.4; water; DMF; DMSO; aq. acetonitrile; MeOH
polar (zwi�erionic)
> 90%
0
Correc�on Factor, CF260 = ε260 /εmax
Correc�on Factor, CF280 = ε280 /εmax
0.26 (PBS, pH 7.4; water; aq. acetonitrile)
0.38 (PBS, pH 7.4; water) 0.41 (aq. acetonitrile)
Fluorescence Maximum, λfl [nm]
655 (PBS, pH 7.4; water) 656 (aq. acetronitrile; MeOH)
Abberior STAR 635
Abberior GmbHHans-Adolf-Krebs-Weg 137077 Gö�ngen
Tel. +49 551 30724–180E-Mail: [email protected]
www.abberior.com
Labeling Protocols
Labeling Protocols for NHS esters and male-imids as well as immunolabeling protocols can be found at www.abberior.com
Labeling Protocols
Abberior STAR 635 features excep�onal bright- ness and ultralow background, delivering stunning STED images. The dye works excep- �onally well with the Abberior Instruments STED microscope as well as with the Leica STED microscope.
It can be excited between 630 and 650 nm. For STED, a deple�on wavelength between 750 and 775 nm is recommended.
Best results are obtained with freshly prepared samples.
Absorp�on Maximum,λabs [nm]
Ex�nc�on Coefficient,ε [M-1 cm-1]
Recommended STED Wavelength, λSTED [nm]
Fluorescence Life�me, τ
Fluorescence Quantum Yield, η
Structure
Formula
Molecular weight
[g/mol]
Solubility
Polarity
Content
Net Charge (at pH 4.7)
635 (PBS, pH 7.4; water; aq. acetonitrile; MeOH)
110.000 (PBS, pH 7.4) 115.000 (aq. acetonitrile)
750 - 780
2.8 ns (PBS, pH 7.4)
0.88 (PBS, pH 7.4)
on request
C₄₉H₅₁F₃N₄O₁₁S₂ (NHS ester)
993.1 (NHS ester) 1018.1 (maleimide) 978.1 (azide) 1665.9 (phalloidin)
PBS, pH 7.4; water; DMF; DMSO; aq. acetonitrile; MeOH
polar (zwi�erionic)
> 90%
0
Widefield PALM
Correc�on Factor, CF260 = ε260 /εmax
Correc�on Factor, CF280 = ε280 /εmax
0.26 (PBS, pH 7.4; water; aq. acetonitrile)
0.38 (PBS, pH 7.4; water) 0.41 (aq. acetonitrile)
Confocal STED
FluorescenceMaximum, λfl [nm]
655 (PBS, pH 7.4; water) 656 (aq. acetronitrile; MeOH)
Usage and Sa�y Informa�on
Fluorescent dye for physical/chemical research purposes, spectroscopy and op�cal microscopy in biological and biomedical applica�ons.
This product is sold for research and development purposes only and must not be used for any human or animal therapeu�c, clinical diagnos�c or other medical purpose. It is not intended for food, drug, household, agricultural or cosme�c use.
The substance has not been finally and official- ly classified. To date, no health hazards have been reported nor do we know of any unreported or unpublished health hazards. However, the substance should be treated as poten�ally hazardous.
The full safety datasheet can be downloadet from www.abberior.com
Storage & Handling
Our products are shipped at room temperature. We recommend storing our products at 4 °C for short �me periods. For long term storage we recommend to store dyes, dye conjugates and an�bodies at -20 °C to -80 °C.
We strongly recommend to avoid repeated freeze thaw cycles.
Further Abberior STAR 635 Products
NHS ester, 1 mgNHS ester, 5 mgMaleimide, 1 mgMaleimide, 5 mgBio�n, 1 mgStreptavidin, 1 mgNeutravidin, 1 mgAzide, 1 mgPhalloidin, 20 µgGoat an�-mouse IgG, 500 µg Goat an�-rabbit IgG, 500 µgGoat an�-guinea-pig IgG 500 µgGoat an�-rat IgG 500 µgGoat an�-chicken 500 µg
1-0101-002-11-0105-002-71-0201-002-01-0205-002-62-0211-002-61-1301-002-61-1401-002-51-0401-002-82-0205-002-52-0205-002-52-0012-002-72-0112-002-62-0132-002-02-0102-002-9
In addi�on to the products listed, we offer a variety of custom products. If you are interested please inquire at [email protected]
Custom products
T. Müller et al. "STED Microscopy and its Applica�ons: New Insights into Cellular Processes on the Nanoscale" ChemPhysChem 13, 1986-2000 (2012).
K. Kolmakov et al. "Polar Red-Emi�ng Rhodamine Dyes with Reac�ve Groups: Synthesis, Photophysical Proper�es, and Two-Color STED Nanoscopy Applica- �ons" Chem. Eur. J., 20, 146-157 (2014).
L. Long et al. "Drosophila Syd-1, Liprin-a, and Protein Phosphatase 2A B' Subunit Wrd Func�on in a Linear Pathway to Prevent Ectopic Accumula�on of Synap�c Materials in Distal Axons" The Journal of Neuroscience, 34 (25), 8474-8487 (2014).
Literature
Recommended Products for 2-color STED
Abberior STAR 580 Abberior STAR 600
Usage and Sa�y Informa�on
Fluorescent dye for physical/chemical research purposes, spectroscopy and op�cal microscopy in biological and biomedical applica�ons.
This product is sold for research and development purposes only and must not be used for any human or animal therapeu�c, clinical diagnos�c or other medical purpose. It is not intended for food, drug, household, agricultural or cosme�c use.
The substance has not been finally and official- ly classified. To date, no health hazards have been reported nor do we know of any unreported or unpublished health hazards. However, the substance should be treated as poten�ally hazardous.
The full safety datasheet can be downloadet from www.abberior.com
Storage & Handling
Our products are shipped at room temp- erature.
Keep at 4 °C for short term storage. For long term storage we recommend a temperatur of -20 °C to -80 °C.
We strongly recommend to avoid repeated freeze-thaw cycles.
Further Abberior STAR 635 Products
NHS ester, 1 mgNHS ester, 5 mgMaleimide, 1 mgMaleimide, 5 mgBio�n, 1 mgStreptavidin, 1 mg Neutravidin, 1 mgAzide, 1 mgPhalloidin, 20 µgGoat an�-mouse IgG, 500 µg Goat an�-rabbit IgG, 500 µg Goat an�-guinea-pig IgG, 500 µg Goat an�-rat IgG, 500 µgGoat an�-chicken IgY, 500 µg
1-0101-002-11-0105-002-71-0201-002-01-0205-002-62-0211-002-61-1301-002-61-1401-002-51-0401-002-82-0205-002-52-0205-002-52-0012-002-72-0112-002-62-0132-002-02-0102-002-9
In addi�on to the products listed, we offer a variety of custom products. If you are interested please inquire at [email protected]
Custom products
T. Müller et al. "STED Microscopy and its Applica�ons: New Insights into Cellular Processes on the Nanoscale" ChemPhysChem 13, 1986-2000 (2012).
K. Kolmakov et al. "Polar Red-Emi�ng Rhodamine Dyes with Reac�ve Groups: Synthesis, Photophysical Proper�es, and Two-Color STED Nanoscopy Applica- �ons" Chem. Eur. J., 20, 146-157 (2014).
L. Long et al. "Drosophila Syd-1, Liprin-a, and Protein Phosphatase 2A B' Subunit Wrd Func�on in a Linear Pathway to Prevent Ectopic Accumula�on of Synap�c Materials in Distal Axons" The Journal of Neuroscience, 34 (25), 8474-8487 (2014).
Literature
Recommended Products for 2 Color STED
Abberior STAR 580 Abberior STAR 600
