Post on 03-Apr-2018
7/28/2019 Water Histograms 18 Dec 2003
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12000
14000
16000
18000
20000
1.00 1.07 1.14 1.21 2.04 2.11 2.18 3.01 3.08 3.15 3.22 4.05 4
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1.00 1.07 1.14 1.21 2.04 2.11 2.18 3.01 3.08 3.15 3.22 4.05
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W
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1 8 15 22 29 36 43 50 57 64 71 78 85
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Purified Water SystemsHistograms: Typical
Uses
7/28/2019 Water Histograms 18 Dec 2003
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Histogram of Softe
Sizing Assumptions 1 2 3
RO/DI skid drawoff 445.63 445.63 445.63
HVAC: Humidification, non-cGMP areas 358 358 358
HVAC: Boiler makeup 1517 1517 1517
(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)
Total Soft Water Usage liter per hour 2320.6 2320.6 2320.6
Time (Day. Hour) 1.00 1.01 1.02
Tank Volume @Start of Hour 15000 15000 15000
Make-Up Volume 2320.6 2320.6 2320.6
Minimum tank inventory: 15000.0 L
100%
User Selectable
Initial Tank Volume 15000 L
Make-Up Rate 7500 Lph
Tank Working Volume 15000 L
Total weekly makeup 504706.22
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Histogram of Softe
Sizing Assumptions
RO/DI skid drawoff
HVAC: Humidification, non-cGMP areas
HVAC: Boiler makeup
(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)
Total Soft Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 15000.0
100%
User Selectable
Initial Tank Volume 15000
Make-Up Rate 7500
Tank Working Volume 15000
Total weekly makeup 504706.22
1 2 3
445.63 445.63 445.63
358 358 358
1517 1517 1517
2320.6 2320.6 2320.6
2.00 2.01 2.02
15000 15000 15000
2320.6 2320.6 2320.6
7/28/2019 Water Histograms 18 Dec 2003
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Histogram of Softe
Sizing Assumptions
RO/DI skid drawoff
HVAC: Humidification, non-cGMP areas
HVAC: Boiler makeup
(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)
Total Soft Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 15000.0
100%
User Selectable
Initial Tank Volume 15000
Make-Up Rate 7500
Tank Working Volume 15000
Total weekly makeup 504706.22
1 2 3
445.63 445.63 445.63
358 358 358
1517 1517 1517
2320.6 2320.6 2320.6
3.00 3.01 3.02
15000 15000 15000
2320.6 2320.6 2320.6
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Histogram of Softe
Sizing Assumptions
RO/DI skid drawoff
HVAC: Humidification, non-cGMP areas
HVAC: Boiler makeup
(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)
Total Soft Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 15000.0
100%
User Selectable
Initial Tank Volume 15000
Make-Up Rate 7500
Tank Working Volume 15000
Total weekly makeup 504706.22
1 2 3
445.63 445.63 445.63
358 358 358
1517 1517 1517
2320.6 2320.6 2320.6
4.00 4.01 4.02
15000 15000 15000
2320.6 2320.6 2320.6
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Histogram of Softe
Sizing Assumptions
RO/DI skid drawoff
HVAC: Humidification, non-cGMP areas
HVAC: Boiler makeup
(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)
Total Soft Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 15000.0
100%
User Selectable
Initial Tank Volume 15000
Make-Up Rate 7500
Tank Working Volume 15000
Total weekly makeup 504706.22
1 2 3
445.63 445.63 445.63
358 358 358
1517 1517 1517
2320.6 2320.6 2320.6
5.00 5.01 5.02
15000 15000 15000
2320.6 2320.6 2320.6
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Histogram of Softe
Sizing Assumptions
RO/DI skid drawoff
HVAC: Humidification, non-cGMP areas
HVAC: Boiler makeup
(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)
Total Soft Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 15000.0
100%
User Selectable
Initial Tank Volume 15000
Make-Up Rate 7500
Tank Working Volume 15000
Total weekly makeup 504706.22
1 2 3
445.63 445.63 445.63
358 358 358
1517 1517 1517
2320.6 2320.6 2320.6
6.00 6.01 6.02
15000 15000 15000
2320.6 2320.6 2320.6
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Histogram of Softe
Sizing Assumptions
RO/DI skid drawoff
HVAC: Humidification, non-cGMP areas
HVAC: Boiler makeup
(NOTE: RO/DI skid drawoff feeds USP PW tank. USP PW tank feeds WFI still,
clean steam generator, and CIP skids. Demands are not simultaneous.)
Total Soft Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 15000.0
100%
User Selectable
Initial Tank Volume 15000
Make-Up Rate 7500
Tank Working Volume 15000
Total weekly makeup 504706.22
1 2 3
445.63 445.63 445.63
358 358 358
1517 1517 1517
2320.6 2320.6 2320.6
7.00 7.01 7.02
15000 15000 15000
2320.6 2320.6 2320.6
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1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
SystemMak
eup,LPH
Time (Day. Hour)
Soft Water Tank Volume and Makeup
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Histogram
System Ma
Sizing Assumptions 1 2 3 4 5 6
WFI feed 0 0 0 0 0 1413
Clean steam generation 445.625 445.625 445.625 445.625 534.175 445.625 4
CIP skid, suite 1 (all except final rinse) 2149 1153
Total USP Purified Water Usage liter per hour 445.625 445.625 445.625 445.625 534.175 3011.63 1
Time (Day. Hour) 1.00 1.01 1.02 1.03 1.04 1.05 Tank Volume @Start of Hour 3000 3000 3000 3000 3000 3000 2
Make-Up Volume 445.625 445.625 445.625 445.625 534.175 3000 1
Minimum tank inventory: 1534.0 L
51%
Average
User Selectable Maximum
Initial Tank Volume 3000 L Maximum
Make-Up Rate 3000 Lph Total Us
Tank Working Volume 3000 L
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Histogram
System Mak
Sizing Assumptions
WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse) 2149
Total USP Purified Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 1534.0
51%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 3000
Tank Working Volume 3000
1 2 3 4 5 6
0 0 0 0 0 0
445.625 445.625 445.625 445.625 445.625 445.625 52
445.625 445.625 445.625 445.625 445.625 445.625 52
2.00 2.01 2.02 2.03 2.04 2.05 2
3000 3000 3000 3000 3000 3000 3
445.625 445.625 445.625 445.625 445.625 445.625 52
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Histogram
System Mak
Sizing Assumptions
WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse) 2149
Total USP Purified Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 1534.0
51%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 3000
Tank Working Volume 3000
1 2 3 4 5 6
0 0 0 0 0 0
445.625 445.625 445.625 445.625 445.625 736.575 52
445.625 445.625 445.625 445.625 445.625 736.575 52
3.00 3.01 3.02 3.03 3.04 3.05 3
3000 3000 3000 3000 3000 3000 3
445.625 445.625 445.625 445.625 445.625 736.575 52
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Histogram
System Mak
Sizing Assumptions
WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse) 2149
Total USP Purified Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 1534.0
51%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 3000
Tank Working Volume 3000
1 2 3 4 5 6
0 0 0 0 0 0 4
445.625 445.625 445.625 445.625 445.625 445.625 44
445.625 445.625 445.625 445.625 445.625 445.625 84
4.00 4.01 4.02 4.03 4.04 4.05 4
3000 3000 3000 3000 3000 3000 3
445.625 445.625 445.625 445.625 445.625 445.625 84
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Histogram
System Mak
Sizing Assumptions
WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse) 2149
Total USP Purified Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 1534.0
51%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 3000
Tank Working Volume 3000
1 2 3 4 5 6
0 0 0 0 0 0
445.625 445.625 445.625 445.625 445.625 445.625 44
445.625 445.625 445.625 445.625 445.625 445.625 44
5.00 5.01 5.02 5.03 5.04 5.05 5
3000 3000 3000 3000 3000 3000 3
445.625 445.625 445.625 445.625 445.625 445.625 44
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Histogram
System Mak
Sizing Assumptions
WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse) 2149
Total USP Purified Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 1534.0
51%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 3000
Tank Working Volume 3000
1 2 3 4 5 6
0 0 0 0 0 0
445.625 445.625 445.625 445.625 445.625 445.625 445
445.625 445.625 445.625 445.625 445.625 445.625 445
6.00 6.01 6.02 6.03 6.04 6.05 6
3000 3000 3000 3000 3000 3000 30
445.625 445.625 445.625 445.625 445.625 445.625 445
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Histogram
System Mak
Sizing Assumptions
WFI feed
Clean steam generation
CIP skid, suite 1 (all except final rinse) 2149
Total USP Purified Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume
Minimum tank inventory: 1534.0
51%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 3000
Tank Working Volume 3000
1 2 3 4 5 6
0 0 0 0 0 0
445.625 445.625 445.625 445.625 445.625 445.625 445
445.625 445.625 445.625 445.625 445.625 445.625 445
7.00 7.01 7.02 7.03 7.04 7.05 7
3000 3000 3000 3000 3000 3000 30
445.625 445.625 445.625 445.625 445.625 445.625 445
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1500
3000
4500
6000
7500
1.
00
1.
03
1.
06
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1.
15
1.
18
1.
21
2.
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2.
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2.
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2.
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2.
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2.
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2.
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2.
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3.
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3.
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4.
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4.
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4.
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5.
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5.
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5
1 8
SystemMakeup,LPH
Time (Day. Hour)
USP PW Tank Volume and Makeup
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Sizing Assumptions
1500 L Mammalian Media Prep
1500 L Mammalian Buffer Prep
Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00 Use points
1413.00 (max. consumption)
1780.00 Mam Suite 1, Cell Culture 25 lpm max per drop
1166.00 Mam Suite 1, V+ Purification 25 lpm max per drop
0.00 Mam Suite 1, V- Purification 25 lpm max per drop
1413.00 Suite 1 Media Preparation 25 lpm max per drop
400.00 Suite 1 Buffer Preparation 25 lpm max per drop
#REF! Suite 1 Washer
#REF!
150.00 Utility area: COP 959 l per run
500.00 Fill / finish: Dirty Parts Washer 150 l per run
500.00 Fill / finish: Vial Washer 500 l per run
706.00 Fill / finish: Clean Parts Washer 500 l per run
200.00 Fill / finish: CIP (WFI Rinse Only) 706 l per run
Fill / finish: Lyophilizer Wash-Out 200 l per run
0.00
Total WFI Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume 463.0 (time-averaged)
Minimum tank inventory: 1250.4
42%
User Selectable
Initial Tank Volume 3000Make-Up Rate 1650
Tank Working Volume 3000
1500L Mammalian Purification Process Train #1 Starts on Monday
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Sizing Assumptions
1500 L Mammalian Media Prep
1500 L Mammalian Buffer Prep
Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00 Use points
1413.00 (max. consumption)
1780.00 Mam Suite 1, Cell Culture 25 lpm max per drop
1166.00 Mam Suite 1, V+ Purification 25 lpm max per drop
0.00 Mam Suite 1, V- Purification 25 lpm max per drop
1413.00 Suite 1 Media Preparation 25 lpm max per drop
400.00 Suite 1 Buffer Preparation 25 lpm max per drop
#REF! Suite 1 Washer
#REF!
150.00 Utility area: COP 959 l per run
500.00 Fill / finish: Dirty Parts Washer 150 l per run
500.00 Fill / finish: Vial Washer 500 l per run
706.00 Fill / finish: Clean Parts Washer 500 l per run
200.00 Fill / finish: CIP (WFI Rinse Only) 706 l per run
Fill / finish: Lyophilizer Wash-Out 200 l per run0.00
Total WFI Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume 463.0 (time-averaged)
Minimum tank inventory: 1250.4
42%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 1650Tank Working Volume 3000
1500L Mammalian Purification Process Train #1 Starts on Monday
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Sizing Assumptions
1500 L Mammalian Media Prep
1500 L Mammalian Buffer Prep
Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00 Use points1413.00 (max. consumption)
1780.00 Mam Suite 1, Cell Culture 25 lpm max per drop
1166.00 Mam Suite 1, V+ Purification 25 lpm max per drop
0.00 Mam Suite 1, V- Purification 25 lpm max per drop
1413.00 Suite 1 Media Preparation 25 lpm max per drop
400.00 Suite 1 Buffer Preparation 25 lpm max per drop
#REF! Suite 1 Washer
#REF!
150.00 Utility area: COP 959 l per run
500.00 Fill / finish: Dirty Parts Washer 150 l per run
500.00 Fill / finish: Vial Washer 500 l per run
706.00 Fill / finish: Clean Parts Washer 500 l per run
200.00 Fill / finish: CIP (WFI Rinse Only) 706 l per run
Fill / finish: Lyophilizer Wash-Out 200 l per run
0.00
Total WFI Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume 463.0 (time-averaged)
Minimum tank inventory: 1250.4
42%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 1650
Tank Working Volume 3000
1500L Mammalian Purification Process Train #1 Starts on Monday
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Sizing Assumptions
1500 L Mammalian Media Prep
1500 L Mammalian Buffer Prep
Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00 Use points
1413.00 (max. consumption)
1780.00 Mam Suite 1, Cell Culture 25 lpm max per drop
1166.00 Mam Suite 1, V+ Purification 25 lpm max per drop
0.00 Mam Suite 1, V- Purification 25 lpm max per drop
1413.00 Suite 1 Media Preparation 25 lpm max per drop
400.00 Suite 1 Buffer Preparation 25 lpm max per drop
#REF! Suite 1 Washer
#REF!
150.00 Utility area: COP 959 l per run
500.00 Fill / finish: Dirty Parts Washer 150 l per run
500.00 Fill / finish: Vial Washer 500 l per run
706.00 Fill / finish: Clean Parts Washer 500 l per run
200.00 Fill / finish: CIP (WFI Rinse Only) 706 l per run
Fill / finish: Lyophilizer Wash-Out 200 l per run
0.00
Total WFI Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume 463.0 (time-averaged)
Minimum tank inventory: 1250.4
42%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 1650
Tank Working Volume 3000
1500L Mammalian Purification Process Train #1 Starts on Monday
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Sizing Assumptions
1500 L Mammalian Media Prep
1500 L Mammalian Buffer Prep
Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00 Use points
1413.00 (max. consumption)
1780.00 Mam Suite 1, Cell Culture 25 lpm max per drop
1166.00 Mam Suite 1, V+ Purification 25 lpm max per drop
0.00 Mam Suite 1, V- Purification 25 lpm max per drop
1413.00 Suite 1 Media Preparation 25 lpm max per drop
400.00 Suite 1 Buffer Preparation 25 lpm max per drop
#REF! Suite 1 Washer
#REF!
150.00 Utility area: COP 959 l per run
500.00 Fill / finish: Dirty Parts Washer 150 l per run
500.00 Fill / finish: Vial Washer 500 l per run
706.00 Fill / finish: Clean Parts Washer 500 l per run
200.00 Fill / finish: CIP (WFI Rinse Only) 706 l per run
Fill / finish: Lyophilizer Wash-Out 200 l per run0.00
Total WFI Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume 463.0 (time-averaged)
Minimum tank inventory: 1250.4
42%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 1650Tank Working Volume 3000
1500L Mammalian Purification Process Train #1 Starts on Monday
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H
Sizing Assumptions
1500 L Mammalian Media Prep
1500 L Mammalian Buffer Prep
Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00 Use points
1413.00 (max. consumption)
1780.00 Mam Suite 1, Cell Culture 25 lpm max per drop1166.00 Mam Suite 1, V+ Purification 25 lpm max per drop
0.00 Mam Suite 1, V- Purification 25 lpm max per drop
1413.00 Suite 1 Media Preparation 25 lpm max per drop
400.00 Suite 1 Buffer Preparation 25 lpm max per drop
#REF! Suite 1 Washer
#REF!
150.00 Utility area: COP 959 l per run
500.00 Fill / finish: Dirty Parts Washer 150 l per run
500.00 Fill / finish: Vial Washer 500 l per run
706.00 Fill / finish: Clean Parts Washer 500 l per run
200.00 Fill / finish: CIP (WFI Rinse Only) 706 l per run
Fill / finish: Lyophilizer Wash-Out 200 l per run
0.00
Total WFI Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume 463.0 (time-averaged)
Minimum tank inventory: 1250.4
42%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 1650
Tank Working Volume 3000
1500L Mammalian Purification Process Train #1 Starts on Monday
1
0
6.00
3000
0
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H
Sizing Assumptions
1500 L Mammalian Media Prep
1500 L Mammalian Buffer Prep
Suite 1 Washer
Fill/finish: Dirty Parts Washer
Fill / finish: Vial Washer
Fill / finish: Clean Parts Washer
Fill / finish: CIP
Fill / finish: Lyophilizer Wash-Out
1780.00 Use points
1413.00 (max. consumption)
1780.00 Mam Suite 1, Cell Culture 25 lpm max per drop
1166.00 Mam Suite 1, V+ Purification 25 lpm max per drop
0.00 Mam Suite 1, V- Purification 25 lpm max per drop
1413.00 Suite 1 Media Preparation 25 lpm max per drop
400.00 Suite 1 Buffer Preparation 25 lpm max per drop
#REF! Suite 1 Washer
#REF!
150.00 Utility area: COP 959 l per run
500.00 Fill / finish: Dirty Parts Washer 150 l per run
500.00 Fill / finish: Vial Washer 500 l per run
706.00 Fill / finish: Clean Parts Washer 500 l per run
200.00 Fill / finish: CIP (WFI Rinse Only) 706 l per run
Fill / finish: Lyophilizer Wash-Out 200 l per run
0.00
Total WFI Water Usage liter per hour
Time (Day. Hour)
Tank Volume @Start of Hour
Make-Up Volume 463.0 (time-averaged)
Minimum tank inventory: 1250.4
42%
User Selectable
Initial Tank Volume 3000
Make-Up Rate 1650
Tank Working Volume 3000
1500L Mammalian Purification Process Train #1 Starts on Monday
1
0
7.00
3000 3
0
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0
150
300
450
600
750
900
1050
1200
1350
1500
1650
1800
SystemMakeu
p,LPH
Time (Day. Hour)
Water for Injection System:System Make-Up and Tank Volume
Tank Volume WFI Makeup
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Sizing Assumptions 1 2
Suite 1 Media Prep
Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
Rooms (max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture SIP: 0.0803 kg/liter M1-P1 Mam Suite 1, V+ Purification SIP: 0.0803 kg/liter
M1-P2 Mam Suite 1, V- Purification SIP: 0.0803 kg/liter
Mam Suite 1 Buffer Preparation SIP: 0.0803 kg/liter
FF-Auto Filling Suite Autoclave 230 kg/load
End user load excluding HVAC 0 0
AHU (humidification) 352.27 352.27
Line Loss 10% 35.227 35.227
Total Pure Steam Usage kg/hr 387.5 387.5
USP Water Supply, LPH 782.285775 445.63 445.63
Time (Day. Hour) 1.00 1.01
Time-averaged production 407.5
Maximum Pure Steam Load 680 kg/hr
Line loss, maximum 68.0
Blowdown 102.0
Safety factor 20%
Pure steam generator 1100 kg/hr
Pure Steam Pressure 2.4 bar
Blowdown percentage 15%
Minimum load 35%
1500L Mammalian Purification Process Suite 1 Starts on Monday
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Sizing Assumptions
Suite 1 Media Prep
Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
Rooms (max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture SIP: 0.0803 kg/liter
M1-P1 Mam Suite 1, V+ Purification SIP: 0.0803 kg/liter M1-P2 Mam Suite 1, V- Purification SIP: 0.0803 kg/liter
Mam Suite 1 Buffer Preparation SIP: 0.0803 kg/liter
FF-Auto Filling Suite Autoclave 230 kg/load
End user load excluding HVAC
AHU (humidification)
Line Loss 10%
Total Pure Steam Usage kg/hr
USP Water Supply, LPH 782.285775
Time (Day. Hour)
Time-averaged production 407.5
Maximum Pure Steam Load 680
Line loss, maximum 68.0
Blowdown 102.0
Safety factor 20%
Pure steam generator 1100
Pure Steam Pressure 2.4
Blowdown percentage 15%
Minimum load 35%
1500L Mammalian Purification Process Suite 1 Starts on Monday
1 2
0 0
352.27 352.27 3
35.227 35.227 3
387.5 387.5 3
445.63 445.63 4
2.00 2.01
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Sizing Assumptions
Suite 1 Media Prep
Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
Rooms (max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture SIP: 0.0803 kg/liter
M1-P1 Mam Suite 1, V+ Purification SIP: 0.0803 kg/liter M1-P2 Mam Suite 1, V- Purification SIP: 0.0803 kg/liter
Mam Suite 1 Buffer Preparation SIP: 0.0803 kg/liter
FF-Auto Filling Suite Autoclave 230 kg/load
End user load excluding HVAC
AHU (humidification)
Line Loss 10%
Total Pure Steam Usage kg/hr
USP Water Supply, LPH 782.285775
Time (Day. Hour)
Time-averaged production 407.5
Maximum Pure Steam Load 680
Line loss, maximum 68.0
Blowdown 102.0
Safety factor 20%
Pure steam generator 1100
Pure Steam Pressure 2.4
Blowdown percentage 15%
Minimum load 35%
1500L Mammalian Purification Process Suite 1 Starts on Monday
1 2
0 0
352.27 352.27 35
35.227 35.227 35
387.5 387.5 38
445.63 445.63 44
3.00 3.01 3
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H
Sizing Assumptions
Suite 1 Media Prep
Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
Rooms (max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture SIP: 0.0803 kg/liter
M1-P1 Mam Suite 1, V+ Purification SIP: 0.0803 kg/liter
M1-P2 Mam Suite 1, V- Purification SIP: 0.0803 kg/liter Mam Suite 1 Buffer Preparation SIP: 0.0803 kg/liter
FF-Auto Filling Suite Autoclave 230 kg/load
End user load excluding HVAC
AHU (humidification)
Line Loss 10%
Total Pure Steam Usage kg/hr
USP Water Supply, LPH 782.285775
Time (Day. Hour)
Time-averaged production 407.5
Maximum Pure Steam Load 680
Line loss, maximum 68.0
Blowdown 102.0
Safety factor 20%
Pure steam generator 1100
Pure Steam Pressure 2.4
Blowdown percentage 15%
Minimum load 35%
1500L Mammalian Purification Process Suite 1 Starts on Monday
1 2 3
0 0 0
352.27 352.27 352.27
35.227 35.227 35.227
387.5 387.5 387.5
445.63 445.63 445.6
4.00 4.01 4.02
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Hi
Sizing Assumptions
Suite 1 Media Prep
Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
Rooms (max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture SIP: 0.0803 kg/liter
M1-P1 Mam Suite 1, V+ Purification SIP: 0.0803 kg/liter
M1-P2 Mam Suite 1, V- Purification SIP: 0.0803 kg/liter
Mam Suite 1 Buffer Preparation SIP: 0.0803 kg/liter
FF-Auto Filling Suite Autoclave 230 kg/load
End user load excluding HVAC
AHU (humidification)
Line Loss 10%
Total Pure Steam Usage kg/hr
USP Water Supply, LPH 782.285775
Time (Day. Hour)
Time-averaged production 407.5
Maximum Pure Steam Load 680
Line loss, maximum 68.0Blowdown 102.0
Safety factor 20%
Pure steam generator 1100
Pure Steam Pressure 2.4
Blowdown percentage 15%
Minimum load 35%
1500L Mammalian Purification Process Suite 1 Starts on Monday
1 2 3
0 0 0
352.27 352.27 352.27
35.227 35.227 35.227
387.5 387.5 387.5
445.63 445.63 445.63
5.00 5.01 5.02
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H
Sizing Assumptions
Suite 1 Media Prep
Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
Rooms (max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture SIP: 0.0803 kg/liter
M1-P1 Mam Suite 1, V+ Purification SIP: 0.0803 kg/liter
M1-P2 Mam Suite 1, V- Purification SIP: 0.0803 kg/liter Mam Suite 1 Buffer Preparation SIP: 0.0803 kg/liter
FF-Auto Filling Suite Autoclave 230 kg/load
End user load excluding HVAC
AHU (humidification)
Line Loss 10%
Total Pure Steam Usage kg/hr
USP Water Supply, LPH 782.285775
Time (Day. Hour)
Time-averaged production 407.5
Maximum Pure Steam Load 680
Line loss, maximum 68.0
Blowdown 102.0
Safety factor 20%
Pure steam generator 1100
Pure Steam Pressure 2.4
Blowdown percentage 15%
Minimum load 35%
1500L Mammalian Purification Process Suite 1 Starts on Monday
1 2 3
0 0 0
352.27 352.27 352.27
35.227 35.227 35.227
387.5 387.5 387.5
445.63 445.63 445.63
6.00 6.01 6.02
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His
Sizing Assumptions
Suite 1 Media Prep
Suite 1 Buffer Prep
500 L Mammalian Cell Culture Process Suite 2 Starts on Monday
Use points
Rooms (max. consumption)
Building 1C
M1-CC-1 Mam Suite 1, Cell Culture SIP: 0.0803 kg/liter
M1-P1 Mam Suite 1, V+ Purification SIP: 0.0803 kg/liter
M1-P2 Mam Suite 1, V- Purification SIP: 0.0803 kg/liter
Mam Suite 1 Buffer Preparation SIP: 0.0803 kg/liter FF-Auto Filling Suite Autoclave 230 kg/load
End user load excluding HVAC
AHU (humidification)
Line Loss 10%
Total Pure Steam Usage kg/hr
USP Water Supply, LPH 782.285775
Time (Day. Hour)
Time-averaged production 407.5
Maximum Pure Steam Load 680
Line loss, maximum 68.0Blowdown 102.0
Safety factor 20%
Pure steam generator 1100
Pure Steam Pressure 2.4
Blowdown percentage 15%
Minimum load 35%
1500L Mammalian Purification Process Suite 1 Starts on Monday
1 2 3
0 0 0
352.27 352.27 352.27
35.227 35.227 35.227
387.5 387.5 387.5
445.63 445.63 445.63
7.00 7.01 7.02
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Histogram of
Sizing Assumptions 1 2 3 4 5
Suite 1 biowaste 2957 70
Total biowaste liter per hour 0 0 0 0 70
Time (Day. Hour) 1.00 1.01 1.02 1.03 1.04
Tank Volume @ End of Hour 0 0 0 0 0
Throughput rate 1800 1800 1800 1800 1800
Tank Working Volume 2500 2500 2500 2500 2500
% of working volume 46% 0.00% 0.00% 0.00% 0.00% 0.00%
User Selectable
Initial Tank Volume 2500 L
Make-Up Rate 1800 Lph
Tank Working Volume 2500 L
Max inflow 2957 Lph
49.28 liter/min
4
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Histogram of C
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 46%
User Selectable
Initial Tank Volume 2500
Make-Up Rate 1800
Tank Working Volume 2500
Max inflow 2957
49.28
1 2 3 4 5
0 0 0 0 0
2.00 2.01 2.02 2.03 2.04
0 0 0 0 0
1800 1800 1800 1800 1800
2500 2500 2500 2500 2500
0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of C
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 46%
User Selectable
Initial Tank Volume 2500
Make-Up Rate 1800
Tank Working Volume 2500
Max inflow 2957
49.28
1 2 3 4 5
0 0 0 0 0
3.00 3.01 3.02 3.03 3.04
0 0 0 0 0
1800 1800 1800 1800 1800
2500 2500 2500 2500 2500
0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of C
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 46%
User Selectable
Initial Tank Volume 2500
Make-Up Rate 1800
Tank Working Volume 2500
Max inflow 2957
49.28
1 2 3 4 5
0 0 0 0 0
4.00 4.01 4.02 4.03 4.04
0 0 0 0 0
1800 1800 1800 1800 1800
2500 2500 2500 2500 2500
0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of C
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 46%
User Selectable
Initial Tank Volume 2500
Make-Up Rate 1800
Tank Working Volume 2500
Max inflow 2957
49.28
1 2 3 4 5
0 0 0 0 0
5.00 5.01 5.02 5.03 5.04
0 0 0 0 0
1800 1800 1800 1800 1800
2500 2500 2500 2500 2500
0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of C
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 46%
User Selectable
Initial Tank Volume 2500
Make-Up Rate 1800
Tank Working Volume 2500
Max inflow 2957
49.28
1 2 3 4 5
0 0 0 0 0
6.00 6.01 6.02 6.03 6.04
0 0 0 0 0
1800 1800 1800 1800 1800
2500 2500 2500 2500 2500
0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of C
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 46%
User Selectable
Initial Tank Volume 2500
Make-Up Rate 1800
Tank Working Volume 2500
Max inflow 2957
49.28
1 2 3 4 5
0 0 0 0 0
7.00 7.01 7.02 7.03 7.04
0 0 0 0 0
1800 1800 1800 1800 1800
2500 2500 2500 2500 2500
0.00% 0.00% 0.00% 0.00% 0.00%
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0
200
400
600
800
1000
1200
1400
1600
1800
2000
Biowaste,liters
Time (Day. Hour)
Cell Culture Biowaste Surge and Throughput Th
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Histo
Sizing Assumptions 1 2 3 4 5 6
Suite 1 biowaste 2957 0 0 0 0 70 1153
Total biowaste liter per hour 0 0 0 0 70 1153
Time (Day. Hour) 1.00 1.01 1.02 1.03 1.04 1.05
Tank Volume @ End of Hour 0 0 0 0 0 0Throughput rate 1800 1800 1800 1800 1800 1800
Tank Working Volume 2000 2000 2000 2000 2000 2000
% of working volume 58% maximum 0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
Ave
User Selectable Max
Initial Tank Inventory 0 L
Make-Up Rate 1800 Lph 30 Liters/min
Tank Working Volume 2000 L
Maximum Inflow 2957 Lph 49.2833 Liters/min
Time-averaged inflow 49.1 Lph 0.81776 Liters/min
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Histo
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of HourThroughput rate
Tank Working Volume
% of working volume 58% maximum
User Selectable
Initial Tank Inventory 0
Make-Up Rate 1800
Tank Working Volume 2000
Maximum Inflow 2957
Time-averaged inflow 49.1
1 2 3 4 5 6
0 0 0 0 0 0
0 0 0 0 0 0
2.00 2.01 2.02 2.03 2.04 2.05
0 0 0 0 0 01800 1800 1800 1800 1800 1800
2000 2000 2000 2000 2000 2000
0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histo
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of HourThroughput rate
Tank Working Volume
% of working volume 58% maximum
User Selectable
Initial Tank Inventory 0
Make-Up Rate 1800
Tank Working Volume 2000
Maximum Inflow 2957
Time-averaged inflow 49.1
1 2 3 4 5 6
0 0 0 0 0 0
0 0 0 0 0 0
3.00 3.01 3.02 3.03 3.04 3.05
0 0 0 0 0 01800 1800 1800 1800 1800 1800
2000 2000 2000 2000 2000 2000
0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histo
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of HourThroughput rate
Tank Working Volume
% of working volume 58% maximum
User Selectable
Initial Tank Inventory 0
Make-Up Rate 1800
Tank Working Volume 2000
Maximum Inflow 2957
Time-averaged inflow 49.1
1 2 3 4 5 6
0 0 0 0 0 0
0 0 0 0 0 0
4.00 4.01 4.02 4.03 4.04 4.05
0 0 0 0 0 01800 1800 1800 1800 1800 1800
2000 2000 2000 2000 2000 2000
0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histo
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of HourThroughput rate
Tank Working Volume
% of working volume 58% maximum
User Selectable
Initial Tank Inventory 0
Make-Up Rate 1800
Tank Working Volume 2000
Maximum Inflow 2957
Time-averaged inflow 49.1
1 2 3 4 5 6
0 0 0 0 0 0
0 0 0 0 0 0
5.00 5.01 5.02 5.03 5.04 5.05
0 0 0 0 0 01800 1800 1800 1800 1800 1800
2000 2000 2000 2000 2000 2000
0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histo
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of HourThroughput rate
Tank Working Volume
% of working volume 58% maximum
User Selectable
Initial Tank Inventory 0
Make-Up Rate 1800
Tank Working Volume 2000
Maximum Inflow 2957
Time-averaged inflow 49.1
1 2 3 4 5 6
0 0 0 0 0 0
0 0 0 0 0 0
6.00 6.01 6.02 6.03 6.04 6.05
0 0 0 0 0 01800 1800 1800 1800 1800 1800
2000 2000 2000 2000 2000 2000
0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histo
Sizing Assumptions
Suite 1 biowaste 2957
Total biowaste liter per hour
Time (Day. Hour)
Tank Volume @ End of HourThroughput rate
Tank Working Volume
% of working volume 58% maximum
User Selectable
Initial Tank Inventory 0
Make-Up Rate 1800
Tank Working Volume 2000
Maximum Inflow 2957
Time-averaged inflow 49.1
1 2 3 4 5 6
0 0 0 0 0 0
0 0 0 0 0 0
7.00 7.01 7.02 7.03 7.04 7.05
0 0 0 0 0 01800 1800 1800 1800 1800 1800
2000 2000 2000 2000 2000 2000
0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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0
250
500
750
1000
1250
1500
1750
2000
2250
2500
Inventory
,liters
Time (Day. Hour)
Biowaste (Kill System) Volume and Throughput Th
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Histogram of Process Waste
Sizing Assumptions 1 2 3 4 5 6 7
Suite 1 biowaste 2957 0 0 0 0 70 1413 1413
Fill/finish 1459
Clean steam condensate 266.135 0 0 0 0 70 0 0
Total process waste liter per hour 0 0 0 0 140 1413 1413
Time (Day. Hour) 1.00 1.01 1.02 1.03 1.04 1.05 1.06
Tank Volume @ End of Hour 0 0 0 0 0 0 0
Throughput rate 1800 1800 1800 1800 1800 1800 1800
Tank Working Volume 2250 2250 2250 2250 2250 2250 2250
% of working volume 51% maximum 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
Average Dai
User Selectable Maximum Da
Initial Tank Inventory 0 L
Throughput Rate 1800 Lph 30 Liters/min
Tank Working Volume 2250 L
Maximum Inflow 2957 Lph 49.283 Liters/min
Time-averaged inflow 210.7 Lph 3.5121 Liters/min
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Histogram of Process Waste
Sizing Assumptions
Suite 1 biowaste 2957
Fill/finish 1459
Clean steam condensate 266.135
Total process waste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 51% maximum
User Selectable
Initial Tank Inventory 0
Throughput Rate 1800
Tank Working Volume 2250
Maximum Inflow 2957
Time-averaged inflow 210.7
1 2 3 4 5 6 7
0 0 0 0 0 0 0
0 0 0 0 0 0 64.24
0 0 0 0 0 0 64.24
2.00 2.01 2.02 2.03 2.04 2.05 2.06
0 0 0 0 0 0 0
1800 1800 1800 1800 1800 1800 1800
2250 2250 2250 2250 2250 2250 2250
0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of Process Waste
Sizing Assumptions
Suite 1 biowaste 2957
Fill/finish 1459
Clean steam condensate 266.135
Total process waste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 51% maximum
User Selectable
Initial Tank Inventory 0
Throughput Rate 1800
Tank Working Volume 2250
Maximum Inflow 2957
Time-averaged inflow 210.7
1 2 3 4 5 6 7
0 0 0 0 0 0 0
0 0 0 0 0 230 64.24
0 0 0 0 0 230 64.24
3.00 3.01 3.02 3.03 3.04 3.05 3.06
0 0 0 0 0 0 0
1800 1800 1800 1800 1800 1800 1800
2250 2250 2250 2250 2250 2250 2250
0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of Process Waste
Sizing Assumptions
Suite 1 biowaste 2957
Fill/finish 1459
Clean steam condensate 266.135
Total process waste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 51% maximum
User Selectable
Initial Tank Inventory 0
Throughput Rate 1800
Tank Working Volume 2250
Maximum Inflow 2957
Time-averaged inflow 210.7
1 2 3 4 5 6 7
0 0 0 0 0 0 0
0 0 0 0 0 0 0
0 0 0 0 0 0 0
4.00 4.01 4.02 4.03 4.04 4.05 4.06
0 0 0 0 0 0 0
1800 1800 1800 1800 1800 1800 1800
2250 2250 2250 2250 2250 2250 2250
0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of Process Waste
Sizing Assumptions
Suite 1 biowaste 2957
Fill/finish 1459
Clean steam condensate 266.135
Total process waste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 51% maximum
User Selectable
Initial Tank Inventory 0
Throughput Rate 1800
Tank Working Volume 2250
Maximum Inflow 2957
Time-averaged inflow 210.7
1 2 3 4 5 6 7
0 0 0 0 0 0 0
0 0 0 0 0 0 0
0 0 0 0 0 0 0
5.00 5.01 5.02 5.03 5.04 5.05 5.06
0 0 0 0 0 0 0
1800 1800 1800 1800 1800 1800 1800
2250 2250 2250 2250 2250 2250 2250
0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of Process Waste
Sizing Assumptions
Suite 1 biowaste 2957
Fill/finish 1459
Clean steam condensate 266.135
Total process waste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 51% maximum
User Selectable
Initial Tank Inventory 0
Throughput Rate 1800
Tank Working Volume 2250
Maximum Inflow 2957
Time-averaged inflow 210.7
1 2 3 4 5 6 7
0 0 0 0 0 0 0
0 0 0 0 0 0 0
0 0 0 0 0 0 0
6.00 6.01 6.02 6.03 6.04 6.05 6.06
0 0 0 0 0 0 0
1800 1800 1800 1800 1800 1800 1800
2250 2250 2250 2250 2250 2250 2250
0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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Histogram of Process Waste
Sizing Assumptions
Suite 1 biowaste 2957
Fill/finish 1459
Clean steam condensate 266.135
Total process waste liter per hour
Time (Day. Hour)
Tank Volume @ End of Hour
Throughput rate
Tank Working Volume
% of working volume 51% maximum
User Selectable
Initial Tank Inventory 0
Throughput Rate 1800
Tank Working Volume 2250
Maximum Inflow 2957
Time-averaged inflow 210.7
1 2 3 4 5 6 7
0 0 0 0 0 0 0
0 0 0 0 0 0 0
0 0 0 0 0 0 0
7.00 7.01 7.02 7.03 7.04 7.05 7.06
0 0 0 0 0 0 0
1800 1800 1800 1800 1800 1800 1800
2250 2250 2250 2250 2250 2250 2250
0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00%
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0
250
500
750
1000
1250
1500
1750
2000
2250
2500
Inventory,
liters
Time (Day. Hour)
Process Waste Volume and Throughput Th
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WPU Correl'n WFI Correl'n Gal/min
25 647 294 294 353 353 8 29.41
30 688 313 313 375 375 8 31.27
60 867 394 394 473 473 10 39.40
70 912 415 415 497 497 11 41.4875 934 425 424 509 509 11 42.44
100 1028 467 467 561 560 12 46.71
150 1176 535 535 641 641 14 53.49
200 1294 588 589 706 706 16 58.86
250 1394 634 634 760 761 17 63.40
300 1482 674 674 808 808 18 67.37
350 1560 709 709 851 851 19 70.92
400 1632 742 742 890 890 20 74.15
450 1697 771 771 926 925 20 77.11
500 1758 799 799 959 958 21 79.88
600 1868 849 849 1019 1019 22 84.88
700 1966 894 894 1072 1072 24 89.36
750 2012 915 915 1097 1097 24 91.44
800 2056 935 935 1121 1121 25 93.43
1600 2590 1177 1177 1413 1413 31 117.7
USP PW Rinse WFI Final Rinse
Tank volume/water volum Tank volume/water volume
correlation correlation
Power Fit: y=ax^b Power Fit: y=ax^b
Coefficient Data: Coefficient Data:
a = 100.6452 a = 120.6076
b = 0.333367 b = 0.333541
2:1 fermentors/bioreactors
USP PW WFI Final Rinse, L
Total CIP
Vessel
Volume, L
CIP Fluid,
L
(for waste
NOTE: CIP volumes are based on an assumption of a flowrate of 3.46 liters/minute per metcycle time of 22 minutes is assumed, broken down as follows:
Initial rinse 2 minutes USP PWCaustic wash 2 minutes USP PWSecond rinse 4 minutes USP PW
Acid wash 2 minutes USP PWFinal rinse 12 minutes WFI