Verification: YES or NO

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Molecular diagnostics in developing countries

Ole Søgaard Lund

Verification: YES or NO

Fungi

Bacteria

Viruses

x 100

x 1000

x 10.000

Fungi

Bacteria

Viruses

ELISA

Antibodies

Fungi

Bacteria

Viruses

ELISA

Lateral Flow Detection kit

ELISA

Electricity Clean water Some Logistics Some money

PCR

PCR

Polymerase Chain Reaction

DNA

Primer (forward)

Primer (reverse)

DNA polymerase

DNA polymerase

3. round

4. round

4. round

910 nt

4. round

910 nt

+_

Agarose gel

30. round

910 nt

1000 nt

500 nt

Ethidium bromideUV-light

PCR machine

- 20 C

Electricity Clean water Some LogisticsSome money

PCR

Snail mail

Whash

PCR

+- +- +

PSbMV Tomato Canker

Verification: YES or NO

Electricity Clean water Some LogisticsSome money

PCR

Ole Søgaard Lund

Carmen Nieves Mortensen

Xili Liu