UBC Engineering Physics UBC Applied Biophysics Laboratory

½ of these samples failed:

UBC Engineering Physics UBC Applied Biophysics Laboratory

Andre MarzialiApplied Biophysics Laboratory

Engineering PhysicsDepartment of Physics and Astronomy

University of British Columbia

Beyond CSIUltra-sensitive DNA analysis for

forensics and clinical diagnostics

UBC Engineering Physics UBC Applied Biophysics Laboratory

Copyright © 2005 BrightSide Technologies Inc.

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

Synchronous Coefficient of Drag Alteration: SCODA

UBC Engineering Physics UBC Applied Biophysics Laboratory

http://www.umich.edu/~morgroup/hsvm.html

λ phage DNA in gel

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

E field

E field

kEE += 0)( μμ

Evrr μ=

Ex

Ey

electrode

electrode

electrode

electrode

1 2

UBC Engineering Physics UBC Applied Biophysics Laboratory

ExEy

electrode

electrode

electrode

electrode

1 2

UBC Engineering Physics UBC Applied Biophysics Laboratory

Ex

Ey

electrode

electrode

electrode

electrode

1 2

UBC Engineering Physics UBC Applied Biophysics Laboratory

Ex

Ey

electrode

electrode

electrode

electrode

1 2

UBC Engineering Physics UBC Applied Biophysics Laboratory

)2cos(21

21)(cos2 ωω +=

Assume a quadratic velocity term

… and a rotating driving field:

( )2ˆ Ekv E=r

( )tEEx ωcos= ( )tEEy ωsin=

( )[ ] xx EtkEv d2cos2

d2cos

ωω=

⎥⎥⎦

⎤

⎢⎢⎣

⎡⎟⎟⎠

⎞⎜⎜⎝

⎛+⎟

⎟⎠

⎞⎜⎜⎝

⎛+= y

yxx

xx E

EEE

EE

EEkv ddd

2

UBC Engineering Physics UBC Applied Biophysics Laboratory

( )txEE qx ω2cosdd −= ( )tyEE qy ω2cosdd =

2cos2(x) = 1 + cos(2x) rv rr

4d

d qEkE−=

The quadratic term in the velocity generates a 2nd harmonic of ω:

So we beat this 2nd harmonic with a perturbing field at frequency 2ω:

Most molecules: DNA and RNA:

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

DNA Concentration

A. Marziali, J. Pel, D. Bizzotto, L. Whitehead, Electrophoresis 2005, 26, 82–90

1 cm

Genome BC Technology Development UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

Boreal Genomics

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

UBC Engineering Physics UBC Applied Biophysics Laboratory

Courtesy of Rob Holt, GSC

UBC Engineering Physics UBC Applied Biophysics Laboratory

1. Lysis 2. Loading

3. Electrophoresis 4. Concentrated DNAUBC Engineering Physics UBC Applied Biophysics Laboratory

Courtesy of Rob Holt, GSC

UBC Engineering Physics UBC Applied Biophysics LaboratoryUBC Engineering Physics UBC Applied Biophysics LaboratoryGenome BC Technology Development UBC Applied Biophysics Laboratory

P.S. I didn’t do much of the actual work….

http://http://www.physics.ubc.ca/~andrewww.physics.ubc.ca/~andre//

Collaborators:Lorne WhiteheadJulian DaviesRob Holt (BCCA–GSC)Vivian MiaoKaren LuHiron Poon (RCMP)

Vincent Tabard-CossaMatthew WigginCarolina TropiniDhruti TrivediNahid JethaGeorge SterlingChris Feehan

UBC Engineering Physics UBC Applied Biophysics Laboratory

But I did hire some excellent people that did:

David Broemeling Dylan Gunn Joel Pel Peter EugsterGiorgia Tropini Jason ThompsonLaura Mai … and many othersIvan ChanGareth Mercer

Boreal Genomics

Andre Marziali, Ph.D., P.Eng.Director

andre@physics.ubc.ca

UBC ENGINEERING

PHYSICS