Post on 07-Aug-2015
a
Plants from the events BS5A.1 (T2) 18, BS6H.3 (T6) 10 and BS5A.2 (T2)
19 showed high levels of resistance to pod borer (larval survival 0 - 35%
compared to 80 to 95% in Semsen; larval weights 0.00 - 1.35 mg compared
to 5.74 in Semsen. Transgenic chickpea lines with Bt Cry2Aa gene highly
effective for the control of pod borer, H. armigera, and can be used for
increasing the production and productivity of chickpea.
pCry2Aa-nptII (pBK 201) construct map.
a
0.0
10.0
20.0
30.0
40.0
50.0
60.0
70.0
80.0
90.0
100.0
La
rva
l su
rviv
al
(%)
Genotypes
Survival of neonate larvae of Helicoverpa armigera on different lines of transgenic chickpea (5 DAI).
Larval survival (%)
0.0
1.0
2.0
3.0
4.0
5.0
La
rva
l w
eig
ht
(mg
)
Genotypes
Weight gain by the neonate larvae of Helicoverpa armigera on different lines of transgenic chickpea (5 DAI).
Larval weight (mg)
Transgenic Chickpea for Controlling the Pod Borer, Helicoverpa armigera
Helicoverpa armigera is one of the most devastating pests of cotton, grain legumes, cereals, and vegetables. It causes an estimated loss of $5 billion, despite application of insecticides costing $2 billion annually in semi arid tropics (SAT).
Leaf damage by Helicoverpa armigera in transgenic (A) and non-transgenic plants (B) of chickpea.
A B
Leaf damage by Helicoverpa armigera in chickpea.
Chickpea field damaged by Helicoverpa armigera.
ICRISAT is a member of the CGIAR Consortium
HC Sharma*, Bidyut Sharmah, S Acharjee and SP Sharma, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, India. *Email: h.sharma@cgiar.org