TRANSCRIPTION IN PROKARYOTES AND EUKARYOTES; GENE REGULATION. 

Lina Marcela Laverde PreciadoMedicine Third semester

2010-2

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INTRODUCTION

Transcription process has three stages

initiation, elongation and

termination.

In the initiation and elongation are checkpoints

that regulate

GENE

EXPRESSION

NEW WAY DISEASES CAN DEVELOP:

PREVIOUSLY UNKNOWN MECHANISM DIRECTS GENE EXPRESSION IN

CELLS

The DPF3b is a protein that recognizes a gene activator chemical marks on histones in the transcription is crucial for muscle growth and heart development

Transcription is a crucial step in

gene expression

Occurs in response to physiological and environmental stimuli

Is dictated by chemical modifications of the DNA and histones.

This discovery has broad implications in the field of epigenetic to human biology in health and disease

Science of heritable changes that do not depend on the sequence of DNA bases

Brodomains are the protein domains that recognize acetylated lysine residues and are often essential for an association that may occur between proteins and histones and can rearrange the chromatin

The discovery of this new protein involved in gene

regulation is very important because if you fail to understand

the mechanisms involved in disease development can be achieved most appropriate

treatments

OBSERVATION

ROLE OF RNA POLYMERASE IN

GENE TRANSCRIPTION DEMONSTRATED

RNA polymerase plays an important role in the formation of the transcription bubble and in the process of bending the DNA to facilitate entry of DNA into the active site.

Sequence, structure and function of RNA polymerase is universally conserved from bacteria to humans, this facilitates the study of this.

The stage of transcription initiation of RNA polymerase consists of three steps

The RNA polymerase binds with transcription promoting regions of DNA

Through interactions with the RNA polymerase, the DNA helix then unwinds

Forming an open "bubble" that allows the polymerase access to the exposed DNA sequence to begin transcription.

The importance of this study is that the RNA polymerase is the

most important molecules involved in transcription and in gene expression therefore any

knowledge about this will have a great clinical importance in the

development of pathologies

OBSERVATION

MEDICAL UTILITY

The expression of our genes is given by a combination of environmental factors, feeding radiation etc, when we come to understand the mechanism of expression can create new more effective treatments and also in terms of prevention

The discovery of new regulatory proteins may serve as biomarkers for certain diseases and acquire predictive value

Lei Zeng, Qiang Zhang, SiDe Li, Alexander N. Plotnikov, Martin J. Walsh, Ming-Ming Zhou. Mechanism and regulation of acetylated histone binding by the tandem PHD finger of DPF3b. Nature, 2010; 466 (7303): 258 DOI: 10.1038/nature09139 available http://www.sciencedaily.com/releases/2010/07/100708152101.htm

Chen et al. From the Cover: Promoter melting triggered by bacterial RNA polymerase occurs in three steps. Proceedings of the National Academy of Sciences, 2010; 107 (28): 12523 DOI: 10.1073/pnas.1003533107 aviable http://www.sciencedaily.com/releases/2010/07/100715111559.htm

Martínez LM. Transcripción genética, 5. ed. Medellín: Universidad Pontificia Bolivariana; 2009. p. 103-108.

BIBLIOGRAPHY

THANKS…