Post on 17-Apr-2018
The UC Davis Mouse Biology Program: a test case in core sustainability”
GENERAL INTEREST SESSION 2: OPS MANAGEMENT: DOLLARS AND SENSE
Western Association of Core Directors UC DAVIS CONFERENCE CENTER THURSDAY, 11 SEPTEMBER 2014
…a “scientific utility”
Sustainability factors: Raison d’etre Strong foundation, rationale Location, location, location “Reverse negotiation” People are asset…diversity is strength Customer empathy Public Service Managing “up” Outdo yourself…every day The vision thing Have fun
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Pursue reproducible research and discovery using mutant mouse models
Promote training and mentoring in mutant mouse science & technologies
Provide next-generation mouse biology services and resources.
…innovator of mouse models and molecular technologies that advance human and animal health…
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Raison d’etre
Mission:
Goals:
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
…to be a global leader in precision disease modeling to guide personalized medicine
Vision:
Raison d’etre
City of Davis
University of California Davis
SFO (85 miles)
UCDMC (12 miles)
Location, location, location
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Mouse Biology Program 2795 2nd Street, Suite 400
Davis, California
Phenotyping Center CryoTank Farm (Primary)
CryoTank Farm (Secondary)
Restricted Vivarium*
Resource/Research Laboratories
Offices (*Barrier Vivarium off-site)
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
“Reverse negotiation”
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
MBP Director
Phenotyping & Analytics
Phenotyping Center
MBML
Vivaria and Veterinary Care
High-Containment
Restricted Access
Laboratory Operations
MMCL
MSCL
MTGL
MGAL
MCRL
Information Services
Informatics
Repository Information
Management
PI Concierge Services
Import/Export Services
Marketing & PR
Business Administration
People are our greatest asset… …diversity is our greatest strength
2013-2014: 131 employees (scientists, staff, and students) 63% female 46% under-represented minorities 5 persons with disabilities
People & diversity
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Town Hall Meeting: May 13, 2014
MBP Staff Survey
MBP Retreat: May 30, 2014
Town Hall Follow-up Meeting: July 15, 2014
Some outcomes so far… Re-issue of MBP Quarterly Newsletter Regular Town Hall/All Hands Meetings Laboratory Intercommunications Meetings Employee Recognition Programs Strategic Planning Exercise and more….
People & diversity
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
NIH funded activity Researcher needs Customer empathy
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
microinjection
cryopreservation/ cryorecovery
genotyping
molecular constructs a/d/e pathology
adult phenotyping
embryo phenotyping expression analysis
sequence analysis
gene targeting
rederivation/archiving
mice
sperm & embryos
ES cells
vectors
attR1
ZeoR PheS
attR2 loxP
AmpR ori
attR3 attR4
5 ’ arm 3 ’ arm
attL2 attL1 FRT
β gal neo pA
FRT
SA
loxP
attL4 attL3 CmR ccdB
ori
PGK DTA pA
KanR
attR1
ZeoR PheS
attR2 loxP
AmpR ori
attR3 attR4
5 ’ arm 3 ’ arm
attL2 attL1 FRT
β gal neo pA
FRT
SA
loxP
attL4 attL3 CmR ccdB
ori
PGK DTA pA
KanR
attR1
ZeoR PheS
attR2 loxP
AmpR ori
attR3 attR4
AmpR ori
attR3 attR4
5 ’ arm 3 ’ arm
attL2 attL1 FRT
β gal neo pA
FRT
SA
loxP attL2 attL1 FRT
β gal neo pA
FRT
SA
loxP
attL4 attL3 CmR ccdB
ori
PGK DTA pA
KanR
attL4 attL3 CmR ccdB
ori
PGK DTA pA
KanR
iPS cells
organs, tissues data, information
Customer empathy
Students (>90): Undergraduate students Graduate students Post-doctoral fellows Professional students (VetMed, Med) Clinical Residents (LAM, Path) “HELP” Program
Lectures: ETX 250 Reproductive Toxicology IMM 293 Immunological Concepts MCRTP Summer Methods Course MMI 210 Animals Models in Infectious Diseases PHR408 Mouse Models in Research PMI 280A Experimental Mouse Biology VMB 253 Toxicant and Drug Metabolism ETC 278 Forensic Science
Courses: EMMA Cryopreservation Courses Center for Genomic Pathology
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Customer empathy
0
5000
10000
15000
20000
25000
30000
35000
All MMRRC Holdings
UC Davis
All Centers
Num
ber o
f Alle
les
MMRRC Mice & Germplasm Total number = 3880
MMRRC Repository Locations University of North Carolina UC Davis (also ICSC/Data Center) The Jackson Laboratory University Missouri-Columbia
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Public Service
Phenotyping Core A
• Metabolism • Endocrinology
Phenotyping Core B
• Body Composition • Thermoregulation • Food Intake
Phenotyping Core C
• Cardiovascular Biology • Pathology
Animal Care Core
Mice for metabolic phenotyping
Created by MBP
Purchased from KOMP,
MMRRC From PI
Phenotyping Services
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Procedures CLAMS DEXA
Gut Microbiome
Public Service
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
7M + 7F Mutant Homozygous Adult Mice
Weight Curve – 4wk to 16wk
9
Combined SHIRPA & Dysmorphology
Grip strength
Open Field
Acoustic Startle/PPI
10
Calorimetry
11
Awake ECG Intraperitoneal Glucose Tolerance Test
Auditory Brain Stem Response (4)
X-ray (5+5)
Body Composition
Fundoscopy
Electroretinography
12 13 14 15
In li
fe
16
Hematology Clinical Blood Chemistry
Insulin Blood Level
Clinical Flow Cytometry Heart Weight
Gross Pathology and Tissue Collection (2+2)
Histopathology (2+2) Urinalysis Te
rmin
al
Slit Lamp Insulin Blood Level Gait Analysis Tail flick
CTXII Blood Level CTXII Urine
Level
Tissue embedding & block banking (2+2)
Mutant mice are subjected to a series of phenotyping screens that are sequentially arranged to minimize interference on subsequent assays. Tests have been selected to assess a broad range of clinically-relevant physiological and behavioral systems.
2500+ scientists
9000+ products & services
2000+ institutions
30 countries
Public Service
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Comparative analysis of average project costs for MBP services incurred by 56 principal investigators at UC Davis during 2009-2010
up to 90% savings using MBP
Managing “up”
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Gene expression analysis
Pluripotent stem cells Laser-assisted IVF
Compensatory transcriptomics
Gene function
Metabolism of gastric bypass
CRISPR/Cas9 editing Outdo yourself…every day
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
114 publications
194 grant applications
$99,000,000 extramural research
Outdo yourself…every day
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Space crunch
Sustainability
Intellectual investment
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
vision
The Vision thing
Technological evolution of (induced) mouse models
Chemical mutagenesis Radiation
Transgenesis
GH
Knockouts
ApoE
Traditional approach: induced mutant mouse model informs relevant human phenotype
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Next-generation “precision” mouse models
Disruptive approach: genetically-defined human phenotype informs relevant mouse model
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
International Knockout Mouse Consortium International Mouse Phenotyping Consortium Federation of International Mouse Resources
Have fun.
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
~500,000 ES cell clones
~58,000 tests (>2000 anesthesias)
~87,000 embryos for injection
~1,080,000 genotypes
1st CRISPR flo’x allele using 1 template
~230,000 vials germplasm
~2,320,000 mouse cages
~13,300 service “tickets”
~11,600,000 mice (circles the earth!)
Largest NIH grant portfolio at UCD (top 0.3% in nation)
Have fun.
Your next mouse model is only a mouse click away…
www.mousebiology.org mbp@ucdavis.edu @mousebiology 2795 2nd Street Suite 400 Davis, CA 95616 (530) 754-MOUSE
Mouse Biology
Program www.mousebiology.org