Single Particle Cryo -EM

Single Particle Cryo-EM

Wen Jiang

Markey Center for Structural Biology

http://jiang.bio.purdue.edu

Cryo-EM: Imaging & 3-D Reconstruction

Imaging (3D2D)

Reconstruction(2D3D)

7Å 10Å 4Å 3Å 3.5Å 5Å 20Å

P22 phage

West Nile Virus

T7 capsid IIe15 phage

T7 capsid I

resolution

T7 capsid I (asym)

e15 phage (asym)

Single Particle Cryo-EM: Jiang Group @ Purdue

Circovirus (PCV2)

Tulane Virus

DNA nano-particles

Computing Needs for Cryo-EM

Projection

2-D Alignment

3-D Reconstruction

Image Processing Tasks

Desktop

Group of Desktops

Cluster

Condor

Computing Resources

http://diagrid.org/tools/cryoem

Cryo-EM on DiaGrid: Benefits

• pre-configured installation. As a user, you don’t need to worry about software download and installation. Just focus on your science projects.

• rapid dissemination of new algorithms. Newly developed image processing algorithms can be installed here to immediately benefit all user projects.

• large computing resource. The computational resources for image processing/3-D reconstruction tasks are provided at no cost to users by DiaGrid which leverages the vast amount of computing resources at Purdue and its partner institutions.

Single Particle Cryo -EM

Documents

Transcript of Single Particle Cryo -EM

Cryo-EM: 3D Electron Microscopy

Design of a molecular support for cryo-EM structure …making cryo-EM samples. We describe an original approach to protect proteins from harmful forces during cryo-EM sample preparation

Single particle cryo-EM reconstruction of 52 kDa ... · In recent hardware developments, new apparatuses are introduced to cryo-EM, including energy filter, Cs-corrector and volta

Near-Atomic Resolution Achieved Using Cryo -EM

APPLE Picker: Automatic Particle Picking, a Low-Effort ...amits/publications/1802...APPLE Picker: Automatic Particle Picking, a Low-Effort Cryo-EM Framework Ayelet Heimowitza, Joakim

Mechanism of Vps4 hexamer function revealed by cryo-EM€¦ · alternative for cryo-EM studies of the Vps4 oligomer. Vps4 oligomers suspended in vitreous ice for cryo-EM assume a

Single-particle Cryo-EM of Biological Macromolecules

Cryo-EM Analysis Reveals New Insights into the Mechanism ...tonglab.biology.columbia.edu/Research/pc_em.pdfSaPC Shows Different Conformations on Top and Bottom Layers Cryo-EM studies

Computational methods for single-particle cryo-electron ... · Computational methods for single-particle cryo-electron microscopy Daniel Hogan and Hugo Kitano ... but easier reconstruction

CASSPER: A Semantic Segmentation based Particle Picking Algorithm for Single Particle ... · Introduction Single-particle Cryo-electron microscopy (Cryo-EM) has revolutionized the

Phase plates for cryo-EMnramm.nysbc.org/wp-content/seminars/2017/slides/phase...• Hall et al.,”Accuratemodeling of single-particle cryo-EM images quantitates the benefits expected

OFFICIAL MEETING GUIDE & EXHIBITOR INFORMATION · 2019. 10. 24. · Falcon 3EC and Glacios for cryo-EM single particle analysis Proven technology for fast 3D structures Cryo-EM structure

Computational Methods for Single-Particle Cryo-EM · Electron cryomicroscopy, three-dimensional tomographic reconstruction, statistical estimation, conformational heterogeneity, image

Introduction: Why electrons? - Getting Started in Cryo-EM

Orientation Assignment in Cryo-EM - University of Chicagogalton.uchicago.edu/.../Singer_Orientation_Assignment.pdf · 2014. 7. 24. · Orientation Assignment in Cryo-EM Amit Singer

Confidence maps: statistical inference of cryo-EM maps

Electron Microscopy (EM) Grid and Carbon film Preparation for Cryo-Electron Microscopy Cryo-EM tutorial Cryo-EM tutorial July25~29, 2005 July25~29, 2005.

Cryo-EM: 3D Electron Microscopy - Portal IFSCCryo-EM: 3D Electron Microscopy Colin Palmer CCP-EM, UK ... •Sample preparation •Data collection •Data processing •Other cryo-EM

Single-particle cryo-electron microscopy: …processing techniques. Owing to these new exciting developments, cryo-EM was selected by Nature Methods as Method of the Year 2015, and

Structure determination of a human virus by the ... · X-ray crystallography (Step21–26) Single particle cryo-EM (Step 10–20) Viral like particle preparation (Step 1–9) Fig.