Single molecule manipulation: magnetic tweezers

INESC-MN

Magnets(NdFeB)

Magnets(NdFeB)

Resolution5 nm

[ ] Seidel et al, Nature Structural & Molecular Biology 11, 838-843 (2004)

52nd MMM Conference – Tampa FL November 2007 FA-05 3/38

EcoR124I

INESC-MN53rd MMM Conference – Austin TX November 2008

CG-05Micro Fabricated Structures (Without Fluidic Chamber)Spin Valves & Magnetic Tunnel Junctions

6

Magnetic Tweezer, DNA manipulation, DNA translocation

IAC

IDC

V+, I+ V-, I-

10m

M.R.

ATP

FP6 BioMolSwitch

INESC-MN

Optical Scope – Vertical Switch

52nd MMM Conference – Tampa FL November 2007 FA-05 28/38

45 mA

IMT

0 mA

INESC-MN53rd MMM Conference – Austin TX November 2008

CG-05

7

Micro Fabricated Structures (Without Fluidic Chamber)Spin Valves & Magnetic Tunnel Junctions

INESC-MN53rd MMM Conference – Austin TX November 2008

CG-05Bead DetectionIntermixing frequency

16