Scientific Talks

in general and

SRIP

in particular.

The Ten Minute Rule

No matter how much time has been allottedImagine that you have only 10 minutes

This will force you to isolate the important information

Distill your data down to one point

Then state the significance in one sentence

This is the THM

Step 1 :

Isolate the Take Home Message (THM)

Mike Tyson vs Evander Holyfield

Janet Jackson and Justin Timberlake

Don’t Drown In Your Data

Months later, nobody will remember the details!

For example what was memorable about:

What color were her shoes?

What round?

Your data is complex

But there is one veryimportant piece of data

Isolate it!

Step 2 The Introduction

Purpose- Start from nothing and get to the THM

Remember:

Who’s your Daddy? (your audience)

Just the Facts (Don’t show off)

•Determine the “main question” or hypothesis

•Very Specific

•The answer will have broad implications and be the THM

Remember:

Who’s your Daddy?

Just the Facts

Introduction

(The question)

Conclusion

Results

(The data and interpretation)

(The THM )

Q. How much data?

Q. How many slides?

A. = T/2(rule of thumb)

For a 10 minute talk, 5 slidesFor a 30 minute talk, 15 slidesetc

The main question dictates the data you present

Data orSlide orOverhead

?YES, then keep it

NO, then toss it

Who’s your Daddy?

Does the data address the question?

Results Section

•Longest section = .7 T•Logical flow•Avoid vague statements•Draw conclusions (different from THM)

Conclusion:

Mutations in abc1 cause abnormal eye development

THM:

Eye development is regulated by ubiquitylation

Know Your Data

•Practice without it

You must know:

•What the data are•What the data mean•Where the data lead

Step 3 The Conclusion

•Recap the question

•Recap the most important conclusion(s)

•State the THM

For Clarity -The Three Tellums

1. Tellum what you’re gonna tellum

2. Then tellum

3. Then tellum what you toldum

Slides - audiovisual aids

(The important word is “aid”. Remember….this is a TALK)

Tor signaling

•Tor is a highly conserved PI3 (lipid) kinase that is a protein kinase in vitro, and presumably in vivo.•So-called because it is the “target of rapamycin”•Rapamycin is a drug that was identified from the soil of the island of “Rapa Nui” (also known as Easter Island) in a screen for anti-fungal compounds•Yeast mutants resistant to rapamycin identified three genes TOR1 TOR2 and FRP1•FRP1 encodes a homolog of FK-506 binding protein•It is rapamycin bound to FK-506 binding protein that binds to and inhibits the Tor1 and Tor2 protein kinases

Here’s an example of a really bad slide.(Nobody came to see if you can read)

YEPD YEPD + Ben

Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2

A

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

B

C WCE IgG IP Sup-IgG IP

Mad3-PrA

Mad2

Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +

1 2 3 4 5 6 7 8 9

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

D

Don’t show figures from papers with no context

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Keep it simple.This slide explains the important experiment

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Colors are not necessarily good

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Background colors are OK but be careful

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Avoid Powerpoint backgrounds orThe curtain goes down on your career

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Sometimes nice to break things up

YEPD YEPD + Ben

Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2

A

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

B

C WCE IgG IP Sup-IgG IP

Mad3-PrA

Mad2

Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +

1 2 3 4 5 6 7 8 9

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

D

VERY BUSY

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Isolate the important information (still too busy)and lots of white space

Pull down Mad3-PrA

(Blot Mad2)

WCE

SUP

Pulldown Cdc20-13Myc

Mad3

PrA

Mad2 Mad2

Cdc20

Myc

(Blot Mad2)

Sequential Immunoprecipitation

Simplify with title, graphic, essential words

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

Isolate the important data

Mad3

PrA

Mad2 Mad2

Cdc20

Myc

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

Even better

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

Mad2

Cdc20

Myc

Mad3

PrA

Mad2

Avoid Powerpoint Nonsense

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

Mad2

Cdc20

Myc

Mad3

PrA

Mad2

or find a new career

Don’t get carried away!

“Every product of genius is a product of enthusiasm”

Benjamin Disreali

The Delivery

Be yourself and for Pete’s sake RELAX!

And finally…………..

Dress for Success

Men – Business Casual

Women – Professional not Provocative

The Ten Minute Rule

No matter what the talk/seminar is:

T/2 slides

Be professional

My Take Home Message: