Resume Metode Ekstraksi mtDNA Vendi Eko Susilo...

Achmad Farajallah | Resume Metode Ekstraksi mtDNA Vendi Eko Susilo G352110071Copyright Achmad Farajallah achamad@ipb.ac.idhttp://achamad.staff.ipb.ac.id/2011/11/26/resume-metode-ekstraksi-mtdna/

Resume Metode Ekstraksi mtDNA Vendi EkoSusilo G352110071

Resume Metode Ekstraksi mtDNAoleh Vendi Eko Susilo G352110071

No Nama Hewan Sample Metode Genome Target1 Drosophila whole body?

Adultdrosophila

alkaline lysis mtDNA

2 collectedduringautopsy byDr. William(human)

Hair the silica/guanidinethiocyanate (GuSCN)

total genome

3 yeast species cells 1) boiling, 2) glass beaddisruption, and3) acommercially available kit(Gen Toru-Kun Kit/GenTLE)

total genome

4 Hornbill Feathers use a single extractionsstep with chelex as aextraction medium

total genome

5 Insect eggs QIAamp® columns(Vallencia, CA)

haploid genome, mtDNA

6 cow fecal RBB+C ?

7 bear blood Qiam kit total genome

chicken, goat,

Fecal QIAamp DNA stool total genome, include gut

cattle and pigs

purification kit bacterial consortium

Human fecal

- UltraClean Fecal DNAextraction kit- ExtractMaster Fecal DNAextraction kit

total genome, include gutbacterial consortium

FTA technology

/ 76page 14 / 76

Sumber:

1. Rapid Isolation Methodof Animal MitochondrialDNA by the AlkalineLysis Procedure. KoichiroTamura 1 and TadashiAotsuka 1': BiochemicalGenetics, Vol. 26, Nos.11/12, 1988

2. A Silica-BasedMitochondrial DNAExtraction MethodApplied to Forensic HairShafts and Teeth. Lori E.Baker,1 M.A.; William F.McCormick,1 M.S., M.D.;and Karla J. Matteson,2Ph.D. (2001)

3. Comparison of DifferentMethods for Extraction ofMitochondrial DNA fromHuman PathogenicYeasts. Yohko Yamada*,Koichi Makimura1,2,Hossain Mirhendi1,Kumiko Ueda1, YayoiNishiyama1,2, HideyoYamaguchi1,2 andMasako Osumi; Jpn. J.Infect. Dis., 55, 122-125,2002

4. DNA extraction,amplification and directsequensing from horbillfeathers. Philip A Morina,Jeane Messier, and DavidS woodroff; J.Sci.Soc.Thailand, 20 (1994)

31-345. Human and Insect

Mitochondrial DNAAnalysis fromMaggots. Jeffrey D.Wells, Ph.D.; FrancescoIntrona, Jr., M.D.;Giancarlo Di Vella, Ph.D.,M.D.; Carlo P.Campobasso, M.D.; JackHayes, Ph.D.; and FelixA. H. Sperling, Ph.D ;JOURNAL OF FORENSICSCIENCES (2001).

6. Improved extraction ofPCR-quality communityDNA from digesta andfecalsamples. Zhongtang Yuand Mark Morrison;BioTechniques36:808-812 (May 2004)

7. Techniques forapplication of faecal DNAmethods to field studiesof Ursids. S. K. WASSER,C. S. HOUSTON, G. M.KOEHLER,* G. G. CADDand S. R. FAIN; MolecularEcology 1997, 6,1091–1097

8. A rapid and low-costDNA extraction methodfor isolating Escherichiacoli DNA from animalstools. Barnard, T. G.1*,Robertson, C. A.1, Jagals,P.1 and Potgieter, N.2;African Journal ofBiotechnology Vol. 10(8),pp. 1485-1490, 21February, 2011

9. Comparison of methodsfor the extraction ofbacterial DNA fromhuman faecal samples

for analysis by real-timePCR. E.A Nelson1, 2, E.APalombo1, and S.RKnowles2; (2010).

10. 10. Application of FTAtechnology to extractionof sperm DNA frommixed body fluidscontainingsemen. Yoshihiko Fujita,Shin-ichi Kubo; Y. Fujita,S.-i. Kubo / LegalMedicine 8 (2006) 43–47

Resume MetodeEkstraksi mtDNAoleh Vendi Eko Susilo G352110071

Adultdrosophila

2 collectedduringautopsy byDr. William(human)

total genome

3 yeast species cells 1) boiling, 2) glass beaddisruption, and3) acommercially available kit(Gen Toru-Kun Kit/GenTLE)

total genome

4 Hornbill Feathers use a single extractionsstep with chelex as aextraction medium

total genome

5 Insect eggs QIAamp® columns(Vallencia, CA)

6 cow fecal RBB+C ?

7 bear blood Qiam kit total genome

8 chicken, goat,cattle and pigs

Fecal QIAamp DNA stoolpurification kit

9 Human Human fecal - UltraClean Fecal DNAextraction kit- ExtractMaster Fecal DNAextraction kit

10 human semen FTA technology haploid genome, mtDNA

Sumber:

4. DNA extraction,amplification and directsequensing from horbillfeathers. Philip A Morina,Jeane Messier, and DavidS woodroff; J.Sci.Soc.Thailand, 20 (1994)31-34

5. Human and InsectMitochondrial DNAAnalysis fromMaggots. Jeffrey D.Wells, Ph.D.; Francesco

Introna, Jr., M.D.;Giancarlo Di Vella, Ph.D.,M.D.; Carlo P.Campobasso, M.D.; JackHayes, Ph.D.; and FelixA. H. Sperling, Ph.D ;JOURNAL OF FORENSICSCIENCES (2001).

9. Comparison of methodsfor the extraction ofbacterial DNA fromhuman faecal samplesfor analysis by real-timePCR. E.A Nelson1, 2, E.APalombo1, and S.RKnowles2; (2010).

10. 10. Application of FTAtechnology to extraction

of sperm DNA frommixed body fluidscontainingsemen. Yoshihiko Fujita,Shin-ichi Kubo; Y. Fujita,S.-i. Kubo / LegalMedicine 8 (2006) 43–47

Resume MetodeEkstraksi mtDNAoleh Vendi Eko Susilo G352110071

Adultdrosophila

collectedduringautopsy by

total genome

Dr. William(human)

yeast species

1) boiling, 2) glass beaddisruption, and3) acommercially available kit(Gen Toru-Kun Kit/GenTLE)

total genome

Hornbill

Feathers

use a single extractionsstep with chelex as aextraction medium

total genome

Insect

QIAamp® columns(Vallencia, CA)

Qiam kit

total genome

chicken, goat,cattle and pigs

QIAamp DNA stoolpurification kit

Human fecal

- UltraClean Fecal DNAextraction kit- ExtractMaster Fecal DNAextraction kit

FTA technology

Sumber:

4. DNA extraction,amplification and directsequensing from horbillfeathers. Philip A Morina,Jeane Messier, and DavidS woodroff; J.Sci.Soc.Thailand, 20 (1994)

31-345. Human and Insect

Mitochondrial DNAAnalysis fromMaggots. Jeffrey D.Wells, Ph.D.; FrancescoIntrona, Jr., M.D.;Giancarlo Di Vella, Ph.D.,M.D.; Carlo P.Campobasso, M.D.; JackHayes, Ph.D.; and FelixA. H. Sperling, Ph.D ;JOURNAL OF FORENSICSCIENCES (2001).

9. Comparison of methodsfor the extraction ofbacterial DNA fromhuman faecal samples

for analysis by real-timePCR. E.A Nelson1, 2, E.APalombo1, and S.RKnowles2; (2010).

10. 10. Application of FTAtechnology to extractionof sperm DNA frommixed body fluidscontainingsemen. Yoshihiko Fujita,Shin-ichi Kubo; Y. Fujita,S.-i. Kubo / LegalMedicine 8 (2006) 43–47

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