Post on 19-Jan-2016
Real-time Monitoring technology of Mass spectrometry
Speaker : Achong
Outline
IMS
PTR-MS
SIFT-MS
Application of IMS
IMS-TOF
IMS-IMS-TOF
Application of PTR-MS
PIT-MS
Real-time Monitoring Mass spectrometry
Environmental science
Biochemical and medical diagnosis
Workplace safety
Security check
EI source
Analysis of atmospheric air is especially challenging using electron ionization mass spectrometry, because the gas loading of the ion source by N2 and O2 (as well as water vapor) can be excessive.
70 ev
Real-time Monitoring MS
Soft chemical ionization
Measurement in Real-Time
Without pre-concentration system
I. IMS (ion mobility spectrometer)
Ion mobility mass spectrometry (Eiceman,1991; Baumbach, 2001)
Analytical Chemistry, A390-A397, November 1, 2004
Ion mobility (K)
Journal of Bimolecular Technique, Vol.13, Issue 2, June, 2002
Ion Mobility-Mass Spectroscopy
Witt & Bowers: JACS. 2000, 122, 3458.
Ion mobility mass spectrometry
Advantages:Multiform ion source could be applied.Some isomer could be separated.Real-time measurement
Disadvantages:Lower mass resolutionLower sensitivity to allow accurate analysis of complex mixtures.Standard is requisite
II. PTR-MS (proton-transfer reaction)
Proton-transfer reaction-mass spectrometry (Lagg A, Taucher J, Hansel A, Lindinger W. 1994.)
http://info.uibk.ac.at/c/c7/c722/umwelt/html/animation.html
proton-transfer reaction
H3O+ + R → RH+ + H20
[RH+]
= [H3O+]o(1 - e-k[R]t)
= [H30+]o[R]kt
J. Mass Spectrom Ion Processes, 173 (1998) 191-241
J. Mass Spectrom Ion Processes, 173 (1998) 191-241
J. Mass Spectrom Ion Processes, 173 (1998) 191-241
PTR mass spectrometry
Advantages:Soft ionization and low matrix effectReal-time measurementLow limit of detection (0.5 ppbv for 1-min measurements)
Disadvantages:A large number of VOCs could not be identified and
quantified.
III. SIFT-MS (selected ion flow tube)
http://www.syft.com/
Adams NG, Smith D. 1976. Spanel P ,Smith D. 1995.
SIFT mass spectrometry
Advantages:application of multiple reagent ions (H3O+, NO+ and O2+)quantification in real-time discriminate isomers in Real-Time
Disadvantages:The kinetic parameters of the reagent ion and the analyte ion must be known.
Selected ion flow tube mass spectrometry for no-line trace gas analysis David Smith and Patrik SpanelReference : Mass Spectrometry Reviews, 2005, 24, 661– 700
Application of IMS
IMS/TOF
IMS/IMS/TOF
Ion Mobility/TOFMS
Anal. Chem. 2003, 75,6202-6208
Pulse sequence of IMS & TOF
Anal. Chem. 2003, 75,6202-6208
example spectrum
Analytical Chemistry, 1998 Vol. 70, No. 11, June 1,
Anal. Chem. 2003, 75,6202-6208
collision-induced dissociation
http://proteomics.med.ncku.edu.tw/proteomicsweb/Liao_94_1007.ppt
IMS/IMS/TOF
Anal. Chem. 2006, 78,4161-4174
operational mode A
Anal. Chem. 2006, 78,4161-4174
operational mode B
Anal. Chem. 2006, 78,4161-4174
operational mode C
Anal. Chem. 2006, 78,4161-4174
Application of PTRMS
PIT-MS
PIT-MS
http://cires.colorado.edu/~gouw/Instruments/PITMS/PITMS.html
PIT-MSproton-transfer ion trap-mass spectrometry
J Am Soc Mass Spectrom 2005, 16, 1316–1324
CID process on ion-trap MS
http://www.chem.agilent.com/cag/Other/IonTrap_fig2.gif
calibration measurement of the PIT-MS
J Am Soc Mass Spectrom 2005, 16, 1316–1324
single CID measurement of m/z 59
ambient air pure acetone pure propanal
J Am Soc Mass Spectrom 2005, 16, 1316–1324
CID of propanal and acetone
m/z=59 m/z=41 m/z=31
J Am Soc Mass Spectrom 2005, 16, 1316–1324