Quantifying the Amount of Glucose in a Potato

BerylliumJOSE, Williard

DAROYA, RangelUTANES, KerwinELATICO, Adam

TANCHULING, DiegoMAGNO, Gian

IntorductionThere are a lot of ways to determine the

glucose concentration of a solution…These include the use of special

indicators, titration, or osmosisBut for this particular experiment, a

different protocol has been used.

HOW DID WE DO IT??!! :O :O :O

Preparation of Solutions100mL of 1 M of sulfuric acid125 mL of 0.0025316 M of Potassium

permanganateGlucose solution (1%, 2%, 4%, 8%)Potato Solution (approximately 0.5 g of crushed

potato in 50mL of water)

Preparation of Solutions

Fig 1. Adding sulfuric acid in distilled water.

Fig 2. Stirred potassium permanganate in distilled water.

Preparation of Solutions

Fig 3. Weighing of solid glucose to be used in solutions

Fig 4. Adding distilled water to solid glucose.

Preparation of Solutions

Fig 5. Weighing of potato slices. Fig 6. Three of approximately 0.5 grams of potato slices.

Preparation of Solutions

Fig 7. Crushing 0.5 grams of potato slice using mortar and pestle.

Fig 8. Placing crushed potato in Erlenmeyer flask.

Preparation of Solutions

Fig 9. Adding distilled water to crushed potato.

Fig 10. Three potato solutions, four glucose solutions, and a potassium permanganate solution

Glucose and Potato SolutionsFive milliliters of sulfric acid was added using

syringeAfter adding sulfuric acid, two milliliters of

potassium permanganate was added (syringe)Time was recorded right after potassium

permanganate was added until color disappears from solution

Adding Sulfuric Acid

Fig 11. Adding sulfuric acid to glucose solution.

Fig 12. Adding sulfuric acid to potato solution.

Adding Potassium Permanganate

Fig 13. Adding potassium permanganate in glucose solution while timing.

RESULTS!!!!

Results

The following slide shows the reaction times taken by the solutions:

ResultsSource Weight

(g)Theoretical % Glucose

Time (s)

1/Time (1/s)

Experimental % Glucose*

C6H12O6 1 0.9901 251.8 3.971E-3 0.3629

C6H12O6 2 1.9608 110.0 9.091E-3 2.5808

C6H12O6 4 3.8462 74.64 1.340E-2 4.4464

C6H12O6 8 7.4074 53.01 1.886E-2 6.8145

Potato 0.5 N/A 27.40 3.650E-2 14.452

Potato 0.5 N/A 29.05 3.442E-2 13.555

Potato 0.5 N/A 26.07 3.836E-2 15.259

Discussion

Sources