ProStar 210/218 Solvent Delivery Modules

For ALL applications (analytical to prep)

Variety of pump head materials & configurations

ProStar 210/215 Solvent Delivery Modules

Available Pumps

Major features

•Two, three or four pump gradients

•Different sizes for different flow rates

•Different materials

•Optional piston wash

•Different pressure ratings (dependent on pump type)

ProStar 210/215 Pumps

Hydraulic design

System configurations

Electrical connections and signals

Other capabilities

User interface

Product specifications

Hydraulic Design

Ball and seat check valve

Pump head

Pump Head Materials

Stainless steel

• Good for most general applicationsTitanium

• Better for high salt content applications, superior corrosion resistance

• Perceived better for biological applications• More expensive, includes piston washPeek

• Better for high salt content applications• Perceived best for Life Science applications• More expensive, + piston wash. Limited pressure

Pump Head Feature

•Piston wash

• Either Holds liquid (water) or rigged for constant recycle

• Back of Piston is rinsed automatically when it moves back-and-forth in the water

•Only on some pump heads

•Useful when using salt mobile phases

•Perceived by customers as always good

•More expensive

Pump Head

Wash head Standard head

Wash head Standard head

10WS 10SS

Pump Heads

Pump head

•Programmable re-fill time

• 100 msec to 1 sec

•Faster gives less pulsations

•Slower gives less possibility of Priming Issues with viscous mobile phases

210 218

SS and TI PEEK SS and TI PEEK

Head (mL/min) (psi) (psi) (psi) (psi)

5 0.01-5 8700 n/a 8700 n/a

10 0.01-10 8700 4000 8700 4000

25 0.025-25 4600 4000 6000 4000

50 0.05-50 2000 2000 4000 4000

100 0.1-100 1200 1200 4000 2000

200 0.2-200 n/a n/a 3500 n/a

Pressure limits

Pressure module with pulse damper

•Flow rate dependent

• 10, 50,100 and 200 mL/min

•Monitors pressures for PMIN and PMAX calculations

•Monitors pressures for compressibility compensation

• Enter 2 parameters for any solvent

•Corrects flow rate on the piston’s initial forward movement

•Teflon Membrane Design with Hexane offering damping resistance

•Different sizes for different flow rates

Transducer Pulse Damper Design

Body

Transducer

Lock Nut

Teflon membrane

Cap

Binary and Ternary Gradient Systems

•Two or three pumps, high pressure proportioning + high pressure mixing

•Can be a mixture of 210/218 pumps

•Can be different pump heads but the flow will be limited to the smallest head

•Only need ONE pressure module/pulse damper

Ternary gradient hydraulics

Pump C

Pump B

Pump A

Injector valve

Tee

Mixer

Detector

Back pressure regulator

Flow Accuracy

Flow accuracy specification

• +/- 1% of set flow

• +/- 0.05% of maximum flow

Measured %RSD =0.35%

Absolute flow can be adjusted using compressibility compensation

Flow precision

Flow precision +/- 0.1% RSD

• Measured isocratic

– 0.045% RSD overall

– 0.02% RSD best 8 consecutive runs

• Measured gradient

– 0.1% RSD overall

– 0.04% RSD best 8 consecutive runs

Gradient delay and accuracy

Gradient linearity -100%

Gradient linearity - 10%

Proportioning accuracy and precision

Precision - 0.3% to 0.03% absolute

Accuracy - 0.5% absolute - usually on the step from 0%

Proportioning accuracy and precision

• 1% steps

Proportioning accuracy and precision

• 10% steps

Isocratic precision

Mixers

Dynamic mixer

• Always needed for 210 and 218 to keep composition mixed efficiently

• Built into 210 and 218. Turns on when pump is turned on. Dual Chamber, dual elements rotating opposite of each other.

“T” mixer

• used for ternary gradient systems before dynamic mixer

Gradient delay and accuracy - results

Gradient delay - ( mixer delay) 0.55 mL

Mixer flush out (mixer flush out) 2.22 mL

User interface

•2 line display

•Numeric keypad

•Unique Features

• Negative times

• Single point control for multi-pump and UV detector

– Advised to use controller

•Control 320 UV detector

Back Panel

Electrical Connections

Outputs

• 3 contact closures

• Injection mark

• Time programmed mark

• + 5 volts

• Analog out

– Solvent composition

– Flow

– Pressure

– Wavelength Control of 320

Electrical Connections

Inputs

• Stop (Stop pump and method)

• Hold (stop method, keep pump running)

• One 22-bit ADC for data handling in Star Workstation

Other features

•Flow programming

•100 method - unlimited lines

•Built in automation

•Failure operation - shut down method

•Control of units for pressure display (Mpa, psi, Bar)

•Solvent compressibility compensation

•Single point control of gradient

Problems

Worn Seals

Dirty Check Valves

Un-primed pump

Bad fittings

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High Pressure

Fluctuating Pressure

Low Flow

Communications problems

Customer chemistry