Schekman Lecture 3

Membrane Structure: Dynamics

T3

Whitney Smith, Geneth Chin, Summitt Liu

Two Protein LinkagesAsparagine bonded to Acetylglucosamine Serine bonded to Acetylglucosamine

Fixed Membrane ProteinsGold particles attached to ConA which attaches to Mannose which binds to proteins.

Membrane

Binding to the gold allows the position of the proteins to be seen using electron microscopy.

http://en.wikiversity.org/wiki/File:Gold_molecule-membrane.jpg

Protein Placement on Cell Membrane

1) Peripheral Protein2) Peripheral Protein3) Protein Spans across bilayer4) Integral Protein5) Peripheral Protein (to protein 4)

http://en.wikiversity.org/wiki/File:Cell_membrane_protein_diagram.jpg

SDS Gel Electrophoresis Band of larger proteins

http://en.wikipedia.org/wiki/File:SDS-PAGE.jpg

Bands of smaller proteins

Immunoblotting (Western Blot Technique)

Proteins transfer onto filter paper at the bottom of the gel

https://ww2.chemistry.gatech.edu/~lw26/bCourse_Information/4581/techniques/gel_elect/gel.jpg

Nitrocellulose Filter Paper

http://en.wikiversity.org/wiki/File:SDS-PAGE_Coomasie_stained.jpg

Hydropathy Plot for Glycophorin

http://cc.scu.edu.cn/G2S/eWebEditor/uploadfile/20120810131402735.jpg

Glycophorin

Hydropathy Plot used to gain information about structure of protein

Bacterial Photoreaction Center Crystal structure for photoreaction center

http://en.wikipedia.org/wiki/File:First_satge_sbep2.svg

http://en.wikipedia.org/wiki/File:Reactprotdatbnk.JPG

Photoreaction Center Diagram