Possible factors affecting ribosomal protein expression

1. Autogenous regulation of protein expression.

2. mRNA protein degradation.

3. mRNA secondary structure.

4. Missing factors in E.coli S-30 extract

100

75

50

37

25

15

10

S1

S2

S4

S5

S13?S16?S?

“Specialized” ribosome

GFP X W X W

Ribosome N N S N

AGUGTAUUCACAUA

Future Steps and Issues

1. Increase translation efficiency.

2. Reconstitute 30S and 50S subunits.

3. Genome organization.

4. Autogenous regulation.

4. RNA processing & modifications.