Post on 31-Mar-2018
Plant DNA digests
Plasmid DNA digests Plasmid DNA digests
Plant DNA digests CSS451 Plant DNA digests
Plant Plasmid
CSS451 digests
Plant Plasmid
Southern AnalysisOptimizing DNA digest concentrations
2 4 8 12 16 20 24 ug
Estimating Yield of Dig-labeled Probe
1:5 1:50 1:500 1:5000 1:50,000
Plasmid DNA Southern Analysis
Bt-cry52244 bp
2.0kb1.9kb
21kb
5.1kb4.9kb
A
B
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
M NT LM L235 C3 80-1
Southern Analysis
Copynumber
Integration
NT LM L23 C3 80-11 2 4 5 6 7 8 9 10 11 12 13 14 15
4.0kb
3
Northern Analysis
Southern Analysis
Non-specificBinding of probebecause wrong buffer was used.
Non-bindingOf Probebecause of poorHybridization.
Image of Whole membrane
Dig-labeled size marker
Southern Analysis:Light-exposed film
Southern Analysis:Light-exposed film
cryV-BtPVYcp
RAPD Analysis of Bt-cry5/PVYcp transgenic potatoes
1 2 3 4 5 6 7 8
1.2kb
2.2kb A
B
1 2 3 4 5 6 7 8
Southern Analysis of Bt-cry5/PVYcp transgenic potatoes:Integration
1 2 3 4 5 6 7 8
3.5kb
4.2kb
4.9kb5.1kb
Southern Analysis of Bt-cry5/PVYcp transgenic potatoes:Copy Number
1.2kb
2.2kb A
B
1 2 3 4 5 6 7 8 9
1 2 3 4 5 6 7 8 9
Northern Analysis of Bt-cry5/PVYcp transgenic potatoes:Leaf tissue
1.2kb
2.2kb A
B
1 2 3 4 5 6 7 8
1 2 3 4 5 6 7 8
Northern Analysis of Bt-cry5/PVYcp transgenic potatoes:Tuber tissue
125kD
79.6kD
1 2 3 4 5 6 7 8 9 10 11 12 13
CRYV
Western Analysis of Bt-cry5 Potatoes
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1516
500 bp
456 bp
256 bp
PVY
Cry1a
NPTII
Progeny analysis of crosses between PVYcp and Bt-transgenic potatoes:
PCR results
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1621kb
21kb
5.1kb
5.1kb
4.2kb
4.2kb
3.5kb
3.5kb
PVY
Cry1a
Progeny analysis of crosses between PVYcp and Bt-transgenic potatoes:
Southern Analysis results
Fingerprint Analysis of Potato Varieties