PhotosynthesisPhotosynthesis A definition: Photosynthesis is the process a plant uses to make food...

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PhotosynthesisPhotosynthesisPhotosynthesisPhotosynthesis

A definition:Photosynthesis is the process a plant uses to make food and

grow.

A definition:Photosynthesis is the process a plant uses to make food and

grow.

Four things are needed for photosynthesis:

Travels up from the roots

WATER

CARBON DIOXIDE

Enters the leaf through small holes on the underneath

SUNLIGHT

Gives the plant energy CHLOROPHYLL

The green stuff where the chemical reactions happen

The word and chemical equations for photosynthesis:

Carbon dioxide + water glucose + oxygen

6CO2 + 6H20 C6H12O6 + 6O2

Sunlight

Chlorophyll

Sunlight

Chlorophyll

THE SUN: MAIN SOURCE OF ENERGY FOR LIFE ON EARTHTHE SUN: MAIN SOURCE OF ENERGY FOR LIFE ON EARTH

Almost all plants are photosynthetic autotrophs, as are some bacteria and protists

Autotrophs generate their own organic matter through photosynthesisSunlight energy is transformed to energy stored in the form of chemical bonds

(a) Mosses, ferns, andflowering plants

(b) Kelp

(c) Euglena (d) Cyanobacteria

THE BASICS OF PHOTOSYNTHESIS

Photosynthesis is the process by which autotrophic organisms use light energy to make sugar and oxygen gas from carbon dioxide and water

Photosynthesis is the process by which autotrophic organisms use light energy to make sugar and oxygen gas from carbon dioxide and water

AN OVERVIEW OF PHOTOSYNTHESIS

Carbondioxide

Water Glucose Oxygengas

PHOTOSYNTHESIS

The Calvin cycle makes sugar from carbon dioxide

ATP generated by the light reactions provides the energy for sugar synthesisThe NADPH produced by the light reactions provides the electrons for the reduction of carbon dioxide to glucose

LightChloroplast

Lightreactions

Calvincycle

NADP

ADP+ P

The light reactions convert solar energy to chemical energy Produce ATP & NADPH

The light reactions convert solar energy to chemical energy Produce ATP & NADPH

AN OVERVIEW OF PHOTOSYNTHESIS

Light Energy Harvested by Plants & Other Photosynthetic Autotrophs

Light Energy Harvested by Plants & Other Photosynthetic Autotrophs

6 CO2 + 6 H2O + light energy → C6H12O6 + 6 O2

WHWHY ARY ARE PE PLANLANTS GTS GREREEN?EN?

It has to do with sunlight!

Sunlight is a form of electromagnetic energy, which travels in waves.

Electromagnetic Spectrum and Visible Light

Electromagnetic Spectrum and Visible LightGamma

rays X-rays UVInfrared &

Microwaves Radio waves

Visible light

Wavelength (nm):Distance between two waves

Different wavelengths of visible light are seen by the human eye as different colors.

WHYWHY ARE ARE PLA PLANTS NTS GREGREEN?EN?

Gammarays

X-rays UV Infrared Micro-waves

Radiowaves

Visible light

Wavelength (nm)

Sunlight minus absorbed Sunlight minus absorbed wavelengths or colors wavelengths or colors equals the apparent color equals the apparent color of an object.of an object.

The feathers of male cardinals are loaded with carotenoid pigments. These pigments absorb some wavelengths of light and reflect others.

Reflected light

Chloroplasts absorb light energy and convert it to chemical energy

Chloroplasts absorb light energy and convert it to chemical energy

LightReflected

light

Absorbedlight

Transmittedlight

Chloroplast

THE COLOR OF LIGHT SEEN IS THE COLOR NOT ABSORBED

In most plants, photosynthesis occurs primarily in the leaves, in the chloroplasts

A chloroplast contains: stroma, a fluid (site of Calvin Cycle)grana, stacks of thylakoids (site of Light reactions)

The thylakoids contain chlorophyllChlorophyll is the green pigment that captures light for photosynthesis

In most plants, photosynthesis occurs primarily in the leaves, in the chloroplasts

A chloroplast contains: stroma, a fluid (site of Calvin Cycle)grana, stacks of thylakoids (site of Light reactions)

The thylakoids contain chlorophyllChlorophyll is the green pigment that captures light for photosynthesis

Photosynthesis occurs in chloroplasts

Photosynthesis occurs in chloroplasts

The location and structure of chloroplasts

The location and structure of chloroplasts

LEAF CROSS SECTION MESOPHYLL CELL

LEAF

Chloroplast

Mesophyll

CHLOROPLAST Intermembrane space

Outermembrane

Innermembrane

ThylakoidcompartmentThylakoidStroma

Granum

StromaGrana

Chloroplasts contain several pigmentsChloroplasts contain several pigments

Chloroplast PigmentsChloroplast Pigments

Chlorophyll a: absorbs mainly violet and red light the bestChlorophyll b: absorbs blue and orange light the bestCarotenoids: absorbs blue and green best

Figure 7.7

Different pigments absorb light differently

Different pigments absorb light differently

Paper ChromatographyPaper Chromatography

A laboratory test used to separate and analyze different pigments in a leaf.

Example:https://www.pearsonsuccessnet.com/snpapp/iText/getTeacherHomepage.do?newServiceId=6000&newPageId=10100

A laboratory test used to separate and analyze different pigments in a leaf.

Example:https://www.pearsonsuccessnet.com/snpapp/iText/getTeacherHomepage.do?newServiceId=6000&newPageId=10100

Photosystems:Clusters of pigments in

thylakoid membrane

Photosystems:Clusters of pigments in

thylakoid membranePhotosystem I

- Traps light energy and transfers the light-excited electrons to an electron transport chain.

- Those excited electrons are replaced by splitting a molecule of water, which releases oxygen.

- The electron transport chain releases energy, which is used to make ATP

Photosystem II

- Produces NADPH by transferring excited electrons and hydrogen ions to NADP+.

Photosystem I

- Traps light energy and transfers the light-excited electrons to an electron transport chain.

- Those excited electrons are replaced by splitting a molecule of water, which releases oxygen.

- The electron transport chain releases energy, which is used to make ATP

Photosystem II

- Produces NADPH by transferring excited electrons and hydrogen ions to NADP+.

Light Reactions Photosystem I and Photosystem IILight Reactions Photosystem I and Photosystem II

SUMMARY: In the light reactions, electron In the light reactions, electron transport chains generate ATP, NADPH, & Otransport chains generate ATP, NADPH, & O22

Two connected photosystems collect photons of light and transfer the energy to chlorophyll electrons

The excited electrons are passed from the primary electron acceptor to electron transport chains

The light reactions convert light energy to the chemical energy of ATP and NADPH

Watch the following animation:http://www.youtube.com/watch?v=eY1ReqiYwYs

SUMMARY: In the light reactions, electron In the light reactions, electron transport chains generate ATP, NADPH, & Otransport chains generate ATP, NADPH, & O22

Two connected photosystems collect photons of light and transfer the energy to chlorophyll electrons

The excited electrons are passed from the primary electron acceptor to electron transport chains

The light reactions convert light energy to the chemical energy of ATP and NADPH

Watch the following animation:http://www.youtube.com/watch?v=eY1ReqiYwYs

Ph

oto

n

Ph

oto

n

Water-splittingphotosystem

NADPH-producingphotosystem

ATPmill

Two types of photosystems cooperate in the light reactions

Inputs:WaterSunlight energy

Outputs:OxygenATPNADPH

Two types of photosystems cooperate in the light reactions

Inputs:WaterSunlight energy

Outputs:OxygenATPNADPH

The O2 liberated by photosynthesis is made from the oxygen in water (H+ and e-)

The O2 liberated by photosynthesis is made from the oxygen in water (H+ and e-)

Plants produce OPlants produce O22 gas by gas by splitting Hsplitting H22OO

Plants produce OPlants produce O22 gas by gas by splitting Hsplitting H22OO

2 H + 1/2

Water-splittingphotosystem

Reaction-center

chlorophyll

Light

Primaryelectronacceptor

Energyto make

Electron transport chain

Primaryelectronacceptor

Primaryelectronacceptor

NADPH-producingphotosystem

Light

NADP

1

23

How the Light Reactions Generate ATP and NADPH

How the Light Reactions Generate ATP and NADPH

The production of ATP in photosynthesis

The production of ATP in photosynthesis

Thylakoidcompartment(high H+)

Thylakoidmembrane

Stroma(low H+)

Light

Antennamolecules

Light

ELECTRON TRANSPORT CHAIN

PHOTOSYSTEM II PHOTOSYSTEM I ATP SYNTHASE

A Photosynthesis Road MapA Photosynthesis Road Map

Chloroplast

Light

Stack ofthylakoids ADP

+ P

NADP

Stroma

Lightreactions

Calvincycle

Sugar used for

Cellular respiration Cellulose

Starch

Other organic compounds

Calvin CycleCalvin Cycle• Called a cycle because the starting material, RuBP, is

regenerated.

• Uses carbon from carbon dioxide, the energy from ATP, and high energy electrons and hydrogen ions from NADPH to make a small sugar named G3P.

• The plant uses G3P to make glucose and other organic molecules.

Overall input:

CO2, ATP, NADPH

Overall output:

Glucose

• Called a cycle because the starting material, RuBP, is regenerated.

• Uses carbon from carbon dioxide, the energy from ATP, and high energy electrons and hydrogen ions from NADPH to make a small sugar named G3P.

• The plant uses G3P to make glucose and other organic molecules.

Overall input:

CO2, ATP, NADPH

Overall output:

Glucose

Calvin Cycle Calvin Cycle

Watch the following animation:http://www.youtube.com/watch?v=mHU27qYJNU0&NR=1 Watch the following animation:http://www.youtube.com/watch?v=mHU27qYJNU0&NR=1

Review: Photosynthesis uses light energy to make food

molecules

Review: Photosynthesis uses light energy to make food

molecules

Light

Chloroplast

Photosystem IIElectron transport

chains Photosystem I

CALVIN CYCLE Stroma

Electrons

LIGHT REACTIONS CALVIN CYCLE

Cellular respiration

Cellulose

Starch

Other organic compounds

Light reactions use water and produce oxygen.

The Calvin Cycle uses ATP and NADPH created in the the light reactions to convert carbon dioxide to glucose.

Light reactions use water and produce oxygen.

The Calvin Cycle uses ATP and NADPH created in the the light reactions to convert carbon dioxide to glucose.

Animation is of the Calvin Cycle Note what happens to the carbon dioxide and what the end product is.

Second animation of the Calvin Cycle is very clear and even does the molecular bookkeeping for you.

Animation is of the Calvin Cycle Note what happens to the carbon dioxide and what the end product is.

Second animation of the Calvin Cycle is very clear and even does the molecular bookkeeping for you.