Post on 26-Dec-2015
Parts of a Compound Light Microscope
Mirror / Light
• the light source for a microscope, typically located in the base of the microscope.
• Most light microscopes use low voltage, halogen bulbs with continuous variable lighting control located within the base.
A. Mirror / Light
Coarse adjustment knob
• used to focus the microscope
• You use it to move the objective lenses toward or away from the specimen.
C. Course adjustment knob
Fine adjustment knob
• used to focus the microscope
• This is the knob used to fine tune the focus on the specimen.
• It is also used to focus on various parts of the specimen.
• Generally one uses the coarse focus first to get close then moves to the fine focus knob for fine tuning.
B. Fine adjustment knob
Eye piece
• The eyepiece or Ocular is what you look through at the top of the microscope.
• Most eyepieces have a magnifying power of 10x.
D. Eye piece
Nose piece
• The part of the microscope that holds the objective lenses also called a revolving nosepiece or turret.
• The objectives are exposed and are mounted so that different objectives can be selected.
• Standard objectives include 4x, 10x, 40x and 100x.
F. Nose
Objective lenses
• The primary optical lenses on a microscope.
• They range from 4x-100x and typically, include, three, four or five on lens on most microscopes.
G. Objective lenses
Stage clips
• Holds the slide in place
• The viewer is required to move the slide manually to view different sections of the specimen.
H. Stage Clips
Stage
• where the specimen to be viewed is placed.
I. Stage
Diaphragm
• controls the amount of light reaching the specimen.
J. Diaphragm
Hooke's compound microscope
• From "The Microscope and Its Revelations" Carpenter, 1891: image of Hooke's compound microscope 1665
Leeuwenhoek's simple microscope
• From "The Microscope and Its Revelations" Carpenter, 1891: images of Leeuwenhoek's simple microscope
Today we will be looking at:• Salt crystals colored thread• Wool fibers newspaper print• Cotton fibers nylon fibers• The letter “e” cork• Dust silk fibers• Volcanic ash corn starch
• You will select 5 to practice focusing & drawing (in color) under all three powers
A. Mirror / Light
D. Eye piece
C. Course adjustment knob
J. Diaphragm
I. Stage
H. Stage Clips
G. Objective lenses
E. NoseB. Fine adjustment knob