Post on 07-Aug-2020
AbD Serotec®
A Bio-Rad Company
Antibodies for Characterizing Oncoproteins & Tumor Suppressor Proteins
Oncogenes & Tumor Suppressors
Oncogenes & Tumor Suppressors
Oncoproteins
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What are oncoproteins and how to characterize them?
Cancer is a complex disease that can be triggered by a variety of causes rangingfrom oncovirus infections to genetic mutations. Exposure to carcinogens and inheritance are two of the main sources for genetic mutations and predominantly occur in seven distinct protein groups (Lodish et al. 2000):
1. Apoptosis regulators2. Cell cycle control proteins3. Cell signaling proteins4. DNA repair proteins5. Growth factors6. Growth factor receptors7. Transcription factors
Mutations in growth factors, growth factor receptors, cell signaling proteins and transcription factors tend to result in dominantly active oncogenes; a term used to describe genes, which encode a cancer-promoting oncoprotein. Most oncogenes are activated via point mutations, gene amplifications and gene translocations in a normal gene known as proto-oncogene and result in altered gene expression or protein activity levels (Lodish et al. 2000).
Featured antibodies for the analysis of oncoproteins
Mutations in growth factors, growth factor receptors, cell cycle regulators, cell signaling proteins and transcription factors tend to result in dominantly active oncogenes, which alter the balance between cell proliferation and apoptosis.
The apoptosis regulator Bcl-2 oncoprotein plays an important role in deciding whether a cell lives or dies. It acts by controlling the permeability of the outer mitochondrial membrane. Murine anti-Bcl-2 antibody (clone 100) is frequently used in Immunohistochemistry experiments and has been successfully applied to demonstrate the presence of Bcl-2 in tissues of radioresistant squamous cell carcinoma (Condon et al. 2002). Western blot analysis of Bcl-2 is also frequently performed as part of a large apoptosis panel (including other markers such as Bak, Bax and Bcl-xL).
Western blot analysis of a Jurkat cell lysate with mouse anti-human Bcl-2 antibody (MCA1550) followed by detection with a goat anti-mouse HRP conjugated secondary antibody. Visualization was performed by chemiluminescence.
Applications: Flow Cytometry, Immunohistochemistry frozen and paraffin, Immunoprecipitation and Western Blotting
Citations: 11
Find out more at: abdserotec.com/bcl2
Featured murine anti-Bcl-2 antibody (MCA1550)
The Ras protein family encompasses three members HRAS, NRAS and KRAS. The genes encoding these proteins present some of the best studied proto-oncogenes, which upon mutation are involved in inducing various cancer types (including bladder, cervical, pancreatic and prostate cancers). Murine anti-H-Ras antibody (MCA2884) is frequently used in Western Blotting experiments and has been successfully applied for the detection of H-Ras in human epithelioid breast cancer cells (MCF10A) (Vuoriluoto et al. 2011).
Western blot analysis of a HEK293 cell lysate with mouse anti- human H-Ras antibody (MCA2884) followed by detection with a goat anti-mouse HRP conjugated secondary antibody. Visualization was performed by chemiluminescence.
Applications: ELISA and Western Blotting
Citations: 12
Find out more at: abdserotec.com/hras
Featured murine anti-H-Ras antibody (MCA2884)
Oncoproteins Oncoproteins - Cell Signaling Group
AbD Serotec offers a variety of antibodies and proteins for analyzing oncoproteins. For your convenience, we have grouped our oncoprotein research reagents into the four main oncoprotein classes (cell signaling proteins, growth factors, growth factor receptors and transcription factors).
Oncoproteins - Cell Signaling Group (Antibodies)
Find out more at: abdserotec.com/oncoabs 3
Specificity Product Type Clone Species Reactivity Applications Host Format Size Product Code
AKT1 Monoclonal Antibody 4C3 Human E Mouse Preservative
Free 0.1 mg MCA2919Z
AKT1 Monoclonal Antibody 6F11 Human, Mouse, Rat IF/ICC, WB Mouse Preservative
Free 0.1 mg MCA4779Z
AKT1 Recombinant Antibody 2147 Human, Mouse, Rat E, WB HuCAL® Purified 0.1 mg HCA003
AKT1 Recombinant Antibody 3813 Human, Mouse, Rat E, WB HuCAL Purified 0.1 mg HCA017
AKT1 Polyclonal Antibody Human, Mouse, Rat WB Rabbit Purified 50 µg AHP968T
AKT1 Polyclonal Antibody Human, Mouse, Rat WB Rabbit Purified 0.1 mg AHP968
AKT2 Polyclonal Antibody 1F3 Human, Mouse, Rat E, IF/ICC, WB Mouse Preservative
Free 0.1 mg MCA5507Z
AKT2 Polyclonal Antibody 1B3 Human IF/ICC, WB Mouse Preservative
Free 0.1 mg MCA2920Z
AURORA-A Kinase
Monoclonal Antibody 35C1 Human, Mouse IF/ICC, WB Mouse Biotin 0.1 mg MCA2249B
AURORA-A Kinase
Monoclonal Antibody 35C1 Human, Mouse IF/ICC, IP, WB Mouse Purified 0.1 mg MCA2249
AURORA-A Kinase
Polyclonal Antibody Human IHC-F, IP Rabbit Purified 50 µg AHP873
AURORA-B Kinase
Polyclonal Antibody Human E, WB Rabbit Purified 50 µg AHP1261
Bax Monoclonal Antibody 1F5-1B7 Human IHC-P* Mouse Preservative
Free 0.1 mg MCA2960Z
Bax Monoclonal Antibody 2D2 Bovine, Human,
Monkey E, IF/ICC, IP, WB Mouse Purified 0.1 mg MCA2738
Bax Polyclonal Antibody Human IHC-P*, IP, WB Rabbit Serum 0.1 ml AHP471
Bax Polyclonal Antibody Human IHC-P* Rabbit Serum 20 µl AHP471T
Bcl-2 Polyclonal Antibody Human E, WB Goat Purified 0.1 mg AHP1057
Bcl-2 Monoclonal Antibody 100 Human FC*, IHC-F, IHC-P*,
IP, WB Mouse Purified 0.1 mg MCA1550
Bcl-2 Polyclonal Antibody Human E Rabbit Purified 1 ml 0300-0016
Bcl-2 Polyclonal Antibody Human E Rabbit Purified 1 ml 0300-0020
Bcl-2 Binding Component 3
Polyclonal Antibody Human IHC-P*, WB Rabbit Purified 0.1 mg AHP727
Bcl-2 Binding Component 3
Polyclonal Antibody Human IHC-P*, WB Rabbit Purified 50 µg AHP727T
HRAS Monoclonal Antibody H-Ras-03 Human, Mouse, Rat E, WB Mouse Purified 0.1 mg MCA2884
KRAS Monoclonal Antibody 4F3 Human WB Mouse Preservative
Free 0.1 mg MCA4768Z
KRAS Monoclonal Antibody 3B10-2F2 Human IF/ICC, WB Mouse Preservative
Free 0.1 mg MCA3223Z
LCK Monoclonal Antibody LCK-01 Human FC*, IHC-F, IP, WB Mouse Purified 0.1 mg MCA2380
NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse Alexa Fluor®
48825 Tests/ 0.25 ml MCA2410A488T
NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse Alexa Fluor
488100 Tests/ 1 ml MCA2410A488
NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse Alexa Fluor
647100 Tests/ 1 ml MCA2410A647
NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse FITC 0.1 mg MCA2410F
NOTCH1 Monoclonal Antibody 4G1 Human, Mouse WB Mouse Preservative
Free 0.1 mg MCA4804Z
Oncoproteins - Cell Signaling Group
Oncoproteins - Cell Signaling Group (Antibodies) Continued
Find out more at: abdserotec.com/oncoabs
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Specificity Product Type Clone Species Reactivity Applications Host Format Size Product Code
NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC*, IHC-F, WB Mouse Purified 0.1 mg MCA2410GA
NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC*, IHC-F, WB Mouse Purified 25 µg MCA2410T
NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC*, IHC-F, WB Mouse Purified 0.25 mg MCA2410
NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse RPE 100 Tests MCA2410PE
NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse RPE 25 Tests MCA2410PET
NOTCH1 Monoclonal Antibody 8G10 Mouse, Rat FC, IHC-F, WB Hamster S/N 0.1 ml OBT2045
NOTCH2 Monoclonal Antibody HMN2-35 Mouse, Rat FC Hamster Purified 0.25 mg MCA5702
NOTCH2 Monoclonal Antibody HMN2-35 Mouse, Rat FC Hamster Purified 0.1 mg MCA5702GA
NOTCH2 Monoclonal Antibody HMN2-35 Mouse, Rat FC Hamster RPE 100 Tests MCA5702PE
NOTCH2 Polyclonal Antibody Human E, IHC-P* Rabbit Serum 50 µl AHP2180
NOTCH2 Polyclonal Antibody
Dog, Human, Mouse, Rat E, WB Rabbit Serum 50 µl AHP1289
NOTCH3 Monoclonal Antibody HMN3-133 Mouse, Rat FC Hamster Purified 0.25 mg MCA5703
NOTCH3 Monoclonal Antibody HMN3-133 Mouse, Rat FC Hamster Purified 0.1 mg MCA5703GA
NOTCH3 Polyclonal Antibody
Human, Monkey, Mouse, Rat E, IHC-P* Goat Purified 50 µg AHP2182
NOTCH4 Monoclonal Antibody HMN4-14 Mouse FC Hamster Purified 0.25 mg MCA5704
NOTCH4 Monoclonal Antibody HMN4-14 Mouse FC Hamster Purified 0.1 mg MCA5704GA
NOTCH4 Monoclonal Antibody HMN4-14 Mouse FC Hamster RPE 100 Tests MCA5704PE
PIM-1 Monoclonal Antibody 2C9 Human IHC-P Mouse Preservative
Free 0.1 mg MCA5346Z
RAF-1 Monoclonal Antibody Roy/28/1 Human WB* Rat Purified 0.1 mg MCA2174
RAF-1 Polyclonal Antibody
Bovine, Chicken, Dog, Human, Primate WB* Rabbit Purified 0.1 ml AHP1237
RAF-1 pS338 Monoclonal Antibody DPR/2/2A/3A Human WB* Rat Purified 0.1 mg MCA1852
WNT-1 Polyclonal Antibody Human E, IHC-P*, WB Rabbit Purified 0.1 mg AHP1287
Oncoproteins - Cell Signaling Group Oncoproteins - Growth Factor Group
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Oncoproteins - Growth Factor Group (Antibodies & Proteins)
Find out more at: abdserotec.com/oncoabs
Specificity Product Type Clone Species Reactivity Applications Host Format Size Product Code
EGF Receptor Polyclonal Antibody Mouse E, WB Goat Purified 0.1 mg AAM52
EGF Receptor Polyclonal Antibody Rat E, FN, WB Rabbit Purified 0.1 mg AAR37
EGF Receptor Polyclonal Antibody Human E, FN, IHC-P*, WB Rabbit Purified 0.1 mg AHP767
EGF Receptor Polyclonal Antibody Human E, WB Rabbit Biotin 50 µg AHP767B
EGF Receptor Protein Mouse FN N/A Purified 1 mg EGF1
EGF Receptor Rec. Protein Human E, FN, WB N/A Rec. Protein 0.5 mg PHP030A
EGF Receptor Rec. Protein Mouse FN N/A Rec. Protein 0.5 mg PMP69
EGF Receptor Rec. Protein Rat FN N/A Rec. Protein 0.1 mg PRP31
FGF 4 Rec. Protein Human FN N/A Rec. Protein 25 µg PHP204
FGF 8 Rec. Protein Human FN N/A Rec. Protein 25 µg PHP130
FGF 9 Polyclonal Antibody Mouse E, WB Rabbit Biotin 50 µg AAM17B
FGF 9 Polyclonal Antibody Mouse E, FN, WB Rabbit Purified 0.1 mg AAM17G
FGF 9 Rec. Protein Mouse FN N/A Rec. Protein 10 µg PMP61
FGF 10 Polyclonal Antibody Human E, IHC-P*, WB Goat Purified 0.1 mg AHP1745
PDGF Monoclonal Antibody A04-5D9 Human E Mouse Purified 0.1 mg MCA5989GA
PDGF Polyclonal Antibody Human E, FN, IHC-P*, WB Rabbit Purified 0.1 mg 7460-0635
PDGF Polyclonal Antibody Human E, FN, IHC-P*, WB Rabbit Purified 0.1 mg AHP792
PDGF Polyclonal Antibody Human E, FN, IHC-P*, WB Rabbit Purified 0.1 mg AHP793
PDGF Rec. Protein Human E, FN, WB N/A Rec. Protein 10 µg PHP036
PDGF Rec. Protein Human FN N/A Rec. Protein 10 µg PHP035
VEGF Monoclonal Antibody 4B2-8 Human E, FN, WB Mouse Preservative
Free 0.5 mg MCA5750Z
VEGF Monoclonal Antibody CH-10 Human, Mouse,
Rat WB Mouse Purified 0.1 mg MCA5693
VEGF Polyclonal Antibody Mouse E, FN, IF/ICC, IHC-P*,
WB Rabbit Purified 0.1 mg AAM51
VEGF Polyclonal Antibody Mouse E, WB Rabbit Biotin 50 µg AAM51B
VEGF Polyclonal Antibody Rat E, FN, WB Rabbit Purified 0.1 mg AAR42
VEGF Polyclonal Antibody Rat E, WB Rabbit Biotin 50 µg AAR42B
VEGF Polyclonal Antibody Human E, FN, IHC-P*, WB Goat Purified 0.1 mg AHP831
VEGF Polyclonal Antibody Human E, WB Goat Biotin 50 µg AHP831B
VEGF Rec. Protein Human E, FN, WB N/A Rec. Protein 10 µg PHP091
VEGF Rec. Protein Mouse E, FN, WB N/A Rec. Protein 10 µg PMP67
VEGF Rec. Protein Rat FN N/A Rec. Protein 10 µg PRP35
Oncoproteins - Growth Factor Receptor Group
Oncoproteins - Growth Factor Receptor Group (Antibodies)
Find out more at: abdserotec.com/oncoabs
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Specificity Product Type Clone SpeciesReactivity Applications Host Format Size Product Code
EGF Receptor Monoclonal Antibody ICR10 Human FC, IHC-F, IHC-P, IP Rat Purified 0.2 mg MCA1784
EGF Receptor Monoclonal Antibody ICR10 Human FC Rat FITC 0.1mg MCA1784F
EGF Receptor Monoclonal Antibody ICR10 Human FC, IHC-F, IHC-P, IP Rat Purified 1 mg MCA1784G
EGF Receptor Monoclonal Antibody ICR10 Human FC Rat RPE 100 Tests MCA1784PE
EGF Receptor Monoclonal Antibody F4 Human IHC-F* Mouse FITC 0.1 mg MCA455FT
EGF Receptor Monoclonal Antibody G02-2D5 Human E, WB Mouse Purified 0.1 mg MCA5986GA
EGF Receptor Polyclonal Antibody Human IHC-P* Rabbit Purified 50 µg AHP1372
EGF Receptor Monoclonal Antibody 1E4 Human E, FC Sheep Purified 0.5 mg MCA2198
EGF Receptor Monoclonal Antibody C1.1 Human E, FC Sheep Purified 0.5 mg MCA2199
EGF Receptor Monoclonal Antibody ICR11 Human FC, IHC-F, IP Rat Purified 0.2 mg MCA1785
EGF Receptor Monoclonal Antibody ICR11 Human FC, IHC-F, IP Rat Purified 1 mg MCA1785G
EGF Receptor Monoclonal Antibody ICR61 Human IHC-F*, IP Rat Purified 0.2 mg MCA1789
EGF Receptor Monoclonal Antibody ICR61 Human IHC-F*, IP Rat Purified 1 mg MCA1789G
EGF Receptor Polyclonal Antibody
Human, Mouse, Rat E, WB Rabbit Purified 50 µg AHP1243
EGF Receptor Monoclonal Antibody F4 Human IHC-F* Mouse S/N 2 ml MCA455
ErbB3 Polyclonal Antibody
Dog, Human, Mouse, Rat E, WB Goat Purified 0.1 mg AHP1088
ErbB3 Monoclonal Antibody SGP1 Human E, FC, IHC-F*, IP Mouse Purified 0.1 mg MCA1410
ErbB4 Polyclonal Antibody Human IHC-P* Rabbit Purified 50 µg AHP1131
HER2/neu Monoclonal Antibody ICR52 Human IHC-F Rat Purified 0.2 mg MCA1787
HER2/neu Monoclonal Antibody ICR52 Human IHC-F Rat Purified 25 µg MCA1787T
HER2/neu Monoclonal Antibody ICR55 Human FC, IHC-F, IHC-P,
IP, WB Rat Purified 0.2 mg MCA1788
HER2/neu Monoclonal Antibody ICR55 Human FC Rat Alexa Fluor
700 100 Tests MCA1788A700
HER2/neu Monoclonal Antibody ICR55 Human FC, IHC-F, IHC-P,
IP, WB Rat Purified 25 µg MCA1788T
HER2/neu Polyclonal Antibody Human E, IHC-F, WB* Rabbit Serum 0.1 ml AHP478
VEGF Receptor 1 Monoclonal Antibody FLT-19 Human E, IHC-F, IP Mouse S/N 0.1 mg MCA2743
VEGF Receptor 1 Polyclonal Antibody Human E, WB Rabbit Serum 0.1 ml AHP1600
VEGF Receptor 2 Polyclonal Antibody Human IHC-P* Rabbit Purified 50 µg AHP1327
VEGF Receptor 3 Monoclonal Antibody 5B5 Human WB Mouse Preservative
Free 0.1 mg MCA5331Z
Oncoproteins - Growth Factor Receptor Group Oncoproteins - Transcription Factor Group
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Oncoproteins - Transcription Factor Group (Antibodies)
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Find out more at: abdserotec.com/oncoabs
Specificity Product Type Clone SpeciesReactivity Applications Host Format Size Product Code
c-Myc Monoclonal Antibody JAC6 Human IF/ICC, IHC-F, IP, WB Rat Purified 0.1 mg MCA1929
c-Myc Monoclonal Antibody 9E10 Human FC* Mouse Alexa Fluor
647 100 Tests MCA2200A647
c-Myc Monoclonal Antibody 9E10 Human E, IHC-F, IHC-P, WB* Mouse Alk. Phos. 0.1 mg MCA2200A
c-Myc Monoclonal Antibody 9E10 Human E, IHC-F, IHC-P, WB Mouse Biotin 0.1 mg MCA2200B
c-Myc Monoclonal Antibody 9E10 Human FC, IF Mouse Dylight® 549 0.1 mg MCA200D549GA
c-Myc Monoclonal Antibody 9E10 Human FC* Mouse FITC 0.1 mg MCA2200F
c-Myc Monoclonal Antibody 9E10 Human E, IHC-F, IHC-P, WB* Mouse HRP 0.1 mg MCA2200P
c-Myc Monoclonal Antibody 9E10 Human E, FC*, IHC-F, IHC-P,
WB* Mouse Purified 2 mg MCA2200G
c-Myc Monoclonal Antibody 9E10 Human FC* Mouse Alexa Fluor
488 100 Tests MCA2200A488
c-Myc Monoclonal Antibody 9E10 Human E, FC*, IHC-F, IHC-P,
WB* Mouse Purified 1 mg MCA2200
c-Myc Monoclonal Antibody 9E10 Human E, FC*, IHC-F, IHC-P,
WB* Mouse Purified 0.1 mg MCA2200GA
c-Myc Monoclonal Antibody 9E10 Human E, FC*, IHC-F, IHC-P,
WB* Mouse Purified 20 µg MCA2200T
c-Myc Monoclonal Antibody 9E10 Human FC* Mouse RPE 100 Tests MCA2200PE
GL12 Polyclonal Antibody Mouse, Rat E, IHC-P*, WB Rabbit Purified 50 µg AHP1455
GL13 Polyclonal Antibody
Chicken, Dog, Human, Monkey, Mouse, Rat
E, IHC-P*, WB Rabbit Purified 50 µg AHP1456
HOXA11 Monoclonal Antibody 8B8 Human IHC-P, WB Mouse Preservative
Free 0.1 mg MCA4918Z
LM02 Monoclonal Antibody 1A9-1 Human IF/ICC, IHC-P*, WB Mouse Purified 0.2 mg MCA2744
LM02 Monoclonal Antibody 1A9-1 Human IF/ICC, IHC-P*, WB Mouse Purified 0.1 mg MCA2744GA
PML Monoclonal Antibody 1D12 Human, Rat IF, WB Mouse Preservative
Free 0.1 mg MCA3345Z
SMAD2 Monoclonal Antibody 3G9 Human IF/ICC, IHC-P, WB Mouse Preservative
Free 0.1 mg MCA4935Z
SMAD2 Polyclonal Antibody Human WB Goat Purified 0.1 mg AHP694
SOX2 Monoclonal Antibody PR-4C6 Human E, WB Mouse Purified 0.1 mg MCA5659
SOX2 Monoclonal Antibody PR-4C6 Human E, WB Mouse Purified 25 µg MCA5659T
SOX2 Monoclonal Antibody PR-4E4 Human E, WB Mouse Purified 0.1 mg MCA5660
SOX2 Monoclonal Antibody PR-4E4 Human E, WB Mouse Purified 25 µg MCA5660T
SOX9 Monoclonal Antibody 3C10 Human IF, IHC-P, WB Mouse Preservative
Free 0.1 mg MCA4974Z
v-myb/A-myb Monoclonal Antibody 5E11 Human, Mouse,
Xenopus IHC-F, IP, WB Mouse Purified 0.1 mg MCA1794
v-myb/c-myb Monoclonal Antibody 4E3 Xenopus IP, WB Mouse Purified 0.1 mg MCA1792
v-myb/c-myb Monoclonal Antibody 10A3 Human, Mouse,
Xenopus, Zebrafish IP, WB Mouse Purified 0.1 mg MCA1793
Tumor Suppressors
Mutations in two gene groups, tumor suppressors and oncogenes, play an important role in inducing and promoting a large variety of cancers. Oncogenes tend to encode growth factors, growth factor receptors, cell signaling proteins and transcription factors, while tumor suppressor genes encode cell cycle regulators. Most oncogenes are activated via point mutations, gene amplifications and translocations in a normal gene known as proto-oncogene and result in altered gene expression or protein activity levels (Lodish et al. 2000). In contrast to oncogenes, mutant tumor suppressor genes are recessive. They follow the Knudson or “multiple-hit hypothesis”, which suggest that both alleles of a gene need to be mutated in order to trigger disease onset.
In spite of this hypothesis, the inheritance of certain mutant tumor suppressor alleles, such as BRCA1 and BRCA2 mutant alleles, significantly increase the risk of developing breast and ovarian cancers. For example a female carrying a mutant BRCA1 allele has a 60-90% lifetime risk of developing breast cancer and a 40-60% risk of developing ovarian cancer (NHS UK). In light of these increased risks predictive genetic testing for BRCA1, BRCA2, TP53 and PTEN mutations are available for those who have a family history of early onset breast cancer.
Featured antibodiesThe human p53 protein is a well-studied protein consisting of transactivation (AA 1-94), DNA binding (AA 94-294) and oligomerization/tetramerization (AA 323-356) domains. The transactivation domain can be further divided into transactivation domain I (AA 1-43), transactivation domain II (AA 44-63) and a proline-rich region (AA 64-94).
AbD Serotec offers a variety of antibodies for the analysis of the different human p53 domains including:
Find out more at: abdserotec.com/suppressors
Mutations in tumor suppressor genes, such as TP53, and oncogenes such as H-Ras account for the development of a large variety of cancers.
Clone Name Binding region p53 domain Product Code
DO-2 AA 10-16 Transactivation MCA1702
DO-1 AA 20-25 Transactivation MCA1701
BP53-12 AA 20-25 Transactivation OBT3175
PrecisionAb™ Monoclonal AA 20-25 Transactivation VMA00019K
DO-13 AA 26-35 Transactivation MCA1706
Pab1801 AA 46-55 Transactivation MCA577
DO-14 AA 56-65 Transactivation MCA1707
Pab246 AA 88-109 DNA binding MCA910
2C3 AA 94-202 DNA binding and oligomerization MCA3510Z
DO-11 AA 181-190 DNA binding MCA1704
Pab240 AA 213-217 DNA binding MCA909
FP3-2 pS392Oligomerization;Serine 392 becomes phosphorylated in response to UV damage
MCA1842
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Tumor Suppressors Tumor Suppressor Antibodies
The anti-p53 antibody (VMA00019K) is part of the PrecisionAb range and has been highly validated in Western Blotting. PrecisionAb products are characterized by their high specificity, high sensitivity and batch to batch consistency (every lot of antibody meets the same strict quality control criteria).
Find out more at: abdserotec.com/precisionab
Western blot analysis of whole cell lysates probed with anti-p53 antibody (VMA00019K) followed by detection with HRP conjugated goat anti-mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 2 second exposure. The arrow points to the p53 protein (molecular weight 53 kDa).
Featured PrecisionAbTM anti-p53 antibody (VMA00019K)
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Specificity Product Type Clone Species Reactivity Applications Host Format Size Product Code
BLM Polyclonal Antibody Human IP, WB Rabbit Purified 50 µg AHP875
BRCA1 Monoclonal Antibody MS110 Human
FC, IF/ICC, IHC-F*, IHC-P*, IP, WB
Mouse Purified 50 µg MCA1682GT
BRCA1 Monoclonal Antibody MS13 Human IF/ICC, IHC-P, IP,
WB Mouse Purified 50 µg MCA1683GT
BRCA1 Monoclonal Antibody BR64 Human IP, WB Mouse Purified 50 µg MCA5944GT
BRCA1 Monoclonal Antibody 6B4 Human IF/ICC, IHC-P, IP,
WB Mouse Purified 50 µg MCA5945GT
BRCA1 Monoclonal Antibody 17F8 Human E, IF/ICC, IHC-P,
IP, WB Mouse Purified 50 µg MCA5946GT
BRCA1 Monoclonal Antibody 8F7 Human IHC-F*, IHC-P*,
IP, WB Mouse Purified 50 µg MCA5947GT
BRCA1 Polyclonal Antibody Human IP, WB Rabbit Purified 50 µg AHP1470
BRCA2 Polyclonal Antibody
Cat, Dog, Human, Mouse, Rat IHC-F, IHC-P* Rabbit Purified 1 ml 0300-0023
Catenin beta Polyclonal Antibody
Dog, Human, Mouse
E, IB, IHC-F, IHC-P*, IP Rabbit Serum 50 µl OBT1683
Catenin beta Monoclonal Antibody 1C9 Human WB Mouse Preservative
Free 0.1 mg MCA4879Z
Cdk2 Monoclonal Antibody 3148 Human E, WB HuCAL Purified 0.1 mg HCA063
Cdk2 Monoclonal Antibody 3148 Human E, WB HuCAL Purified 50 µg HCA063A
Cdk2 Polyclonal Antibody Human E, IHC-P, IP, WB Rabbit Serum 50 µl AHP1247
Cdk4 Monoclonal Antibody DCS-31.2 Human, Mouse, Rat IHC-F, IP, WB Mouse Purified 0.1 mg MCA1994
CDKN2A Polyclonal Antibody Human E, WB Goat Purified 0.1 mg AHP1060
CDKN2A Polyclonal Antibody Human IHC-P*, WB Rabbit Purified 0.1 mg AHP1488
Chk1 Polyclonal Antibody Human IP, WB Goat Purified 50 µg AHP877
Chk2 Monoclonal Antibody 4B7 Human IF/ICC,IHC-P*, WB Mouse Preservative
Free 0.1 mg MCA3753Z
Cdk2 Polyclonal Antibody Human, Mouse, Rat E, WB* Rabbit Purified 50 µg AHP1266
Cyclin D1 Monoclonal Antibody CD1.1 Human, Rat E, FC*, IHC-F,
IHC-P*, IP, WB Mouse Purified 0.1 mg MCA1756
Cyclin D1 Monoclonal Antibody CD1.1 Human, Rat FC*, IHC-F, IHC-P* Mouse Purified 10 µg MCA1756T
Tumor Suppressors (Antibodies)
Find out more at: abdersotec.com/suppressorabs
Tumor Suppressor Antibodies
Tumor Suppressors (Antibodies) Continued
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Specificity Product Type Clone Species Reactivity Applications Host Format Size Product Code
Cyclin D1 Polyclonal Antibody
Dog, Human, Mouse, Rat E, WB Rabbit Serum 0.1 ml 2407-0508
Cyclin D3 Monoclonal Antibody DCS-22 Dog, Human IF/ICC IHC-F,
IHC-P*, WB Mouse S/N 1 ml MCA1866
Cyclin E Polyclonal Antibody Human, Mouse, Rat E, IP, WB Rabbit Serum 50 µl AHP1245
DNA PKcs Polyclonal Antibody
Hamster, Human, Mouse, Rat
GSA, IHC-P*, IP, WB Rabbit Serum 0.1 ml AHP318
NBS1 Polyclonal Antibody Human IP, WB Rabbit Purified 0.1 mg AHP842
p53 Polyclonal Antibody Human IHC-F, IHC-P* Rabbit Concentrate 0.1 ml AHP535T
p53 Polyclonal Antibody Human IHC-F, IHC-P* Rabbit Concentrate 1 ml AHP535
p53 Monoclonal Antibody BP53-12 Human FC* Mouse FITC 100 Tests OBT3175F
p53 Monoclonal Antibody 2C3 Human IF/ICC, IHC-P*,
WB Mouse Preservative Free 0.1 mg MCA3510Z
p53 Monoclonal Antibody PAb1801 Human IP, WB Mouse Purified 0.1 mg MCA577
p53 Monoclonal Antibody PAb240 Human IP, WB* Mouse Purified 0.1 mg MCA909
p53 Polyclonal Antibody Human, Monkey, Rat WB* Rabbit Purified 0.1 ml AHP906
p53 Polyclonal Antibody Human WB Rabbit Purified 50 µl 7045-3099
p53 Monoclonal Antibody DO-2 Human IHC-F, IHC-P*, IP,
WB Mouse Purified 0.1 mg MCA1702
p53 Monoclonal Antibody DO-2 Human IHC-F, IHC-P* Mouse Purified 20 µg MCA1702T
p53 Monoclonal Antibody DO-13 Human IHC-F, IHC-P*, IP,
WB Mouse Purified 0.1 mg MCA1706
p53 Monoclonal Antibody DO-13 Human IHC-F, IHC-P* Mouse Purified 20 µg MCA1706T
p53 Monoclonal Antibody BP53-12 Human IHC-F, IHC-P*, WB Mouse Purified 0.1 mg OBT3175
p53 Monoclonal Antibody DO-11 Human IHC-F, IHC-P*, IP,
WB Mouse Purified 0.1 mg MCA1704
p53 Monoclonal Antibody DO-14 Human IHC-F, IHC-P*, IP,
WB Mouse Purified 0.1 mg MCA1707
p53 Monoclonal Antibody FP3-2 Human IHC-P*, IP, WB Mouse Purified 0.1 mg MCA1842
p53 Monoclonal Antibody DO-1 Bovine, Cat, Horse,
Human, Monkey IHC-F, IHC-P* Mouse Purified 10 µg MCA1701T
p53 Monoclonal Antibody DO-1 Bovine, Cat, Horse,
Human, MonkeyE, IHC-F, IHC-P*, IP, WB Mouse Purified 0.1 mg MCA1701
p53 Monoclonal Antibody DO-1 Bovine, Cat, Horse,
Human, Monkey FC* Mouse Biotin 0.1 mg MCA1701B
p53 Monoclonal Antibody DO-1 Bovine, Cat, Horse,
Human, Monkey FC* Mouse FITC 0.1 mg MCA1701F
p53 Monoclonal Antibody DO-1 Bovine, Cat, Horse,
Human, Monkey IHC-F, IHC-P* Mouse Ready to use 6 ml MCA1701H
p53 Monoclonal Antibody DO-1 Bovine, Cat, Horse,
Human, Monkey IHC-F, IHC-P* Mouse Ready to use 1 ml MCA1701HT
p53 Monoclonal Antibody DO-1 Bovine, Cat, Horse,
Human, Monkey FC* Mouse RPE 100 Tests MCA1701PE
p53 Monoclonal Antibody DO-7 Bovine, Human FC*, IHC-F,
IHC-P*, IP, WB Mouse Purified 0.1 mg MCA1703
p53 Monoclonal Antibody DO-7 Bovine, Human IHC-F, IHC-P* Mouse Ready to use 6 ml MCA1703H
p53 Monoclonal Antibody DO-7 Bovine, Human IHC-F, IHC-P* Mouse Ready to use 1 ml MCA1703HT
p53 Polyclonal Antibody Human IHC-F, IHC-P* Rabbit Ready to use 6 ml AHP535H
Find out more at: abdersotec.com/suppressorabs
Tumor Suppressor Antibodies Tumor Suppressor Antibodies
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Tumor Suppressors (Antibodies) Continued
References
Lodish H et al. (2000). Molecular Cell Biology, 4th edition. New York: WH Freeman; 2000.
Condon LT et al. (2002). Overexpression of Bcl-2 in squamous cell carcinoma of the larynx: a marker of radioresistance. Int J Cancer. 2002 Aug 1;100(4):472-5.
NHS UK. Predictive gene tests for cancer risk genes. http://www.nhs.uk/conditions/predictive-genetic-tests-cancer/Pag-es/Introduction.aspx, accessed June 08, 2015.
Vuoriluoto K et al. (2011). Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer. Oncogene. 2011 Mar 24;30(12):1436-48.
Analyzing cell proliferation? Looking to upgrade your MTT assay? The Cell Proliferation Product Portfolio literature The alamarBlue® Cell Proliferation and Viabilityprovides an overview of the cell proliferation process, Reagent literature highlights the advantages ofand summarizes how best to quantify aberrant alamarBlue and outlines how to analyze yourproliferation. experiments with the help of our online calculators.
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Specificity Product Type Clone Species Reactivity Applications Host Format Size Product Code
PTEN Tumor Supressor Protein
Monoclonal Antibody A2b1 Human IHC-F*, IP, WB Mouse Purified 0.1 mg OBT1120
Retinoblastoma Monoclonal Antibody 7F10 Human, Mouse IHC-F, WB* Rat Purified 0.1 mg MCA2104
Retinoblastoma Monoclonal Antibody 51B7 Human, Mouse IHC-F, WB* Mouse Purified 0.1 mg MCA2105
Retinoblastoma Monoclonal Antibody IF8 Human IHC-F*, IHC-P*,
WB Mouse S/N 1 ml MCA1801
Retinoblastoma Monoclonal Antibody IF8 Human IHC-F*, IHC-P* Mouse S/N 0.1 ml MCA1801T
TSC1 Polyclonal Antibody Human, Mouse, Rat IHC-F, IHC-P, WB Rabbit Purified 50 µg AHP1001T
TSC1 Polyclonal Antibody Human, Mouse, Rat IHC-F, IHC-P, WB Rabbit Purified 0.1 mg AHP1001
WRN Monoclonal Antibody 3C11 Human, Rat IF/ICC, WB Mouse Preservative
Free 0.1 mg MCA3550Z
WT1 Monoclonal Antibody 2H4 Human WB Mouse Preservative
Free 0.1 mg MCA3551Z
XPA Polyclonal Antibody Human IHC-P*, WB Rabbit Serum 0.1 ml AHP452
*Requires special treatment refer to product datasheet. Abbreviations: Alk. Phos. = Alkaline Phosphatase, AA = Amino Acids, E = ELISA, FC = Flow Cytometry, FN = Functional Assay, GSA = Gel Supershift Assay,IF/ICC = Immunofluorescence/Immunocytochemistry, IHC-F = Immunohistochemistry - frozen (Cryostat), IHC-P = Immunohistochemistry - paraffin, IP = Immunoprecipitation, Pur. = Purified, Rec. = Recombinant, S/N = Supernatant, WB = Western Blotting.
Bio-Rad Laboratories, Inc.
LIT.OCT. V1. 2015 © Copyright Bio-Rad Laboratories, Inc. All rights reserved. Published by AbD Serotec, a Bio-Rad Company, Endeavour House, Langford Lane, Langford Business Park, Kidlington, OX5 1GE. AbD Serotec reagents are for research purposes only, not for therapeutic or diagnostic use. alamarBlue® is manufactured for AbD Serotec by Trek Diagnostic System. U.S. patent 5,501,959. Alexa Fluor® is a registered trademark of Molecular Probes Inc. OR, USA. DyLight® is a trademark of Thermo Fisher Scientific Inc, and its subsidaries. HuCAL® is a registered trademark of MorphoSys AG.
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