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Wednesday, February 9, 2011

Objective: Students will be able to sequence the steps involved in protein synthesis.

Bellringer: What is the difference between protein

synthesis and DNA replication?

4 Minutes Remaining…

Wednesday, February 9, 2011

Objective: Students will be able to sequence the steps involved in protein synthesis.

Bellringer: What is the difference between protein

synthesis and DNA replication?

3 Minutes Remaining…

Wednesday, February 9, 2011

Objective: Students will be able to sequence the steps involved in protein synthesis.

Bellringer: What is the difference between protein

synthesis and DNA replication?

2 Minutes Remaining…

…and you think you look weird when you sneeze…

Wednesday, February 9, 2011last chance to view BR - 1 min

Objective: Students will be able to sequence the steps involved in protein synthesis.

Bellringer: What is the difference between protein

synthesis and DNA replication?

1 Minute Remaining…

30 Seconds Remaining…

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Turn In Your Bellringer!

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Agenda

1. Bellringer2. Reminder: Assignments Due List3. Finish Notes4. Work Time: Problem Set & Old

Assignments

Tomorrow: DNA Lab

So far this semester, you should have completed…

I will be entering in these grades, so get these in please!

Reminder: Assignments List

Day Assigned Date AssignmentMonday 1/31 BrainPop DNATuesday 2/1 DNA OrigamiMonday 2/7 MiniLab 11.1 (p. 293)Tuesday 2/8 Quiz – Chapter 11Tuesday 2/8 DNA & RNA Webquest

Wednesday 2/9 DNA & RNA Problem Set

DNA VOCAB. – Test yourself!

1. DNA2. Nitrogenous

base3. Double helix4. DNA replication5. Nucleotide6. Purines7. Pyrimidines8. Guanine (G)9. Adenine (A)10.Cytosine (C)11.Thymine (T)

A. Carbon ring structure contains 1+ Nitrogen, includes: G, A, C, T

B. Subunit of nucleic acidC. This purine base pairs with

ThymineD. Deoxyribonucleic acid: contains

genes E. This pyrimidine base pairs with

AdenineF. DNA is copiedG. This purine base pairs with

CytosineH. This pyrimidine base pairs with

GuanineI. 2 strands of DNA twisted togetherJ. Contains a double nitrogen ringK. Contains a single nitrogen ring

RNA VOCAB. – Write these down!

1. Transcription2. Translation3. Codon4. Anti-Codon5. RNA6. rRNA7. mRNA8. tRNA

A. “Ribosomal RNA” makes up ribosome & provides site for protein synthesis

B. “Transfer RNA” carries anti-codon & amino acid (looks like a t)

C. Ribonucleic acid; protein synthesis molecules

D. “Messenger RNA” carries protein synthesis directions to ribosome

E. DNA codes into mRNA molecule F. Anti-codon pairs with codon &

amino acids link togetherG. 3-nucleotide unit of mRNAK. Pairs with codon, attached to

tRNA

Identify the structures….

DNA Base pairs:A – TC – G

• Held together by hydrogen bonds (weak)

• Secured to a sugar-phosphate backbone

DNA ReplicationGoal:

DNA is copied. Pairs:

A – TC – G

Steps: 1. DNA “unzips”2. Complimentary free

nucleotides attach to sides of “zipper” to form new DNA strand

Practice Replication Now: A T C G T G C A T - - - - - - - - -

T A G C A C G T A

Protein Synthesis

Key players in creating protein1. DNA - contains

directions2. mRNA - carries

directions from nucleus through cytoplasm to the ribosome

3. rRNA - binds to the mRNA & uses instructions to assemble AA in order

4. tRNA - delivers amino acid to ribosome for assembly into a protein

Transcription occurs in the NUCLEUS

Steps =1. Double helix

unwinds2. DNA “unzips”3. Complimentary

free nucleotides attach to sides of “zipper” to form a strand of mRNA

No Thymine is available in RNA, it is replaced by

URACIL (U). (No more T… A – U or U – A)

mRNA is read in Codons

Translation occurs in the RIBOSOME

Steps = 1. mRNA & tRNAs

carrying amino acids meet at ribosome

2. tRNA triplet anti-codon matches mRNA codon

3. #2 repeats & amino acids chain together until entire mRNA strand is translated into proteinRemember: No Thymine is available in

RNA, it is replaced by URACIL (U). (No more T… A – U or U – A)

Anti-Codons & Translation

Review the steps of Protein Synthesis

From the next 3 slides, pick your favorite

mRNA amino acid chart

1. Practice the translation process and determine the amino acid from these mRNA sequences:UCAAACCGAGUACAG

My favorite. Time to practice…Practice the translation

process and determine the amino acid from these mRNA sequences:

• UCA• AAC• CGA• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC• CGA• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA =

ARGININE• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA =

ARGININE• GUA = VALINE• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA =

ARGININE• GUA = VALINE• CAG =

GLUTAMINE

Not my fave, but time to practice…Practice the translation

process and determine the amino acid from these mRNA sequences:

• UCA• AAC• CGA• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC• CGA• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA =

ARGININE• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA =

ARGININE• GUA = VALINE• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA =

ARGININE• GUA = VALINE• CAG =

GLUTAMINE

Just discovered this one. Time to practice…Practice the translation

process and determine the amino acid from these mRNA sequences:

• UCA• AAC• CGA• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC• CGA• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA =

ARGININE• GUA• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA =

ARGININE• GUA = VALINE• CAG

Practice the translation process and determine the amino acid from these mRNA sequences:

• UCA = SERINE• AAC =

ASPARAGINE• CGA =

ARGININE• GUA = VALINE• CAG =

GLUTAMINE

This is the BEST transcription/translation website ever. Try IT

now!http://learn.genetics.utah.edu/content/begin/dna/transcribe/

MAKE THE DNA REPLICATE

C C T A A G A G C G G C

You create the new DNA

Original DNA

MAKE THE DNA REPLICATE

C C T

G G A T T C T C G C C G

A A G A G C G G C

Original DNA

New DNA

TRANSCRIBE THE DNA CODE INTO mRNA

T C G G A C A T C C G T

DNA

You create the mRNA

TRANSCRIBE THE DNA CODE INTO mRNA

T C G

A G C C U G U A G G C A

G A C A T C C G T

DNA

mRNA

USE THE mRNA CODE PROVIDEDTO ADD THE COMPLEMENTARY tRNA

U C G

A G C C U G U A G G C A

A U CG A C U A C

mRNA

tRNA AMINO ACID

USE THE mRNA & tRNA TO TRANSCRIBE THE PROTEIN CHAIN

G A C A U C C G U

A C GA G C C U G U A G G C A

mRNA

tRNA

AMINO ACID

USE THE mRNA & tRNA TO TRANSCRIBE THE PROTEIN CHAIN

A U C

G A C

C G U

U C G

mRNA

tRNA

A G C C U G U A G G C A

AMINO ACID

USE THE mRNA & tRNA TO TRANSCRIBE THE PROTEIN CHAIN

C G U

G A C A U C

mRNA

tRNA

A G C C U G U A G G C A

AMINO ACIDPROTEIN CHAIN

USE THE mRNA & tRNA TO TRANSCRIBE THE PROTEIN CHAIN

C G UA U C

mRNA

tRNA

A G C C U G U A G G C A

AMINO ACIDPROTEIN CHAIN

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located?2. Where does REPLICATION occur?3. The difference between replication & protein synthesis

is ____________________________.4. What molecule chain is formed during transcription?5. Once that molecule is formed from where, to where,

does the molecule travel?6. mRNA is read in 3-nucleotide segments known as

_______.7. Once at the ribosome, the last step of protein

synthesis, _________ occurs.8. ______ has an anti-codon at the base which pairs with

each codon on the mRNA. 9. An amino acid is present at the top of tRNA. Amino

acids begin to link together to form a _____________.

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? 3. The difference between replication & protein synthesis is4. What molecule chain is formed during transcription? 5. Once that molecule is formed from where, to where, does the

molecule travel? 6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,

__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on

mRNA. 9. An amino acid is present at the top of tRNA. Amino acids

begin to link together to form a ________________.

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is4. What molecule chain is formed during transcription? 5. Once that molecule is formed from where, to where, does the

molecule travel? 6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,

__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on

mRNA. 9. An amino acid is present at the top of tRNA. Amino acids

begin to link together to form a ________________.

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is

REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.

4. What molecule chain is formed during transcription? 5. Once that molecule is formed from where, to where, does the

molecule travel? 6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,

__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on

mRNA. 9. An amino acid is present at the top of tRNA. Amino acids

begin to link together to form a ________________.

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is

REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.

4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the

molecule travel? 6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,

__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on

mRNA. 9. An amino acid is present at the top of tRNA. Amino acids

begin to link together to form a ________________.

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is

REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.

4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the

molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME

6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,

__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on

mRNA. 9. An amino acid is present at the top of tRNA. Amino acids

begin to link together to form a ________________.

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is

REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.

4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the

molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME

6. mRNA is read in 3-nucleotide segments known as CODONS.7. Once at the ribosome, the last step of protein synthesis,

__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on

mRNA. 9. An amino acid is present at the top of tRNA. Amino acids

begin to link together to form a ________________.

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is

REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.

4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the

molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME

6. mRNA is read in 3-nucleotide segments known as CODONS.7. Once at the ribosome, the last step of protein synthesis,

TRANSCRIPTION occurs.8. ____ has an anti-codon at the base that pairs with a codon on

mRNA. 9. An amino acid is present at the top of tRNA. Amino acids

begin to link together to form a ________________.

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is

REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.

4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the

molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME

6. mRNA is read in 3-nucleotide segments known as CODONS.7. Once at the ribosome, the last step of protein synthesis,

TRANSCRIPTION occurs.8. .tRNA has an anti-codon at the base that pairs with a codon

on mRNA. 9. An amino acid is present at the top of tRNA. Amino acids

begin to link together to form a ________________.

LET’S REVIEW THE KEY POINTS:

1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is

REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.

4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the

molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME

6. mRNA is read in 3-nucleotide segments known as CODONS.7. Once at the ribosome, the last step of protein synthesis,

TRANSCRIPTION occurs.8. .tRNA has an anti-codon at the base that pairs with a codon

on mRNA. 9. An amino acid is present at the top of tRNA. Amino acids

begin to link together to form a PROTEIN (POLYPEPTIDE) CHAIN.