Regents Biology

Microscopes!

Regents Biology

Mutual dependence: Science & Technology

Technology…the pragmatist

Does it work?

Is it useful?

Is it sustainable?

Science…the idealist

What is it?

How does it work?

What does it tell us?

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Mutual dependence: Science & Technology

Microscopes

Electron microscopes

Optical (light) microscopes

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Size matters micron = micrometer = 1/1,000,000 meter

diameter of human hair = ~20 microns

Miss N, Show them the interactive!!

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Microscopic units of measurement

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1 metre (m) = ________ millimetres (mm)

1 mm = ________ microns (µm)

1 µm = ________ nanometres (nm)

1 mm = ________ nm

1 m = ________ µm

1 m =________ nm

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1 m = 1,000 mm = 103mm

1 mm = 1,000 µm= 103µm

1 µm = 1,000 nm= 103nm

1 mm = 1,000,000 nm = 106nm

1 m = 1,000,000 µm = 106µm

1 m = 1,000,000,000nm =109 nm

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10-3 m = ________

10-6 m = ________

10-9 m = ________

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10-3 m = 0.001m = 1 mm

10-6 m = 0.000001 = 1 µm

10-9 m = 0.000000001= 1nm

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Electron microscope range:

_____ to _____

Light microscope range:

_____ to _____

Unaided human eye range:

_____ to _____Unaided?

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Electron microscope range:

1 nm to 100 µm

Light microscope range:

1 µm to 1 mm

Unaided human eye range:

1 mm upwards

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Name that image

Light microscope

or

Electron microscope?

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Regents Biology19 Light microscope

Unstained

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Regents Biology20 Light microscope

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Regents Biology22 Light microscope

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Tick

Regents Biology23 Electron microscope

Tick

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Regents Biology24 Electron microscope

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Regents Biology25 Light microscope

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Regents Biology26 Electron microscope

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Aphid

Regents Biology27 Electron microscope

Aphid

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Regents Biology28 Light microscope

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Regents Biology29 Light microscope

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Regents Biology30 Electron microscope

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Regents Biology31 Electron microscope

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Regents Biology32 Electron microscope

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Regents Biology33 Electron microscope

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Regents Biology35 Light microscope

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Regents Biology36 Light microscope

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Regents Biology37 Light microscope

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THE END!

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Magnification

Magnification = ocular lens x objective lens

A microscope has an eyepiece lens with a power

of 20X. The objective lens being used has a

power of 10X. What is the total magnification?

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Using Scale Bars

Using a scale bar:The image at right is of a virus-like

particle. The bar represents 50nm.

Calculate the actual size of the

image. 50nm

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1. Measure the scale bar

50nm=1cm

2. Measure the image

Let’s say it was 2cm.

Actual size: Image size = constant

50:1 = X:2

X=2*50

=100nm

Using a scale bar:

50nm

Using Scale Bars

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The image at right is of a virus-

like particle. The bar represents

50nm.

Calculate the magnification.

50nm

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Magnification: I AM

Watch yo units!!

Image size

Actual size Magnification

Measure with ruler Need to figure this out

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1. Measure the size of the image.

…..2cm (20mm)

2. Find the actual size of the sample

…..100nm Or 1.0 x 102

3. Convert measured size into unit on scale bar.

(in this case, nm)

mm µm 1mm = 1000 µm

20 x 1000µm =20,000 µm

µm nm 1 µm = 1000 nm

20,000 x 1000nm = 20,000,000nm OR 2.0 x 107nm

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Magnification: I AM

Watch yo units!!

Image size

Actual size Magnification

2.0 x 107Need to figure this out

1.0 x 102

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(2.0x107) / (1.0x102)

=2.0 x 10(7-2)

=2.0 x 105

x200,000 whoa50nm

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Magnification: I AM

Watch yo units!!

Image size

Actual size Magnification

2.0 x 107nm

X 2.0 x 105

1.0 x 102nm

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Using Microscopes