Loschmidt Laboratories

Enantis, Ltd.

PROTEIN ENGINEERING

LOSCHMIDT LABORATORIES & ENANTIS

17 years experience in protein science

multi-disciplinary team

LOSCHMIDT LABORATORIES & ENANTIS

17 years experience in protein science

multi-disciplinary team

laboratories and facilities (1.5 mil EUR)

University Campus and Biotechnology Incubator, Brno

LOSCHMIDT LABORATORIES & ENANTIS

17 years experience in protein science

multi-disciplinary team

laboratories and facilities (1.5 mil EUR)

University Campus and Biotechnology Incubator, Brno

molecular biology and microbiology

LOSCHMIDT LABORATORIES & ENANTIS

17 years experience in protein science

multi-disciplinary team

laboratories and facilities (1.5 mil EUR)

University Campus and Biotechnology Incubator, Brno

molecular biology and microbiology

bioinformatics and molecular modelling

17 years experience in protein science

multi-disciplinary team

laboratories and facilities (1.5 mil EUR)

University Campus and Biotechnology Incubator, Brno

molecular biology and microbiology

bioinformatics and molecular modelling

biochemistry and biophysics

LOSCHMIDT LABORATORIES & ENANTIS

LOSCHMIDT LABORATORIES & ENANTIS

BIOSENSING

DECONTAMINATION

BIOCATALYSIS

DEVELOPMENT OF BIOCATALYTIC PROCESS

GENETIC DATABASES

EXTREMOPHILES

DEVELOPMENT OF BIOCATALYTIC PROCESS

DIRECTED EVOLUTION

resistance to organic solvents

DEVELOPMENT OF BIOCATALYTIC PROCESS

DIRECTED EVOLUTION

RATIONAL DESIGN

thermostability enhancement

DEVELOPMENT OF BIOCATALYTIC PROCESS

DIRECTED EVOLUTION

RATIONAL DESIGN

thermostability enhancement

DEVELOPMENT OF BIOCATALYTIC PROCESS

DIRECTED EVOLUTION

RATIONAL DESIGN

thermostability enhancement

0

0.2

0.4

0.6

0.8

1

1.2

30 40 50 60 70 80

Nat

ive

stru

ctur

e

Temperature [°C]

20°C

DEVELOPMENT OF BIOCATALYTIC PROCESS

OPTIMIZED EXPRESSION

FERMENTATION

DOWN STREAM

21.5

29.0

35.4

52.9

92.0113.0

1 2 3 4

DhaA-LinB

DEVELOPMENT OF BIOCATALYTIC PROCESS

MEDIUM ENGINEERING

IMMOBILISATION

DEVELOPMENT OF BIOCATALYTIC PROCESS

HOTSPOT WIZARD

Hot Spot Wizard

RCSB PDB

user

Hot Spot Wizard

RCSB PDB

CSAUniProt

user

PDBSWS

HOTSPOT WIZARD

Hot Spot Wizard

RCSB PDB

CSAUniProt

user CAVER

CASTp

PDBSWS

HOTSPOT WIZARD

Hot Spot Wizard

RCSB PDB

CSAUniProt

user CAVER

CASTp

PDBSWS

tunnels

Cd-hit

BLAST NRDB

MUSCLERate4Site

HOTSPOT WIZARD

low highmutability

HOTSPOT WIZARD

1,3-1,4--glucanase PDB-ID 1GBG

haloalkane dehalogenasePDB-ID 1BN6

phosphotriesterasePDB-ID 1I0D

-lactamasePDB-ID 1BLS

HOTSPOT WIZARD

Estimated mutability

hot spotsregion

Pro

po

rtio

n o

f m

uta

tio

ns

HOTSPOT WIZARD

positive and neutral

Estimated mutability

hot spotsregion

Pro

po

rtio

n o

f m

uta

tio

ns

HOTSPOT WIZARD

positive and neutral slightly negative

positive and neutral slightly negative deleterious

Estimated mutability

hot spotsregion

Pro

po

rtio

n o

f m

uta

tio

ns

HOTSPOT WIZARD

HOTSPOT WIZARD

Pavelka et al. Nucl. Acids Res. 37: W376 2009