Transcript of LLNL-PRES-573032 This work was performed under the auspices of the U.S. Department of Energy by...
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- LLNL-PRES-573032 This work was performed under the auspices of
the U.S. Department of Energy by Lawrence Livermore National
Laboratory under contract DE-AC52-07NA27344. Lawrence Livermore
National Security, LLC Stavros G. Demos, Ph.D. Physicist, LLNL
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Autofluorescence Capturing UV Microscope Normal Esophagus Barretts
Esophagus In vivo imaging of tissues microstructure Instantaneous
diagnostic decision-making Non-invasive Reduces overall time spent
in surgery Eliminates anxiety in waiting for diagnosis Reduces
overall costs Greatly improves healthcare
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- Lawrence Livermore National Laboratory LLNL-PRES-573032 Tissue
sample is removed from the patient, sent to the lab, sliced into
sections, mounted on slides, stained, and examined under microscope
1.Invasive 2.Results in multiple and repetitive surgeries
3.Imprecise 4.Random sampling error often misses pre-cancerous
cells 5.Feedback takes extended amounts of time, (typically days)
6.Costs are extremely high 3 Source: murrasaca.com
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Endomicroscope systems Instantaneous/real-time diagnostic info. for
surgeon Early, instant, accurate detection of disease (cancer,
etc.) Imaging helps guide surgical intervention 1.Noninvasive
2.Reduces repetitive surgeries 3.Precise cellular level images
4.Random sampling error is not a factor 5.Feedback is instantaneous
6.Drastically reduces overall costs 4 Source:
www.gastroarkansas.com
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Source: maunakeatech.com Mauna Keas Confocal Laser Endomicroscopy
product: *optical sectioning *fluorescein
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- Lawrence Livermore National Laboratory LLNL-PRES-573032
Visualization at the cellular level Details of epithelial
microstructure Visualization of the margins Targeted biopsies High
resolution images (spatial resolution ~ 1 m) 7 Gold Standard:
Tissue biopsy H&E Stain AF capturing UV Microscope: Real Time
Epithelium of Stomach Features seen are comparable to Gold
Standard, BUT AF capturing UV microscope provides greater speed,
ease, & cost efficiency
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Heeryae.com Microscopy of the lumens of several organs Laparoscopic
microscopy & Hand-held microscopy In vivo pathology markets
Similar to frozen section biopsy without tissue processing, (all
done in OR) Ex vivo market Visualization of cellular biochemistry
& molecular function Cellular level view of drug delivery,
targeting, cellular response, metabolism, and drug removal
Pharmaceutical Industry In vivo study of tumors, tissue
abnormalities, etc. Laboratory Animal Research In vivo
lessons/study of the GI tract, organs, tissues, and cellular level
features University and Secondary Bio/Anatomy Edu.
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Available tabletop prototype Tested microendoscope concept and
design parameters (ex vivo) Diagnostic studies: human esophageal
tissues and animal tissues (ex vivo) Animal imaging and stem cell
localization/targeting Future Development: Proof of Principle in
Humans (in vivo) 9 Source: businessinsider.com 1 Construct
pre-product microendoscope 2 Validation using human subjects 3
Commercialization
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- Lawrence Livermore National Laboratory LLNL-PRES-573032 Genaro
Mempin mempin1@llnl.gov 10